BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0386.0036.p (508 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yunI [TQ] COG2508 Regulator of polyketide synthase expression 173 7e-43 BH0757 [TQ] COG2508 Regulator of polyketide synthase expression 125 2e-28 BH1735 [TQ] COG2508 Regulator of polyketide synthase expression 112 1e-24 BH1994_2 [TQ] COG2508 Regulator of polyketide synthase expression 94 6e-19 CAC1426 [TQ] COG2508 Regulator of polyketide synthase expression 87 8e-17 >BS_yunI [TQ] COG2508 Regulator of polyketide synthase expression Length = 531 Score = 173 bits (438), Expect = 7e-43, Method: Compositional matrix adjust. Identities = 133/530 (25%), Positives = 242/530 (45%), Gaps = 61/530 (11%) Query: 6 ITLRELMQLPILSKATVISGMQGLDRVVRFVDVMEVPDLKGWISEGVMLLTTAYSIRHDP 65 + + ++M++P A +I+G G +R V+ V++M+ PD+ ++ + +L+TTAY ++ P Sbjct: 1 MNILDVMKIPAFENANLIAGKAGGEREVQHVNMMDAPDIVDFLHKNELLVTTAYHLKDHP 60 Query: 66 SLLTELIYTLDQLGAAALAIKPARFLKEIPQGAIEASNDCGLPIVEIPPEIPYTDITQPV 125 L+ELI + + G A L IK R+L++IP+ IE ++ PI+E+P I DI Sbjct: 61 HQLSELIRQMAKRGCAGLGIKTKRYLEDIPKEIIELADSYAFPIIELPEHIRLGDIVNAT 120 Query: 126 MELLLGRQAMLLRRAEEVYRTLTTMVLENSGIQAVSDNVADFLKAPVALLDTERNIIVSS 185 + +L ++ L++A ++ T ++ G+Q++ V+D L+ PV LLD ++ +S Sbjct: 121 LSHILDMRSNELQQAIYAHKKFTNHIMSGKGLQSLLKKVSDILQLPVLLLDQHAKMLSAS 180 Query: 186 PSQFDWSLAERPLSWNIHV-------------DRRTVARLLVDKEQ-------LDDMEEV 225 S+ L ++ D++T + L + + + DM + Sbjct: 181 ---HQISVETEKLKGTLNTVSGPFFTCFSTISDQKTYSVLPIYNHEKNCGYLLIPDMVQA 237 Query: 226 G-------IEQARLVFALELMRKKVAEDTEFRLRGNFIDELLTPPLPSRHEVERRARQLG 278 G IEQA V + EL+++ + R R F + + S E++ RA++ Sbjct: 238 GDKGLILTIEQAANVISFELLKENALKQFSRRARNEFFNNFIERTFSSDDEIKNRAKEFK 297 Query: 279 MNPEHKWEVAVIEGETAPNEETVN------------RLLEQEARKRGVTPHAEFRSNRAV 326 + + K+ I G+ N+E+++ LE E PH + N + Sbjct: 298 LRWDQKY--MCIAGKLDRNDESISFTENQLASDSVFEFLEGELSAFPFPPHFFMKGNVGI 355 Query: 327 LFLPTPEGRKFGVAGDKGTSWAVTLEGWLSD-KAEGLGGYRTGV-----GTPEYLWDIQT 380 + + A D + ++ +L + + ++ V + L D+ Sbjct: 356 ILIE---------ATDSWSEMHASVISFLEQFQTQVSAQFKRTVSFGISNICQKLIDVPD 406 Query: 381 SYNEARKALSVAKRLGQGTGGVTRYEEIEVYHLLEGLSGEGFAALFERKLGKLRQYDQEH 440 ++ EA AL + L + T + Y +V LL L E + L L + QE Sbjct: 407 AFTEASDALQ-SGHLSRSTAFIQVYHAKDVPELLRLLPVEDLKKFYNSTLQSLAEKQQE- 464 Query: 441 DSNMLLTFYHYLECRGSLIETANSLYIHRNSVKYRLERIRDITGFDLNDP 490 D ++L T YLE + ETA LY+HRN+V YRLE+ ++ G L DP Sbjct: 465 DQSLLHTLSVYLETHCQISETAKRLYVHRNTVIYRLEKCEELLGKSLKDP 514 >BH0757 [TQ] COG2508 