BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0386.0023.p (291 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1532 [F] COG0005 Purine nucleoside phosphorylase 318 6e-87 BH1531 [F] COG0005 Purine nucleoside phosphorylase 287 2e-77 TM1737 [F] COG0005 Purine nucleoside phosphorylase 259 3e-69 TM1596 [F] COG0005 Purine nucleoside phosphorylase 216 4e-56 SPAC1805.16c [F] COG0005 Purine nucleoside phosphorylase 195 6e-50 >BH1532 [F] COG0005 Purine nucleoside phosphorylase Length = 275 Score = 318 bits (815), Expect = 6e-87, Method: Compositional matrix adjust. Identities = 158/275 (57%), Positives = 201/275 (73%), Gaps = 7/275 (2%) Query: 16 QVKEAAEYIQAKLGAYAPTXXXXXXXXXXXXXXQIEDAVYLPYEEIPHFPRSTVEGHAGR 75 +VK++AEY+ K+ P +IE+AV++PYE+IP+FP STVEGHAG+ Sbjct: 7 KVKQSAEYLLGKI-KNKPAIGLILGSGLGELANEIEEAVHIPYEQIPNFPVSTVEGHAGQ 65 Query: 76 FVIGKLEGKDVMIMQGRFHYYEGYAMRKVVLPVYVMAKLGIGTLVITNAGGGMNRSFKAG 135 VIG L GK+V+ MQGRFHYYEGY M++V PV VM ++G+ +V+TNA GGMN++F G Sbjct: 66 LVIGTLHGKNVVAMQGRFHYYEGYTMQEVTFPVRVMKEIGVELIVVTNACGGMNKNFAPG 125 Query: 136 DLMLITDHLNMTGDNPLIGPNDPELGVRFPDMSRAYDPEYIALAKKLAGEVKGVNGETLV 195 DLM+ITDHLNMTGDNPLIGPN E G RFPDMS AY PE + ++ A + + Sbjct: 126 DLMIITDHLNMTGDNPLIGPNVEEWGPRFPDMSHAYTPELVEFVEETANRLD------IK 179 Query: 196 LQEGVYAGISGPTYETPAELKMLAALGGDAVGMSTVPEVIVARHSRLRVLGITCITDMAI 255 +Q+GVYAGI+GPTY T AEL ML LGGD +GMSTVPEVIVARH+ ++V+GI+CITDMAI Sbjct: 180 VQKGVYAGITGPTYMTGAELIMLRNLGGDVIGMSTVPEVIVARHAGMKVIGISCITDMAI 239 Query: 256 GDELEPLTHEQVVQVANLTKPKFIGLVRAFVREVQ 290 G+E+ +THE+VV VAN TKPKFI LV+ V V Sbjct: 240 GEEIAGITHEEVVAVANKTKPKFIKLVKEIVANVN 274 >BH1531 [F] COG0005 Purine nucleoside phosphorylase Length = 272 Score = 287 bits (734), Expect = 2e-77, Method: Compositional matrix adjust. Identities = 144/274 (52%), Positives = 187/274 (68%), Gaps = 7/274 (2%) Query: 16 QVKEAAEYIQAKLGAYAPTXXXXXXXXXXXXXXQIEDAVYLPYEEIPHFPRSTVEGHAGR 75 Q++EA +IQ ++ PT +IE V +PY +IPHFP STV+GHAG+ Sbjct: 6 QLQEATTFIQQQI-ETKPTIGLILGSGLGILADEIEQPVKVPYSDIPHFPVSTVQGHAGQ 64 Query: 76 FVIGKLEGKDVMIMQGRFHYYEGYAMRKVVLPVYVMAKLGIGTLVITNAGGGMNRSFKAG 135 VIG LEGK V+ MQGRFH+YEGY++ V PV VM LG+ +++TNA GG+N SF+AG Sbjct: 65 LVIGMLEGKQVIAMQGRFHFYEGYSLEVVTFPVRVMKALGVEQIIVTNAAGGVNESFEAG 124 Query: 136 DLMLITDHLNMTGDNPLIGPNDPELGVRFPDMSRAYDPEYIALAKKLAGEVKGVNGETLV 195 DLM+I DH+N NPLIGPND GVRFPDMS AY LAK+ KG N L Sbjct: 125 DLMIIRDHINNMAQNPLIGPNDEAFGVRFPDMSNAYSERLRTLAKE-----KG-NTLNLK 178 Query: 196 LQEGVYAGISGPTYETPAELKMLAALGGDAVGMSTVPEVIVARHSRLRVLGITCITDMAI 255 LQEGVY +GP YETPAE++M+ LGGDAVGMSTVPEVIVARH+ L VLGI+CI++MA Sbjct: 179 LQEGVYVANTGPVYETPAEVRMIRKLGGDAVGMSTVPEVIVARHAGLEVLGISCISNMAA 238 Query: 256 GDELEPLTHEQVVQVANLTKPKFIGLVRAFVREV 289 G +PL+H++V++ + F+ LV+A V+++ Sbjct: 239 GILPQPLSHDEVIETTERVRQDFLNLVKAIVKDM 272 >TM1737 [F] COG0005 Purine nucleoside phosphorylase Length = 299 Score = 259 bits (662), Expect = 3e-69, Method: Compositional matrix adjust. Identities = 134/280 (47%), