BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0391.0020.p (337 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2175 [E] COG0346 Lactoylglutathione lyase and related lyases 449 e-126 BS_ykcA [E] COG0346 Lactoylglutathione lyase and related lyases 362 e-100 lin0753 [E] COG0346 Lactoylglutathione lyase and related lyases 356 2e-98 lin0292 [E] COG0346 Lactoylglutathione lyase and related lyases 324 1e-88 SA2307 [E] COG0346 Lactoylglutathione lyase and related lyases 292 7e-79 >BH2175 [E] COG0346 Lactoylglutathione lyase and related lyases Length = 327 Score = 449 bits (1155), Expect = e-126, Method: Compositional matrix adjust. Identities = 214/321 (66%), Positives = 260/321 (80%) Query: 15 LKGLHHVSALTARAPENFKFYTEVLGLRLIKKTVNQDDVSVYHLFYGDEAGNPGTEVTFF 74 LKG HHVSA+TA+A EN+ FYT+ LG+RL+KKTVNQDD S+YHLFY DE GNPGT++TFF Sbjct: 6 LKGQHHVSAITAKAKENYTFYTKTLGMRLVKKTVNQDDTSMYHLFYADERGNPGTDLTFF 65 Query: 75 EIPNAGRNRDGNNSISALSLRVPDDKALEYWTQRLAEFQVEHDEIQDRGGRKTLAFRDHE 134 EIP+AG+ G NSI+ SLRVP D+AL+YW +R EF +EHD + GR TLAFRD E Sbjct: 66 EIPHAGQTYQGTNSITKTSLRVPSDEALQYWLKRFDEFGIEHDGVSHHTGRATLAFRDFE 125 Query: 135 GQRLILVSDEHNQGVAGGKPWDKSPVPAAYGILGLGPVHLTVPAAESSAKVLVELLGFRR 194 GQRL LVSDE+N+GVA G PW +SPVP AY I GLGPV LTV E + K+L++++G+R Sbjct: 126 GQRLELVSDENNEGVASGIPWKESPVPEAYAIRGLGPVTLTVSRPELTEKILIDVMGYRE 185 Query: 195 KSTYPSLVAGQPDVIVFETGEGGSGAELHLEERTDLPQERLGRGGVHHVAFRVDNEEELK 254 K +YPSLV GQPDV VFETGEGG+GAE+HLE R D+ +ER GRG VHHVAFRV+++EEL+ Sbjct: 186 KGSYPSLVEGQPDVKVFETGEGGTGAEVHLETRKDIGRERPGRGSVHHVAFRVESKEELE 245 Query: 255 QWIGRISSYSLPNSGFVDRFYFRSLYFREPSGILFELATDGPGFATDEDIEHLGESLALP 314 +W+ I++ LPNSGFV+R+YFRSLYFREP+GILFELATDGPGF DE E LGESLALP Sbjct: 246 KWVDYITNIGLPNSGFVERYYFRSLYFREPNGILFELATDGPGFEGDEPFETLGESLALP 305 Query: 315 PFLEDKRPQIEAHLKPLDTRQ 335 P+LE +R IE +LKPLDT Q Sbjct: 306 PYLEPQRETIEKNLKPLDTAQ 326 >BS_ykcA [E] COG0346 Lactoylglutathione lyase and related lyases Length = 316 Score = 362 bits (929), Expect = e-100, Method: Compositional matrix adjust. Identities = 178/319 (55%), Positives = 227/319 (71%), Gaps = 4/319 (1%) Query: 13 LTLKGLHHVSALTARAPENFKFYTEVLGLRLIKKTVNQDDVSVYHLFYGDEAGNPGTEVT 72 + + G+HHVSALTA A +N FY +VLGL+L+KK+VNQD+ ++YHLFYGDE NPGTE+T Sbjct: 1 MKVNGIHHVSALTADAQKNLDFYKKVLGLKLVKKSVNQDEPTMYHLFYGDEVANPGTELT 60 Query: 73 FFEIPNAGRNRDGNNSISALSLRVPDDKALEYWTQRLAEFQVEHDEIQDRGGRKTLAFRD 132 FFEIP G NSIS++ LRVP +AL YW +R E QV H I R GR LAF+D Sbjct: 61 FFEIPRIAPFHAGTNSISSIGLRVPGTEALHYWKERFEEQQVTHSGISKRAGRDILAFQD 120 Query: 133 HEGQRLILVSDEHNQGVAGGKPWDKSPVPAAYGILGLGPVHLTVPAAESSAKVLVELLGF 192 HEGQRL+L +DE +G G P +S +P + GLGPV LTVP