BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0396.0003.p (393 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value YPO3394 [L] COG1643 HrpA-like helicases 270 3e-72 PA3961 [L] COG1643 HrpA-like helicases 259 5e-69 ZhrpB [L] COG1643 HrpA-like helicases 254 2e-67 ECs0152 [L] COG1643 HrpA-like helicases 254 2e-67 hrpB [L] COG1643 HrpA-like helicases 252 6e-67 >YPO3394 [L] COG1643 HrpA-like helicases Length = 828 Score = 270 bits (690), Expect = 3e-72, Method: Compositional matrix adjust. Identities = 151/343 (44%), Positives = 218/343 (63%), Gaps = 7/343 (2%) Query: 53 QRESRSFLAQLTAAPGALPDNISLTGLLLSFAYPDRIGQKRG-DGAFLLSGGRGAAMREG 111 QR +R +L A G++ + +L LL+ AYPDRI Q+RG DG +LL+ G GAAM + Sbjct: 483 QRRARQLTQRLNARGGSV--DHALAAQLLALAYPDRIAQQRGQDGRYLLANGMGAAMAQD 540 Query: 112 QPLTRSPYIVAAGI--DDRAGQSRIMLAADLPEADLLKYHKDSLQEEREVYWDKESGSVQ 169 + L+RSP++VA G+ + +RI+LA + L+ ++E V WD E G+++ Sbjct: 541 EALSRSPWLVAVGLLQNHSGPDARILLALPVDIEQLVTCLPRQVEERTVVEWDDEKGTLR 600 Query: 170 ARRRTLLGALILKETSHERPTAKETEEVLLRVIAEEGLKLLPWDKATVQLRQRMEFLHGL 229 A RR +G LIL+ +P ++ LL + E+GL++L WD+ QLR R++ Sbjct: 601 AWRRWQIGRLILRAQPLAKPDDAALQQALLNWVREQGLQILNWDEPAEQLRTRLQCAARW 660 Query: 230 RPD--FPDVSEEALLSTLQEWLLPFIQGMRNLRDLQRVPVSRALEGLLDWNSRQLLDREA 287 P+ +P V +++L L WL P + G+R+LR L++V ++ AL LLDW RQ LD E Sbjct: 661 LPEAPWPAVDDDSLQDKLALWLQPSLSGVRDLRGLRQVNIAEALTRLLDWKQRQRLDTEL 720 Query: 288 PAHITVPSGSRIPVDYSSPSAPVLAVRLQEMFGQADTPRIGGGKVAVLLHLLSPARRPVQ 347 P H TVP+GSR+P+ Y PVLAVRLQE+FG+ +P + G+VAV+L LLSPA RP+Q Sbjct: 721 PTHYTVPTGSRLPIRYYVDRPPVLAVRLQEVFGEQQSPMLASGRVAVVLELLSPAHRPLQ 780 Query: 348 VTADLASFWRSTYFEVKKDLKGRYPKHYWPDDPLQAAPTNRTR 390 +T DLA+FW+ Y EV+K++KGRYPKH WPDDP PT RT+ Sbjct: 781 ITGDLAAFWQGAYREVQKEMKGRYPKHVWPDDPAHTRPTRRTK 823 >PA3961 [L] COG1643 HrpA-like helicases Length = 838 Score = 259 bits (662), Expect = 5e-69, Method: Compositional matrix adjust. Identities = 168/400 (42%), Positives = 228/400 (57%), Gaps = 16/400 (4%) Query: 4 LAALLQERDLLQGPAALDSDLTLRVEALLRFERXXXXXXXXXXXXLRAVQRESRSFLAQL 63 +AALL ERD+ +G A DL R+ ALL E R + R+ R +L Sbjct: 445 VAALLGERDIQRGGGA---DLHSRL-ALLAGE-ARTGASRGAVQRARQLARQFRGYLRGA 499 Query: 64 TAAPGALPDNISLTGLLLSFAYPDRIGQKR--GDGAFLLSGGRGAAMREGQPLTRSPYIV 121 + P + G LL+FAYPDRI ++R G G + L+ GR A E L + P++V Sbjct: 500 ASEAVVDPGHPRWLGCLLAFAYPDRIARQRRAGGGDYRLANGRAAQFGEPDSLMKQPWLV 559 Query: 122 AAGIDDRAGQ--SRIMLAADLPEADLLKYHKDSLQEEREVYWDKESGSVQARRRTLLGAL 179 A + R GQ RI LAA+L + + + E+ WD+ G ++A R+ +G L Sbjct: 560 IADLGSRQGQREERIYLAAELDPRLFDTVLAEQVSQRDELQWDEREGVLRAERQRRVGEL 619 Query: 180 ILKETSHERPTAKETEEVLLRVIAEEGLKLLPWD------KATVQLRQRMEFLHGLRPDF 233 +L + + LL ++ +GL+LLPW +A + L +R++ ++ Sbjct: 620 VLSSEALPGLDEAARSQALLGLVRRKGLELLPWTPELRQWQARIGLLRRLDLEDKGESEW 