BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0386.0033.p (477 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin0519 [GEPR] COG0477 Permeases of the major facilitator superf... 474 e-133 BS_lmrB [GEPR] COG0477 Permeases of the major facilitator superf... 474 e-133 lin1658 [GEPR] COG0477 Permeases of the major facilitator superf... 344 2e-94 L132251 [GEPR] COG0477 Permeases of the major facilitator superf... 228 1e-59 BH0554 [GEPR] COG0477 Permeases of the major facilitator superfa... 181 2e-45 >lin0519 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 471 Score = 474 bits (1220), Expect = e-133, Method: Compositional matrix adjust. Identities = 236/464 (50%), Positives = 327/464 (70%), Gaps = 1/464 (0%) Query: 5 NNIRRGPIMLALIIGMFVAALNETLMGNALPALMQSFDASAATVQWLSTAYMLVVGVLVP 64 IR PI+ + ++ F+ +ET + AL L+Q FD S+ATVQWL+T Y+L +G+LVP Sbjct: 9 QKIRPIPIIASFLMAGFIGLFSETALNMALSDLIQVFDISSATVQWLTTGYLLTLGILVP 68 Query: 65 ITAILQQWFTTRQLFLSAMGLFFIGTVISAVSPVFSVLLIGRIIQAVGTGMLLPLVMNVI 124 I+ +L QWFTTR LF +A+ GT+I+A+SP F++L+IGR++QAVGT +LLPL+ N I Sbjct: 69 ISGLLLQWFTTRGLFFTAVSFSIAGTLIAALSPTFAMLMIGRVVQAVGTALLLPLMFNTI 128 Query: 125 MTIYPPEKRXXXXXXXXXXXXFAPAIGPTISGLIVDGLSWRWLFYLVIPFALISIFVGAA 184 + I+P KR FAPA+GPTISGLI++ L+W W+F++ +PF +I++ G Sbjct: 129 LLIFPEHKRGSAMGMIGLVIMFAPAVGPTISGLILENLTWNWIFWISLPFLIIALLFGMK 188 Query: 185 VLKNVSEVTKPRIDFLSIVLSTIGFGGIVYGFSSAGEHGWAVPEVIWTIAVGGVALLLFV 244 ++NVS VTKP+ID LSI+LST+GFGG+V+ FSSAGE+GW V+ +I VGG+AL LFV Sbjct: 189 FMQNVSVVTKPKIDILSIILSTLGFGGVVFAFSSAGENGWGSATVLVSIIVGGIALGLFV 248 Query: 245 WRQLKLEQPIMDLRAFRYPXXXXXXXXXXXXXXXXXSSAIMLPMFMQGVLLITAFKSGLI 304 WRQL +E+P+MDL+ F+YP S+ I+LP+++Q L + AF +GL+ Sbjct: 249 WRQLTMEKPLMDLKVFKYPMFTLGLILVFISFMMILSTMILLPLYLQNSLALAAFSAGLV 308 Query: 305 LLPGGIVNGVMAPISGKLFDKFGPRILIIPGLTIAVISLWQFTRFNEATSTGFIVSVHIA 364 LLPGG++NG+M+P +G+LFD +GPR L+IPG +AVI+L+ TR TS I+ +H Sbjct: 309 LLPGGVLNGLMSPFTGRLFDAYGPRALVIPGFIVAVIALFFLTRIEVGTSALTIIVLHSV 368 Query: 365 LMIGIALVMMPAQTTGLNQLPRNLHPHGTAILNTLQQVAGAIGTALYISIMSGGQKQYLS 424 LMIGI++VMMPAQT GLNQLP L+P GTAI+NTLQQV+GAIGTA+ I+IMS GQK Y+ Sbjct: 369 LMIGISMVMMPAQTNGLNQLPPKLYPDGTAIMNTLQQVSGAIGTAVAITIMSAGQKAYME 428 Query: 425 GVSDPSASSEITKGLAAGINNAFWFGVIVALIALGIGLFLRKGK 468 + ++ L AGI NAF FG+I+A I L LF+RK K Sbjct: 429 -TAQGVGPEQMIASLTAGIQNAFVFGLIMACIGLLCSLFIRKAK 471 >BS_lmrB [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 479 Score = 474 bits (1219), Expect = e-133, Method: Compositional matrix adjust. Identities = 235/464 (50%), Positives = 331/464 (71%), Gaps = 3/464 (0%) Query: 11 PIMLALIIGMFVAALNETLMGNALPALMQSFDASAATVQWLSTAYMLVVGVLVPITAILQ 70 PIM++L++ F+ +ET + AL LM+ + +AATVQWL+T Y+LV+G+LVP++ +L Sbjct: 17 PIMISLLLAGFIGMFSETALNIALTDLMKELNITAATVQWLTTGYLLVLGILVPVSGLLL 76 Query: 71 QWFTTRQLFLSAMGLFFIGTVISAVSPVFSVLLIGRIIQAVGTGMLLPLVMNVIMTIYPP 130 QWFTTRQLF ++ +GT I+A++P FS LL RI+QA+GTG+LLPL+ N I+ I+PP Sbjct: 77 QWFTTRQLFTVSLIFSILGTFIAALAPSFSFLLAARIVQALGTGLLLPLMFNTILVIFPP 136 Query: 131 EKRXXXXXXXXXXXXFAPAIGPTISGLIVDGLSWRWLFYLVIPFALISIFVGAAVLKNVS 190 KR FAPAIGPT SGL+++ L+W W+F++ +PF ++++ G A ++NVS Sbjct: 137 HKRGAAMGTIGLVIMFAPAIGPTFSGLVLEHLNWHWIFWISLPFLVLALVFGIAYMQNVS 196 Query: 191 EVTKPRIDFLSIVLSTIGFGGIVYGFSSAGE--HGWAVPEVIWTIAVGGVALLLFVWRQL 248 E TKP+ID LSI+LSTIGFGGIV+GFS+AGE GW+ P VI ++ VG V L+LF RQL Sbjct: 197 ETTKPKIDVLSIILSTIGFGGIVFGFSNAGEGSGGWSSPTVIVSLIVGVVGLILFSIRQL 256 Query: 249 KLEQPIMDLRAFRYPXXXXXXXXXXXXXXXXXSSAIMLPMFMQGVLLITAFKSGLILLPG 308 ++QP+M+LRAF+YP SS ++LPM++QG L++TAF SGL+LLPG Sbjct: 257 TMKQPMMNLRAFKYPMFILGVIMVFICMMVILSSMLLLPMYLQGGLVLTAFASGLVLLPG 316 Query: 309 GIVNGVMAPISGKLFDKFGPRILIIPGLTIAVISLWQFTRFNEATSTGFIVSVHIALMIG 368 GI+NG M+P++G+LFDK+GP+ L+IPG I + LW F+ ++ I+ +H LMIG Sbjct: 317 GILNGFMSPVTGRLFDKYGPKWLVIPGFVIVTVVLWFFSNVTTTSTAVLIIILHTCLMIG 376 Query: 369 IALVMMPAQTTGLNQLPRNLHPHGTAILNTLQQVAGAIGTALYISIMSGGQKQYLSGVSD 428 I+++MMPAQT GLNQLPR +P GTAI+NTLQQ+AGAIGTA+ +SIM+ GQ Y+S V + Sbjct: 377 ISMIMMPAQTNGLNQLPREFYPDGTAIMNTLQQMAGAIGTAVAVSIMAAGQHDYMSTVKN 436 Query: 429 PSASSEITKGLAAGINNAFWFGVIVALIALGIGLFLRKGKSPEH 472 P+ + I + L AG+ +AF F +IVA+I L IG F K +H Sbjct: 437 PADPAVIPQALTAGVQHAFVFAMIVAIIGL-IGAFFMKRVKVDH 479 >lin1658 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 489 Score = 344 bits (882), Expect = 2e-94, Method: Compositional matrix adjust. Identities = 184/470 (39%), Positives = 292/470 (62%), Gaps = 5/470 (1%) Query: 5 NNIRRGPIMLALIIGMFVAALNETLMGNALPALMQSFDASAATVQWLSTAYMLVVGVLVP 64 +++R I++ +++G FV LN+TLM ALP++M+ F +A+ QWLST +MLV GV++P Sbjct: 6 TSVKRNGILIVMLMGAFVTILNQTLMNVALPSIMKDFGITASQGQWLSTGFMLVNGVMIP 65 Query: 65 ITAILQQWFTTRQLFLSAMGLFFIGTVISAVSPVFSVLLIGRIIQAVGTGMLLPLVMNVI 124 +TA L + FTTRQL+L AM F IGT I + +++L+ GR++QA+G G+++PL+ V+ Sbjct: 66 MTAFLIERFTTRQLYLFAMITFAIGTAIGGFATDYTMLIAGRMVQAIGAGIVMPLLTVVV 125 Query: 125 MTIYPPEKRXXXXXXXXXXXXFAPAIGPTISGLIVDGLSWRWLFYLVIPFALISIFVGAA 184 + ++P E+R FAPAIGPT+SG IV WR LF+++IPFA++ I V Sbjct: 126 LNLFPMERRGRAMGLIGLAMNFAPAIGPTLSGWIVQQYDWRNLFFIIIPFAILDIVVAIF 185 Query: 185 VLKNVSEVTKPRIDFLSIVLSTIGFGGIVYGFSSAGEHGWAVPEVIWTIAVGGVALLLFV 244 +LKNV + T P++D L +++ST+GFG ++ GFS+AG+H W +V I +G V L +F+ Sbjct: 186 LLKNVGKRTFPKLDILGVIMSTVGFGSLLLGFSNAGDHDWLTWKVAGFIILGLVVLGIFI 245 Query: 245 WRQLKLEQPIMDLRAFRYPXXXXXXXXXXXXXXXXXSSAIMLPMFMQGVLLITAFKSGLI 304 Q + P+++ + F+YP ++LP+F+Q V + +SGL+ Sbjct: 246 RYQSSNKAPLLNFKVFKYPTFALTTAISFFVVMGLFGGMLLLPIFLQTVRGFSPLESGLV 305 Query: 305 LLPGGIVNGVMAPISGKLFDKFGPRILIIPGLTIAVISLWQFTRFNEATSTGFIVSVHIA 364 LLPG +V +++P++G +FD+FG + L + GL I S + FT +E+T+ FI+ V Sbjct: 306 LLPGALVTAILSPVTGVMFDRFGAKYLSLVGLIIMAGSTFMFTNLDESTTLTFIIIVQTI 365 Query: 365 LMIGIALVMMPAQTTGLNQLPRNLHPHGTAILNTLQQVAGAIGTALYISIMSGGQKQYLS 424 G+A+VMMP QT LN LP L HG+A+ NT++QVAG+IGTA I++MS + Sbjct: 366 RSAGMAMVMMPLQTAALNSLPLKLASHGSAMFNTMRQVAGSIGTAALITVMSKSAASFAD 425 Query: 425 --GVSD--PSASSEITKG-LAAGINNAFWFGVIVALIALGIGLFLRKGKS 469 G +D +E+ L GI AF I++++A + LF++K +S Sbjct: 426 KLGPADVIGKTKTEVANHVLIHGIETAFLVAGILSVVACILALFIQKNRS 475 >L132251 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 514 Score = 228 bits (582), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 152/499 (30%), Positives = 258/499 (51%), Gaps = 36/499 (7%) Query: 5 NNIRRGPIMLALIIGMFVAALNETLMGNALPALMQSFDASAATVQWLSTAYMLVVGVLVP 64 + R +++ +++G F A L +T +G A+P LM F+ S +T Q +T ++L G++VP Sbjct: 16 KHYSRIGMLILILVGTFGAMLMQTSLGTAIPTLMNDFNISMSTAQQATTWFLLANGIMVP 75 Query: 65 ITAILQQWFTTRQLFLSAMGLFFIGTVISAVSPVFS--VLLIGRIIQAVGTGMLLPLVMN 122 I+A L F TR L+++A FIG ++ +P S V L GRI+QA+ G+ +PL+ Sbjct: 76 ISAYLATKFPTRWLYVTAYATLFIGMFVAYSAPTSSWWVFLAGRILQAIAVGITMPLMQV 135 Query: 123 VIMTIYPPEKRXXXXXXXXXXXXFAPAIGPTISGLIVD----------GLSWRWLFYLVI 172 ++ ++P ++ APA+GPT +G ++ SWR +F +I Sbjct: 136 ALVNMFPAKQMGAIMGVAGVVLVLAPALGPTFAGWLITHKFNLIFWTFAASWRTIF--LI 193 Query: 173 PFA------LISIFVGAAVLKNVSEVTKP-RIDFLSIVLSTIGFGGIVYGFSSAGEHGWA 225 P LISIF V+ N +P ++DFLS++LS +GFG ++GF++ GW Sbjct: 194 PMIVVGVVFLISIFAMRDVIPN-----RPMKLDFLSVLLSVVGFGAFLWGFTNVATDGWG 248 Query: 226 VPEVIWTIAVGG-VALLLFVWRQLKLEQPIMDLRAFRYPXXXXXXXXXXXXXXXXXSSAI 284 + VGG + + LF RQLK++ P +D+R F+ + Sbjct: 249 KFNTVIAPIVGGLIIIFLFGLRQLKMDDPFLDIRVFKIRQFTLVTLALALSTMAMMGVEM 308 Query: 285 MLPMFMQGVLLITAFKSGLILLPGGIVNGVMAPISGKLFDKFGPRILIIPGLTIAVISLW 344 MLP+++Q V ++AF SGL+LLPG ++ V+AP++G ++D+ G + L + G + + Sbjct: 309 MLPLYLQDVHGLSAFNSGLVLLPGSLMMAVVAPLAGGVYDRIGSKRLAVIGFLVLALGTI 368 Query: 345 QFTRFNEATSTGFIVSVHIALMIGIALVMMPAQTTGLNQLPRNLHPHGTAILNTLQQVAG 404 F F T F ++ M+G+ +VMMP T+ +N +P N TA NT +QVA Sbjct: 369 PFMFFTATTPDHFDTLLYGLRMVGVGMVMMPLTTSAMNAVPVNEVAQATASNNTARQVAS 428 Query: 405 AIGTALYISIMSGGQKQYLSGVSDPSAS-----SEITKGLAAGINNAFWFGVIVALIALG 459 ++ AL S+ + S