BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0389.0005.p (249 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value FN0729 [G] COG0588 Phosphoglycerate mutase 1 262 3e-70 ML0011 [O] COG0652 Peptidyl-prolyl cis-trans isomerase (rotamase... 236 2e-62 YKL152c [G] COG0588 Phosphoglycerate mutase 1 221 8e-58 YOL056w [G] COG0588 Phosphoglycerate mutase 1 133 2e-31 YDL021w [G] COG0588 Phosphoglycerate mutase 1 132 5e-31 >FN0729 [G] COG0588 Phosphoglycerate mutase 1 Length = 346 Score = 262 bits (670), Expect = 3e-70, Method: Compositional matrix adjust. Identities = 20/222 (9%), Positives = 45/222 (20%) Query: 3 EIVLIRHGESEYNRQNLFTGWSDPDLTEKGVEEAKAAGKLLREAGYTFDLAFASVLKRSI 62 + I H E + L T +E + + Sbjct: 2 QYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPET 61 Query: 63 KTLNYVLDEMDLLWIPVQKSWKLNERHYGALQGLSKSETAVKYGEEQLHIWRRSLSVRPP 122 + L L ++ + + G + K + + + S++ Sbjct: 62 QGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRL 121 Query: 123 MLDPDDPRYARNDIRYKEVRPGDIPRGESLEDTVHRVGDFWGNRIVPLIRKKERVLISAH 182 D D R V + K + +S Sbjct: 122 QTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNE 181 Query: 183 GNTLRALIKFMEDIDEKALLDLNVPTGVPLVYKLDDDVKPIS 224 + D + + L + + +S Sbjct: 182 TAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVS 223 >ML0011 [O] COG0652 Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family Length = 252 Score = 236 bits (603), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 13/229 (5%), Positives = 40/229 (17%) Query: 1 MYEIVLIRHGESEYNRQNLFTGWSDPDLTEKGVEEAKAAGKLLREAGYTFDLAFASVLKR 60 M + + R E A A+ + Sbjct: 1 MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITG 60 Query: 61 SIKTLNYVLDEMDLLWIPVQKSWKLNERHYGALQGLSKSETAVKYGEEQLHIWRRSLSVR 120 + + + L + L + ++++ + ++ +S+ Sbjct: 61 IDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLL 120 Query: 121 PPMLDPDDPRYARNDIRYKEVRPGDIPRGESLEDTVHRVGDFWGNRIVPLIRKKERVLIS 180 P + + + + E + Sbjct: 121 NPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWR 180 Query: 181 AHGNTLRALIKFMEDIDEKALLDLNVPTGVPLVYKLDDDVKPISRFYLG 229 + + D L + +L Sbjct: 181 TTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQY 229 >YKL152c [G] COG0588 Phosphoglycerate mutase 1 Length = 346 Score = 221 bits (563), Expect = 8e-58, Method: Compositional matrix adjust. Identities = 8/246 (3%), Positives = 40/246 (16%) Query: 1 MYEIVLIRHGESEYNRQNLFTGWSDPDLTEKGVEEAKAAGKLLREAGYTFDLAFASVLKR 60 M + + + + + A ++ Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 61 SIKTLNYVLDEMDLLWIPVQKSWKLNERHYGALQGLSKSETAVKYGEEQLHIWRRSLSVR 120 + + +K ++ + + + R Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 121 PPMLDPDDPRYARNDIRYKEVRPGDIPRGESLEDTVHRVGDFWGNRIVPLIRKKERVLIS 180 D + +S + + + + Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 181 AHGNTLRALIKFMEDIDEKALLDLNVPTGVPLVYKLDDDVKPISRFYLGEPEEVQEKARE 240 + + + D ++ + P + + + Sbjct: 181 ETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGT 240 Query: 241 VANQSK 246 + + Sbjct: 241 LTGKYL 246 >YOL056w [G] COG0588 Phosphoglycerate mutase 1 Length = 346 Score = 133 bits (335), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 12/292 (4%), Positives = 37/292 (12%), Gaps = 50/292 (17%) Query: 2 YEIVLIRHGESEYNRQNLFTGWSDPDLTEKGVEEAKAAGKLLREAGYTFDLA-----FAS 56 L + A+ + Y Sbjct: 7 PHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTE 66 Query: 57 VLKRSIKTLNYVLDEMDLLW-----------------------IPVQKSWKLNERHYGAL 93 + + +++ + I L L Sbjct: 67 TYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYL 126 Query: 94 QGLSKSETAVKYGEEQLHIWRRSLSVRPP-------MLDPDDPRYARNDIRYKE----VR 142 + + S L I Sbjct: 127 DLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGV 186 Query: 143 PGD-------IPRGESLEDTVHRVGDFWGNRIVPLIRK--KERVLISAHGNTLRALIKFM 193 + + + + + + +L + K++ Sbjct: 187 MRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYL 246 Query: 194 EDIDEKALLDLNVPTGVPLVYKLD-DDVKPISRFYLGEPEEVQEKAREVANQ 244 + + V + + Sbjct: 247 NGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIAR-RHGLDPAQMALAFVR 297 >YDL021w [G] COG0588 Phosphoglycerate mutase 1 Length = 346 Score = 132 bits (332), Expect = 5e-31, Method: Compositional matrix adjust. Identities = 18/295 (6%), Positives = 40/295 (13%), Gaps = 55/295 (18%) Query: 4 IVLIRHGESEYNRQNLFTGWSDPDLTEKGVEEAKAAGKLLREAGYTFDL-----AFASVL 58 V + + + L + Sbjct: 12 EVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGN 71 Query: 59 KRSIKTLNYVLDEMDL--------------------------LWIPVQKSWKLNERHYGA 92 + L L +++ Y Sbjct: 72 WLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQV 131 Query: 93 LQGLSKSETAVKYGEEQLHIWRRSLSVRPPMLDPDDPRYARND----------------I 136 + K G + + R + Sbjct: 132 HWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLH 191 Query: 137 RYK-EVRPGDI--PRGESLEDTVHRVGDFWGNRIVPLIRK--KERVLISAHGNTLRALIK 191 P + SL + VG ++ + + G L Sbjct: 192 LADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKP 251 Query: 192 FMEDIDEKALLDLNVPTGVPLVYKLDDD--VKPISRFYLGEPEEVQEKAREVANQ 244 + D + + VA+ Sbjct: 252 AGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQ-MALAFVRRQPFVAST 305 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.137 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,614,178 Number of extensions: 816025 Number of successful extensions: 1884 Number of sequences better than 1.0e-05: 38 Number of HSP's gapped: 1888 Number of HSP's successfully gapped: 38 Length of query: 249 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 145 Effective length of database: 26,383,695 Effective search space: 3825635775 Effective search space used: 3825635775 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 114 (48.5 bits)