BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0386.0031.p (459 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC0883 [V] COG0534 Na+-driven multidrug efflux pump 418 e-117 FN1726 [V] COG0534 Na+-driven multidrug efflux pump 347 3e-95 lin2873 [V] COG0534 Na+-driven multidrug efflux pump 312 7e-85 MA2050 [V] COG0534 Na+-driven multidrug efflux pump 305 9e-83 NMA2050 [C] COG0282 Acetate kinase 305 9e-83 >CAC0883 [V] COG0534 Na+-driven multidrug efflux pump Length = 448 Score = 418 bits (1075), Expect = e-117, Method: Compositional matrix adjust. Identities = 212/433 (48%), Positives = 314/433 (72%), Gaps = 8/433 (1%) Query: 4 LNE--QLGSEPIRKLLLKFSIPAIAGMLVSSLYNLLDRIFVGR-LGALAMTGIGLTLPFM 60 +NE +L +E + KLLLKFSIP+I GM+V++LY+++DRIFVGR + + A++G+ +T P Sbjct: 1 MNEPNELETEGVWKLLLKFSIPSIIGMVVNALYSIVDRIFVGRGVNSTALSGVAITYPIS 60 Query: 61 TLLTAFTSLVGIGTSAIVSMRLGQNRKEDAEKVLGNAFSMLTVLMLVVTAIGLIFKEPIL 120 ++ AF L GIG SA+VS++LGQ + + AEK++GN F++L + LVVT GLIF EP+L Sbjct: 61 NVILAFGMLAGIGCSALVSIKLGQKKVKQAEKIIGNTFTLLIIFSLVVTFFGLIFLEPML 120 Query: 121 TLLGASELTMGYASDYITIILIGSVFQGIGAGLSNVIRAEGHPAKATIVLVIGTVLDMIL 180 LLGAS+ TM YA + IIL G + Q +G GL++ +RAEG P A ++IG VL+ I+ Sbjct: 121 KLLGASKNTMPYAKQFAVIILSGVILQNMGFGLNSTMRAEGDPKMAMKTMLIGAVLNFIM 180 Query: 181 NPLFIFTFNMGIAGAAWTTVLSATVTTILVIAHFVGKKSVLKLRIVNLKLSLPIVKDILS 240 NPLFIF +G+ G+A T++S V ++ V+ +F+ KS LK++ N++L +V++++S Sbjct: 181 NPLFIFVLKLGVRGSALATIVSQAVCSVWVLYYFIKGKSFLKIKRENIRLRREVVREVIS 240 Query: 241 IGLSPFFMQISAFIVGIISNNALKTYGGDVAIGAMTVVNAVMILFLMSAMGITQGAQPIL 300 IG+SPF MQ++A ++ I N +L+ YGGD +I A ++++++ +L LM GI QG+QPI+ Sbjct: 241 IGMSPFAMQLAASLITITFNKSLEKYGGDTSIAAFSLISSIQMLILMPIFGINQGSQPII 300 Query: 301 GFNYGAQQYDRVKKTLKLELALVTAICV--LTFLMVQIFPTGLARILS-NDLELINAASH 357 G+NYGA+ RVKK L A+++A+C+ L F++VQIFPT + + + ND++LI + Sbjct: 301 GYNYGAKNIKRVKKA--LWYAVLSALCISFLGFIIVQIFPTQIISLFNKNDMKLIGVGTR 358 Query: 358 GMKIFLLMLPIIGAQIVGASYFQAVGAAKKAMFLGLLRQVITLIPMLIILPNFFGLTGVW 417 G+ IFLLMLP+IG Q V +YFQAVG AKK+M L LLRQVI L+P LI+LP FFGL G+W Sbjct: 359 GISIFLLMLPVIGPQAVCTNYFQAVGKAKKSMLLSLLRQVIFLLPSLIVLPRFFGLNGIW 418 Query: 418 AAAPTSDFISCSV 430 AA PTSDF++ +V Sbjct: 419 AAGPTSDFLAFAV 431 >FN1726 [V] COG0534 Na+-driven multidrug efflux pump Length = 457 Score = 347 bits (889), Expect = 3e-95, Method: Compositional matrix adjust. Identities = 191/462 (41%), Positives = 290/462 (62%), Gaps = 9/462 (1%) Query: 1 