BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0386.0029.p (314 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PH1048 [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) 207 2e-53 VNG0096C [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) 108 9e-24 DR0585 [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) 66 7e-11 BH3246 [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) 65 2e-10 PH1046 [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) 57 6e-08 >PH1048 [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) Length = 320 Score = 207 bits (526), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 115/306 (37%), Positives = 170/306 (55%), Gaps = 5/306 (1%) Query: 13 LKDCLGLGSHEQLVVVADDDKRELAESVYEAGKRLGAETVLMIMKERTRSGXXXXXXXXX 72 LK C+G+ E ++V+AD+ +RE+ ++ K LGAE + + + R G Sbjct: 15 LKQCMGVKPGETVLVIADEPEREIGYHLWMKAKELGAEAIYVEILPRKMHGEEPPKPVAE 74 Query: 73 XXXKSDVAVCITAHSMXXXXXXXXXXXXXXXXXXMPGMTDDMFSQGAITADYGQVKVLTE 132 +DV + T+ S+ +PG+T ++F + ++ADY + LT Sbjct: 75 AMKVADVVIAPTSKSITHTLAKKEACERGARVATLPGITREIFVR-TMSADYEGMLELTN 133 Query: 133 RVAALLSGSRSVRIEKD-GLSLSFSTKGREGILSTGLYLNPGESGNLPSGEAYIAPLEGT 191 ++A +L + V I G L FS +GR+ STG+Y NPGE GNLP EAYIAPLEGT Sbjct: 134 KIADVLDKGKEVHILTPLGTDLKFSIEGRKARRSTGIYRNPGECGNLPGAEAYIAPLEGT 193 Query: 192 ASGRIKVDGSVAGIGALSSPMVLTVEQG---RLTXXXXXXXXXXXXXXXXXXXRLLGEFG 248 A+GR+ +DGS+AGIG L P+ + V+ G + R + EFG Sbjct: 194 ANGRVVIDGSMAGIGVLKEPIEIIVKDGFAEDIRGGEEAEKLREILSRYGKEARNIAEFG 253 Query: 249 IGTNNKARITGVVLEDEKVYGTIHVAFGSNNTFGGVVAAGVHIDAVVMKPDVYLDNKLIM 308 +GTN KARI+G +LEDEKV+GT+H+A GSN FGG V A VH+D ++ +P V +D K + Sbjct: 254 VGTNPKARISGNILEDEKVFGTVHIALGSNYDFGGKVKAPVHLDGIIKEPTVLIDGKYLF 313 Query: 309 RGGELL 314 + G L+ Sbjct: 314 KDGNLV 319 >VNG0096C [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) Length = 313 Score = 108 bits (271), Expect = 9e-24, Method: Compositional matrix adjust. Identities = 85/301 (28%), Positives = 133/301 (44%), Gaps = 9/301 (2%) Query: 13 LKDCLGLGSHEQLVVVADDDKRELAESVYEAGKRLGAETVLMIMKERTRSGXXXXXXXXX 72 ++ CLGL E VVV DD + E+ ++YEA + + T ++ + G Sbjct: 11 VEQCLGLHPEESCVVVTDDKREEIGRAIYEAARDVSDATTMLQYPPGDQHGEEPPAPVAA 70 Query: 73 XXXKSDVAVCITAHSMXXXXXXXXXXXXXXXXXXMPGMTDDMFSQGAITADYGQVKVLTE 132 +DV V T S+ +PG+T D+F+ G + ADY ++ E Sbjct: 71 AMADADVFVAPTTKSLSHTRARGAANDAGARGATLPGITADVFTTG-LDADYETIEAHCE 129 Query: 133 RVAALLSGSRSVRIEKD-GLSLSFSTKGREGILSTGLYLNPGESGNLPSGEAYIAPLEGT 191 V A ++ + VR+ G ++F R TG+ GE NLP+GE +++P + Sbjct: 130 DVLAQVADATEVRVTTPLGTDITFEAGDRAWRDDTGIVREGGEFSNLPAGEVFVSPTD-- 187 Query: 192 ASGRIKVDGSVAGIGALSS-PMVLTVEQGRLTXXXXXXXXXXXXXXXX---XXXRLLGEF 247 A+G VDG++ G L + V G +T L E Sbjct: 188 ANGTYVVDGTMMPHGKLDDEELRFEVADGVVTEISDDRVREQVEAGSEEVGEDAYNLAEL 247 Query: 248 GIGTNNK-ARITGVVLEDEKVYGTIHVAFGSNNTFGGVVAAGVHIDAVVMKPDVYLDNKL 306 GIGTN + G VL DEK GT+H+A G + GG A +H+D ++ +P VY D + Sbjct: 248 GIGTNVAVTELVGSVLLDEKAAGTVHIAIGDDAGIGGDTDAPLHLDGIIREPTVYADGEE 307 Query: 307 I 307 + Sbjct: 308 V 308 >DR0585 [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) Length = 380 Score = 66.2 bits (160), Expect = 