679 Query: 234 PDVSEEALLSTLQEWLLPFIQGMRNLRDLQRVPVSRALEGLLDWNSRQLLDREAPAHITV 293 PDVS+ ALL L+EWL ++ + L + ++ L GLL W Q LD AP + V Sbjct: 680 PDVSDAALLERLEEWLPAYLGKVTRLAHFANLDLASILAGLLPWPLPQRLDEWAPKTLEV 739 Query: 294 PSGSRIPVDYSSPSAPVLAVRLQEMFGQADTPRIGGGKVAVLLHLLSPARRPVQVTADLA 353 PSGSRI +DYS + P+LAVRLQE+FG DTPRI G++AV LHLLSPA RPVQVT DLA Sbjct: 740 PSGSRIRLDYSE-TPPILAVRLQELFGLGDTPRIAQGRLAVKLHLLSPAHRPVQVTQDLA 798 Query: 354 SFWRSTYFEVKKDLKGRYPKHYWPDDPLQAAPTNRTRPAK 393 +FWRSTY EV+KDLKGRYPKHYWPDDPL A T R +P K Sbjct: 799 NFWRSTYAEVRKDLKGRYPKHYWPDDPLVAEATARAKPRK 838 >ZhrpB [L] COG1643 HrpA-like helicases Length = 824 Score = 254 bits (649), Expect = 2e-67, Method: Compositional matrix adjust. Identities = 142/345 (41%), Positives = 210/345 (60%), Gaps = 7/345 (2%) Query: 51 AVQRESRSFLAQLTAAPGALPDNISLTGLLLSFAYPDRIGQKRG-DGAFLLSGGRGAAMR 109 A Q+ S+ L +L G + SL LL+ A+ DRI ++RG DG + L+ G GA + Sbjct: 479 AWQQRSQQLLKRLNVRGGEA--DSSLIAPLLAGAFADRIARRRGQDGRYQLANGMGAMLD 536 Query: 110 EGQPLTRSPYIVAAGI--DDRAGQSRIMLAADLPEADLLKYHKDSLQEEREVYWDKESGS 167 L+R +++A + + +RI+LA + +L++ +Q+ V WD G+ Sbjct: 537 ADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQLVQQSDTVEWDDAQGT 596 Query: 168 VQARRRTLLGALILKETSHERPTAKETEEVLLRVIAEEGLKLLPWDKATVQLRQRMEFLH 227 ++A RR +G L +K +P+ E + +L I ++GL +L W QLR R+ Sbjct: 597 LKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVLNWTAEAEQLRLRLLCAG 656 Query: 228 GLRP--DFPDVSEEALLSTLQEWLLPFIQGMRNLRDLQRVPVSRALEGLLDWNSRQLLDR 285 P D+P V +E+LL+TL+ WLLP + G+ +LR L+ + + +AL GLLDW +Q LD Sbjct: 657 KWLPEYDWPAVDDESLLATLETWLLPHMAGVHSLRGLKSLDIYQALRGLLDWGMQQRLDS 716 Query: 286 EAPAHITVPSGSRIPVDYSSPSAPVLAVRLQEMFGQADTPRIGGGKVAVLLHLLSPARRP 345 E PAH TVP+GSRI + Y + P LAVR+QEMFG+A P I G+V ++L LLSPA+RP Sbjct: 717 ELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATNPTIAQGRVPLVLELLSPAQRP 776 Query: 346 VQVTADLASFWRSTYFEVKKDLKGRYPKHYWPDDPLQAAPTNRTR 390 +Q+T DL +FW+ Y EV+K++KGRYPKH WPDDP APT RT+ Sbjct: 777 LQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPANTAPTRRTK 821 >ECs0152 [L] COG1643 HrpA-like helicases Length = 824 Score = 254 bits (649), Expect = 2e-67, Method: Compositional matrix adjust. Identities = 142/345 (41%), Positives = 210/345 (60%), Gaps = 7/345 (2%) Query: 51 AVQRESRSFLAQLTAAPGALPDNISLTGLLLSFAYPDRIGQKRG-DGAFLLSGGRGAAMR 109 A Q+ S+ L +L G + SL LL+ A+ DRI ++RG DG + L+ G GA + Sbjct: 479 AWQQRSQQLLKRLNVRGGEA--DSSLIAPLLAGAFADRIARRRGQDGRYQLANGMGAMLD 536 Query: 110 EGQPLTRSPYIVAAGI--DDRAGQSRIMLAADLPEADLLKYHKDSLQEEREVYWDKESGS 167 L+R +++A + + +RI+LA + +L++ +Q+ V WD G+ Sbjct: 537 ADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQLVQQSDTVEWDDAQGT 596 Query: 168 VQARRRTLLGALILKETSHERPTAKETEEVLLRVIAEEGLKLLPWDKATVQLRQRMEFLH 