S + ++ + + G + +F G++ A+I +G Sbjct: 429 SVVVALLSSVAQNVITNNMPASSLKSTNPLQFANKAIEAMLQGYHASFAIGLVFAIIGIG 488 Query: 460 IGLFLRKGKS----PEHEQ 474 + LFL+ G+ P EQ Sbjct: 489 VALFLKSGRMISGVPVEEQ 507 >BH0554 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 474 Score = 181 bits (459), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 129/478 (26%), Positives = 234/478 (48%), Gaps = 15/478 (3%) Query: 4 LNNI-RRGPIMLALIIGMFVAALNETLMGNALPALMQSFDASAATVQWLSTAYMLVVGVL 62 + NI ++ ++ A++ G F LN +++ A+P LM F+A A W+ T +M+ +G+ Sbjct: 1 MKNIPKKWMVVCAVLFGSFTMILNNSMLNPAIPQLMNVFEADAVATGWVITIFMVAMGMT 60 Query: 63 VPITAILQQWFTTRQLFLSAMGLFFIGTVISAVSPVFSVLLIGRIIQAVGTGMLLPLVMN 122 +P+T L ++ ++ +G+F +G+++ A+S L++ R +Q +G G+++PL M Sbjct: 61 MPLTGYLGDKIGKKEAYILGLGIFVLGSLLGALSWNLPSLIVFRGLQGIGGGVMMPLSMT 120 Query: 123 VIMTIYPPEKRXXXXXXXXXXXXFAPAIGPTISGLIVDGLSWRWLFYLVIPFALISIFVG 182 +I +P +R AP IGPT+ G IV+ +W++LF + IPF L+ I Sbjct: 121 LIFDAFPRNERGLATGVWGVASMMAPTIGPTLGGFIVETSNWKYLFLVNIPFGLLGIIAA 180 Query: 183 AAVLKNVSEVTKPRIDFLSIVLSTIGFGGIVYGFSSAGE--HGWAVPEVIWTIAVGGVAL 240 L ++ + ++D + T G G I+ F + H + E I + + AL Sbjct: 181 VIYLPKIARSGQIKLDRWGFLFVTAGVGSILLAFGRMSDLTH---LTEWINGVLLLLGAL 237 Query: 241 LLFVWRQL--KLEQPIMDLRAFRYPXXXXXXXXXXXXXXXXXSSAIMLPMFMQGVLLITA 298 L+V+ Q+ + EQP++DL FR P + ++P+ +Q V Sbjct: 238 CLYVFVQVEKRAEQPLLDLSLFRIPAYSLSIWVAGISSIGLFAGIFLVPLLLQQVYDYGP 297 Query: 299 FKSGLILLPGGIVNGVMAPISGKLFDKFGPRILIIPGLTIAVISLWQFTRFNEATSTGFI 358 +GL+ LP + G+ I G++ DK GP ++ G+ IA + + + T +I Sbjct: 298 IMTGLVFLPSALFTGLTMSIGGRMLDKRGPSGIMTAGMMIAAVGTFALGYLHLETGLWYI 357 Query: 359 VSVHIALMIGIALVMMPAQTTGLNQLPRNLHPHGTAILNTLQQVAGAIGTALYISIMSGG 418 + +G+ L MPA TTG+N +P L G+A+ N L+Q++ A G ++IS+ Sbjct: 358 FAWMAIRGVGMGLTTMPATTTGMNAIPEGLISRGSAMNNVLRQMSSAFGI-VFISVFFEV 416 Query: 419 QKQYLSGVSDPSASSEITKGLAAGINNAFWF-GVIVALIALGIGLFLRKGKSPEHEQL 475 ++ L+ V S+ + IN F+ G + AL +K K E E++ Sbjct: 417 RRGQLALV-----STSFEEATLQAINEGFFIVGFLTALSIPAAYWLEKKAKQGEQERV 469 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.327 0.142 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 31,800,421 Number of extensions: 1232942 Number of successful extensions: 11989 Number of sequences better than 1.0e-05: 78 Number of HSP's gapped: 11866 Number of HSP's successfully gapped: 78 Length of query: 477 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 367 Effective length of database: 25,691,691 Effective search space: 9428850597 Effective search space used: 9428850597 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 118 (50.1 bits)