MSTLNEQLGSEPIRKLLLKFSIPAIAGMLVSSLYNLLDRIFVGRL---GALAMTGIGLTL 57 M + + +E I KLL+KFSIPAI GM V++LYN++DRI++G + G L +TGIG+ Sbjct: 1 MENKHNFMETESITKLLIKFSIPAIVGMFVNALYNVVDRIYIGNIKDIGHLGITGIGVVF 60 Query: 58 PFMTLLTAFTSLVGIGTSAIVSMRLGQNRKEDAEKVLGNAFSMLTVLMLVVTAIGLIFK- 116 P + L+ AF+ L+GIG++A VS++LG +E+AE+ LG A L+ ++ VV I + F Sbjct: 61 PVVILIFAFSLLIGIGSAAAVSLKLGMKDREEAERFLGVA-VFLSFIVSVVLMIIIYFNM 119 Query: 117 EPILTLLGASELTMGYASDYITIILIGSVFQGIGAGLSNVIRAEGHPAKATIVLVIGTVL 176 + I+ +G S T YA DY+ I +G +G L++VIR++G P A L++G + Sbjct: 120 DKIIYFIGGSNETFIYAKDYLFYINLGVPAAILGLVLNSVIRSDGSPKIAMGTLLVGAIT 179 Query: 177 DMILNPLFIFTFNMGIAGAAWTTVLSATVTTILVIAHFVGKKSVLKLRIVNLKLSLPIVK 236 +++L+P+FIF F MG+ GAA T++S V+ + I +F KS +KL ++K + K Sbjct: 180 NIVLDPIFIFGFGMGVKGAAIATIISQYVSMLWTIYYFTSNKSKIKLVKKDIKFNFYKAK 239 Query: 237 DILSIGLSPFFMQISAFIVGIISNNALKTYGGDVAIGAMTVVNAVMILFLMSAMGITQGA 296 +I +G S F +Q+ +V I N LK YGGD +IGAM +V + M M GI QG Sbjct: 240 EICLLGSSAFAIQLGFSLVTYILNTVLKKYGGDTSIGAMAIVQSFMTFMAMPIFGINQGI 299 Query: 297 QPILGFNYGAQQYDRVKKTLKLELALVTAICVLTFLMVQIFPTGLARILSNDLELINAAS 356 QPILG+NYGA++Y RVK+ L + T IC++ + V++F L +I + EL Sbjct: 300 QPILGYNYGAEKYKRVKEALYKGIFAATIICIIGYTSVRLFSDTLIKIFTTKPELQEITK 359 Query: 357 HGMKIFLLMLPIIGAQIVGASYFQAVGAAKKAMFLGLLRQVITLIPMLIILPNFFGLTGV 416 +G+K + ++ PI+G QIV + YFQAVG + + F+ L RQ+I +IP LIILP FFGL G+ Sbjct: 360 YGLKAYTMVFPIVGFQIVSSIYFQAVGKPRMSFFISLSRQIIVMIPCLIILPIFFGLNGI 419 Query: 417 WAAAPTSDFISCSVAIVFVAREIKRLGKAEQHQAEVIEHRSL 458 W AAPT+D I+ + + V +EIK+L K E E++E R++ Sbjct: 420 WYAAPTADSIATLITYILVRKEIKKLDKLE----EMLELRNI 457 >lin2873 [V] COG0534 Na+-driven multidrug efflux pump Length = 1091 Score = 312 bits (799), Expect = 7e-85, Method: Compositional matrix adjust. Identities = 33/448 (7%), Positives = 90/448 (20%), Gaps = 1/448 (0%) Query: 1 MSTLNEQLGSEPIRKLLLKFSIPAIAGMLVSSLYNLLDRIFVGR-LGALAMTGIGLTLPF 59 M ++ + + + + Sbjct: 1 MHRYVQKDIQGTTEPQFRNSHSRTSGTVTLYLPVSTFVGTGFFIHPQSKEKLMKRHLNTC 60 Query: 60 MTLLTAFTSLVGIGTSAIVSMRLGQNRKEDAEKVLGNAFSMLTVLMLVVTAIGLIFKEPI 119 L+ + + S + R + A + + +VV + + Sbjct: 61 YRLVWNHMTGAFVVASELARARGKRGGVAVALSLAAVTSLPVLAADIVVHPGETVNGGTL 120 Query: 120 LTLLGASELTMGYASDYITIILIGSVFQGIGAGLSNVIRAEGHPAKATIVLVIGTVLDMI 179 T + G + G + T + Sbjct: 121 ANHDNQIVFGTTNGMTISTGLEYGPDNEANTGGQWVQDGGTANKTTVTSGGLQRVNPGGS 180 Query: 180 LNPLFIFTFNMGIAGAAWTTVLSATVTTILVIAHFVGKKSVLKLRIVNLKLSLPIVKDIL 239 ++ I + + +K ++ Sbjct: 181 