BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0397.0025.p (238 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SP1330 [G] COG3010 Putative N-acetylmannosamine-6-phosphate epim... 145 6e-35 SP1685 [G] COG3010 Putative N-acetylmannosamine-6-phosphate epim... 130 2e-30 STM3337 [G] COG3010 Putative N-acetylmannosamine-6-phosphate epi... 111 1e-24 STM1129 [G] COG3010 Putative N-acetylmannosamine-6-phosphate epi... 107 2e-23 >SP1330 [G] COG3010 Putative N-acetylmannosamine-6-phosphate epimerase Length = 233 Score = 145 bits (366), Expect = 6e-35, Method: Compositional matrix adjust. Identities = 81/218 (37%), Positives = 123/218 (56%), Gaps = 5/218 (2%) Query: 13 GLVVSCQALEDEPLHGSXXXXXXXXXXXXXXXXX--IRAGGLEDIRAIKEAVGLPLIGLV 70 G++VSCQAL E L+ IRA + DI+AI++ V LP+IG++ Sbjct: 17 GVIVSCQALPGEALYNEEFSLMPFMAKAALEAGAVGIRANSVRDIKAIQKVVDLPIIGII 76 Query: 71 KIRHENSEVYITPTLEDALAVHAAGADIVAIDGTGRPRPDGRSLADTIAGLKRA--GAAV 128 K + E YIT T+++ + G ++A D T RPR DG +++ I +K + Sbjct: 77 KRDYPPQEPYITATMKEVDELVECGTTVIAFDATLRPRYDGLVVSEFIKKIKEKYPNQLL 136 Query: 129 MADIATFEEGVLAAERGADYVSTTLCGYTRETAGTPLPNLGLLERLTAVLTIPVVAEGGI 188 MAD++ +EG+ A + G D+V TTL GYT + + P+ L+++L A IPV+AEG I Sbjct: 137 MADVSNLDEGLYAFKSGVDFVGTTLSGYTSTSVQSDEPDFELMKKL-ADFNIPVIAEGKI 195 Query: 189 CQPEQAAEALRLGAAFVVVGAAITRPQWIAARYVEAMQ 226 PEQ +A LG VV+G AITRP+ IA R++ ++ Sbjct: 196 HYPEQLKKAYSLGVTSVVIGGAITRPKEIAQRFINVIK 233 >SP1685 [G] COG3010 Putative N-acetylmannosamine-6-phosphate epimerase Length = 232 Score = 130 bits (327), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 79/218 (36%), Positives = 118/218 (54%), Gaps = 5/218 (2%) Query: 13 GLVVSCQALEDEPLHGSXXXXXXXXXXXXXX--XXXIRAGGLEDIRAIKEAVGLPLIGLV 70 G++VSCQAL EPL+ IRA + DI+ IKE LP+IG++ Sbjct: 16 GIIVSCQALPHEPLYTEAGGVIPLLVKAAEQGGAVGIRANSVRDIKEIKEVTKLPIIGII 75 Query: 71 KIRHENSEVYITPTLEDALAVHAAGADIVAIDGTGRPRPDGRSLADTIAGLKRA--GAAV 128 K + E +IT T+++ + +++A+D T R R DG + + I +K + Sbjct: 76 KRDYPPQEPFITATMKEVDELAELDIEVIALDCTKRERYDGLEIQEFIRQVKEKYPNQLL 135 Query: 129 MADIATFEEGVLAAERGADYVSTTLCGYTRETAGTPLPNLGLLERLTAVLTIPVVAEGGI 188 MAD + FEEG+ A E G D+V TTL GYT + P+ L+++L + V+AEG I Sbjct: 136 MADTSIFEEGLAAVEAGIDFVGTTLSGYTSYSPKVDGPDFELIKKLCDA-GVDVIAEGKI 194 Query: 189 CQPEQAAEALRLGAAFVVVGAAITRPQWIAARYVEAMQ 226 PEQA + L G +VVG AITRP+ I R+V +++ Sbjct: 195 HTPEQAKQILEYGVRGIVVGGAITRPKEITERFVASLK 232 >STM3337 [G] COG3010 Putative N-acetylmannosamine-6-phosphate