BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0397.0005.p (270 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin0414 [E] COG0345 Pyrroline-5-carboxylate reductase 138 1e-32 BS_yqjO [E] COG0345 Pyrroline-5-carboxylate reductase 131 1e-30 BS_proH [E] COG0345 Pyrroline-5-carboxylate reductase 124 2e-28 aq_1664 [I] COG0439 Biotin carboxylase 112 4e-25 aq_1663 [N] COG1344 Flagellin and related hook-associated proteins 112 4e-25 >lin0414 [E] COG0345 Pyrroline-5-carboxylate reductase Length = 346 Score = 138 bits (347), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 15/266 (5%), Positives = 38/266 (14%), Gaps = 5/266 (1%) Query: 4 KRIHFIGGGQMAEAIIRAVIGKGAYKAEQISVADVNEERNLYLKNTYGIHAAVSQAAELG 63 + + + G +E + A ++ + Sbjct: 2 QYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPET 61 Query: 64 E-AELIIIAVRPQDDLAAVGRLIAEHAAGQAAVLSIVAGVSIARLGGYAGAERPIIRVIP 122 + + + +LI + + L E + Sbjct: 62 QGLTETYVGNWLAK-HGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 123 NTLTDTGLGYSGAALNGFA---DKAQVDDFLNAFGKVLYLEEAQIDIFTGYGVAGPNYVY 179 L +L +A + + Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 180 YFIESLADAGVLAGLPREQAWQVALENVEGAVAMLRHSGKHPRQLLDINNSPGGVGIHAL 239 + LA Q + Sbjct: 181 ETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGT 240 Query: 240 YELNNSDFAAGLQRSVKAAVKRTTEL 265 + A + T Sbjct: 241 LTGKYLNGAKPAGARNTLFSRFTRYS 266 >BS_yqjO [E] COG0345 Pyrroline-5-carboxylate reductase Length = 346 Score = 131 bits (329), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 19/268 (7%), Positives = 43/268 (16%), Gaps = 6/268 (2%) Query: 4 KRIHFIGGGQMAEAIIRAVIGKGAYKAEQISVAD-VNEERNLYLKNTYGIHAAVSQAAEL 62 + + + G +E + A V E Sbjct: 2 QYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPET 61 Query: 63 GEAELIIIAVRPQDDLAAVGRLIAEHAAGQAAVLSIVAGVSIARLGGYAGAERPIIRVIP 122 + + I + G+ + + + Sbjct: 62 QGLTETYVGNWLAKHGSREKL-IIASKVSGP-SRNNDKGIRPDQALDRKNIREALHDSLK 119 Query: 123 NT---LTDTGLGYSGAALNGFADKAQVDDFLNAFGKVLYLEEAQIDIFTGYGVAGPNYVY 179 D + K +A L + + G V Sbjct: 120 RLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVS 179 Query: 180 YFIESLADAGVLAGLPREQAWQVALENVEGAVAMLRHSGKHPRQLLDINNSPGGVGIHAL 239 + + V ++N + G + + Sbjct: 180 NETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFG 239 Query: 240 YELNNSDFAAGLQRSVKAAVKRTTELGQ 267 A + R T Sbjct: 240 TLTGKYLNGAKPAGARNTLFSRFTRYSG 267 >BS_proH [E] COG0345 Pyrroline-5-carboxylate reductase Length = 346 Score = 124 bits (310), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 24/246 (9%), Positives = 45/246 (18%), Gaps = 5/246 (2%) Query: 4 KRIHFIGGGQMAEAIIRAVIGKGAYKAEQISVAD-VNEERNLYLKNTYGIHAAVSQAAEL 62 G Q +EA A + + + + + A+ Sbjct: 17 GLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKH 76 Query: 63 GEAELIIIAVRPQDDLAAVGRLIAEHAAGQAAVLSIVAGVSIARLGGYAGAERPIIRVIP 122 G E +IIA + + I A A + + + Sbjct: 77 GSREKLIIASKVSGPSRNNDKGIRPDQALDR-KNIREALHDSLKRLQTDYLDLYQVHWPQ 135 Query: 123 NTLTDTGLGYSGAALNGFAD---KAQVDDFLNAFGKVLYLEEAQIDIFTGYGVAGPNYVY 179 G + A + + G Sbjct: 136 RPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADK 195 Query: 180 YFIESLADAGVLAGLPREQAWQVALENVEGAVAMLRHSGKHPRQLLDINNSPGGVGIHAL 239 + + + L E + L L G A Sbjct: 196 HDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGAR 255 Query: 240 YELNNS 245 L + Sbjct: 256 NTLFSR 261 >aq_1664 [I] COG0439 Biotin carboxylase Length = 265 Score = 112 bits (281), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 79/264 (29%), Positives = 135/264 (51%), Gaps = 16/264 (6%) Query: 5 RIHFIGGGQMAEAIIRAVIGKGAYKAEQISVAD-VNEERNLYLKNTYGIHAAVSQAAELG 63 R+ +G G M +A A+ E I V D V E+RNL + + V A+ Sbjct: 9 RVGIVGFGNMGQAF--ALCFSKKLGKENIIVTDKVQEKRNLATEMGIAFASDVKFLAD-- 64 Query: 64 EAELIIIAVRPQDDLAAVGRLIAEHAAGQAAVLSIVAGVSIARLGGYAGAERPIIRVIPN 123 ++++++AV+P+D + +L + +LSI+AGVSI ++ G ++ I+RV+PN Sbjct: 65 NSDVVLVAVKPKDSQEVLQKL----KDYKGIILSIMAGVSIEKMEKILGKDKKIVRVMPN 120 Query: 124 TLTDTGLGYSGAALNGF---ADKAQVDDFLNAFGKVLYLEEAQIDIFTGYGVAGPNYVYY 180 G G NG ++++V++ L + G + +EE D FT +GP +V+ Sbjct: 121 VNVAVGSGVMAITDNGNLSEEERSKVEELLLSCGTLYRIEERLFDAFTALAGSGPAFVFS 180 Query: 181 FIESLADAGVLAGLPREQAWQVALENVEGAVAMLRHSGKHPRQLLDINNSPGGVGIHALY 240 FI++LA AGV G EQA ++AL+ V G+ +L+ +P +L+ SPGG I + Sbjct: 181 FIDALALAGVHQGFSYEQALRIALDTVMGSAKLLKEFQVNPNELIAKVTSPGGTTIEGIK 240 Query: 241 ELNNSDFAAGLQRSVKAAVKRTTE 264 L G + +V + RT++ Sbjct: 241 YLEEK----GFKGTVMECINRTSQ 260 >aq_1663 [N] COG1344 Flagellin and related hook-associated proteins Length = 265 Score = 112 bits (281), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 79/264 (29%), Positives = 135/264 (51%), Gaps = 16/264 (6%) Query: 5 RIHFIGGGQMAEAIIRAVIGKGAYKAEQISVAD-VNEERNLYLKNTYGIHAAVSQAAELG 63 R+ +G G M +A A+ E I V D V E+RNL + + V A+ Sbjct: 9 RVGIVGFGNMGQAF--ALCFSKKLGKENIIVTDKVQEKRNLATEMGIAFASDVKFLAD-- 64 Query: 64 EAELIIIAVRPQDDLAAVGRLIAEHAAGQAAVLSIVAGVSIARLGGYAGAERPIIRVIPN 123 ++++++AV+P+D + +L + +LSI+AGVSI ++ G ++ I+RV+PN Sbjct: 65 NSDVVLVAVKPKDSQEVLQKL----KDYKGIILSIMAGVSIEKMEKILGKDKKIVRVMPN 120 Query: 124 TLTDTGLGYSGAALNGF---ADKAQVDDFLNAFGKVLYLEEAQIDIFTGYGVAGPNYVYY 180 G G NG ++++V++ L + G + +EE D FT +GP +V+ Sbjct: 121 VNVAVGSGVMAITDNGNLSEEERSKVEELLLSCGTLYRIEERLFDAFTALAGSGPAFVFS 180 Query: 181 FIESLADAGVLAGLPREQAWQVALENVEGAVAMLRHSGKHPRQLLDINNSPGGVGIHALY 240 FI++LA AGV G EQA ++AL+ V G+ +L+ +P +L+ SPGG I + Sbjct: 181 FIDALALAGVHQGFSYEQALRIALDTVMGSAKLLKEFQVNPNELIAKVTSPGGTTIEGIK 240 Query: 241 ELNNSDFAAGLQRSVKAAVKRTTE 264 L G + +V + RT++ Sbjct: 241 YLEEK----GFKGTVMECINRTSQ 260 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,342,766 Number of extensions: 857745 Number of successful extensions: 2189 Number of sequences better than 1.0e-05: 15 Number of HSP's gapped: 2170 Number of HSP's successfully gapped: 15 Length of query: 270 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 165 Effective length of database: 26,268,361 Effective search space: 4334279565 Effective search space used: 4334279565 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)