BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0391.0032.p (300 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM1028 [R] COG3772 Phage-related lysozyme (muraminidase) 142 9e-34 TM1028 [V] COG1131 ABC-type multidrug transport system, ATPase c... 142 9e-34 PAB1845 [V] COG1131 ABC-type multidrug transport system, ATPase ... 136 4e-32 BH0818 [V] COG1131 ABC-type multidrug transport system, ATPase c... 135 7e-32 AF1136 [V] COG1131 ABC-type multidrug transport system, ATPase c... 135 1e-31 >STM1028 [R] COG3772 Phage-related lysozyme (muraminidase) Length = 150 Score = 142 bits (357), Expect = 9e-34, Method: Compositional matrix adjust. Identities = 0/286 (0%), Positives = 0/286 (0%), Gaps = 4/286 (1%) Query: 1 MIEIRGVSKSFQGEKAVDSLSLTVHKGAIYGLLGSNGAGKTTLLKTLAGIYRSEEGTVKI 60 I +G++ + E + G +G G+ E ++ Sbjct: 3 RISEKGITLIKEFEGCSLTAYPDPGTGGDPWTIGYGWTHSVDGKPVKPGMMIDEATAERL 62 Query: 61 GGQPVF-ENPQVKQSLIFMPDSPYFFPQASLKTMAAFYRSVYPGFSDKRFEELGTVFRLD 119 + V + + F S K + Sbjct: 63 LNTGLVGYENDVSRLVKVKLTQGQFDALVSFAYNLGARTLSSSTLLRKLNAGDYAGAADE 122 Query: 120 MGRKLSRFSKGMQRQAAFWLALSCRPDVLIMDEPIDGLDPVMRRQIKNLLFQEVAERELT 179 Sbjct: 123 - 181 Query: 180 VLISSHNLREIEDLCDHVGIMHHGRMLVEKDLDDLKADTHKVQVAFRDERHADALAAKLQ 239 Sbjct: 182 -- 239 Query: 240 ILHQERRGSVDLYIVKGDRERISKVIHVYEPYVFDLLPLTLEEIFI 285 Sbjct: 240 285 >TM1028 [V] COG1131 ABC-type multidrug transport system, ATPase component Length = 293 Score = 142 bits (357), Expect = 9e-34, Method: Compositional matrix adjust. Identities = 83/286 (29%), Positives = 160/286 (55%), Gaps = 4/286 (1%) Query: 1 MIEIRGVSKSFQGEKAVDSLSLTVHKGAIYGLLGSNGAGKTTLLKTLAGIYRSEEGTVKI 60 ++E+R ++K + + V+ ++ +V +G I+G +G NGAGKTT ++ L G+ + G +I Sbjct: 3 VVEVRDLTKYYGKSRGVEGVTFSVEEGEIFGFIGPNGAGKTTTIRLLLGLIFPDSGLAEI 62 Query: 61 GGQPVF-ENPQVKQSLIFMPDSPYFFPQASLKTMAAFYRSVYPGFSDKRFEELGTVFRLD 119 G+ V E ++++++ ++P F+P+ +++ + S Y +EL VF LD Sbjct: 63 FGKDVLKEGKEIRKNVGYIPGEVSFYPEVTVEEFLRYSASFYEKVDWDYVKELCGVFSLD 122 Query: 120 MGRKLSRFSKGMQRQAAFWLALSCRPDVLIMDEPIDGLDPVMRRQIKNLLFQEVAERELT 179 +++ S G +++ A AL RP +LI+DEP +GLDP+++ +L E E+ T Sbjct: 123 TKKRIKELSMGNKKKVAIVQALMHRPKLLILDEPTNGLDPIVQNTFFEILKNE-KEKGTT 181 Query: 180 VLISSHNLREIEDLCDHVGIMHHGRMLVEKDLDDLKADTHKVQVAFRDERHADALAAKLQ 239 V SSH L E+E LCD V ++ GR++ + +++LK + +KV V + E + + L + Sbjct: 182 VFFSSHILSEVERLCDRVAMIKDGRIIRVEKVENLKGERYKV-VRVKGE-NLEKLKDLAE 239 Query: 240 ILHQERRGSVDLYIVKGDRERISKVIHVYEPYVFDLLPLTLEEIFI 285 + + ++ G+ ER+ +++ + F + +LEEIF+ Sbjct: 240 VNRLKFENGTAEFLFSGNVERLLEILRSLKLSDFWVEEPSLEEIFM 285 >PAB1845 [V] COG1131 ABC-type multidrug transport system, ATPase component Length = 305 Score = 136 bits (343), Expect = 4e-32, Method: Compositional matrix adjust. Identities = 85/222 (38%), Positives = 137/222 (61%), Gaps = 9/222 (4%) Query: 1 MIEIRGVSKSFQGEKAVDSLSLTVHKGAIYGLLGSNGAGKTTLLKTLAGIYRSEEGTVKI 60 +IEI ++KS+ KAVD+LSL V KG I+G LG NGAGKTT + ++ GI + G+V++ Sbjct: 3 VIEIENLTKSYGKFKAVDNLSLEVEKGIIFGFLGPNGAGKTTTILSMLGIIIPDSGSVRL 62 Query: 61 GGQPVFENP-QVKQSLIFMPDSPYFFPQAS----LKTMAAFYRSVYPGFSDKRFEELGTV 115 G +F+ P + K+ L F+P++ + + + L A+FY ++ +KR EEL + Sbjct: 63 LGYDIFKEPIKAKERLGFLPENATIYEELTAWRNLDFFASFY-NIPKQEKEKRIEELLKL 