BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0392.0005.p (542 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value TP0767 [J] COG0480 Translation elongation factors (GTPases) 186 1e-46 TM1503 [J] COG0480 Translation elongation factors (GTPases) 184 3e-46 YLR069c [J] COG0480 Translation elongation factors (GTPases) 184 4e-46 BB0540 [J] COG0480 Translation elongation factors (GTPases) 175 1e-43 FN1556 [J] COG0480 Translation elongation factors (GTPases) 175 2e-43 >TP0767 [J] COG0480 Translation elongation factors (GTPases) Length = 695 Score = 186 bits (471), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 127/466 (27%), Positives = 227/466 (48%), Gaps = 21/466 (4%) Query: 31 RTFAIISHPDAGKTTLTEKLLLFGGAIRLAGSVKARKASKHATSDWMEIEKQRGISVTSS 90 R I +H D+GKTTL+E++L + I V+ K AT D ME+E++RGI++ S+ Sbjct: 9 RNIGISAHIDSGKTTLSERILFYCDRIHAIHEVRG-KDGVGATMDNMELERERGITIQSA 67 Query: 91 VMQFDYMGHRVNILDTPGHQDFSEDTYRTLTAADSAVMLIDVAKGVEAQTIKLFQVCAKR 150 Q + GH +N++DTPGH DF+ + R+L D AV+++ GV++Q+I + + + Sbjct: 68 STQVQWKGHTINVIDTPGHVDFTIEVERSLRVLDGAVLVLCSVAGVQSQSITVDRQLRRY 127 Query: 151 GIPIFTFINKLDREGQSPFELMEELENVLGIRSVPMNWPIGGGRELCGVYDRMKNQVELF 210 +P +FINK DR G +PF++ +L L + + M PIG L GV D + + F Sbjct: 128 HVPRISFINKCDRTGANPFKVCAQLREKLSLNAHLMQLPIGLEDRLEGVIDLISLKALYF 187 Query: 211 QGDDHSVIKVQKVESYNDPVIREMAGEYLHDQLCQDLELLDVAGDAFDYEKVLRG----- 265 +G+ + ++ + +++ E + EL + + + ++++R Sbjct: 188 EGESGAHVREAPIPEQYQADVKKYRDELIDAASLFSDELAEAYLEGTETDQLIRAAVRAG 247 Query: 266 ----ELTPVFFGSAINNFGVQTFLDNFLELAPKPEPRRSNAGDVEPTNEKFT------GY 315 + PVF GSA N G+Q LD P P + A D++ E T Sbjct: 248 TIAEKFVPVFCGSAYKNKGIQPLLDAITYYLPDPTEVTNTALDLDRAEEPVTLSTDADAP 307 Query: 316 VFKIQANMNPAHRDRIAFLRIVSGKFERGMSVKHVRAGKDIKLSQPQQFLAQDRDIVEEA 375 V + + ++ ++R+ G ++G + +VRA K K+ + + + + + E Sbjct: 308 VVALGFKLEDGKYGQLTYVRVYQGTIKKGAELFNVRARKKFKVGRLVRMNSNQMEDISEG 367 Query: 376 FPGDIIGLFDPGIFRIGDTLSQAGDIEFDELPTFSPEIFSKVSI--KNALKSKQFQKGID 433 PGDI+ LF GDT +GD+ + F PE +SI K+ + Q K ++ Sbjct: 368 TPGDIVALFGVDCAS-GDTFC-SGDLNYAMTSMFVPEPVISLSITPKDKRSADQVSKALN 425 Query: 434 QLT-EEGMIQVFRTVNFDDILLGVVGQLQFEVFEYRMKGEYGVDVQ 478 + T E+ + F + ++ +G+L +V+ RM+ EY +V+ Sbjct: 426 RFTKEDPTFRSFVDPESNQTIIQGMGELHLDVYIERMRREYKCEVE 471 >TM1503 [J] COG0480 Translation elongation factors (GTPases) Length = 695 Score = 184 bits (467), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 138/477 (28%), Positives = 234/477 (49%), Gaps = 31/477 (6%) Query: 26 EVDKRRTFAIISHPDAGKTTLTEKLLLFGGAIRLAGSVKARKASKHATSDWMEIEKQRGI 