BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0394.0002.p (397 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC0451 [T] COG0642 Signal transduction histidine kinase 228 2e-59 lin1543 [T] COG0642 Signal transduction histidine kinase 107 3e-23 lin2643 [T] COG0642 Signal transduction histidine kinase 99 2e-20 BS_resE_4 [T] COG0642 Signal transduction histidine kinase 92 2e-18 all0330_3 [T] COG0642 Signal transduction histidine kinase 89 9e-18 >CAC0451 [T] COG0642 Signal transduction histidine kinase Length = 416 Score = 228 bits (580), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 124/388 (31%), Positives = 207/388 (53%), Gaps = 1/388 (0%) Query: 1 MLALLMFFFMNYQVSQINKAVVNQNAALIGYVLKQAPQLENEIIHFITQGAQEDEIVAGK 60 + +L+++ F++ N ++ N A + +++ P +++E++ T+ ++ + G Sbjct: 23 IFSLVLYIFLDRMAINANNGLIKTNGAAMETLIEAHPDMKDEVVRAFTKKPTKEMLEKGI 82 Query: 61 RSLAQYAYTE-DMLVNDQPILSDNALPFKTAAQVXXXXXXXXXXXGWEYRKIFDKIRAIT 119 QY + + + +N N L T + ++ +F KI + Sbjct: 83 ELSNQYGFDQSEYNLNISLKTFHNQLIILTFSIFILYFLFNVLLISSQFGSVFMKINEVH 142 Query: 120 FAAEQVVEHQFDQPLPENDEGDFGTLGRNFNAMAERLHNSLRQLQQEKTFLRNLLSDISH 179 A++++ + L + EG+ G L +FN M+ + N+L L++EK FL+N++ DISH Sbjct: 143 EGAQKIINGNYSLRLNDYREGELGKLSASFNRMSSIIENTLNNLKEEKIFLKNMMQDISH 202 Query: 180 QLKTPLAALIVFNENLLNDPHMKEEMRTKFLDRSRLQLERMEWLIISLLKLARVEAGAIT 239 QLKTPL++L +FNE LLN E+ +FL S+ QL++MEWLII+LLK+AR++AGAI Sbjct: 203 QLKTPLSSLKIFNELLLNQNIKDEDTMDEFLHESKKQLDKMEWLIINLLKMARLDAGAID 262 Query: 240 FQKERVRVREMIDHAVHSLRMLSEQRKQQIHIHGGEAMYVWADEEWLTEAIINLIKNALE 299 F+ E+ + E + ++ L+ + + Q++ + + Y D++WL EA N+IKN +E Sbjct: 263 FKNEKRSIEETVCESISLLKPMYTLKNQKVSVESTDKAYFKHDKKWLLEAFSNIIKNCIE 322 Query: 300 HTPLEGAIDVILEENSVFQTVIIRDRGEGISSEDLPHIFERFYSGRSTEKPQXXXXXXXX 359 HT G I + + + + V I D G GIS +DLPHIFERFY G+ Sbjct: 323 HTGDNGEIKIHVYRSPIMVKVTIEDNGSGISKDDLPHIFERFYKGKGVYNKSSTGIGLSM 382 Query: 360 XXXXXEEQGGMITVVSQLGIGTEFRISF 387 E QGG ITV S + GT F I F Sbjct: 383 AKSIIENQGGFITVKSAVSAGTIFNIVF 410 >lin1543 [T] COG0642 Signal transduction histidine kinase Length = 182 Score = 107 bits (268), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 0/278 (0%), Positives = 0/278 (0%), Gaps = 9/278 (3%) Query: 113 DKIRAITFAAEQVVEHQFDQPLPENDEGDFGTLGRNFNAMAERLHNSLRQLQQEKTFLRN 172 Sbjct: 196 255 Query: 173 LLSDISHQLKTPLAALIVFNENLLND--PHMKEEMRTKFLDRSRLQLERMEWLIISLLKL 230 Sbjct: 256 --- 312 Query: 231 ARVEAGAITFQKERVRVREMIDHAVHSLRMLSEQRKQQIHIHGGEAMYVWADEEWLTEAI 290 Sbjct: 313 372 Query: 291 INLIKNALEHTPLEGAIDVILEENSVFQTVIIRDRGEGISSEDLPHIFERFYSG---RST 347 Sbjct: 373 - 431 Query: 348 EKPQXXXXXXXXXXXXXEEQGGMITVVSQLGIGTEFRI 385 Sbjct: 432 469 >lin2643 [T] COG0642 Signal transduction histidine kinase Length = 182 Score = 98.6 bits (244), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 0/275 (0%), Positives = 0/275 (0%), Gaps = 16/275 (5%) Query: 120 FAAEQVVEHQFDQPLPENDEGDFGTLGRNFNAMAERLHNSLRQLQQEKTFLRNLLSDISH 179 Sbjct: 326 ---------- 375 Query: 180 QLKTPLAALIVFNENLLNDPHMKEEMRTKFLDRSRLQLERMEWLIISLLKLARVEAGAIT 239 Sbjct: 376 435 Query: 240 FQKERVRVREMIDHAVHSLRMLSEQRKQQIHI--HGGEAMYVWADEEWLTEAIINLIKNA 297 Sbjct: 436 495 Query: 298 LEHTPLEGAIDVILEENSVFQTVIIRDRGEGISSEDLPHIFERFY---SGRSTEKPQXXX 354 Sbjct: 496 - 554 Query: 355 XXXXXXXXXXEEQGGMITVVSQLGIGTEFRISFNK 389 Sbjct: 555 589 >BS_resE_4 [T] COG0642 Signal transduction histidine kinase Length = 269 Score = 91.7 bits (226), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 68/233 (29%), Positives = 113/233 (48%), Gaps = 12/233 (5%) Query: 160 LRQLQQEKTF--LR-NLLSDISHQLKTPLAALIVFNENLLNDPHMKEEMRTKFLDRSRLQ 216 LR + +E+ LR + ++++SH+L+TP++ L ++E +++D EE R + + Sbjct: 32 LRDMTEERRLDKLREDFIANVSHELRTPISMLQGYSEAIVDDIASSEEDRKEIAQIIYDE 91 Query: 217 LERMEWLIISLLKLARVEAGAITFQKERVRVREMIDHAVHSLRMLSEQRK----QQIHIH 272 RM L+ LL LAR+E+G E++ V E ++ + +++++ I + Sbjct: 92 SLRMGRLVNDLLDLARMESGHTGLHYEKINVNEFLEKIIRKFSGVAKEKNIALDHDISLT 151 Query: 273 GGEAMYVWADEEWLTEAIINLIKNALEHTPLEGAIDVILEENSVFQTVIIRDRGEGISSE 332 E M+ DE+ + + NLI NAL HT G++ + + + I+D G GI E Sbjct: 152 EEEFMF---DEDKMEQVFTNLIDNALRHTSAGGSVSISVHSVKDGLKIDIKDSGSGIPEE 208 Query: 333 DLPHIFERFYSGRS--TEKPQXXXXXXXXXXXXXEEQGGMITVVSQLGIGTEF 383 DLP IFERFY T E G ITV S++ GT F Sbjct: 209 DLPFIFERFYKADKARTRGRAGTGLGLAIVKNIVEAHNGSITVHSRIDKGTTF 261 >all0330_3 [T] COG0642 Signal transduction histidine kinase Length = 242 Score = 89.4 bits (220), Expect = 9e-18, Method: Compositional matrix adjust. Identities = 57/175 (32%), Positives = 91/175 (52%), Gaps = 5/175 (2%) Query: 172 NLLSDISHQLKTPLAALIVFNENLLNDPHMKEEMRTKFLDRSRLQLERMEWLIISLLKLA 231 NL++ H+L+TPL+ + V E+L +P M E++ L+ + ERM L+ L L+ Sbjct: 4 NLIAITGHELRTPLSTIQVCLESLATEPDMPVELQQVMLNTALSDSERMRKLVQDFLTLS 63 Query: 232 RVEAGAITFQKERVRVREMIDHAVHSLRMLSEQRKQ---QIHIHGGEAMYVWADEEWLTE 288 +E+G + + E + ++E ID A+ LR+ K + I G + V AD +WL E Sbjct: 64 NLESGRVEWHPESLTLQECIDLALSRLRIRPGVEKPPAIKTQIDGNLPL-VRADGDWLVE 122 Query: 289 AIINLIKNALEHTPLEGAIDVILEEN-SVFQTVIIRDRGEGISSEDLPHIFERFY 342 + L+ NA + TP +G I + N S V + D G GI L +F+RFY Sbjct: 123 VLAKLVDNACKFTPTQGEIKISATHNGSQMVEVTVADTGRGIEPNRLEIVFDRFY 177 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.135 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 25,724,117 Number of extensions: 949955 Number of successful extensions: 2847 Number of sequences better than 1.0e-05: 177 Number of HSP's gapped: 2653 Number of HSP's successfully gapped: 177 Length of query: 397 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 288 Effective length of database: 25,807,025 Effective search space: 7432423200 Effective search space used: 7432423200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)