AE + VL +LGF Sbjct: 121 HEGQRLVLTADEEGKGY--GLPVKQSGIPEEFSFRGLGPVELTVPYAEPTLHVLTNILGF 178 Query: 193 RRKSTYPSLVAGQPDVIVFETGEGGSGAELHLEERTDLPQERLGRGGVHHVAFRVDNEEE 252 S P V GQ ++ E+GEGG+ E+HL ER DLP+ER G+G VHHVAFRV +EEE Sbjct: 179 TEISREP--VEGQGTAVILESGEGGAATEIHLIERNDLPRERQGKGSVHHVAFRVRDEEE 236 Query: 253 LKQWIGRISSYSLPNSGFVDRFYFRSLYFREPSGILFELATDGPGFATDEDIEHLGESLA 312 L W IS NSG V+R+YF++LYFREP+GILFEL+TDGPGF DE+++ LG+++A Sbjct: 237 LAGWHRIISREGFSNSGIVERYYFKALYFREPNGILFELSTDGPGFMVDENLDELGQTIA 296 Query: 313 LPPFLEDKRPQIEAHLKPL 331 LPP+LE +R +IEA LKP+ Sbjct: 297 LPPYLEHRRAEIEAKLKPI 315 >lin0753 [E] COG0346 Lactoylglutathione lyase and related lyases Length = 319 Score = 356 bits (914), Expect = 2e-98, Method: Compositional matrix adjust. Identities = 170/321 (52%), Positives = 229/321 (71%), Gaps = 7/321 (2%) Query: 15 LKGLHHVSALTARAPENFKFYTEVLGLRLIKKTVNQDDVSVYHLFYGDEAGNPGTEVTFF 74 LKG+HHV+A+T+ A +N+ F+TEVLG+RL+KKTVNQDD+ YHLF+ D+ G+ GT++TFF Sbjct: 5 LKGIHHVTAMTSSAEKNYAFFTEVLGMRLVKKTVNQDDIHTYHLFFADDKGSAGTDMTFF 64 Query: 75 EIPNAGRNRDGNNSISALSLRVPDDKALEYWTQRLAEFQVEHDEIQDRGGRKTLAFRDHE 134 + PN + R G +SIS +S RVP D ALEYW R + +V H +IQ G+K L F+D + Sbjct: 65 DFPNLPKGRHGTDSISRVSFRVPSDAALEYWLDRFDQLEVPHSDIQTLFGKKYLTFQDFD 124 Query: 135 GQRLILVSDEHNQGVAGGKPWDKSPVPAAYGILGLGPVHLTVPAAESSAKVLVELLGFRR 194 Q+L L+SDE+N+GVA G PW K PV Y I GLGPV LTV + ++ +L + GFR+ Sbjct: 125 DQQLQLISDENNEGVAAGTPWKKGPVSEKYAIYGLGPVFLTVVSLKNMEAILQTIFGFRK 184 Query: 195 KSTYPSLVAGQPDVIVFETGEGGSGAELHLEERTDLPQERLGRGGVHHVAFRVDNEEELK 254 V + + ++E GEGG+GA++ +EERTD+P G GGVHHVAFRVD+ EEL+ Sbjct: 185 -------VTEEDGLHLYEVGEGGNGAQVIVEERTDIPAAMQGYGGVHHVAFRVDDHEELQ 237 Query: 255 QWIGRISSYSLPNSGFVDRFYFRSLYFREPSGILFELATDGPGFATDEDIEHLGESLALP 314 +WI R+++ PNSG+V+RFYF SLY ILFE ATDGPGFA DE E LGE LALP Sbjct: 238 KWIDRMNTIEAPNSGYVNRFYFESLYVPVSERILFEFATDGPGFAGDEPYETLGEKLALP 297 Query: 315 PFLEDKRPQIEAHLKPLDTRQ 335 PFLE KR +IE ++P++T++ Sbjct: 298 PFLEPKRAEIEKMVRPINTKR 318 >lin0292 [E] COG0346 Lactoylglutathione lyase and related lyases Length = 319 Score = 324 bits (830), Expect = 1e-88, Method: Compositional matrix adjust. Identities = 166/321 (51%), Positives = 211/321 (65%), Gaps = 4/321 (1%) Query: 13 LTLKGLHHVSALTARAPENFKFYTEVLGLRLIKKTVNQDDVSVYHLFYGDEAGNPGTEVT 72 + L G+HHVS TA A NF FYT+++GLRL+KK+VNQDD YHL+YGDE G+PGT +T Sbjct: 1 MKLAGIHHVSIFTANARANFDFYTKIMGLRLVKKSVNQDDPYTYHLYYGDEIGSPGTALT 60 Query: 73 FFEIPNAGRNRDGNNSISALSLRVPDDKALEYWTQRLAEFQVEHDEIQDRGGRKTLAFRD 132 FFE+PN +N N+IS LSLRVP+D+AL YW +RL E ++ H + D+ GRK + +D Sbjct: 61 FFEVPNMAKNHPSRNAISGLSLRVPNDEALHYWDKRLDEHRIFHSKPIDQFGRKIIRLKD 120 Query: 133 