VSDTVISAGGGQSLQGRAVNTTLNGGEQWMHEGAIATGTVINDKGWQVVKPGTVATDTVV 240 Query: 240 SIGLSPFFMQISAFIVGIISNNALKTYGGDVAIGAMTVVNAVMILFLMSAMGITQGAQPI 299 + G + + +A++T + + + T + Sbjct: 241 NTGAEGGPDAENGDTGQFVRGDAVRTTINKNGRQIVRAEGTANTTVVYAGGDQTVHGHAL 300 Query: 300 LGFNYGAQQYDRVKKTLKLELALVTAICVLTFLMVQIFPTGLARILSNDLELINAASHGM 359 G QY T + ++ V T + A + + Sbjct: 301 DTTLNGGYQYVHNGGTASDTVVNSDGWQIVKNGGVAGNTTVNQKGRLQVDAGGTATNVTL 360 Query: 360 KIFLLMLPIIGAQIVGASYFQAVGAAKKAMFLGLLRQVITLIPMLIILPNFFGLTGVWAA 419 K ++ A + G + A + +L L + + Sbjct: 361 KQGGALVTSTAATVTGINRLGAFSVVEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTL 420 Query: 420 APTSDFISCSVAIVFVAREIKRLGKAEQ 447 + + +V++ + G A Sbjct: 421 DVRNGGTATTVSMGNGGVLLADSGAAVS 448 >MA2050 [V] COG0534 Na+-driven multidrug efflux pump Length = 468 Score = 305 bits (781), Expect = 9e-83, Method: Compositional matrix adjust. Identities = 172/454 (37%), Positives = 271/454 (59%), Gaps = 4/454 (0%) Query: 1 MSTLNEQLGSEPIRKLLLKFSIPAIAGMLVSSLYNLLDRIFVGR-LGALAMTGIG---LT 56 M +E LG E ++KLL K S P I GM+V +LYN++D +FVGR GA ++ IG + Sbjct: 1 MEEKSEFLGKESVKKLLFKLSTPVIIGMIVQALYNVVDTLFVGRAYGAESVQAIGGLSIA 60 Query: 57 LPFMTLLTAFTSLVGIGTSAIVSMRLGQNRKEDAEKVLGNAFSMLTVLMLVVTAIGLIFK 116 P ++ AF ++G G S+I+S LG E AE+ LGN FS+ L +++ L++ Sbjct: 61 FPIQMIIMAFGIVLGTGGSSIISRALGAKENEKAERTLGNVFSLSLFLSILIAVPFLLYL 120 Query: 117 EPILTLLGASELTMGYASDYITIILIGSVFQGIGAGLSNVIRAEGHPAKATIVLVIGTVL 176 PIL + GA+ + YAS+Y+ I+ G +F G + +++RAEG+ A ++IG + Sbjct: 121 GPILEIFGATPGILPYASEYLEYIIEGGIFFVFGVAVQHIVRAEGNARLAMNAMLIGAGI 180 Query: 177 DMILNPLFIFTFNMGIAGAAWTTVLSATVTTILVIAHFVGKKSVLKLRIVNLKLSLPIVK 236 +++L+PLFIF F MG+ GAA TV+S V++ ++ + + K + + NLK L I+K Sbjct: 181 NILLDPLFIFGFEMGVKGAAIATVISQAVSSGWLLHYCLKGKGAVNFKSGNLKPDLKIIK 240 Query: 237 DILSIGLSPFFMQISAFIVGIISNNALKTYGGDVAIGAMTVVNAVMILFLMSAMGITQGA 296 +I +IGL F MQ S+ ++ I NAL +GGDVAI +V V + +G++ G Sbjct: 241 EIGAIGLGSFVMQASSSLMMIFVYNALANHGGDVAIAVFGIVIKVNSFIFLPLLGMSFGL 300 Query: 297 QPILGFNYGAQQYDRVKKTLKLELALVTAICVLTFLMVQIFPTGLARILSNDLELINAAS 356 QPI+GFNYGA++++R+ + +KL L T+ +L L + +F L + S D E + Sbjct: 301 QPIIGFNYGAKKFERIVEAVKLALIATTSFGLLGLLTIYLFTEQLLVLFSADPEYLAVGK 360 Query: 357 HGMKIFLLMLPIIGAQIVGASYFQAVGAAKKAMFLGLLRQVITLIPMLIILPNFFGLTGV 416 H + I L+ +P+IG +V + FQA+G AK + L L RQ++ LIP+++ILPN++GL GV Sbjct: 361 HAVMIMLMGMPLIGLNVVTVTLFQALGQAKPSFLLSLSRQLLFLIPLVLILPNYYGLDGV 420 Query: 417 WAAAPTSDFISCSVAIVFVAREIKRLGKAEQHQA 450 WAA PTSD +S ++ + R + K H Sbjct: 421 WAAYPTSDLLSFILSAFMLFRVYRTFKKHAAHSG 454 >NMA2050 [C] COG0282 Acetate kinase Length = 468 Score = 305 bits (781), Expect = 9e-83, Method: Compositional matrix adjust. Identities = 172/454 (37%), Positives = 271/454 (59%), Gaps = 4/454 (0%) Query: 1 MSTLNEQLGSEPIRKLLLKFSIPAIAGMLVSSLYNLLDRIFVGR-LGALAMTGIG---LT 56 M +E LG E ++KLL K S P I GM+V +LYN++D +FVGR GA ++ IG + Sbjct: 1 MEEKSEFLGKESVKKLLFKLSTPVIIGMIVQALYNVVDTLFVGRAYGAESVQAIGGLSIA 60 Query: 57 LPFMTLLTAFTSLVGIGTSAIVSMRLGQNRKEDAEKVLGNAFSMLTVLMLVVTAIGLIFK 116 P ++ AF ++G G S+I+S LG E AE+ LGN FS+ L +++ L++ Sbjct: 61 FPIQMIIMAFGIVLGTGGSSIISRALGAKENEKAERTLGNVFSLSLFLSILIAVPFLLYL 120 Query: 117 EPILTLLGASELTMGYASDYITIILIGSVFQGIGAGLSNVIRAEGHPAKATIVLVIGTVL 176 PIL + GA+ + YAS+Y+ I+ G +F G + +++RAEG+ A ++IG + Sbjct: 121 GPILEIFGATPGILPYASEYLEYIIEGGIFFVFGVAVQHIVRAEGNARLAMNAMLIGAGI 180 Query: 177 DMILNPLFIFTFNMGIAGAAWTTVLSATVTTILVIAHFVGKKSVLKLRIVNLKLSLPIVK 236 +++L+PLFIF F MG+ GAA TV+S V++ ++ + + K + + NLK L I+K Sbjct: 181 NILLDPLFIFGFEMGVKGAAIATVISQAVSSGWLLHYCLKGKGAVNFKSGNLKPDLKIIK 240 Query: 237 DILSIGLSPFFMQISAFIVGIISNNALKTYGGDVAIGAMTVVNAVMILFLMSAMGITQGA 296 +I +IGL F MQ S+ ++ I NAL +GGDVAI +V V + +G++ G Sbjct: 241 EIGAIGLGSFVMQASSSLMMIFVYNALANHGGDVAIAVFGIVIKVNSFIFLPLLGMSFGL 300 Query: 297 QPILGFNYGAQQYDRVKKTLKLELALVTAICVLTFLMVQIFPTGLARILSNDLELINAAS 356 QPI+GFNYGA++++R+ + +KL L T+ +L L + +F L + S D E + Sbjct: 301 QPIIGFNYGAKKFERIVEAVKLALIATTSFGLLGLLTIYLFTEQLLVLFSADPEYLAVGK 360 Query: 357 HGMKIFLLMLPIIGAQIVGASYFQAVGAAKKAMFLGLLRQVITLIPMLIILPNFFGLTGV 416 H + I L+ +P+IG +V + FQA+G AK + L L RQ++ LIP+++ILPN++GL GV Sbjct: 361 HAVMIMLMGMPLIGLNVVTVTLFQALGQAKPSFLLSLSRQLLFLIPLVLILPNYYGLDGV 420 Query: 417 WAAAPTSDFISCSVAIVFVAREIKRLGKAEQHQA 450 WAA PTSD +S ++ + R + K H Sbjct: 421 WAAYPTSDLLSFILSAFMLFRVYRTFKKHAAHSG 454 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.327 0.141 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 30,241,668 Number of extensions: 1246857 Number of successful extensions: 4955 Number of sequences better than 1.0e-05: 99 Number of HSP's gapped: 4768 Number of HSP's successfully gapped: 100 Length of query: 459 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 349 Effective length of database: 25,691,691 Effective search space: 8966400159 Effective search space used: 8966400159 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 117 (49.7 bits)