BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0397.0011.p (420 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SP1897 [G] COG1653 ABC-type sugar transport system, periplasmic ... 150 3e-36 lin0762 [G] COG1653 ABC-type sugar transport system, periplasmic... 94 5e-19 BS_yurO [G] COG1653 ABC-type sugar transport system, periplasmic... 84 3e-16 PH1214 [G] COG1653 ABC-type sugar transport system, periplasmic ... 77 7e-14 ycjN [G] COG1653 ABC-type sugar transport system, periplasmic co... 74 3e-13 >SP1897 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 419 Score = 150 bits (380), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 113/386 (29%), Positives = 181/386 (46%), Gaps = 24/386 (6%) Query: 10 VSLLAMSLLSACGNNSGSNAATDDGGNAASGEKVKIEFFQNKTEAKATFDKLVAQFNAAN 69 + LLA + L+ G + SN G +A G V IE+F K E T +++ F N Sbjct: 7 IGLLATTGLALFGLGACSNY-----GKSADG-TVTIEYFNQKKEMTKTLEEITRDFEKEN 60 Query: 70 PDIVVSQINPPDAETVLKTRAAKQDLPDVIGLGATDT-YKTLSASGLFEDFTGDPLVSSI 128 P I V +N P+A VLKTR D+PDV+ + + + +G+FED + + + Sbjct: 61 PKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEWAKAGVFEDLSNKDYLKRV 120 Query: 129 QPAYVKMLNDLTGSSELNGIPFSANASGVIYNKAMFKEADVAVPTTWDEFIAAAQKFKDS 188 + Y + + ++ +PF+ANA G+ YNK F+E + VP TWDEF + Sbjct: 121 KNGYAE---KYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKVPETWDEFEQLVKDIVAK 177 Query: 189 GKYAFYLTLKDSWTSLTPFNSLEPSIL------GTDFFKKRTEGSVTFSDSFREIAEKQ- 241 G+ F + D+WT L +N L + + + ++ SD + K Sbjct: 178 GQTPFGIAGADAWT-LNGYNQLAFATATGGGKEANQYLRYSQPNAIKLSDPIMKDDIKVM 236 Query: 242 --LKLTEFAQKDIFGKGYNDGNTAFAKGESAMYLQGVWAIPEIMKANPSLELGVFPFPAT 299 L++ QK+ G GY D AFA+G+ M G WAI I + P+ ++G F P Sbjct: 237 DILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAITAINEQKPNFKIGTFMIPG- 295 Query: 300 NDPAKNKVISGVDTLLTISKDSKHKEEAKKFVEFLLQPENVTAYITEQKAFPAVKGVTQ- 358 + ++ + D +IS +KH +EA FVE++ +PE + Y + A++GV Q Sbjct: 296 KEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPEVMQKYYDVDGSPTAIEGVKQA 355 Query: 359 -NDPSTDGFKE-AFTSGNLADFADHY 382 D G E AFT +L ++ Sbjct: 356 GEDSPLAGMTEYAFTDRHLVWLQQYW 381 >lin0762 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 417 Score = 93.6 bits (231), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 102/385 (26%), Positives = 169/385 (43%), Gaps = 79/385 (20%) Query: 5 MKPVTVSLLAMSLL---SACGNNSGSNAATDDGG-------NAASGEKVKIEFFQNKTEA 54 MK V +++L+M LL +ACG GS+++ D G + SG+++K Sbjct: 1 MKKVLLAILSMVLLLSLAACG--GGSDSSKDKDGKEEIVMWGSWSGDQIK---------- 48 Query: 55 KATFDKLVAQFNAANPDIVVSQINPPDAETVLKTRAAKQDLPDVIGLGATDTYKT--LSA 112 +K + +N + V + + E L T A +LPD+I D Y+T + Sbjct: 49 --QLEKQIDSYNKSQDKYKVKYVMQENVEEKLLTGIAGGELPDII---MWDRYQTALYAP 103 Query: 113 SGLFEDFTGDPLVSSIQPAYVKM-------LNDLTGSSELNGIPFSANASGVIYNKAMFK 165 G+ E PL ++ VKM + ++T S +L G+P + + YNK + + Sbjct: 104 KGVLE-----PLDKLVKEDNVKMDDFYEESVKEMTYSDKLYGLPLLNDNRILFYNKKLLQ 