BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0397.0003.p (258 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yqiK [C] COG0584 Glycerophosphoryl diester phosphodiesterase 202 3e-52 CAC0430 [C] COG0584 Glycerophosphoryl diester phosphodiesterase 185 6e-47 BH3700 [C] COG0584 Glycerophosphoryl diester phosphodiesterase 183 2e-46 FN1891 [C] COG0584 Glycerophosphoryl diester phosphodiesterase 164 9e-41 BH1080 [C] COG0584 Glycerophosphoryl diester phosphodiesterase 164 1e-40 >BS_yqiK [C] COG0584 Glycerophosphoryl diester phosphodiesterase Length = 553 Score = 202 bits (515), Expect = 3e-52, Method: Compositional matrix adjust. Identities = 12/232 (5%), Positives = 41/232 (17%), Gaps = 2/232 (0%) Query: 20 FAHRGASAVCPENTMAAFRKGLELGATGIETDVQMTLDGRLILIHDESLNRTTNRSGLVK 79 + + + A R L + + + Sbjct: 5 VNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVRTGLGGQKVVMSGGAIVMPIF 64 Query: 80 DMTLSEVLKADAGSWFGAEFAGERIPVLEELLDLLQGRDTILNIELKNGTYLYPGMEEKV 139 + + + + ++ + T + + Sbjct: 65 HEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFFVRVKPSVEGIATAAQTLGQRTL 124 Query: 140 IAAVREYGMSERIVLSSFNHYSLAYCKSLAPEIRTGILYGEGLYRPWDYAASLQADALHA 199 + ++ V + + L + + L+ +++ Sbjct: 125 SPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENFVQGVQNTVAEDLSKNGLELESVSL 184 Query: 200 Y--HLAVLPEFVAGAAEHGVVYHPWTVNDPERMKYLIEAGVAGIITDVPDVL 249 + F A T R + E + Sbjct: 185 TNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRRERNEVEQDVEVAVREKNR 236 >CAC0430 [C] COG0584 Glycerophosphoryl diester phosphodiesterase Length = 249 Score = 185 bits (469), Expect = 6e-47, Method: Compositional matrix adjust. Identities = 102/238 (42%), Positives = 148/238 (62%), Gaps = 1/238 (0%) Query: 18 INFAHRGASAVCPENTMAAFRKGLELGATGIETDVQMTLDGRLILIHDESLNRTTNRSGL 77 +N AHRG S PENTM AF+K +E GIETD+ MT DG L++ HDE ++RTTN G Sbjct: 6 LNIAHRGFSGKYPENTMVAFKKAIEEKCDGIETDLNMTKDGILVVCHDEKIDRTTNGCGY 65 Query: 78 VKDMTLSEVLKADAGSWFGAEFAGERIPVLEELLDLLQGRDTILNIELKNGTYLYPGMEE 137 +KD T E+ + DAG +G EF GE+I ++ELLD ++ ++ +LN+ELKN Y +E+ Sbjct: 66 IKDYTYKELKQFDAGIGYGEEFKGEKILCIDELLDYIKDKNLLLNLELKNNLIEYVDIEK 125 Query: 138 KVIAAVREYGMSERIVLSSFNHYSLAYCKSLAPEIRTGILYGEGLYRPWDYAASLQADAL 197 KV+ + EY + + I++SSFNHYS+ K ++ G+LY LY +Y L A +L Sbjct: 126 KVVDKIHEYKLEDNIIVSSFNHYSMVKLKEYDSTMKIGLLYSANLYNVHEYGRKLGAFSL 185 Query: 198 HAYHLAVL-PEFVAGAAEHGVVYHPWTVNDPERMKYLIEAGVAGIITDVPDVLAGLLA 254 H Y+ AV+ E V + + + +TVN+ E M+ LI G+ GIIT+ PD L +L Sbjct: 186 HPYYPAVMNKEVVENIKKVKININTYTVNEEEDMRKLIGLGIEGIITNYPDRLNKVLK 243 >BH3700 [C] COG0584 Glycerophosphoryl diester phosphodiesterase Length = 284 Score = 183 bits (465), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 104/237 (43%), Positives = 138/237 (58%), Gaps = 9/237 (3%) Query: 18 INFAHRGASAVCPENTMAAFRKGLELGATGIETDVQMTLDGRLILIHDESLNRTTNRSGL 77 IN AHRGAS PENTMAAF+KG ++ IE DV MT DG L+L+HD +++RTTN G Sbjct: 40 INIAHRGASGHAPENTMAAFQKGFDMKVDYIEFDVHMTKDGELVLMHDLTVDRTTNGHGY 99 Query: 78 VKDMTLSEVLKADAGSWFGAEFAGERIPVLEELLDLLQGRDTILNIELKNGTYLYPGMEE 137 V D+TL E+ + DAGSWF +F