BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0397.0026.p (268 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value TM1469 [KG] COG1940 Transcriptional regulator/sugar kinase 80 5e-15 SP1675 [KG] COG1940 Transcriptional regulator/sugar kinase 75 7e-14 BS_glcK [KG] COG1940 Transcriptional regulator/sugar kinase 74 3e-13 BH0797 [KG] COG1940 Transcriptional regulator/sugar kinase 69 5e-12 SPy0258 [KG] COG1940 Transcriptional regulator/sugar kinase 68 1e-11 >TM1469 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 317 Score = 79.7 bits (195), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 61/269 (22%), Positives = 105/269 (39%), Gaps = 17/269 (6%) Query: 6 IGADIGGTGIKAAVIDERGGIITRSSRPTPVADGAKGILLALKGVISGLLADGPEVSGIG 65 IG D+GGT ++ E G I+ + +R T V +G + ++ + I ++DG E +G Sbjct: 7 IGVDLGGTTFSVGLVSEDGKILKKVTRDTLVENGKEDVIRRIAETILE-VSDGEEAPYVG 65 Query: 66 IGTAGRVDPEEGVVLYATDNLPGWTGMRLAAEIEAEFXXXXXXXXXXXXXXXXXXXXXXX 125 IG+ G +D E G+V ++ N P W + L E+ Sbjct: 66 IGSPGSIDRENGIVRFSP-NFPDWHNVPLTDELAKRTGKKVFLENDANAFVLGEKWFGAG 124 Query: 126 XXXXDYAMXXXXXXXXXXXXHKNQPVSGKQGAAGEFGHMVLYPGGIPCSCGQSGCTEHYL 185 ++G+ G E GH+V+ P G C+CG GC E Sbjct: 125 RGHDHIVALTLGTGIGGGVVTHGYLLTGRDGIGAELGHVVVEPNGPMCNCGTRGCLEAVA 184 Query: 186 SGRALNRL---------------AAGANEGWDSRRLLXXXXXXXXXXXXVMEVYMNDLSL 230 S A+ R AG+ E D++ L + + ++ L+ Sbjct: 185 SATAIRRFLREGYKKYHSSLVYKLAGSPEKADAKHLFDAARQGDRFALMIRDRVVDALAR 244 Query: 231 TVHNIQSFFDPGAIILGGGVAESHPLWWG 259 V F+P +I+GGG++ + + +G Sbjct: 245 AVAGYIHIFNPEIVIIGGGISRAGEILFG 273 >SP1675 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 294 Score = 75.5 bits (184), Expect = 7e-14, Method: Compositional matrix adjust. Identities = 36/98 (36%), Positives = 54/98 (55%), Gaps = 1/98 (1%) Query: 5 YIGADIGGTGIKAAVIDERGGIITRSSRPTPVADGAKGILLALKGVISGLLADGPEVSGI 64 Y+ DIGGT IK ++D+ G ++ PT G IL K +++ L GP V+G+ Sbjct: 4 YVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQKTKDIVASYLEKGP-VAGV 62 Query: 65 GIGTAGRVDPEEGVVLYATDNLPGWTGMRLAAEIEAEF 102 I +AG VDP++G + YA +P + G + EIE F Sbjct: 63 AISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESF 100 >BS_glcK [KG] COG1940 Transcriptional regulator/sugar kinase Length = 321 Score = 73.6 bits (179), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 71/267 (26%), Positives = 102/267 (38%), Gaps = 17/267 (6%) Query: 5 YIGADIGGTGIKAAVIDERGGIITRSSRPTPVADGAKGILLA--LKGVISGLLADGPEVS 62 + G D+GGT IK A I++ G I + PT + +A + + L + Sbjct: 6 FAGIDLGGTTIKLAFINQYGEIQHKWEVPTDKTGDTITVTIAKTIDSKLDELQKPKHIIK 65 Query: 63 GIGIGTAGRVDPEEGVVLYATDNLPGWTGMRLAAEIEAEFXXXXXXXXXXXXXXXXXXXX 122 IG+G G VD GVV Y T NL GW L +E E Sbjct: 66 