BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0391.0008.p (443 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1803 [P] COG0038 Chloride channel protein EriC 333 4e-91 L105113 [P] COG0038 Chloride channel protein EriC 216 5e-56 SPy0723 [P] COG0038 Chloride channel protein EriC 184 2e-46 mll2953_1 [P] COG0038 Chloride channel protein EriC 69 2e-11 mll1477_1 [P] COG0038 Chloride channel protein EriC 59 2e-08 >RSc1803 [P] COG0038 Chloride channel protein EriC Length = 431 Score = 333 bits (853), Expect = 4e-91, Method: Compositional matrix adjust. Identities = 188/416 (45%), Positives = 250/416 (60%), Gaps = 3/416 (0%) Query: 25 IKWIVLGGCVGILSGSASAIFLKSLDAVTEVRLNHAWXXXXXXXXXXXVSFLYMRYGKNS 84 ++W+ L G+L+G SA L +LD T R H W V LY G+ Sbjct: 6 LRWLFLASLTGVLAGLGSAALLLALDWATLTRTAHPWLLWLLPLAGLAVGLLYHYTGRAV 65 Query: 85 AKGNNLILEQIQQGNETIPMRMAPLVLFGTLVTHLFGGSAGREGTAVQMGGSLAEGFGRL 144 GNNL++++I +P RMAPL+L GT+VTHLFGGSAGREGTAVQMGGS A+G RL Sbjct: 66 EGGNNLLIDEIHDPKRVVPKRMAPLILLGTVVTHLFGGSAGREGTAVQMGGSFADGLTRL 125 Query: 145 IRINSLDRQILLMCXXXXXXXXXXXTPLAGTLFGLEVVAIGLISHKALLPCFTASFIGDI 204 R+++ R+ILLM TPLAG +FGLEV+AIG + + A+LPCF A+ +GD+ Sbjct: 126 FRLDNEARRILLMSGISAGFASVFGTPLAGAVFGLEVLAIGRLRYDAILPCFVAAIVGDL 185 Query: 205 VATRLWKVQHIHYHVELFPPLTAIILLKVILASVLFGLASILFSELTHYLKKTFTRLVKN 264 V L V H Y + P LT + + V++A ++FGLA + F+ELTH L + + + Sbjct: 186 VPP-LLGVHHTPYAIPFVPHLTPLAIGAVVVAGIVFGLAGMTFAELTHRLGRWLKKRIPF 244 Query: 265 PMLKSALGGFIIIALVYIAGTRDYLGLGIPLIQDSFQTDVAPFAFLWKLIFTSFTLGTGF 324 L+ LGG ++ A GT YLGLGIP+I D+F + + F K FT TL +GF Sbjct: 245 GPLRPVLGGCVVAAAATALGTDKYLGLGIPVIVDAFHQPLPAYDFAGKAAFTIVTLASGF 304 Query: 325 QGGEVTPLFAIGATLGNSLASFLHLHAPFLASLGFIAVFCGATNTPVACFLMGIELFGSG 384 +GGEVTPLF IGATLGN+L L L LA LGF+AVF GA NTP+A +M IELFG Sbjct: 305 KGGEVTPLFYIGATLGNALGYVLPLPFALLAGLGFVAVFAGAANTPIASTIMAIELFGPE 364 Query: 385 GAVYMFIACLISYLFSGHSGIYTSQQIGISKSEWVRIPEGTTLGTAKQLRTTKKTS 440 + IAC++SYLFSGH+GIY +Q++G +K +PEG L R + Sbjct: 365 VGTFAGIACVVSYLFSGHTGIYPAQRVGHAKR--ASVPEGLRLSEVPAFRQASTAA 418 >L105113 [P] COG0038 Chloride channel protein EriC Length = 403 Score = 216 bits (551), Expect = 5e-56, Method: Compositional matrix adjust. Identities = 133/385 (34%), Positives = 212/385 (55%), Gaps = 10/385 (2%) Query: 28 IVLGGCVGILSGSASAIFLKSLDAVTEVRLNHA-WXXXXXXXXXXXVSFLYMRYGKNSAK 86 +++GG +GI+ AIF + L +TE R +HA + + ++Y +GK+S K Sbjct: 20 VIIGGLIGIVD----AIFGRGLIYITEFRDSHALYLIPFLTLAGLLIVYIYKNFGKDSQK 75 Query: 87 GNNLILEQIQQGNETIPMRMAPLVLFGTLVTHLFGGSAGREGTAVQMGGSLAEGFGRLIR 146 G LI E G E IP R+ PL++F T ++HLFG S GREG AVQ+GG + G+ + Sbjct: 76 GMGLIFEAGNHGREDIPKRLVPLIVFSTWLSHLFGASVGREGVAVQIGGVIGHAIGKKLS 135 Query: 147 