Regulator of polyketide synthase expression Length = 557 Score = 125 bits (314), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 129/545 (23%), Positives = 240/545 (44%), Gaps = 58/545 (10%) Query: 10 ELMQLPILSKATVISGMQGLDRVVRFVDVMEVPDLKGWISEGVMLLTTAYSIRHDPSLLT 69 +++ LP +A V++G G+ ++ V++M+ PD+ +++ +LLTTAYS++++P+ L Sbjct: 10 DVLDLPTFQEAKVVAGENGVSNTIQSVNLMDAPDIVHFLTTDQLLLTTAYSLKNNPNALI 69 Query: 70 ELIYTLDQLGAAALAIKPARFLKEIPQGAIEASNDCGLPIVEIPPEIPYTDITQPVMELL 129 EL+ + + G A L +K RF+ IP + +N G PI+E+P + + + Sbjct: 70 ELVRHMAEQGCAGLGLKTKRFIDTIPDDVLAEANRLGFPIIELPLRYSLGAMLNESLGFI 129 Query: 130 LGRQAMLLRRAEEVYRTLTTMVLENSGIQAVSDNVADFLKAPVALLDTERNIIVSSPSQF 189 L + L A ++R T M+L G + + +A L+APV LL+ ER I+++ + Sbjct: 130 LKERTNELNYALTLHREFTNMILSGKGFSQIIEKLAAILEAPVILLN-ERLDIMAATHKM 188 Query: 190 D--------WSLAE---------------------RP----LSWNIHVDRRTVARLLVDK 216 D W + E P + IH + L+V Sbjct: 189 DKDGFFEVYWFIHEINHAEDTQTYQQCTLPQTNTTNPYRTFFLYPIHSANQQKGFLVVLG 248 Query: 217 EQLDD---MEEVGIEQARLVFALELMRKKVAEDTEFRLRGNFIDELLTPPLPSRHEVERR 273 D ++ + +EQA V + ELM+ E +L+ +F EL+ + E+ R Sbjct: 249 NLSPDAKGLQILALEQAANVISFELMKMLAIEQHARQLKNDFFCELVESLITREEEILNR 308 Query: 274 --ARQLGMNPEHKWEVAVIEGETAPNEETVNRLLEQEARKRG------VTPHAEFRSNRA 325 A QL + ++ + + ++ E +E+E R++ + + + + + Sbjct: 309 GKAYQLDKSLQYVCLASKFDEMSSFQTEVQPLYMEKELRQQREQMFELLNSYLKKHFDHS 368 Query: 326 VLFLPTPEGRKFGV-AGDKGTSWAV--TLEGWLSDKAEGLG-----GYRTGVGT-PEYLW 376 +LF +G F + G + AV T + D + L + GV E + Sbjct: 369 ILF---TKGDLFVILIGFSYYNDAVEKTFIDAIRDIQQELNQMLDFSFSFGVSNYTERVT 425 Query: 377 DIQTSYNEARKALSVAKRLGQGTGGVTRYEEIEVYHLLEGLSGEGFAALFERKLGKLRQY 436 DI T++ EA AL R + + Y E+ L + + + ++ L L Sbjct: 426 DIATAFQEAVDALRSGYRENKKR-FIKTYRIKEMTELFKSIPQQKLKEFYQSSLKDLAFP 484 Query: 437 DQEHDSNMLLTFYHYLECRGSLIETANSLYIHRNSVKYRLERIRDITGFDLNDPREQFVC 496 + + ++L T +L + ETA S++IHRN+V YR+++ +I G DL E Sbjct: 485 ETKEKQDLLETLDSFLNHHCQISETAKSMFIHRNTVIYRIKKCEEILGIDLKGGHETLQL 544 Query: 497 HLCLI 501 + L Sbjct: 545 RMALF 549 >BH1735 [TQ] COG2508 Regulator of polyketide synthase expression Length = 553 Score = 112 bits (281), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 127/545 (23%), Positives = 233/545 (42%), Gaps = 69/545 (12%) Query: 14 LPILSKATVISGMQGLD-RVVRFVDVMEVPDLKGWISEGVMLLTTAYSIRHDPSLLTELI 72 + ++ A V +G L + V +V V+E P ++ ++ E ++LTT D + + Sbjct: 9 VELMGDAVVQTGEASLQTQPVEWVSVIETP-VENFVRENELVLTTGIGCGEDEERFLQFV 67 Query: 73 YTLDQLGAAALAIKPARFLKEIPQGAIEASNDCGLPIVEIPPEIPYTDITQPVMELLLGR 