BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0391.0015.p (215 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ydgI [C] COG0778 Nitroreductase 169 2e-42 lin0151 [C] COG0778 Nitroreductase 169 4e-42 BH2236 [C] COG0778 Nitroreductase 165 6e-41 BH3859 [C] COG0778 Nitroreductase 160 1e-39 BS_yodC [C] COG0778 Nitroreductase 159 4e-39 >BS_ydgI [C] COG0778 Nitroreductase Length = 346 Score = 169 bits (429), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 9/204 (4%), Positives = 30/204 (14%), Gaps = 2/204 (0%) Query: 9 QEFSKVIRERRSVRKYDSSWKISDXXXXXXXXXXXXXPSSSNLQPWRFIVITDQEIKAKL 68 + + + + + + + + + L Sbjct: 5 RIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGL 64 Query: 69 LPIA-HNQAQTTESSAMIAVLGDTEAYSNAEKIYGHASATGLMPEDVAAAMAKRSREGYS 127 N S + + S A R Sbjct: 65 TETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTD 124 Query: 128 SLPKEKLEDIALVDGGLVSMQLMLAAKARGYDTVPMGGYDAAALKELFRIS-DRYIPVML 186 L ++ + A + A + Sbjct: 125 YLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAF 184 Query: 187 IALGKAAVEGRPTNRLPLEAVVHW 210 + + + + + Sbjct: 185 GVMRYLHLADKHDLPRIVTIQNPY 208 >lin0151 [C] COG0778 Nitroreductase Length = 346 Score = 169 bits (427), Expect = 4e-42, Method: Compositional matrix adjust. Identities = 12/206 (5%), Positives = 34/206 (16%), Gaps = 2/206 (0%) Query: 7 VEQEFSKVIRERRSVRKYDSSWKISDXXXXXXXXXXXXXPSSSNLQPWRFIVITDQEIKA 66 S + + + S+ + + V E + Sbjct: 4 HRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQG 63 Query: 67 KLLPIAH-NQAQTTESSAMIAVLGDTEAYSNAEKIYGHASATGLMPEDVAAAMAKRSREG 125 A+ +I + N +K A A + + + Sbjct: 64 LTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQT 123 Query: 126 YSSLPKEKLEDIALVDGGLVSMQLMLAAKARGYDTVPMGGYDAAALKELFRIS-DRYIPV 184 + + + + R Sbjct: 124 DYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETA 183 Query: 185 MLIALGKAAVEGRPTNRLPLEAVVHW 210 + + R+ + Sbjct: 184 FGVMRYLHLADKHDLPRIVTIQNPYS 209 >BH2236 [C] COG0778 Nitroreductase Length = 346 Score = 165 bits (417), Expect = 6e-41, Method: Compositional matrix adjust. Identities = 13/201 (6%), Positives = 36/201 (17%), Gaps = 1/201 (0%) Query: 13 KVIRERRSVRKYDSSWKISDXXXXXXXXXXXXXPSSSNLQPWRFIVITDQEIKAKLLPIA 72 + +S + + + P T + + Sbjct: 14 STLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWL 73 Query: 73 HNQAQTTESSAMIAVLGDTEAYSNAEKIYGHASATGLMPEDVAAAMAKRSREGYSSLPKE 132 + V G + + + + ++ Sbjct: 74 AKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLY-QVH 132 Query: 133 KLEDIALVDGGLVSMQLMLAAKARGYDTVPMGGYDAAALKELFRISDRYIPVMLIALGKA 192 + G L A DT+ A K + ++ Sbjct: 133 WPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHL 192 Query: 193 AVEGRPTNRLPLEAVVHWNGF 213 A + + ++ Sbjct: 193 ADKHDLPRIVTIQNPYSLLNR 213 >BH3859 [C] COG0778 Nitroreductase Length = 346 Score = 160 bits (406), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 15/204 (7%), Positives = 42/204 (20%), Gaps = 2/204 (0%) Query: 9 QEFSKVIRERRSVRKYDSSWKISDXXXXXXXXXXXXXPSSSNLQPWRFIVITDQEIKAKL 68 +V + +D + + + + + Sbjct: 8 HSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTET 67 Query: 69 LPIAH-NQAQTTESSAMIAVLGDTEAYSNAEKIYGHASATGLMPEDVAAAMAKRSREGYS 127 + + E + + + ++ A + E + ++ + + Sbjct: 68 YVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLD 127 Query: 128 SLPKEKLEDIALVDGGLVSMQLMLAAKARGYDTVPMGGYDAAALKELFRIS-DRYIPVML 186 + G L A DT+ A K + + ++ Sbjct: 128 LYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVM 187 Query: 187 IALGKAAVEGRPTNRLPLEAVVHW 210 L A P Sbjct: 188 RYLHLADKHDLPRIVTIQNPYSLL 211 >BS_yodC [C] COG0778 Nitroreductase Length = 346 Score = 159 bits (401), Expect = 4e-39, Method: Compositional matrix adjust. Identities = 8/197 (4%), Positives = 27/197 (13%), Gaps = 2/197 (1%) Query: 14 VIRERRSVRKYDSSWKISDXXXXXXXXXXXXXPSSSNLQPWRFIVITDQEIKAKLLPIAH 73 + + + + + + + + Sbjct: 7 PHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTE 66 Query: 74 NQAQTTESSAMIAVLGDTEAYSNAEKIYGHASATGLMPEDVAAAMAKRSREGYSSLPKEK 133 + + + D + Sbjct: 67 TYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRL--QTD 124 Query: 134 LEDIALVDGGLVSMQLMLAAKARGYDTVPMGGYDAAALKELFRISDRYIPVMLIALGKAA 193 D+ V D+ P I + ++ A Sbjct: 125 YLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAF 184 Query: 194 VEGRPTNRLPLEAVVHW 210 R + + Sbjct: 185 GVMRYLHLADKHDLPRI 201 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.129 0.363 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 13,624,407 Number of extensions: 495593 Number of successful extensions: 10836 Number of sequences better than 1.0e-05: 42 Number of HSP's gapped: 10828 Number of HSP's successfully gapped: 42 Length of query: 215 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 113 Effective length of database: 26,614,363 Effective search space: 3007423019 Effective search space used: 3007423019 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 113 (48.1 bits)