epimerase Length = 229 Score = 111 bits (277), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 77/215 (35%), Positives = 115/215 (53%), Gaps = 6/215 (2%) Query: 13 GLVVSCQALEDEPLHGSXXXXXXXXXXXXXXXXXIRAGGLEDIRAIKEAVGLPLIGLVKI 72 GL+VSCQ + PL +R G++++R + V +P+IG++K Sbjct: 16 GLIVSCQPVPGSPLDKPEIVAAMALAAEQAGAVAVRIEGIDNLRMTRSLVSVPIIGIIKR 75 Query: 73 RHENSEVYITPTLEDALAVHAAGADIVAIDGTGRPRPDGRSLADTIAGLKRAGAAVMADI 132 + S V ITP L+D A+ AGA I+A+DGT R RP ++ +A + MAD Sbjct: 76 DLDESPVRITPFLDDVDALAQAGAAIIAVDGTARQRP--VAVEALLARIHHHHLLAMADC 133 Query: 133 ATFEEGVLAAERGADYVSTTLCGYTRETAGTP-LPNLGLLERLTAVLTIPVVAEGGICQP 191 ++ ++G+ GAD + TT+ GYT T TP P+L L++ L V+AEG P Sbjct: 134 SSVDDGLACQRLGADIIGTTMSGYT--TPDTPEEPDLPLVKALHDA-GCRVIAEGRYNSP 190 Query: 192 EQAAEALRLGAAFVVVGAAITRPQWIAARYVEAMQ 226 AAEA+R GA V VG+AITR + I Y +A++ Sbjct: 191 ALAAEAIRYGAWAVTVGSAITRLEHICGWYNDALK 225 >STM1129 [G] COG3010 Putative N-acetylmannosamine-6-phosphate epimerase Length = 226 Score = 107 bits (267), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 74/216 (34%), Positives = 115/216 (53%), Gaps = 8/216 (3%) Query: 13 GLVVSCQALEDEPLHGSXXXXXXXXXXXXXXXXXIRAGGLEDIRAIKEAVGLPLIGLVKI 72 GL+VSCQ + P+ +R G+E++R ++ + +P+IG++K Sbjct: 16 GLIVSCQPVPGSPMDKPEIVAAMAQAAASAGAVAVRIEGIENLRTVRPHLSVPIIGIIKR 75 Query: 73 RHENSEVYITPTLEDALAVHAAGADIVAIDGTGRPRP-DGRSLADTIAGLKRAGAAVMAD 131 S V ITP L+D A+ AGADI+A D + R RP D SL + ++ G MAD Sbjct: 76 DLTGSPVRITPYLQDVDALAQAGADIIAFDASFRSRPVDIDSL---LTRIRLHGLLAMAD 132 Query: 132 IATFEEGVLAAERGADYVSTTLCGYTRETAGTPL-PNLGLLERLTAVLTIPVVAEGGICQ 190 +T EG+ ++G +++ TTL GYT TP+ P+L ++ +L+ V+AEG Sbjct: 133 CSTVNEGISCHQKGIEFIGTTLSGYTGPI--TPVEPDLAMVTQLSHA-GCRVIAEGRYNT 189 Query: 191 PEQAAEALRLGAAFVVVGAAITRPQWIAARYVEAMQ 226 P AA A+ GA V VG+AITR + I + A++ Sbjct: 190 PALAANAIEHGAWAVTVGSAITRIEHICQWFSHAVK 225 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.135 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,918,584 Number of extensions: 634381 Number of successful extensions: 1690 Number of sequences better than 1.0e-05: 5 Number of HSP's gapped: 1700 Number of HSP's successfully gapped: 5 Length of query: 238 Length of database: 38,378,431 Length adjustment: 103 Effective length of query: 135 Effective length of database: 26,499,029 Effective search space: 3577368915 Effective search space used: 3577368915 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 114 (48.5 bits)