BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0386.0026.p (385 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2267 [V] COG1680 Beta-lactamase class C and other penicillin b... 378 e-105 BS_pbpE [V] COG1680 Beta-lactamase class C and other penicillin ... 106 6e-23 alr0153 [V] COG1680 Beta-lactamase class C and other penicillin ... 92 1e-18 Rv1922 [V] COG1680 Beta-lactamase class C and other penicillin b... 82 1e-15 MT1973 [V] COG1680 Beta-lactamase class C and other penicillin b... 82 1e-15 >BH2267 [V] COG1680 Beta-lactamase class C and other penicillin binding proteins Length = 252 Score = 378 bits (970), Expect = e-105, Method: Compositional matrix adjust. Identities = 0/334 (0%), Positives = 0/334 (0%), Gaps = 9/334 (2%) Query: 43 QEQLHTIIQTEAEATGFSGTILVKSGTHAAVSGAYGYASKADTRPNRPDTRYGHASGSKL 102 + ++ + Y + P + Sbjct: 2 SDWNPSLY----LHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAAR 57 Query: 103 FTAIAVCQLMEQGKLALDSKIMDILEQAEFPRFSSDITVHHLLTHSSGIPDYFDEETMDD 162 T I M + + V L + + + + Sbjct: 58 ITGIDSSPAMIAEARSALPDCQFVEA-DIRN----WQPVQALDLIFANASLQWLPDHYEL 112 Query: 163 FASLWTTRPMYQLRELKDFLPMFQELPMKFTPGTRFHYNNAGYILLGLLVEKLGGMAFTD 222 F L + + ++ + + V + Sbjct: 113 FPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEA 172 Query: 223 YVEQFIFKASGMADSGYFSLDALPTNTAHGYIENEDGTWTTNIYSIPVKGGADGGAFVTA 282 E I++ + +D T + + + Sbjct: 173 GCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQ 232 Query: 283 PDMLLLWEGLLQHKXXXXXXXXXXXXXHIHDDKEEYYGYGVWITKRGGGIYKYHVMGYDP 342 Sbjct: 233 292 Query: 343 GVSFHSAYYPASGVTTAVLCNHSRGAYRMMRAIE 376 Sbjct: 293 326 >BS_pbpE [V] COG1680 Beta-lactamase class C and other penicillin binding proteins Length = 252 Score = 106 bits (265), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 9/216 (4%), Positives = 23/216 (10%), Gaps = 15/216 (6%) Query: 43 QEQLHTIIQTEAEATGFSGTILVKSGTHAAVSGAYGYASKADTRPNRPDTRYGHASGSKL 102 + + G + R S Sbjct: 6 PSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITGIDSSP 65 Query: 103 FTAIAVCQLMEQGKLALDSKIMDILEQAEFPRFS-SDITVHHLLTHSSGIPDYFDEETMD 161 + + + L + Sbjct: 66 AMIAEARSALPDCQFVEADIRNWQP-----VQALDLIFANASLQWLPDHYELF-----PH 115 Query: 162 DFASLWTTRPMYQLRELKDFLPMFQELPMKFTPGTRFHYNNAGYILLGLLVEKLGGMAFT 221 + L + L + + + + A Sbjct: 116 LVSLLNPQGVLAVQMPDNWLE-PTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGC 174 Query: 222 DYVEQFIFKASGMADSGYFSLDALPTNTAH---GYI 254 + M T Sbjct: 175 EVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLT 210 >alr0153 [V] COG1680 Beta-lactamase class C and other penicillin binding proteins Length = 252 Score = 92.4 bits (228), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 9/222 (4%), Positives = 32/222 (14%), Gaps = 11/222 (4%) Query: 76 AYGYASKADTRPNRPDTRYGHASGSKLFTAIAVCQLMEQGKLALDSKIMDILEQAEFPRF 135 G + R S + + + Sbjct: 39 GCGPGNSTALLQQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQP--VQALDL 96 Query: 136 SSDITVHHLLTHSSGIPDYFDEETMDDFASLWTTRPMYQLRELKDFLPMFQELPMKFTPG 195 L + + L+ + +E+ + Sbjct: 97 IFANASLQWLPDHYELFPHL---VSLLNPQGVLAVQ-MPDNWLEPTHVLMREV--AWEQN 150 Query: 196 TRFHYNNAGYILLGLLVEKLGGMAFTDYVEQFIFKASGMADSGYF-SLDALPTNTAHGYI 254 + D + + Sbjct: 151 YPDRGREPLAGVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLT 210 Query: 255 ENEDGTWTTNIYSIPVKGGADGGAFVTAPDMLLLWEGLLQHK 296 E+E + + + + + ++ + Sbjct: 211 ESEQQLFLKRYHQMLEEQ--YPLQENGQILLAFPRLFIVARR 250 >Rv1922 [V] COG1680 Beta-lactamase class C and other penicillin binding proteins Length = 252 Score = 82.4 bits (202), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 4/166 (2%), Positives = 20/166 (12%), Gaps = 16/166 (9%) Query: 74 SGAYGYASKADTRPNRPDTRYGHASGSKLFTAIAVCQLMEQGKLALDSKIMDILEQAEFP 133 + + + L +L L+ + + ++ Sbjct: 75 LPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLLNPQGVLAVQ--MPD 132 Query: 134 RFSSDITVHHLLTHSSGIPDYFDEETMDDFASLWTTRPMYQLR----ELKDFLPMFQELP 189 + V Sbjct: 133 NWLEPTHVLMREVAWEQNYPDR---------GREPLA-GVHAYYDILSEAGCEVDIWRTT 182 Query: 190 MKFTPGTRFHYNNAGYILLGLLVEKLGGMAFTDYVEQFIFKASGMA 235 + + + + + + Sbjct: 183 YYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQ 228 >MT1973 [V] COG1680 Beta-lactamase class C and other penicillin binding proteins Length = 252 Score = 82.4 bits (202), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 4/166 (2%), Positives = 20/166 (12%), Gaps = 16/166 (9%) Query: 74 SGAYGYASKADTRPNRPDTRYGHASGSKLFTAIAVCQLMEQGKLALDSKIMDILEQAEFP 133 + + + L +L L+ + + ++ Sbjct: 75 LPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLLNPQGVLAVQ--MPD 132 Query: 134 RFSSDITVHHLLTHSSGIPDYFDEETMDDFASLWTTRPMYQLR----ELKDFLPMFQELP 189 + V Sbjct: 133 NWLEPTHVLMREVAWEQNYPDR---------GREPLA-GVHAYYDILSEAGCEVDIWRTT 182 Query: 190 MKFTPGTRFHYNNAGYILLGLLVEKLGGMAFTDYVEQFIFKASGMA 235 + + + + + + Sbjct: 183 YYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQ 228 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.135 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 29,524,359 Number of extensions: 1251345 Number of successful extensions: 1744 Number of sequences better than 1.0e-05: 35 Number of HSP's gapped: 1730 Number of HSP's successfully gapped: 35 Length of query: 385 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 277 Effective length of database: 25,922,359 Effective search space: 7180493443 Effective search space used: 7180493443 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)