227 ++A RR +G L +K +P+ E + +L I ++GL +L W QLR R+ Sbjct: 597 LKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVLNWTAEAEQLRLRLLCAG 656 Query: 228 GLRP--DFPDVSEEALLSTLQEWLLPFIQGMRNLRDLQRVPVSRALEGLLDWNSRQLLDR 285 P D+P V +E+LL+TL+ WLLP + G+ +LR L+ + + +AL GLLDW +Q LD Sbjct: 657 KWLPEYDWPAVDDESLLATLETWLLPHMAGVHSLRGLKSLDIYQALRGLLDWGMQQRLDS 716 Query: 286 EAPAHITVPSGSRIPVDYSSPSAPVLAVRLQEMFGQADTPRIGGGKVAVLLHLLSPARRP 345 E PAH TVP+GSRI + Y + P LAVR+QEMFG+A P I G+V ++L LLSPA+RP Sbjct: 717 ELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATNPTIAQGRVPLVLELLSPAQRP 776 Query: 346 VQVTADLASFWRSTYFEVKKDLKGRYPKHYWPDDPLQAAPTNRTR 390 +Q+T DL +FW+ Y EV+K++KGRYPKH WPDDP APT RT+ Sbjct: 777 LQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPANTAPTRRTK 821 >hrpB [L] COG1643 HrpA-like helicases Length = 824 Score = 252 bits (644), Expect = 6e-67, Method: Compositional matrix adjust. Identities = 141/345 (40%), Positives = 209/345 (60%), Gaps = 7/345 (2%) Query: 51 AVQRESRSFLAQLTAAPGALPDNISLTGLLLSFAYPDRIGQKRG-DGAFLLSGGRGAAMR 109 A Q+ S+ L +L G + SL LL+ A+ DRI ++RG DG + L+ G GA + Sbjct: 479 AWQQRSQQLLKRLNVRGGE--ADSSLIAPLLAGAFADRIARRRGQDGRYQLANGMGAMLD 536 Query: 110 EGQPLTRSPYIVAAGI--DDRAGQSRIMLAADLPEADLLKYHKDSLQEEREVYWDKESGS 167 L+R +++A + + +RI+LA + +L++ +Q+ V WD G+ Sbjct: 537 ANDALSRHEWLIAPLLLQGSASPDARILLALLVDIDELVQRCPQLVQQSDTVEWDDAQGT 596 Query: 168 VQARRRTLLGALILKETSHERPTAKETEEVLLRVIAEEGLKLLPWDKATVQLRQRMEFLH 227 ++A RR +G L +K +P+ E + +L I ++GL +L W QLR R+ Sbjct: 597 LKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVLNWTAEAEQLRLRLLCAA 656 Query: 228 GLRP--DFPDVSEEALLSTLQEWLLPFIQGMRNLRDLQRVPVSRALEGLLDWNSRQLLDR 285 P D+P V +E+LL+ L+ WLLP + G+ +LR L+ + + +AL GLLDW +Q LD Sbjct: 657 KWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLDIYQALRGLLDWGMQQRLDS 716 Query: 286 EAPAHITVPSGSRIPVDYSSPSAPVLAVRLQEMFGQADTPRIGGGKVAVLLHLLSPARRP 345 E PAH TVP+GSRI + Y + P LAVR+QEMFG+A P I G+V ++L LLSPA+RP Sbjct: 717 ELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATNPTIAQGRVPLVLELLSPAQRP 776 Query: 346 VQVTADLASFWRSTYFEVKKDLKGRYPKHYWPDDPLQAAPTNRTR 390 +Q+T DL+ FW+ Y EV+K++KGRYPKH WPDDP APT RT+ Sbjct: 777 LQITRDLSDFWKGAYREVQKEMKGRYPKHVWPDDPANTAPTRRTK 821 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,241,785 Number of extensions: 1161738 Number of successful extensions: 1934 Number of sequences better than 1.0e-05: 15 Number of HSP's gapped: 1890 Number of HSP's successfully gapped: 15 Length of query: 393 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 285 Effective length of database: 25,922,359 Effective search space: 7387872315 Effective search space used: 7387872315 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)