INSLDRQILLMCXXXXXXXXXXXTPLAGTLFGLEVVAIGLISHKALLPCFTASFIGDIVA 206 ++ILL+ TP+A T F +E++ +G I ++AL+P S++ + Sbjct: 136 AKE-AKKILLITGMAAGFAGLFQTPIAATFFAIEILMLGKIEYRALIPALVGSYVASWTS 194 Query: 207 TRLWKVQHIHYHVELFPPLTAIILLKVILASVLFGLASILFSELTHYLKKTFTRLVKNPM 266 + L ++ + + + ++LLK+ + +V FGL F+E ++K T + + NP Sbjct: 195 SSL-GLEKFSFAINTNIHIDPLVLLKLAVIAVCFGLVGRFFAESLAFMKATVAKRIVNPY 253 Query: 267 LKSALGGFII-IALVYIAGTRDYLGLGIPLIQDSFQT-DVAPFAFLWKLIFTSFTLGTGF 324 + L G +I I L+ I R Y GLG LI SF + + ++ KLIFT ++ GF Sbjct: 254 YRIILMGIVISIGLLVIHLDR-YAGLGTNLISLSFNGGHINGYDWILKLIFTVLSISAGF 312 Query: 325 QGGEVTPLFAIGATLGNSLASFLHLHAPFLASLGFIAVFCGATNTPVACFLMGIELFGSG 384 QGGEVTPLFAIG+TLG +LA L F+A+ G++++F ATN+ + E+FG G Sbjct: 313 QGGEVTPLFAIGSTLGAALAMLFGLPVEFVAAAGYVSIFSAATNSYFGPIFIAAEVFGFG 372 Query: 385 GAVYMFIACLISYLFSGHSGIYTSQ 409 Y+ I+Y+ +G+S IY + Sbjct: 373 SVQYILPIMTIAYVLNGNSSIYAQE 397 >SPy0723 [P] COG0038 Chloride channel protein EriC Length = 420 Score = 184 bits (468), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 131/384 (34%), Positives = 199/384 (51%), Gaps = 8/384 (2%) Query: 30 LGGCVGILSGSASAIFLKSLDAVTEVRLNHAWXXXXXXXXXXXV-SFLYMRYGKNSAKGN 88 +GG VG++ IF K L ++E R +H + V FLY + GK +G Sbjct: 26 IGGAVGVVD----VIFGKGLLFLSEYRDHHLFLLLPFLALAGLVIVFLYDKLGKEVRQGM 81 Query: 89 NLILEQIQQGNETIPMRMAPLVLFGTLVTHLFGGSAGREGTAVQMGGSLAEGFGRLIRIN 148 L+ + IP + PL+LF T VTHLFG SAGREG AVQ+G +++ R + Sbjct: 82 GLVFQVGHGQKNQIPPMLIPLILFSTWVTHLFGASAGREGVAVQIGATISHYCRRFVTSQ 141 Query: 149 SLDRQILLMCXXXXXXXXXXXTPLAGTLFGLEVVAIGLISHKALLPCFTASFIGDIVATR 208 R +L+M TP+A +F LEV+ +G + + ALLP A+++ + Sbjct: 142 EAARHLLIMGMAAGFAGLFQ-TPIAAVVFALEVLLVGTLRYSALLPSLVAAYVASWTSHA 200 Query: 209 LWKVQHIHYHVELFPPLTAIILLKVILASVLFGLASILFSELTHYLKKTFTRLVKNPMLK 268 L ++ +E +T + L+K+I ++FGL F+ L + K ++ + NP + Sbjct: 201 L-GLEKFTIVLEETLTITPLTLVKLIGLGLIFGLVGNSFAYLLGWFKPYLSQKLPNPYFR 259 Query: 269 SALGGFIIIALVYIAGTRDYLGLGIPLIQDSFQ-TDVAPFAFLWKLIFTSFTLGTGFQGG 327 A G ++ + I Y GLG LI +F + + +L K+I T +L GFQGG Sbjct: 260 IAFIGALLSICLMIGHVGRYSGLGTNLIAAAFSGQTILTYDWLLKMIVTVISLSAGFQGG 319 Query: 328 EVTPLFAIGATLGNSLASFLHLHAPFLASLGFIAVFCGATNTPVACFLMGIELFGSGGAV 387 EVTPLFAIGA+LG LA +L L +A+LG+ VF ATNT A +GIE+FG A+ Sbjct: 320 EVTPLFAIGASLGIVLAPYLGLPVLLVAALGYTTVFGSATNTFWAPIFIGIEVFGPENAL 379 Query: 388 YMFIACLISYLFSGHSGIYTSQQI 411 F+ +Y+ S IY+ Q++ Sbjct: 380 AYFVTSAAAYMVSHRHSIYSYQKV 403 >mll2953_1 [P] COG0038 Chloride channel protein EriC Length = 461 Score = 68.6 bits (166), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 76/279 (27%), Positives = 122/279 (43%), Gaps = 19/279 (6%) Query: 114 TLVTHLFGGSAGREGTAVQMGGSLAEGFGRLIRINSLDRQILLMCXXXXXXXXXXXTPLA 173 T+++ FG S G E Q+G