HEGQRLILVSDEHNQGVAGGKPWDKSPVPAAYGILGLGPVHLTVPAAESSAKVLVELLGF 192 +G + L+SDE + W+ SPVPA Y I GLGPV +V E + ++L ++LGF Sbjct: 121 TDGLPVSLISDETSTQFTEVSAWEDSPVPAEYAIRGLGPVRFSVFKKEKTDRLLTKILGF 180 Query: 193 RRKSTYPSLVAGQPDVIVFETGEGGSGAELHLEERTDLPQERLGRGGVHHVAFRVDNEEE 252 R Y V VF+TG+ G G E+H+E R DL Q LG GG+HHVAFRV + + Sbjct: 181 ERIGAYED---DDKLVTVFKTGDVGLGGEVHVESRPDLEQGNLGAGGIHHVAFRVPTDGD 237 Query: 253 LKQWIGRISSYSLPNSGFVDRFYFRSLYFREPSGILFELATDGPGFATDEDIEHLGESLA 312 L W I NSG+VDRFYF SLYFRE +GIL ELATD PGF TD EH G + Sbjct: 238 LIGWTEMIQDLGYKNSGYVDRFYFHSLYFRESNGILIELATDEPGFQTDFTKEH-GTYVD 296 Query: 313 LPPFLEDKRPQIEAHLKPLDT 333 LPP LE++R I AHLKPLDT Sbjct: 297 LPPHLEERREDILAHLKPLDT 317 >SA2307 [E] COG0346 Lactoylglutathione lyase and related lyases Length = 319 Score = 292 bits (747), Expect = 7e-79, Method: Compositional matrix adjust. Identities = 146/321 (45%), Positives = 205/321 (63%), Gaps = 8/321 (2%) Query: 15 LKGLHHVSALTARAPENFKFYTEVLGLRLIKKTVNQDDVSVYHLFYGDEAGNPGTEVTFF 74 L G+HHV+A+T A N+KF+TEVLG+RL+KKTVNQDD+ YH F+ D+ G+ GT++TFF Sbjct: 7 LLGIHHVTAMTDDAERNYKFFTEVLGMRLVKKTVNQDDIYTYHTFFADDVGSAGTDMTFF 66 Query: 75 EIPNAGRNRDGNNSISALSLRVPDDKALEYWTQRLAEFQVEHDEIQDRGGRKTLAFRDHE 134 + PN + + G NSI+ S RVP+D AL Y+ QR EF V+H+ IQ+ G+K L F + + Sbjct: 67 DFPNITKGQAGTNSITRPSFRVPNDDALMYYEQRFDEFGVKHEGIQELFGKKVLPFEEVD 126 Query: 135 GQRLILVSDEHNQGVAGGKPWDKSPVPAAYGILGLGPVHLTVPAAESSAKVLVELLGFRR 194 GQ L+SDE N GVA G PW PVP I GLGP+ + V + +L + G Sbjct: 127 GQVYQLISDELNDGVAPGVPWKNGPVPVDKAIYGLGPIEIKVSYFDDFKNILETVYGM-- 184 Query: 195 KSTYPSLVAGQPDVIVFETGEGGSGAELHLEERTDLPQERLGRGGVHHVAFRVDNEEELK 254 + +A + +V + E GEGG+G ++ L + P R G G VHHV+FRV + + ++ Sbjct: 185 -----TTIAHEDNVALLEVGEGGNGGQVILIKDDKGPAARQGYGEVHHVSFRVKDHDAIE 239 Query: 255 QWIGRISSYSLPNSGFVDRFYFRSLYFREPSGILFELATDGPGFATDEDIEHLGESLALP 314 W + + NSG V+RFYF +LY R IL E++TDGPGF DE E LGE L+LP Sbjct: 240 AWATKYKEVGINNSGIVNRFYFEALYAR-VGHILIEISTDGPGFMEDEPYETLGEGLSLP 298 Query: 315 PFLEDKRPQIEAHLKPLDTRQ 335 PFLE+KR IE+ ++P +T++ Sbjct: 299 PFLENKREYIESEIRPFNTKR 319 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.138 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 27,851,401 Number of extensions: 1299941 Number of successful extensions: 2305 Number of sequences better than 1.0e-05: 13 Number of HSP's gapped: 2268 Number of HSP's successfully gapped: 13 Length of query: 337 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 230 Effective length of database: 26,037,693 Effective search space: 5988669390 Effective search space used: 5988669390 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 116 (49.3 bits)