BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0391.0010.p (373 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM0382 [GEPR] COG0477 Permeases of the major facilitator superf... 170 3e-42 BS_yceE [T] COG2310 Uncharacterized proteins involved in stress ... 165 1e-40 L129379 [GEPR] COG0477 Permeases of the major facilitator superf... 155 8e-38 SPy0501 [GEPR] COG0477 Permeases of the major facilitator superf... 154 2e-37 SP0972 [GEPR] COG0477 Permeases of the major facilitator superfa... 144 3e-34 >STM0382 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 403 Score = 170 bits (431), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 116/348 (33%), Positives = 183/348 (52%), Gaps = 9/348 (2%) Query: 4 SWKRNLIVLWVGVFFCSTAYXXXXXXXXXXXXDQLGVKNH--LEIWSGVSFGITFLASAL 61 SWK NLI +W G FF A QLGV +H L +WSG++F ITFL SA+ Sbjct: 3 SWKVNLISVWFGCFFTGLAISQILPFLPLYI-SQLGVSSHEALSMWSGLTFSITFLISAI 61 Query: 62 ISPYWGSLADKYGRKPMLIRSGFSLAALYLINFFVHDPYVFIIVRIFQGLLAGFVPAAIA 121 +SP WGSLAD+ GRK ML+R+ +A L+ F + ++R GL +G++P A+A Sbjct: 62 VSPMWGSLADRKGRKLMLLRASLGMAIAILLQAFATHVWQLFLLRGIMGLTSGYIPNAMA 121 Query: 122 MVATNTPEEKTGYALSIMSTAGATGSIIGPLIGGVVSFYSSNRSAFLFSAVIVLVSALIA 181 +VA+ P E++G+ALS +STA +G I GPL+GG V+ + R+ FL +A++++VS L+ Sbjct: 122 LVASQVPRERSGWALSTLSTAQISGVIGGPLMGGFVADHIGLRAVFLITAMLLVVSFLVT 181 Query: 182 TFFAKEENYDRSAPRSHVRDDIREAAANPAFMTLLMLAGLATFSVMI----LEPLLPIYL 237 F KE R + R + A+ + L++ T + + + P+L +++ Sbjct: 182 LFLIKEG--VRPVIKKSERLSGKAVFASLPYPALVISLFFTTMVIQLCNGSISPILALFI 239 Query: 238 LDMGISRNSASLSSGIVFSAVGIATVIMAPQWGRLGSRKXXXXXXXXXXXXXXXXXXXQY 297 M N+ + SG++ S GI+ +I AP+ G+LG R Sbjct: 240 KSMMPDSNNIAFLSGLIASVPGISALISAPRLGKLGDRIGTERILMATLICAVLLFFAMS 299 Query: 298 FVSGYVEFAILRFAYGLFYAGVLPSVNAMIVQVIDPGFRGRAFGLNQA 345 +V+ + +LRF G +LP+V ++V+ GR FG NQ+ Sbjct: 300 WVTTPFQLGLLRFLLGFADGAMLPAVQTLLVKYSSDQITGRIFGYNQS 347 >BS_yceE [T] COG2310 Uncharacterized proteins involved in stress response, homologs of TerZ and putative cAMP-binding protein CABP1 Length = 408 Score = 165 bits (417), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 115/351 (32%), Positives = 184/351 (52%), Gaps = 16/351 (4%) Query: 4 SWKRNLIVLWVGVFFCSTAYXXXXXXXXXXXXDQLGVKNH--LEIWSGVSFGITFLASAL 61 +WKRNLIV W+G F A+ +QLGV H L +WSG+ F ITFL SA+ Sbjct: 11 NWKRNLIVAWLGCFLTGAAFSLVMPFLPLYV-EQLGVTGHSALNMWSGIVFSITFLFSAI 69 Query: 62 ISPYWGSLADKYGRKPMLIRSGFSLAALYLINFFVHDPYVFIIVRIFQGLLAGFVPAAIA 121 SP+WG LAD+ GRK ML+RS + + ++ + + F+I+R GLL GFVP A A Sbjct: 70 ASPFWGGLADRKGRKLMLLRSALGMGIVMVLMGLAQNIWQFLILRALLGLLGGFVPNANA 129 Query: 122 MVATNTPEEKTGYALSIMSTAGATGSIIGPLIGGVVSFYSSNRSAFLFSAVIVLVSALIA 181 ++AT P K+G+AL +ST G +G+++GP+ GG+++ R F +A