BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0386.0034.p
         (297 letters)

Database: COG 
           115,334 sequences; 38,378,431 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NMA1127 [J] COG0566 rRNA methylases                                    58   2e-08
Cj0261c [QR] COG0500 SAM-dependent methyltransferases                  57   5e-08
FN0197 [QR] COG0500 SAM-dependent methyltransferases                   54   2e-07

>NMA1127 [J] COG0566 rRNA methylases
          Length = 275

 Score = 58.2 bits (139), Expect = 2e-08,   Method: Compositional matrix adjust.
 Identities = 41/133 (30%), Positives = 68/133 (51%), Gaps = 14/133 (10%)

Query: 73  NASVLDIGAASGVFSVPFAEKGARVTAVETSPPLIELLEENVANFAEGQVKIVPEPFEDI 132
           ++ VLDIGA  G  ++P A+    VTAVE S  ++  L+EN+       V  + + +EDI
Sbjct: 62  DSKVLDIGAGPGTLTIPLADMVKHVTAVEPSDGMLYCLKENIKAKELNNVSWIQKKWEDI 121

Query: 133 DVASMGWNQTFDLVFASM---IPIIHNWESVEKILQCARKFCYIS----MPVLHTEHSLV 185
           D+  +  +  +D+V AS    +P I   E+V K+   + K+ Y++    MP     ++  
Sbjct: 122 DLKDL--DAPYDVVVASFSLSMPDIK--EAVIKMNNVSSKYVYLNWFAGMPSWEKTYA-- 175

Query: 186 KEIWPLVTGQSYQ 198
            E W  + G SY 
Sbjct: 176 -EAWKRIHGTSYN 187


>Cj0261c [QR] COG0500 SAM-dependent methyltransferases
          Length = 239

 Score = 56.6 bits (135), Expect = 5e-08,   Method: Compositional matrix adjust.
 Identities = 30/121 (24%), Positives = 59/121 (48%), Gaps = 12/121 (9%)

Query: 58  KRIISWLEGQGVQFENASVLDIGAASGVFSVPFAEKGARVTAVETSPPLIELLEENVANF 117
           K+   +L+   + F++ S++DIG  +GV+++  A++   + A++++  ++E+L+E+    
Sbjct: 23  KQTFEYLQNLKISFQDKSIIDIGCGTGVWTLHLAKEAKEILALDSANAMLEILQEDAKKL 82

Query: 118 AEGQVKIVPEPFEDIDVASMGWNQT-----FDLVFASMIPIIHNWESVEKILQCARKFCY 172
               +K     FE        W Q      FDL F SM P + N +     L  A+   Y
Sbjct: 83  NLNNIKCKNLSFE-------TWMQNNPNVKFDLAFLSMSPALQNEKDYTNFLNLAKIKIY 135

Query: 173 I 173
           +
Sbjct: 136 L 136


>FN0197 [QR] COG0500 SAM-dependent methyltransferases
          Length = 266

 Score = 54.3 bits (129), Expect = 2e-07,   Method: Compositional matrix adjust.
 Identities = 38/156 (24%), Positives = 79/156 (50%), Gaps = 8/156 (5%)

Query: 32  DPTRSFDHKAKVFNEQSFNEKGRQRTKRIISWLEGQGVQFENASVLDIGAASGVFSVPFA 91
           D  + +D K++ F +++  ++     + I   ++ + +  EN+ VLDIG  +G   + F+
Sbjct: 21  DLEKIWDSKSEWFFKRTEKKQENFSDRLIFRMIKEKNLLNENSKVLDIGCGTGRHLLEFS 80

Query: 92  EKGARVTAVETSPPLIELLEENVANFAEGQVKIVPEPFEDIDVASMGWNQTFDLVFASMI 151
           +  + VT  + S  +++  +E + N  E   K V   +    + +    + FDLVFASM 
Sbjct: 81  KFTSYVTGTDISSKMLDYAKEKLKNVREA--KFVHGNW----MENFTKEKEFDLVFASMT 134

Query: 152 PIIHNWESVEKILQCARKFCYISMPVLHTEHSLVKE 187
           P I   E ++++   ++K+C +   V +    L++E
Sbjct: 135 PAITTIEHIKRMCLISKKYCLMERFVYN--RDLIRE 168


  Database: COG
    Posted date:  Aug 3, 2010  9:58 AM
  Number of letters in database: 38,378,431
  Number of sequences in database:  115,334
  
Lambda     K      H
   0.318    0.133    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 115334
Number of Hits to DB: 21,299,985
Number of extensions: 822214
Number of successful extensions: 1682
Number of sequences better than 1.0e-05: 3
Number of HSP's gapped: 1689
Number of HSP's successfully gapped: 3
Length of query: 297
Length of database: 38,378,431
Length adjustment: 106
Effective length of query: 191
Effective length of database: 26,153,027
Effective search space: 4995228157
Effective search space used: 4995228157
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 115 (48.9 bits)