BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0391.0022.p (227 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value VCA0608 [R] COG1011 Predicted hydrolase (HAD superfamily) 166 3e-41 STM4559 [R] COG1011 Predicted hydrolase (HAD superfamily) 157 1e-38 BS_yfnB [R] COG1011 Predicted hydrolase (HAD superfamily) 157 1e-38 ZyjjG [R] COG1011 Predicted hydrolase (HAD superfamily) 154 1e-37 yjjG [R] COG1011 Predicted hydrolase (HAD superfamily) 154 1e-37 >VCA0608 [R] COG1011 Predicted hydrolase (HAD superfamily) Length = 224 Score = 166 bits (420), Expect = 3e-41, Method: Compositional matrix adjust. Identities = 93/226 (41%), Positives = 136/226 (60%), Gaps = 6/226 (2%) Query: 1 MKYEVILFDADDTLFDYRMAESRALYNAFAHFGLPTGAEDYAASYKEINAALWKDLEQGR 60 MKY+ ILFDAD+TLF + + L FA G+ +D+ Y+E+N LW D + G Sbjct: 1 MKYDWILFDADETLFHFDAFKGMQLM--FARKGVEFTEQDFH-QYQEVNKPLWVDYQNGD 57 Query: 61 TTSAVLRVERFNRLFAAHKLDLSPEAFSEAYLKFLGEGTFLIQGAVELCRELAG-CRLAI 119 T+A L+ RF A KL+ + + + A+L+ + + L+ GA+EL + L G RL I Sbjct: 58 ITAAQLKHTRFAGW--AEKLNTTTDELNSAFLQAMADICTLLPGAMELMQALQGKARLGI 115 Query: 120 ITNGIKDVQLSRIQGSPLSSTFEQIIISEEAGCQKPERGIFDYAFAKLGISDRSKVLIVG 179 ITNG ++Q R+ + ++ F+QI+ISEE G KP+ GIF +A ++G +S+VL+VG Sbjct: 116 ITNGFTELQDVRLAKTGMTDFFDQIVISEEVGIAKPDAGIFAHALERMGNPTKSRVLMVG 175 Query: 180 DSLTSDIQGGINYGIDTCWFNPLGKEGAPGIHPTYEIRELSELLEI 225 D+ SDI GG+N+GI+TCW N GI P YE+ L EL EI Sbjct: 176 DNPHSDILGGLNFGIETCWLNVHQHPKPDGITPHYEVTSLHELREI 221 >STM4559 [R] COG1011 Predicted hydrolase (HAD superfamily) Length = 226 Score = 157 bits (397), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 86/226 (38%), Positives = 131/226 (57%), Gaps = 6/226 (2%) Query: 1 MKYEVILFDADDTLFDYRMAESRALYNAFAHFGLPTGAEDYAASYKEINAALWKDLEQGR 60 MK++ I FDAD+TLF + L F + + AED+ Y+ +N LW D + G Sbjct: 2 MKWDWIFFDADETLFTFD--SFTGLQRMFLDYSVTFTAEDFQ-DYQAVNKPLWVDYQNGA 58 Query: 61 TTSAVLRVERFNRLFAAHKLDLSPEAFSEAYLKFLGEGTFLIQGAVELCRELAG-CRLAI 119 TS L+ RF A +L+++P ++A++ + E + GAV L + G ++ I Sbjct: 59 ITSLQLQHARFQSW--AERLNVAPGLLNDAFISAMAEICSPLPGAVSLLNAIRGQAKIGI 116 Query: 120 ITNGIKDVQLSRIQGSPLSSTFEQIIISEEAGCQKPERGIFDYAFAKLGISDRSKVLIVG 179 ITNG +Q +R++ + L F+ ++ISE+ G KP+ IF+YA + G DRS+VL+VG Sbjct: 117 ITNGFTALQQTRLERTGLREYFDLLVISEQVGVAKPDPKIFNYALEQAGNPDRSRVLMVG 176 Query: 180 DSLTSDIQGGINYGIDTCWFNPLGKEGAPGIHPTYEIRELSELLEI 225 D+ SDI GGIN G+ TCW N +E GIHPT+ + LSEL ++ Sbjct: 177 DTAESDILGGINAGLSTCWLNAHHREQPAGIHPTWTVASLSELEQL 222 >BS_yfnB [R] COG1011 Predicted hydrolase (HAD superfamily) Length = 235 Score = 157 bits (397), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 90/225 (40%), Positives = 123/225 (54%), Gaps = 2/225 (0%) Query: 2 KYEVILFDADDTLFDYRMAESRALYNAFAHFGLPTGAEDYAASYKEINAALWKDLEQGRT 61 +Y +LFD DDT+ D++ AE+ AL F +P D A YK IN LW+ E+G+ Sbjct: 3 RYRTLLFDVDDTILDFQAAEALALRLLFEDQNIPL-TNDMKAQYKTINQGLWRAFEEGKM 61 Query: 62 TSAVLRVERFNRLFAAHKLDLSPEAFSEAYLKFLGEGTFLIQGAVELCRELAG-CRLAII 120 T + RF+ L + + + Y +FL EG LI GA +L L L I+ Sbjct: 62 TRDEVVNTRFSALLKEYGYEADGALLEQKYRRFLEEGHQLIDGAFDLISNLQQQFDLYIV 