GE++P EE LD+ +G+ +L IELK+ YPGMEE Sbjct: 100 VSDLTLDEIKQFDAGSWFHEDFVGEKVPTFEETLDVFRGKVGLL-IELKSPER-YPGMEE 157 Query: 138 KVIAAVREYGM----SERIVLSSFNHYSLAYCKSLAPEIRTGILYGEGLYRPWDYAA--- 190 KV A++E M + ++++ SFNH S+ K L P I G+L G D Sbjct: 158 KVAEALKERKMDRSSNHKVIIQSFNHESMKKSKELLPHIPHGVLAGPSWANVTDEQLEEF 217 Query: 191 SLQADALHAYHLAVLPEFVAGAAEHGVVYHPWTVNDPERMKYLIEAGVAGIITDVPD 247 S AD + V PE V+ G+ P+T E+ L E V GIITD P+ Sbjct: 218 STYADYFNPNMTIVTPELVSRIHAVGMKIFPYTSRTQEQANRLFELKVDGIITDFPE 274 >FN1891 [C] COG0584 Glycerophosphoryl diester phosphodiesterase Length = 261 Score = 164 bits (416), Expect = 9e-41, Method: Compositional matrix adjust. Identities = 85/237 (35%), Positives = 139/237 (58%), Gaps = 4/237 (1%) Query: 20 FAHRGASAVCPENTMAAFRKGLELGATGIETDVQMTLDGRLILIHDESLNRTTNRSGLVK 79 FAHRGAS PENT+ A +K +E+ A GIE D+Q+T DG++++IHD ++RTT G V Sbjct: 25 FAHRGASGYAPENTLTAIKKAIEMKADGIEIDIQLTKDGKIVVIHDWKVDRTTTGRGFVY 84 Query: 80 DMTLSEVLKADAGSWFGAEFAGERIPVLEELLDLLQGRDTILNIELKNGTYLYPGMEEKV 139 ++ + DAG W+ +F GE +P LEE+LD+L D +LNIE+K+ + +EEK+ Sbjct: 85 ELDFDYIRSLDAGQWYTKDFVGEVVPTLEEVLDVLPN-DMMLNIEIKDTARKHSNIEEKM 143 Query: 140 IAAVREYGMS-ERIVLSSFNHYSLAYCKSLAPEIRTGILYGEGLYRPWDYAAS--LQADA 196 + +++Y E I++SSF+H + + L P+++ +L Y ++ L++ + Sbjct: 144 LEVLKKYPEKFEDIIVSSFHHDKIKRLQELEPKLKLALLTDSEFIEIEKYLSTNGLKSYS 203 Query: 197 LHAYHLAVLPEFVAGAAEHGVVYHPWTVNDPERMKYLIEAGVAGIITDVPDVLAGLL 253 H + + V ++ V WTVN E + YL+ GV G+IT+ PD++ LL Sbjct: 204 YHPEINLISKKDVEILHKNDVKVFVWTVNKEEDLDYLVTLGVDGVITNYPDIMKELL 260 >BH1080 [C] COG0584 Glycerophosphoryl diester phosphodiesterase Length = 250 Score = 164 bits (415), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 88/229 (38%), Positives = 128/229 (55%) Query: 20 FAHRGASAVCPENTMAAFRKGLELGATGIETDVQMTLDGRLILIHDESLNRTTNRSGLVK 79 +AHRG S PENT++AFR +GA GIE DVQ+T D +LI+IHD LNRTT+ SGLV+ Sbjct: 10 YAHRGVSCKYPENTLSAFRAAEGVGADGIELDVQLTKDDQLIVIHDLHLNRTTSGSGLVR 69 Query: 80 DMTLSEVLKADAGSWFGAEFAGERIPVLEELLDLLQGRDTILNIELKNGTYLYPGMEEKV 139 TLSE+ + DAGSWF F GE +P L+E+ Q + I N+ELK + + Sbjct: 70 AHTLSELKRLDAGSWFDERFTGETLPTLDEVFAWAQSNELIFNVELKGVVEDRDRLFTAI 129 Query: 140 IAAVREYGMSERIVLSSFNHYSLAYCKSLAPEIRTGILYGEGLYRPWDYAASLQADALHA 199 A + + M +R++LSSF+H + + ++ LY P Y + A H Sbjct: 130 SACIDTFKMRDRVILSSFDHVLIKKLSGSFTRNQLAVIVMAALYDPGGYVQKVGATGYHF 189 Query: 200 YHLAVLPEFVAGAAEHGVVYHPWTVNDPERMKYLIEAGVAGIITDVPDV 248 Y+LA+ V G+ P+T+N E ++ ++ GV GI TD P++ Sbjct: 190 YYLAMTEAEVYHLLHKGLKLRPFTLNQEEDIRRYMQLGVNGIFTDDPEL 238 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.137 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,262,639 Number of extensions: 763966 Number of successful extensions: 1606 Number of sequences better than 1.0e-05: 73 Number of HSP's gapped: 1536 Number of HSP's successfully gapped: 73 Length of query: 258 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 154 Effective length of database: 26,383,695 Effective search space: 4063089030 Effective search space used: 4063089030 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)