132 + + AAALAI RF+K IP + ++ GL ++EIP E+ + D+ Q V+ + R Sbjct: 68 HDIAASNAAALAIAMGRFVKHIPDRVLHYASTHGLILLEIPWELRFADLIQSVLLQMEQR 127 Query: 133 QAMLLRRAEEVYRTLTTMVLENSGIQAVSDNVADFLKAPVALLDTERNIIVSSPSQFD-- 190 + + +AE + + L M+L + ++++V+ AP+ LLD R +I D Sbjct: 128 KQKEIEQAESLRKQLLEMILNGTTFHQIAEHVSHVFDAPILLLD-RRGVIRGKSENSDQL 186 Query: 191 ---W--SLAERPLSWN------------IHVDRRTVARLLV---------------DKEQ 218 W S + +++ +H D +L++ +E Sbjct: 187 ERIWHSSFSHEVIAYQPSVHAPSGSSKWLHDDESAALQLVIRSAGEIQGYLLIGTASEEH 246 Query: 219 LDDMEEVGIEQARLVFALELMRKKVAEDTEFRLRGNFIDELLTPPLPSRHEVERRARQLG 278 + + E AL + + A++TE+RL+G+F+ L + S RA+ LG Sbjct: 247 TLEQQMALFEHVTTAVALHFLHEHAAKETEWRLQGDFVWSLAKGDVSSWDTALSRAKSLG 306 Query: 279 MN-----------PEHKWEVAVIEGETAPNEET----VNRLLEQEARKRGVTPHAEFRSN 323 PE+ ++ + E + V R LE+EA + H + + Sbjct: 307 YQVHLPYLCLTAMPENISQLYQQDREQIGTFDHWLQGVTRCLEEEAYDVAKSLHLQVMAT 366 Query: 324 RA----VLFLPTPEGRKFGVAGDKGTSWAVTLEGWLSDKAEGLG---GYRTGVGTPEYLW 376 A ++FL V D+ T A T + L + G+ Sbjct: 367 YAKEYLIVFLE--------VVQDRSTETAYTYIQSFQQRMSLLFPHLFFSWGISKTSGYK 418 Query: 377 DIQTSYNEARKALSVAKRLGQGTGGVTRYEEIEVYHLLEGL-SGEGFAALFERKLGKLRQ 435 S+ EARKAL+V ++ +G G + Y + + +L L + + + L L Sbjct: 419 QFHESFEEARKALTVGRK-QRGPGCQSLYADTRIDRVLFSLIDNQELREMTDSLLHSLLV 477 Query: 436 YDQEHDSNMLLTFYHYLECRGSLIETANSLYIHRNSVKYRLERIRDITGFDLNDPREQFV 495 Y + +++ TF Y + ++ +TA L +HR S+ YRL +I +TG LN+P + F+ Sbjct: 478 YSVDRKIDLVNTFQTYHRNKSNVSQTARQLNLHRQSLLYRLRKIESLTGCSLNNPDDVFL 537 Query: 496 CHLCL 500 L + Sbjct: 538 LDLSI 542 >BH1994_2 [TQ] COG2508 Regulator of polyketide synthase expression Length = 313 Score = 93.6 bits (231), Expect = 6e-19, Method: Compositional matrix adjust. Identities = 76/297 (25%), Positives = 139/297 (46%), Gaps = 27/297 (9%) Query: 217 EQLDDMEEVGIEQARLVFALELMRKKVAEDTEFRLRGNFIDELLTPPLPSRHEVERRARQ 276 E++DD ++ I+ A ++A++ ++K+ D +++ +F+++LL + + + + A Sbjct: 10 EEIDDFYQLSIDLALNIYAIQFNKQKLVLDAREQVKDSFLNKLLVEKIEDQKSIIQYANL 69 Query: 277 LGMNPEHKWEVAVIEGETAPNEETVNRLLEQEARKRGVTPHAEFR-------------SN 323 + H V+V+ A E LLEQ+A K + + + R + Sbjct: 70 FHWDLFHSHRVSVLSINLAQKESNKVNLLEQQAMKSLLWENIKTRLSIYDEDILLSSKGD 129 Query: 324 RAVLFLPTPEGRKFGVAGDKGTSWAV---TLEGWLSDKAEGLGG-YRTGVG-TPEYLWDI 378 +L +P + R G WA ++ W+ + EG+ G+G E + D Sbjct: 130 EYILIVPASKER-----GKPKVYWAKVYQNIQSWV--EQEGIRARLLLGIGGKTETIHDY 182 Query: 379 QTSYNEARKALSVAKRLGQGTGGVTRYEEIEVYHLLEGLSGEGFAALF-ERKLGKLRQYD 437 + Y +A + +V + G ++E+ Y LL L +LF ++ L L QY Sbjct: 183 