85 ++DK R I++H DAGKTT TE++L + G G V + T+DWM EK+RGI Sbjct: 10 DLDKLRNIGIMAHIDAGKTTTTERILYYTGRKHFIGDVD----EGNTTTDWMPQEKERGI 65 Query: 86 SVTSSVMQFDYMGHRVNILDTPGHQDFSEDTYRTLTAADSAVMLIDVAKGVEAQTIKLFQ 145 ++ S+ + G+R+NI+DTPGH DF+ + R L D A+ + D GVE Q+ +++ Sbjct: 66 TIQSAATTCFWKGYRINIIDTPGHVDFTAEVERALRVLDGAIAVFDATAGVEPQSETVWR 125 Query: 146 VCAKRGIPIFTFINKLDREGQSPFELMEELENVLGIRSVPMNWPIGGGRELCGVYDRMKN 205 K +P F+NK+D+ G + +E L L +P+ PIG ++ GV D +K Sbjct: 126 QADKYNVPRIAFMNKMDKVGADFYMAVETLVTKLRANPIPVQMPIGSEKDFQGVIDLIKM 185 Query: 206 QVELFQGDDHSVIKVQKVESYNDPVIREMAGEYLHDQLCQDLELLD-----VAGDAFDYE 260 + + +D SV + + + E L D E+L+ + + Sbjct: 186 KAIYWVSEDGSVYEERDIPEELREEAEMRREEMLEKIAELDEEILEKYLEGEEISEEEIK 245 Query: 261 KVLR-----GELTPVFFGSAINNFGVQTFLDNFLELAPKP------EPRRSNAGDV---E 306 +VLR + PV G+A N G+Q LD ++ P P + R + G+V + Sbjct: 246 RVLRKATIENKAVPVLCGAAKANKGIQPLLDAVIDYLPSPLDLPPVKGWRVSDGEVVYRK 305 Query: 307 P-TNEKFTGYVFKIQANMNPAHRDRIAFLRIVSGKFERGMSVKHVRAGKDIKLSQPQQFL 365 P NE FT VFK+Q + + ++ + R+ SG+ E+G V + G+ ++S+ Sbjct: 306 PDENEPFTALVFKVQVD---PYIGKLVYFRVYSGRLEKGSYVYNSTKGQRERISRIVFMH 362 Query: 366 AQDRDIVEEAFPGDIIGLFDPGIFRIGDTLSQAGDIEFDELPTFSPEIFSKVSIKNALKS 425 A R+ V+ PGDI + + GDTL + E F PE ++++ K+ Sbjct: 363 ADKREEVDYVRPGDIAAGVGLKVSQTGDTLCDEKEPIILEKIDF-PEPVISLAVEPVTKA 421 Query: 426 --KQFQKGIDQLTEEG-MIQVFRTVNFDDILLGVVGQLQFEVFEYRMKGEYGVDVQL 479 ++ K + L+EE +QV + ++ +G+L E+ R+K E+GV+V++ Sbjct: 422 DEEKLVKALLALSEEDPTLQVRVDKETGETIISGMGELHLEIVVDRLKREFGVNVRV 478 >YLR069c [J] COG0480 Translation elongation factors (GTPases) Length = 761 Score = 184 bits (466), Expect = 4e-46, Method: Compositional matrix adjust. Identities = 138/483 (28%), Positives = 240/483 (49%), Gaps = 45/483 (9%) Query: 27 VDKRRTFAIISHPDAGKTTLTEKLLLFGGAIRLAGSVKARKASKHATSDWMEIEKQRGIS 86 +K R I +H D+GKTT TE++L + I+ V+ R + A D M++E+++GI+ Sbjct: 67 CNKLRNIGISAHIDSGKTTFTERVLYYTKRIKAIHEVRGRD-NVGAKMDSMDLEREKGIT 125 Query: 87 VTSSV--MQFDYMG--HRVNILDTPGHQDFSEDTYRTLTAADSAVMLIDVAKGVEAQTIK 142 + S+ +D G + N++DTPGH DF+ + R L D AV+++ GV++QT+ Sbjct: 126 IQSAATYCSWDKEGKNYHFNLIDTPGHIDFTIEVERALRVLDGAVLVVCAVSGVQSQTVT 185 Query: 143 LFQVCAKRGIPIFTFINKLDREGQSPFELMEELENVLGIRSVPMNWPIGGGRELCGVYDR 202 + + + +P TFINK+DR G PF +E+L + L I + + PIG L GV D Sbjct: 186 VDRQMRRYNVPRVTFINKMDRMGSDPFRAIEQLNSKLKIPAAAVQIPIGSESSLSGVVD- 244 Query: 203 MKNQVELF-QGDDHSVIKVQKVESYNDPVI---REMAGEYLHDQLCQDLELL-------- 250 + N+V ++ +GD+ +I+ V P++ R++ E L D + E+ Sbjct: 245 