7e-11, Method: Compositional matrix adjust. Identities = 57/178 (32%), Positives = 78/178 (43%), Gaps = 11/178 (6%) Query: 122 ADYGQVKVLTERVAALLSGSRSVRIEKDGLSLSFSTKGREGILSTGLYLNPGESGNLPSG 181 A +G+V+ L R+ LS + VRIE G L+ GR ++ N N+PSG Sbjct: 182 AAWGEVRELQARLIERLSRADVVRIEAPGTDLTLRVGGR-------IWSNSDGKRNMPSG 234 Query: 182 EAYIAPLEGTASGRI--KVDGSVAGIGALSSPMVLTVEQGRLTXXXXXXXXXXXXXXXXX 239 E + PLE +A G + V S G + +V Q Sbjct: 235 EVFTGPLEDSAEGVVTFTVPASYQGQTVRGARLVFRAGQVVEASAEEGEAVLHAALDTDP 294 Query: 240 XXRLLGEFGIGTN-NKARITGVVLEDEKVYGTIHVAFG-SNNTFGGVVAAGVHIDAVV 295 R LGE GIGTN R G +L DEK+ GT+H+A G S GG+ + VH D + Sbjct: 295 GARRLGELGIGTNFGIQRGIGNILFDEKIGGTVHLAIGKSYPETGGLNRSAVHWDLIT 352 >BH3246 [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) Length = 371 Score = 64.7 bits (156), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 52/210 (24%), Positives = 88/210 (41%), Gaps = 18/210 (8%) Query: 115 FSQGAITADYGQVKVLTERVAALLSGSRSVRIEKDGLSLSFSTKGREGILSTGLYLNPGE 174 F DYG++ + + AL++ + V+I+ G L+FS KG + G Sbjct: 157 FYFNVCNLDYGKMSHAMDALVALMNRTDKVQIKGPGTDLTFSIKGIPAVKCAG------- 209 Query: 175 SGNLPSGEAYIAPLEGTASGRIKVDGSVAGIGALSSPMVLTVEQGRLTXXXXXXXXXXXX 234 N+P GE Y AP+ + +G I + G + T + G++ Sbjct: 210 EMNIPDGEVYTAPVRDSVNGTISYNTPTPYQGFTFEQVSFTFKDGKIIEATANDTERINR 269 Query: 235 XXXXXX-XRLLGEFGIGTNNKA-RITGVVLEDEKVYGTIHVAFGS--NNTFGGVVAAGVH 290 R +GEF IG N +L DEK+ G+ H G ++ F G + +H Sbjct: 270 ILDSDEGARYIGEFAIGVNPYILHPMKDILFDEKIDGSFHFTPGQCYDDAFNG-NHSTIH 328 Query: 291 IDAV-VMKP-----DVYLDNKLIMRGGELL 314 D V + +P ++Y D++LI + G + Sbjct: 329 WDIVMIQRPEYGGGEIYFDDQLIRKDGRFV 358 >PH1046 [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) Length = 350 Score = 56.6 bits (135), Expect = 6e-08, Method: Compositional matrix adjust. Identities = 55/218 (25%), Positives = 88/218 (40%), Gaps = 14/218 (6%) Query: 109 GMTDDMFSQGAITADYGQVKVLTERVAALLSGSRSVRIEKD-GLSLSFSTKGREGILSTG 167 GM DM + D ++ E +A + + VRI G + F ++ Sbjct: 120 GMNPDMMVRTIGKVDMKKLSKFLEMIAEITQRGKHVRITTPAGTDVEFENHPERPLIVHS 179 Query: 168 LYLNPGESGNLPSGEAYIAPLEGTASGRIKVDGSV-AGIGALSSPMVLTVEQGRLTXXX- 225 Y+ G LP ++ LE + +G I DGSV IG L P+ L VE+G++ Sbjct: 180 GYVPKGTYEMLPGQISWTPKLE-SINGVIVFDGSVYPPIGLLREPIKLEVERGKIVNIEG 238 Query: 226 ---XXXXXXXXXXXXXXXXRLLGEFGIGTNNKARITGVVLEDEKVYGTIHVAFGS----- 277 L G N A++TG ++EDE+V+G G+ Sbjct: 239 GREAREFSSWLEGFNDPNMYQLAHISYGFNPGAKLTGNIVEDERVWGATEWGIGNIGPRL 298 Query: 278 -NNTFGGVVAAGVHIDAVVMKPDVYLDNKLIMRGGELL 314 + GG+ AA H D + + V++D IM G ++ Sbjct: 299 VPDIPGGIPAAS-HTDGICLNSSVWIDGVQIMDEGNIV 335 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.134 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,215,791 Number of extensions: 715588 Number of successful extensions: 1902 Number of sequences better than 1.0e-05: 7 Number of HSP's gapped: 1899 Number of HSP's successfully gapped: 8 Length of query: 314 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 208 Effective length of database: 26,153,027 Effective search space: 5439829616 Effective search space used: 5439829616 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 116 (49.3 bits)