121 Query: 121 TNGIKDVQLSRIQGSPLSSTFEQIIISEEAGCQKPERGIFDYAFAKLGISDRSKVLIVGD 180 TNG+ Q R++ S L F+ I +SE+ G QKP + F+Y F ++ LI+GD Sbjct: 122 TNGVSHTQYKRLRDSGLFPFFKDIFVSEDTGFQKPMKEYFNYVFERIPQFSAEHTLIIGD 181 Query: 181 SLTSDIQGGINYGIDTCWFNPLGKEGAPGIHPTYEIRELSELLEI 225 SLT+DI+GG G+DTCW NP K P I PTYEIR+L EL I Sbjct: 182 SLTADIKGGQLAGLDTCWMNPDMKPNVPEIIPTYEIRKLEELYHI 226 >ZyjjG [R] COG1011 Predicted hydrolase (HAD superfamily) Length = 225 Score = 154 bits (389), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 87/226 (38%), Positives = 127/226 (56%), Gaps = 6/226 (2%) Query: 1 MKYEVILFDADDTLFDYRMAESRALYNAFAHFGLPTGAEDYAASYKEINAALWKDLEQGR 60 MK++ I FDAD+TLF + L F + + AED+ Y+ +N LW D + G Sbjct: 1 MKWDWIFFDADETLFTFD--SFTGLQRMFLDYSVTFTAEDFQ-DYQAVNKPLWVDYQNGA 57 Query: 61 TTSAVLRVERFNRLFAAHKLDLSPEAFSEAYLKFLGEGTFLIQGAVELCRELAG-CRLAI 119 TS L+ RF A +L++ P +EA++ + E + GAV L + G ++ I Sbjct: 58 ITSLQLQHGRFESW--AERLNVEPGKLNEAFINAMAEICTPLPGAVSLLNAIRGNAKIGI 115 Query: 120 ITNGIKDVQLSRIQGSPLSSTFEQIIISEEAGCQKPERGIFDYAFAKLGISDRSKVLIVG 179 ITNG +Q R++ + L F+ ++ISEE G KP + IFDYA + G DRS+VL+VG Sbjct: 116 ITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYALEQAGNPDRSRVLMVG 175 Query: 180 DSLTSDIQGGINYGIDTCWFNPLGKEGAPGIHPTYEIRELSELLEI 225 D+ SDI GGIN G+ TCW N +E GI PT+ + L EL ++ Sbjct: 176 DTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHELEQL 221 >yjjG [R] COG1011 Predicted hydrolase (HAD superfamily) Length = 225 Score = 154 bits (389), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 87/226 (38%), Positives = 127/226 (56%), Gaps = 6/226 (2%) Query: 1 MKYEVILFDADDTLFDYRMAESRALYNAFAHFGLPTGAEDYAASYKEINAALWKDLEQGR 60 MK++ I FDAD+TLF + L F + + AED+ Y+ +N LW D + G Sbjct: 1 MKWDWIFFDADETLFTFD--SFTGLQRMFLDYSVTFTAEDFQ-DYQAVNKPLWVDYQNGA 57 Query: 61 TTSAVLRVERFNRLFAAHKLDLSPEAFSEAYLKFLGEGTFLIQGAVELCRELAG-CRLAI 119 TS L+ RF A +L++ P +EA++ + E + GAV L + G ++ I Sbjct: 58 ITSLQLQHGRFESW--AERLNVEPGKLNEAFINAMAEICTPLPGAVSLLNAIRGNAKIGI 115 Query: 120 ITNGIKDVQLSRIQGSPLSSTFEQIIISEEAGCQKPERGIFDYAFAKLGISDRSKVLIVG 179 ITNG +Q R++ + L F+ ++ISEE G KP + IFDYA + G DRS+VL+VG Sbjct: 116 ITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYALEQAGNPDRSRVLMVG 175 Query: 180 DSLTSDIQGGINYGIDTCWFNPLGKEGAPGIHPTYEIRELSELLEI 225 D+ SDI GGIN G+ TCW N +E GI PT+ + L EL ++ Sbjct: 176 DTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHELEQL 221 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.139 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 16,591,094 Number of extensions: 684642 Number of successful extensions: 1329 Number of sequences better than 1.0e-05: 30 Number of HSP's gapped: 1294 Number of HSP's successfully gapped: 30 Length of query: 227 Length of database: 38,378,431 Length adjustment: 103 Effective length of query: 124 Effective length of database: 26,499,029 Effective search space: 3285879596 Effective search space used: 3285879596 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 114 (48.5 bits)