LINRVAIYNKGDNGEIIEKGPVPENLKPLMEEKRQLLIETLADVDDEMAEMFLEEKEPTT 304 Query: 251 DVAGDAFDYEKVLRGELTPVFFGSAINNFGVQTFLDNFLELAPKPEPRRSNAGDVEPTNE 310 DA + R TPV GSA+ N G+Q LD ++ P P + A DV Sbjct: 305 QQIKDAIRRSTIARS-FTPVLMGSALANTGIQPVLDAIVDYLPNPSEVLNTALDVSNNEA 363 Query: 311 K----------FTGYVFKIQANMNPAHRDRIAFLRIVSGKFERGMSVKHVRAGKDIKLSQ 360 K F G FK++ ++ ++R+ G+ +G + +V+ GK +K+++ Sbjct: 364 KVNLVPAVQQPFVGLAFKLEE----GKYGQLTYVRVYQGRLRKGNYITNVKTGKKVKVAR 419 Query: 361 PQQFLAQDRDIVEEAFPGDIIGLFDPGI-FRIGDTLSQAGDIEFDELPTFSPEIFSKVSI 419 + + + + V+E G+I F GI GDT + G +++ + P+ +SI Sbjct: 420 LVRMHSSEMEDVDEVGSGEICATF--GIDCASGDTFTD-GSVQYSMSSMYVPDAVVSLSI 476 Query: 420 K-NALKSKQFQKGIDQLTEEGMIQVFRTVNFD----DILLGVVGQLQFEVFEYRMKGEYG 474 N+ + F K +++ +E FR V FD + ++ +G+L E++ RM+ EY Sbjct: 477 TPNSKDASNFSKALNRFQKED--PTFR-VKFDPESKETIISGMGELHLEIYVERMRREYN 533 Query: 475 VDV 477 VD Sbjct: 534 VDC 536 >BB0540 [J] COG0480 Translation elongation factors (GTPases) Length = 693 Score = 175 bits (444), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 135/477 (28%), Positives = 230/477 (48%), Gaps = 36/477 (7%) Query: 28 DKRRTFAIISHPDAGKTTLTEKLLLFGGAIRLAGSVKARKASKHATSDWMEIEKQRGISV 87 +K R I +H D+GKTTLTE++L + I VK + AT D ME+E++RGI++ Sbjct: 4 NKLRNIGISAHIDSGKTTLTERILFYCNKIHAIHEVKGKDGVG-ATMDSMELERERGITI 62 Query: 88 TSSVMQFDYMGHRVNILDTPGHQDFSEDTYRTLTAADSAVMLIDVAKGVEAQTIKLFQVC 147 S+ ++ +NI+DTPGH DF+ + R+L D A++++D GV++Q+I + + Sbjct: 63 ASAATHVEWKDFPINIIDTPGHVDFTIEVERSLRVLDGAILVLDSVAGVQSQSITVDRQL 122 Query: 148 AKRGIPIFTFINKLDREGQSPFELMEELENVLGIRSVPMNWPIGGGRELCGVYDRMKNQV 207 + +P F+NK D+ G +P+ + ++L + L + SV M PIG + GV D + + Sbjct: 123 KRYSVPRLAFVNKCDKTGANPYNVKDQLRSKLDLNSVLMQIPIGLEDKHIGVIDLVLMKA 182 Query: 208 ELFQGDDHSVIKVQKVES---YNDPVIREMAGEYLHDQLCQDLELLDVAGDAFDYEKV-- 262 F+G D + I +++ S RE+ + L D +L L + G E + Sbjct: 183 YYFEGKDGTEIIEKEIPSDLLEEAKSKREIMLDTLAD-FNDELMELHMEGKEVPTEIIYN 241 Query: 263 ------LRGELTPVFFGSAINNFGVQTFLDNFLELAPKPEPRRSNAGDVEPTNEK----- 311 L +L PVF GSA N GVQ LD P P ++ A D+ NEK Sbjct: 242 ATRTGTLALKLCPVFMGSAYKNKGVQLLLDAVTRFLPSPHDIKNTALDLN-NNEKEIDLK 300 Query: 312 ------FTGYVFKIQANMNPAHRDRIAFLRIVSGKFERGMSVKHVRAGKDIKLSQPQQFL 365 FK++ ++ ++RI G ++G + + R K K+ + + Sbjct: 301 IDNELPTVALAFKLE----DGQYGQLTYVRIYQGILKKGQELINSRTSKKFKVGRLIRMH 356 Query: 366 AQDRDIVEEAFPGDIIGLFDPGI-FRIGDTLSQAGDIEFDELPTFSPEIFSKVSIKNALK 424 A + + +E GDI+ LF GI GDT I + F P+ +S+K K Sbjct: 357 ANNTEDIEFGGSGDIVALF--GIECASGDTFCDP-SINYSMTSMFIPDPVISLSVKPKDK 413 Query: 425 --SKQFQKGIDQLT-EEGMIQVFRTVNFDDILLGVVGQLQFEVFEYRMKGEYGVDVQ 