VL+ Sbjct: 130 LIATQVPRNKSGWALGTLSTGGVSGALLGPMAGGLLADSYGLRPVFFITAS-VLILCFFV 188 Query: 182 TFFAKEENYDRSAPRS--HVRDDIREAAANPA-----FMTLLMLAGLATFSVMILEPLLP 234 T F E + + + H+R+ + + NP F+T L++ +AT S+ P+L Sbjct: 189 TLFCIREKFQPVSKKEMLHMREVV-TSLKNPKLVLSLFVTTLIIQ-VATGSI---APILT 243 Query: 235 IYLLDMGISRNSASLSSGIVFSAVGIATVIMAPQWGRLGSRKXXXXXXXXXXXXXXXXXX 294 +Y+ ++ + ++ + SG++ S G+A ++ AP+ G+LG R Sbjct: 244 LYVRELAGNVSNVAFISGMIASVPGVAALLSAPRLGKLGDRIGPEKILITALIFSVLLLI 303 Query: 295 XQYFVSGYVEFAILRFAYGLFYAGVLPSVNAMIVQVIDPGFRGRAFGLNQA 345 +V ++ ILRF G +LP+V ++V GR F NQ+ Sbjct: 304 PMSYVQTPLQLGILRFLLGAADGALLPAVQTLLVYNSSNQIAGRIFSYNQS 354 >L129379 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 398 Score = 155 bits (393), Expect = 8e-38, Method: Compositional matrix adjust. Identities = 106/352 (30%), Positives = 173/352 (49%), Gaps = 4/352 (1%) Query: 1 MRFSWKRNLIVLWVGVFFCSTAYXXXXXXXXXXXXDQLG--VKNHLEIWSGVSFGITFLA 58 M+ SW+RNLIVLW G F LG K L IWSG+ F TF+ Sbjct: 1 MKESWQRNLIVLWFGTFMTGIG-SSLIAPFISLYISTLGHYSKTELNIWSGLVFSSTFIV 59 Query: 59 SALISPYWGSLADKYGRKPMLIRSGFSLAALYLINFFVHDPYVFIIVRIFQGLLAGFVPA 118 A++SP WG LAD+ GRK ML+R+ +A + FV + +I+R+ G +GFV Sbjct: 60 LAIVSPLWGRLADQKGRKLMLLRASLGMAISISLMAFVTAAWQLLILRMLLGAFSGFVSN 119 Query: 119 AIAMVATNTPEEKTGYALSIMSTAGATGSIIGPLIGGVVSFYSSNRSAFLFSAVIVLVSA 178 ++A++A++ P+EK+G LS+++T G++IGP+IGG++ + R F + +I+ + Sbjct: 120 SMALMASSAPKEKSGAVLSLLTTGSVAGTLIGPIIGGILVNITGYRRVFSVTGIIMFLVF 179 Query: 179 LIATFFAKEENYDRSAPRSHVRDDIREAAANPAFMTLLMLAGLAT-FSVMILEPLLPIYL 237 +A FF KE + E +PA + + A L T + + P+L +Y+ Sbjct: 180 FLALFFVKEIFKPVEKKNMLSYKQVWEVVTHPAIIWGMFFATLITQMTNQSINPVLSLYV 239 Query: 238 LDMGISRNSASLSSGIVFSAVGIATVIMAPQWGRLGSRKXXXXXXXXXXXXXXXXXXXQY 297 ++ + + + +GIV +A GI T++ AP GRLG + Sbjct: 240 QELMHGQGNITFMAGIVAAAPGIVTLVAAPFLGRLGDKVGQRKILGFGLFFSLIVFSITA 299 Query: 298 FVSGYVEFAILRFAYGLFYAGVLPSVNAMIVQVIDPGFRGRAFGLNQAATQL 349 + ++R G+ A +LPSV A++ + GR F NQ+A + Sbjct: 300 MTTNVWFLIVMRLLVGVSDAAILPSVQAILAKESPQAITGRIFSYNQSAQSI 351 >SPy0501 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 397 Score = 154 bits (389), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 101/350 (28%), Positives = 179/350 (51%), Gaps = 8/350 (2%) Query: 4 SWKRNLIVLWVGVFFCSTAYXXXXXXXXXXXXDQLGVKNHL-EIWSGVSFGITFLASALI 62 +W++NL V W+G FF ++ + LG L E ++G++ +T LASAL Sbjct: 2 NWRQNLKVAWLGNFFTGASFSLVMPFMALYV-ENLGTPTELVEYYAGLAVAVTALASALF 60 Query: 63 SPYWGSLADKYGRKPMLIRSGFSLAALYLINFFVHDPYVF--IIVRIFQGLLAGFVPAAI 120 +P WG LAD+YGRKPM++R+ F + + + P VF +I+R+ G+ AG+VP A Sbjct: 61 APVWGKLADRYGRKPMMLRASFVMT--FTMGGLAIIPNVFWLLILRLLTGVSAGYVPNAT 118 Query: 121 AMVATNTPEEKTGYALSIMSTAGATGSIIGPLIGGVVSFYSSNRSAFLFSAVIVLVSALI 