YIGMGAPGPVDMAAGVV-YETVNL-GWKNYALKNHLETETGIPAVIENDANIAALGEMWK 123 Query: 123 XXXXXXXDYAMXXXXXXXXXXXXHKNQPVSGKQGAAGEFGHMVLYP-GGIPCSCGQSGCT 181 D + + + G GA GE GH+ P GG PC+CG++GC Sbjct: 124 GAGDGAKDVILVTLGTGVGGGIIANGEILHGINGAGGEIGHICSIPEGGAPCNCGKTGCI 183 Query: 182 EHYLSGRALNRLAAG------------ANEGWDSRRLLXXXXXXXXXXXXVMEVYMNDLS 229 E S + R+A G A E +R + V++ L Sbjct: 184 ETIASATGIVRIAKGKIANAKKTTRLKATEQLSARDVFEAAGENDEIALEVVDYVAKHLG 243 Query: 230 LTVHNIQSFFDPGAIILGGGVAESHPL 256 L + N+ S +P I+LGGGV+ + L Sbjct: 244 LVLGNLASSLNPSKIVLGGGVSRAGEL 270 >BH0797 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 293 Score = 69.3 bits (168), Expect = 5e-12, Method: Compositional matrix adjust. Identities = 65/258 (25%), Positives = 100/258 (38%), Gaps = 15/258 (5%) Query: 6 IGADIGGTGIKAAVIDERGGIITRSSRPTPVADGAKGILLALKGVISGLLADGPEVSGIG 65 +G D+GGT IKAA++ + G II+ PT A G + ++ + + + P +GIG Sbjct: 3 VGIDLGGTKIKAALVSDAGEIISVQECPTEAAQGPEEVMNKMMSLTEKVTDHQP-FAGIG 61 Query: 66 IGTAGRVDPEEGVVLYATDNLPGWTGMRLAAEIEAEFXXXXXXXXXXXXXXXXXXXXXXX 125 IG G + EG +L + NLPGW + L + +F Sbjct: 62 IGAPGPLSSTEGTIL-SPPNLPGWDHIHLVDRFQEQFQCPVKLDNDANVAALAEALLGSG 120 Query: 126 XXXXDYAMXXXXXXXXXXXXHKNQPVSGKQGAAGEFGHMVLYPGGIPCSCGQSGCTEHYL 185 V G AGE G+M++ P G + G E Sbjct: 121 QGFTSVFYLTISTGIGGGYVLDGSIVHGASDYAGEIGNMIVQPNGYQHANLNPGSLEGLA 180 Query: 186 SGRALNRLA-------AGANEGWDSRRLLXXXXXXXXXXXXVMEVYMNDLSLTVHNIQSF 238 SG A+ R+A G E +D R ++E M+ L++ + NI Sbjct: 181 SGTAIGRMARERFGVEGGTREVFDQIR------RGDHDMQRLVEEAMDYLAIGIANIAHT 234 Query: 239 FDPGAIILGGGVAESHPL 256 +P +LGGGV + L Sbjct: 235 INPDVFVLGGGVMNADDL 252 >SPy0258 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 312 Score = 68.2 bits (165), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 34/100 (34%), Positives = 54/100 (54%), Gaps = 1/100 (1%) Query: 3 RCYIGADIGGTGIKAAVIDERGGIITRSSRPTPVADGAKGILLALKGVISGLLADGPEVS 62 + Y+ DIGGT IK +I E G ++ + T G IL +KG++ D +++ Sbjct: 2 KHYLAIDIGGTAIKYGLISETGDLLEKEEMATEAYKGGPSILEKVKGLVK-TYQDQMDLA 60 Query: 63 GIGIGTAGRVDPEEGVVLYATDNLPGWTGMRLAAEIEAEF 102 G+ I +AG V+P+EG + YA +P + G + EIE F Sbjct: 61 GVAISSAGMVNPDEGEIFYAGPQIPNYAGTQFKKEIEETF 100 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.139 0.444 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,315,583 Number of extensions: 655433 Number of successful extensions: 2832 Number of sequences better than 1.0e-05: 39 Number of HSP's gapped: 2815 Number of HSP's successfully gapped: 44 Length of query: 268 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 163 Effective length of database: 26,268,361 Effective search space: 4281742843 Effective search space used: 4281742843 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)