158 Query: 166 EADVAVPTTWDEFIAAAQK---------------FKDSGKYAFYLTLKDSWTSLTPFNSL 210 EA V PTTWDE AAQK +D G + YL ++ +TP N Sbjct: 159 EAGVKPPTTWDELATAAQKTTKWDGNKMTQAGMSLQDVGLFNLYL-MQAGGELVTPDNK- 216 Query: 211 EPSILGTDFFKKRTEGSVTFSDSFREIAEKQLKLTEFAQKDIFGKGYNDGNTAFAKGESA 270 T F ++ + + D + LK ++ +G++DG+ AFA G+ A Sbjct: 217 -----KTAFNSEQGLEVLNYWDKM----QNDLK--------VYQRGFDDGSDAFAAGKEA 259 Query: 271 MYLQGVWAIPEIMKANPSLELGVFPFPATNDPAKNKVISGVDTLLTISKDSKHKEEAKKF 330 M G WA+ + K +L+ GV P + K ++ G L + K ++HK+ A F Sbjct: 260 MTYNGPWALADYNKVE-NLDYGVVEPPKGPNGDKGAIMGGFG--LVMPKQAEHKDGAWDF 316 Query: 331 VEFL-LQPENVTAYITEQKAFPAVK 354 +++ +PEN + PA K Sbjct: 317 MKWWTTKPENGVEFAKISGWLPANK 341 >BS_yurO [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 422 Score = 84.3 bits (207), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 92/388 (23%), Positives = 168/388 (43%), Gaps = 44/388 (11%) Query: 1 MKKTMKPVTVSLLAMSLLSACGNNSGSNAATDDGGNAASGEKVKIEFFQN--KTEAKATF 58 MKK + + ++ ++M ++ C + S S DG KV ++FF K K+ F Sbjct: 1 MKKMLLFLIIAAVSMLTIAGCSSQSSSA----DG-------KVTLKFFHRWPKEPEKSYF 49 Query: 59 DKLVAQFNAANPDIVVSQINPPDAETV---LKTRAAKQDLPDVIGLGATDTY-------- 107 +++V +F +PDI + QI ++ +K PD I +D + Sbjct: 50 EEVVKEFEKDHPDIDI-QIEAVLNDSYKDKIKVMLGTTSPPD-IYFSWSDEFAFKFIRGN 107 Query: 108 KTLSASGLFE---DFTGDPLVSSIQPAYVKMLNDLTGSSELNGIPFSANASGVIYNKAMF 164 K L S ++ D++ + S I P T ++ G+P+ +A YNK +F Sbjct: 108 KALDLSSYYKNDTDWSSQLVQSQITP--------FTYENKQYGVPWQMDAKSFFYNKDIF 159 Query: 165 KEADVAVPTTWDEFIAAAQKFKDSGKYAFYLTLKDSWTSLTPFNSLEPSILGTDFFKKRT 224 ++ ++ P TWDE I ++K K+ G K +WT +L ++ +K Sbjct: 160 QKLNLDPPKTWDELIDVSKKLKEHGYTPISFGTKATWTISHYIGTLNQRMVDEKTREKDY 219 Query: 225 EGSV-TFSDS-FREIAEKQLKLTEFAQKDIFGKGYNDGNTAFAKGESAMYLQGVWAIPEI 282 F+D + + EK +L + K + + F G+SAM ++A Sbjct: 220 NAKTGEFTDEGYVKALEKLQELMPYFNKHVNSVDHEYVRQQFKSGKSAM----IYAETAE 275 Query: 283 MKANPSLELGVFPFPA-TNDPAKNKVISGVDTLLTISKDSKHKEEAKKFVEFLLQPENVT 341 +K + LG+FPFP + ++ ++G IS +KH +EA +F++FL Sbjct: 276 IKLVEPVNLGMFPFPEISGQKGSSEALTGAPEGFMISSRTKHPKEAMEFLQFLTSKRMGE 335 Query: 342 AYITEQKAFPAVKGVTQNDPSTDGFKEA 369 + + + AV+G + +T +EA Sbjct: 336 KLVKDVGKYSAVQGTATEENATAIQREA 363 >PH1214 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 441 Score = 76.6 bits (187), Expect = 7e-14, Method: Compositional matrix adjust. Identities = 85/361 (23%), Positives = 155/361 (42%), Gaps = 34/361 (9%) Query: 10 VSLLAMSLLSACGNNSGSNAATDDGGNAASGEKVKIEFFQNKTEAKATFDKLVAQFNAAN 69 V L +S++ A G GS T ++++I + K FD L +F Sbjct: 12 VGALVISVV-ASGCIGGSQTPTSSPTKTQQVQELEIYHWWTAGGEKEAFDALAKKFEKKY 70 Query: 70 PDIVVSQINPPDAETVLKTRAAKQDL------PDVIGLGA-TDTYKTLSASGLFEDFTGD 122 P+I + + NP + + Q L PD + A + + + A+ L D Sbjct: 71 PNIKI-KANPVSGGGGVNLKMVLQSLMLAGKPPDTFQVHAGYEMARYIKANQLTP--IDD 127 Query: 123 PLVSSIQPAYVKMLNDLTG-SSELNGIPFSANASGVI-YNKAMFKE--ADVAVPTTWDEF 