180 A++A+ P+E++GYAL ++T G++IGPL+GG+++ R FL VI+ + +L+ Sbjct: 119 ALIASQAPKEESGYALGTLATGVTAGALIGPLLGGILAELLGIRQVFLLVGVILFLCSLM 178 Query: 181 ATFFAKEE-NYDRSAPRSHVRDDIREAAANPAFMTLLMLAGLATFSVMILEPLLPIYLLD 239 + KEE R + +++ + + L + + + S + P+L +Y+ Sbjct: 179 TAVYVKEEFKPVRRFEMIPTKVILKQVKSPQIMLGLFVTSMIIQISAQSVAPILSLYIRH 238 Query: 240 MGISRNSASLSSGIVFSAVGIATVIMAPQWGRLGSRKXXXXXXXXXXXXXXXXXXXQYFV 299 +G + N +SG+V SA+G +++ + G+LG R Sbjct: 239 LGQTHN-LMFTSGLVVSAMGFSSLFSSSYLGKLGDRFGNHRLLLAALCYSFIMYFSSALA 297 Query: 300 SGYVEFAILRFAYGLFYAGVLPSVNAMIVQVIDPGFRGRAFGLNQAATQL 349 + +LRFAYG ++PS+N+++ ++ R F NQ + L Sbjct: 298 QTSFQLGVLRFAYGFGVGALMPSINSLLTKLTPKEGISRVFAYNQMFSNL 347 >SP0972 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 399 Score = 144 bits (362), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 97/345 (28%), Positives = 172/345 (49%), Gaps = 4/345 (1%) Query: 2 RFSWKRNLIVLWVGVFFCSTAYXXXXXXXXXXXXDQLGV-KNHLEIWSGVSFGITFLASA 60 +WK NL + W G F + A + LGV + ++G++ ++ +++A Sbjct: 3 EINWKDNLRIAWFGNFL-TGASISLVVPFMPIFVENLGVGSQQVAFYAGLAISVSAISAA 61 Query: 61 LISPYWGSLADKYGRKPMLIRSGFSLAALYLINFFVHDPYVFIIVRIFQGLLAGFVPAAI 120 L SP WG LADKYGRKPM+IR+G ++ FV + Y I +R+ G+ AGFVP A Sbjct: 62 LFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYWLIFLRLLNGVFAGFVPNAT 121 Query: 121 AMVATNTPEEKTGYALSIMSTAGATGSIIGPLIGGVVSFYSSNRSAFLFSAVIVLVSALI 180 A++A+ P+EK+G AL +ST G++ GP IGG ++ R+ FL + ++A++ Sbjct: 122 ALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAELFGIRTVFLLVGSFLFLAAIL 181 Query: 181 ATFFAKEENYDRSAPRSHVRDDIREAAANP-AFMTLLMLAGLATFSVMILEPLLPIYLLD 239 F KE+ + ++ ++ + P + L + + + FS + P+L +Y+ D Sbjct: 182 TICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLFLTSFVIQFSAQSIGPILALYVRD 241 Query: 240 MGISRNSASLSSGIVFSAVGIATVIMAPQWGRLGSRKXXXXXXXXXXXXXXXXXXXQYFV 299 +G + N SG++ S++G ++++ A G+LG + Sbjct: 242 LGQTEN-LLFVSGLIVSSMGFSSMMSAGVMGKLGDKVGNHRLLVVAQFYSVIIYLLCANA 300 Query: 300 SGYVEFAILRFAYGLFYAGVLPSVNAMIVQVIDPGFRGRAFGLNQ 344 S ++ + RF +GL ++P VNA++ ++ R F NQ Sbjct: 301 SSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPKAGISRVFAFNQ 345 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.327 0.140 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,252,246 Number of extensions: 753609 Number of successful extensions: 3114 Number of sequences better than 1.0e-05: 59 Number of HSP's gapped: 3138 Number of HSP's successfully gapped: 65 Length of query: 373 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 265 Effective length of database: 25,922,359 Effective search space: 6869425135 Effective search space used: 6869425135 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 116 (49.3 bits)