121 Query: 116 FRL-DMG-RKLSRFSKGMQRQAAFWLALSCRPDVLIMDEPIDGLDPVMRRQIKNLLFQEV 173 L D+ RK+ FSKGM+++ AL P++LI+DEP GLDP R +K+++ ++ Sbjct: 122 VGLWDVRYRKVKSFSKGMKQRLLLAQALINDPELLILDEPTSGLDPQGARLVKDIILEQK 181 Query: 174 AERELTVLISSHNLREIEDLCDHVGIMHHGRMLVEKDLDDLK 215 + + TV SSH L E+E+L D VGI+ G++ L+++K Sbjct: 182 KQGK-TVFFSSHILSEVEELADKVGIIVKGKLRTVGTLEEIK 222 >BH0818 [V] COG1131 ABC-type multidrug transport system, ATPase component Length = 314 Score = 135 bits (341), Expect = 7e-32, Method: Compositional matrix adjust. Identities = 77/235 (32%), Positives = 137/235 (58%), Gaps = 4/235 (1%) Query: 1 MIEIRGVSKSFQGEKAVDSLSLTVHKGAIYGLLGSNGAGKTTLLKTLAGIYRSEEGTVKI 60 +I R ++K+FQG++ + +L+L V+KG IYG LG NGAGKTT++K L + G +++ Sbjct: 4 VITTRNLTKTFQGKEIISNLNLHVNKGEIYGFLGPNGAGKTTVMKMLMNFVKPSAGEIEL 63 Query: 61 GGQPVF-ENPQVKQSLIFMPDSPYFFPQASLKTMAAFYRSVYPGFSDKRFEELGTVFRLD 119 GQ + ++ ++ + + M + P F+ + S + + + EE + +L Sbjct: 64 FGQRLTSQSYEMLKRIGSMIEFPIFYEKLSARENLELHCEYMGYYKPSAIEEALEMVKLQ 123 Query: 120 M--GRKLSRFSKGMQRQAAFWLALSCRPDVLIMDEPIDGLDPVMRRQIKNLLFQEVAERE 177 + + + FS GM+++ A+ +P++LI+DEPI+GLDPV ++I+ L E Sbjct: 124 LTGSKAVKDFSLGMKQRLGIARAICTKPELLILDEPINGLDPVGIKEIRQLFQTLCNEYG 183 Query: 178 LTVLISSHNLREIEDLCDHVGIMHHGRMLVEKDLDDLKA-DTHKVQVAFRDERHA 231 +T+LISSH L EIE + D VG++ +G ++ E LDD+++ T +++ D R A Sbjct: 184 VTILISSHILSEIEQIADTVGVIRNGMLVAEVALDDIRSQQTEYIELLTYDARKA 238 >AF1136 [V] COG1131 ABC-type multidrug transport system, ATPase component Length = 294 Score = 135 bits (339), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 91/298 (30%), Positives = 161/298 (54%), Gaps = 16/298 (5%) Query: 2 IEIRGVSKSFQGEKAVDSLSLTVHKGAIYGLLGSNGAGKTTLLKTLAGIYRSEEGTVKIG 61 IE+ ++K F G +A+D +SL V +G I+G LG NGAGKTT + G+ + G+V+I Sbjct: 4 IEVENLTKVFNGFRALDGISLVVERGEIFGFLGPNGAGKTTTINCCLGLIKPTSGSVRIA 63 Query: 62 GQPVFENP-QVKQSLIFMPDSPYFFPQASLKTMAAFYRSVYPGFSDKRFEELGTVFRLDM 120 G V E P +VK+ ++P++ F+P + + ++ Y D E L V D Sbjct: 64 GMDVEEKPVEVKEICGYLPENYGFYPNLTARQNLLYFSEFYANPKDNVDELLALVGLQDS 123 Query: 121 G-RKLSRFSKGMQRQAAFWLALSCRPDVLIMDEPIDGLDPVMRRQIKNLLFQEVAERELT 179 +K+ FS+GM+++ A AL P+V+ +DEP +GLDP + ++ +E+ ++ T Sbjct: 124 ADKKVGEFSRGMKQRLALAQALINDPEVVFLDEPTNGLDPQGIADFRKII-RELNQKGKT 182 Query: 180 VLISSHNLREIEDLCDHVGIMHHGRMLVEKDLDDLKADTHKVQVAFRDERHADA--LAAK 237 SSH L E+ ++C VGI++ GR++ + +L + ++V + E AD L Sbjct: 183 FFFSSHILSEVREVCSVVGIINRGRIVKVGKISEL---SGSMEVVVQTEPRADESLLREF 239 Query: 238 LQILHQERRGSVDLYIVKGD---RERISKVI--HVYEPYVFDLLPLTLEEIFIYEMGD 290 ++ + E R D+Y++K + R IS+ + H + L +LE+I++ +G+ Sbjct: 240 GEVEYDESR---DVYVIKVESDCRVEISRTLFEHGFVVKELSLREPSLEKIYLSMVGE 294 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.140 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,670,008 Number of extensions: 880985 Number of successful extensions: 8610 Number of sequences better than 1.0e-05: 1775 Number of HSP's gapped: 7088 Number of HSP's successfully gapped: 1903 Length of query: 300 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 194 Effective length of database: 26,153,027 Effective search space: 5073687238 Effective search space used: 5073687238 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 115 (48.9 bits)