478 + K + + T E+ + + + ++ ++ +G+L EV+ RMK E+ +V+ Sbjct: 414 KSADNMAKALGRFTKEDPTFKTYVDIESNETIIQGMGELHLEVYIERMKREFKAEVE 470 >FN1556 [J] COG0480 Translation elongation factors (GTPases) Length = 693 Score = 175 bits (443), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 143/480 (29%), Positives = 222/480 (46%), Gaps = 42/480 (8%) Query: 27 VDKRRTFAIISHPDAGKTTLTEKLLLFGGAIRLAGSVKARKASKHATSDWMEIEKQRGIS 86 +D R I++H DAGKTT TE++L + G R V AT DWME E++RGI+ Sbjct: 7 LDMTRNVGIMAHIDAGKTTTTERILFYTGVERKLKEVH----EGQATMDWMEQEQERGIT 62 Query: 87 VTSSVMQFDYMGHRVNILDTPGHQDFSEDTYRTLTAADSAVMLIDVAKGVEAQTIKLFQV 146 +TS+ + GHR+NI+DTPGH DF+ + R+L D AV + GV+ Q+ +++ Sbjct: 63 ITSAATTCFWKGHRINIIDTPGHVDFTVEVERSLRVLDGAVAVFSAVDGVQPQSETVWRQ 122 Query: 147 CAKRGIPIFTFINKLDREGQSPFELMEELENVLGIRSVPMNWPIGGGRELCGVYD--RMK 204 K +P F NK+DR G + + +++ LG VP+ PIG + GV D MK Sbjct: 123 ADKYKVPRLAFFNKMDRIGANFDMCVSDIKEKLGSNPVPIQIPIGAEDQFEGVVDLIEMK 182 Query: 205 NQVELFQGDDHSVIKVQKVESYNDPVIREMAGEYLHDQLCQDLELLD--VAGDAFDYEKV 262 V D V+ + + E L + D L++ G+ E++ Sbjct: 183 EIVWPVDSDQGQHFDVKDIRAELKEKAEETRQYMLESIVETDDALMEKFFGGEEITKEEI 242 Query: 263 LRG--------ELTPVFFGSAINNFGVQTFLDNFLELAPKP------EPRRSNAGDVEPT 308 ++G + PV G+A N G+Q LD + P P E R DV Sbjct: 243 IKGLRKATIDNTIVPVVCGTAFKNKGIQALLDAIVNYMPAPTDVAMVEGRDPKNPDVLID 302 Query: 309 NE-----KFTGYVFKIQANMNPAHRDRIAFLRIVSGKFERGMSVKHVRAGKDIKLSQPQQ 363 E F FK+ M R+ F R+ SG E+G +V + GK ++ + Q Sbjct: 303 REMSDDAPFAALAFKV---MTDPFVGRLTFFRVYSGFVEKGATVLNSTKGKKERMGRILQ 359 Query: 364 FLAQDRDIVEEAFPGDI---IGLFDPGIFRIGDTL-SQAGDIEFDELPTFSPEIFSKVSI 419 A +R+ +E + GDI +GL D GDTL ++ I +++ PE V++ Sbjct: 360 MHANNREEIEHVYCGDIAAAVGLKDTA---TGDTLCAENAPIVLEQMEF--PEPVISVAV 414 Query: 420 KNALKSKQFQKGI--DQLTEEGMIQVFRT-VNFDDILLGVVGQLQFEVFEYRMKGEYGVD 476 + K+ Q + GI +L EE RT ++ +G+L E+ RMK E+ V+ Sbjct: 415 EPKTKNDQEKMGIALSKLAEEDPTFKVRTDEETGQTIISGMGELHLEIIVDRMKREFKVE 474 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.137 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 39,699,064 Number of extensions: 1733785 Number of successful extensions: 14944 Number of sequences better than 1.0e-05: 110 Number of HSP's gapped: 14753 Number of HSP's successfully gapped: 110 Length of query: 542 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 431 Effective length of database: 25,576,357 Effective search space: 11023409867 Effective search space used: 11023409867 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 118 (50.1 bits)