178 ++ Y K++ + ++ +P + + + VI YNK +FK+ D + T D+ Sbjct: 128 IWTEDMKKNYPKVIQQMVIFNNHYYAVPINVHRANVIWYNKQIFKKYGIDPSSIKTIDDL 187 Query: 179 IAAAQKFKDSGKYAFYLTLKDSWTSLTPFNSLEPSILGTDFFKKRTEGSVTFSD-SFREI 237 A A+K K++G F L ++ W + F + ++ G D+++K G ++ +D + +++ Sbjct: 188 FAVAEKLKENGVTPFALGDRNKWPATQVFEVMLVAVGGVDYYEKFINGEISANDPTLKKV 247 Query: 238 AEKQLKLTEFAQKDIFGKGYNDGNTAFAKGESAMYLQGVWAIPEIMKA---NPSLELGVF 294 E +K E++ D K +++ KGE+AM L G WA KA P+++ G Sbjct: 248 LEYFVKYVEYSNPDHAAKTWDEACALVYKGEAAMTLMGDWA-NGYFKAKGWKPNVDYGAI 306 Query: 295 PFPATNDPAKNKVISGVDTLLTISKDSKHKEEAKKFVEFLLQPENVTAYITEQKAFPAVK 354 P PA V V + + H AKK++ F+ + Q F +K Sbjct: 307 PIPAG-------VYDLVIDTFVLPAKAAHPNAAKKWLSFI-------GTVEAQNTFNPIK 352 Query: 355 G 355 G Sbjct: 353 G 353 >ycjN [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 430 Score = 74.3 bits (181), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 92/368 (25%), Positives = 157/368 (42%), Gaps = 54/368 (14%) Query: 11 SLLAMSLLSACGNNSGSNAATDDGGNAASGEKVKIEFFQN--KTEAKATFDKLVAQFNAA 68 +L++ +L+S C + +N V IEF + + E +A KL+A+F Sbjct: 11 ALVSCALISGCKEENKTN--------------VSIEFMHSSVEQERQAVISKLIARFEKE 56 Query: 69 NPDIVVSQINPPDAE---TVLKTRAAKQDLPDVIGLGATDTYKTLSASGLFEDFTGDPLV 125 NP I V Q+ P + + T + T + LP+VI + D K + L + ++ Sbjct: 57 NPGITVKQV-PVEEDAYNTKVITLSRSGSLPEVIET-SHDYAKVMDKEQLIDRKAVATVI 114 Query: 126 SSIQPA-----YVKMLNDLTGSSELNGIPFSANASGVIYNKAMFKEADVAVPTTWDEFIA 180 S++ ++++ GS+ G+P SA G+ Y K + +A + P W + + Sbjct: 115 SNVGEGAFYDGVLRIVRTEDGSA-WTGVPVSAWIGGIWYRKDVLAKAGLEEPKNWQQLLD 173 Query: 181 AAQKFKDSG--KYAFYLTLKDSWTSLTPFNSLEPSILGTDFFKKRTEGSVTFSDSFREIA 238 AQK D KY L +S + F+ S F EG +T + Sbjct: 174 VAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVF---NAEGKITL-----DTP 225 Query: 239 EKQLKLTEFAQKDIFGK---GYND---GNTAFAKGESAMYLQGVWAIPEIMKANPSLELG 292 E LT + +D+ G ND AF G + M + + +P ++K +G Sbjct: 226 EMMQALTYY--RDLTANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKEGDPKNVG 283 Query: 293 VFPFPATNDPAKNKVISGVDTLLTIS--KDSKHKEEAKKFVEFLLQPENVTAYI--TEQK 348 F P KN + G+ T LTI+ + ++ E A+KFV F+ Q +N+ ++ + Sbjct: 284 -FVVPT----EKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMMSPGA 338 Query: 349 AFPAVKGV 356 A P K V Sbjct: 339 ALPVNKAV 346 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.313 0.130 0.368 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 30,335,764 Number of extensions: 1276761 Number of successful extensions: 2152 Number of sequences better than 1.0e-05: 41 Number of HSP's gapped: 2126 Number of HSP's successfully gapped: 41 Length of query: 420 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 311 Effective length of database: 25,807,025 Effective search space: 8025984775 Effective search space used: 8025984775 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 117 (49.7 bits)