LA R+ R+ D ++L+ C PL Sbjct: 133 TMISSGFGASVGLEAGYTQVGSGLASRLARIFRLRRNDVRVLVGCGAAGAIAAAFDAPLT 192 Query: 174 GTLFGLEVVAIGLISHKALLPCFTASFIGDIVATRLWKVQHIHYHVEL--FPPLTAIILL 231 G +G E+V IG+ S + P TA+ + A V + +EL P LT + Sbjct: 193 GAFYGFELV-IGIYSVANVAPVMTAAISASLTAEMFGGVP---FPLELSGLPQLTPSQYV 248 Query: 232 KVILASVLFGLASILFSELTHYLKKTFTRLVKNPMLKSALGGFIIIALVYIAGTRDYLGL 291 +L +L G ASI +L +++ F RL + L+ +GG I+ L I T L Sbjct: 249 PFLLLGLLGGAASIAIMQLVTLIERGFNRLSVDASLRPVIGGVIVGLLGLI--TPQVLSS 306 Query: 292 GIPLIQDSFQTD----VAPFAFLWKLIFTSFTLGTGFQGGEVTPLFAIGATLGNSLASFL 347 G + F + V F+ KL ++ +LG+GF+GG +GA LG + A + Sbjct: 307 GHGALHREFAMNYGLTVVASVFVLKLAASAISLGSGFRGGLFFASLFLGALLGKAFAGVM 366 Query: 348 HLHAP-------FLASLGFIAVFCGATNTPVACFLMGIE 379 L +P A +G ++ G P+ + +E Sbjct: 367 ALISPATGIDPSVAAVVGMTSLAVGVVGGPLTMTFLALE 405 >mll1477_1 [P] COG0038 Chloride channel protein EriC Length = 460 Score = 58.9 bits (141), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 76/324 (23%), Positives = 132/324 (40%), Gaps = 29/324 (8%) Query: 76 LYMRYGKNSAKGNNL--ILEQIQQGNETIPMRMAPLVLFGTLVTHLFGGSAGREGTAVQM 133 L RYG +G+ + +E I G + ++A L + ++ GG G EG + Sbjct: 98 LMARYGSEKIRGHGIPEAIEAILLGRSRLGAKVAILKPLSSAISIGSGGPFGAEGPIIMT 157 Query: 134 GGSLAEGFGRLIRINSLDRQILLMCXXXXXXXXXXXTPLAGTLFGLEVVAIGLISHKALL 193 GG++ +++ ++ +R+ LL+ TP+A + +E++ + ++ + Sbjct: 158 GGAIGSLIAKMLPVSDNERKTLLVAGAAAGMTTVFGTPIAAIMLAVELLLFEW-TPRSFI 216 Query: 194 PCFTASFIGDIVATRLWKVQHIHYHVELFP-----PLTAIILLKVILASVLFGLASILFS 248 P A+ I ++ T L H +FP ++ L +L + GL S L + Sbjct: 217 PVAVAAIIAEVERTAL------HLPGPIFPFQGGMAVSFTGLGGWVLVGICAGLLSGLLT 270 Query: 249 ELTHYLKKTFTRLVKN----PMLKSALGGFIIIALVYIAGTRDYLGLGIPLIQDSFQ-TD 303 ++ + + F +L + PM+ + + LG+G I D Sbjct: 271 QMVYACEDAFQKLPIHWMWWPMIGGLV------VGIGGLIEPRALGVGYDNIADMLDGRT 324 Query: 304 VAPFAFLW---KLIFTSFTLGTGFQGGEVTPLFAIGATLGNSLASFLHLHAP-FLASLGF 359 VA A L K I S LG+G GG + PL +G +G LA L P F A L Sbjct: 325 VATAALLLLVIKAIIWSVALGSGTSGGVLAPLLIMGGAMGAVLAGILPAADPGFWALLAM 384 Query: 360 IAVFCGATNTPVACFLMGIELFGS 383 A G P+ +EL G+ Sbjct: 385 SATMGGTMRAPLTATFFAVELTGN 408 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.327 0.142 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 29,711,030 Number of extensions: 1164770 Number of successful extensions: 4994 Number of sequences better than 1.0e-05: 30 Number of HSP's gapped: 4960 Number of HSP's successfully gapped: 30 Length of query: 443 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 333 Effective length of database: 25,691,691 Effective search space: 8555333103 Effective search space used: 8555333103 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 117 (49.7 bits)