FSCYQQAVQVHNVVSHRFRDIG-FALFDELGSYTLLHHLKNTPATSLFIDKHLQPLLQYS 241 Query: 438 QEHDSNMLLTFYHYLECRGSLIETANSLYIHRNSVKYRLERIRDITGFDLNDPREQF 494 Q +++ T YL GS+ ET++ LYIHR+S+ YRLE+I + DLND +F Sbjct: 242 QGRSTDLFYTLRVYLNQNGSIKETSDELYIHRSSLLYRLEKIETLLDVDLNDSEHRF 298 >CAC1426 [TQ] COG2508 Regulator of polyketide synthase expression Length = 397 Score = 86.7 bits (213), Expect = 8e-17, Method: Compositional matrix adjust. Identities = 36/128 (28%), Positives = 73/128 (57%) Query: 7 TLRELMQLPILSKATVISGMQGLDRVVRFVDVMEVPDLKGWISEGVMLLTTAYSIRHDPS 66 T + +++LP L K V++G +GL+R++ +V V+E P+ W+ G ++L + +I+++ + Sbjct: 4 TCKSVLKLPYLEKIKVVAGEKGLNRIITWVQVVEYPEYSKWLKGGELILISGIAIKNNIN 63 Query: 67 LLTELIYTLDQLGAAALAIKPARFLKEIPQGAIEASNDCGLPIVEIPPEIPYTDITQPVM 126 L I ++ + + I ++KEIP+ IE N PI EIP E+ + D++Q + Sbjct: 64 SLVNFIRDINSRSLSGIVISIGPYIKEIPKEVIEIGNQLDFPIFEIPFEVKFIDVSQSIC 123 Query: 127 ELLLGRQA 134 + + + Sbjct: 124 KAIFANKV 131 Score = 71.2 bits (173), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 67/254 (26%), Positives = 110/254 (43%), Gaps = 25/254 (9%) Query: 254 NFIDELLTPPLPSRHEVERRARQLGMNPEHKWEVAVIEGETAPN----EETVNRLLEQ-- 307 NF+ +++ ++ RA G NP+ K+ V+E + + + +EQ Sbjct: 138 NFMKDIIFRDFTFSEDILSRAVAYGYNPKTKYISFVVELHNEKDMLECDLKTKQYIEQIV 197 Query: 308 -----EARKRGVTPHAEFRSNRAVLFLPTPEGRKFGVAGDKGTSWAVTLEGWLSDKAEGL 362 E KR + H + N +L LP E R + A+ + + KA+ Sbjct: 198 IEVLGEWNKRSI--HI-MQYNSIILMLPKNEKRTIK-------NIAMNIIEEIISKAKN- 246 Query: 363 GGYRTGVGTP-EYLWDIQTSYNEARKALSVAKRLGQGTGGVTRYEEIEVYHLLEGLSG-E 420 GVG L D + S +EA + L V K L V Y+E+ +Y L + E Sbjct: 247 TSVNIGVGGEFSELRDFKISVSEAHRCLKVLK-LNNDNNTVISYDELGIYKLFFQMDNLE 305 Query: 421 GFAALFERKLGKLRQYDQEHDSNMLLTFYHYLECRGSLIETANSLYIHRNSVKYRLERIR 480 + L KL YD ++ + ++ T Y++ ++ TA L+IHRN++KYR+ RI Sbjct: 306 EMKKFYLDVLKKLIDYDSKNTTELMNTLEMYIKESTNINRTAEKLFIHRNTLKYRIRRIE 365 Query: 481 DITGFDLNDPREQF 494 +IT L D F Sbjct: 366 EITKCSLKDVNVLF 379 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.137 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 38,680,667 Number of extensions: 1654948 Number of successful extensions: 6702 Number of sequences better than 1.0e-05: 14 Number of HSP's gapped: 6698 Number of HSP's successfully gapped: 18 Length of query: 508 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 397 Effective length of database: 25,576,357 Effective search space: 10153813729 Effective search space used: 10153813729 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 118 (50.1 bits)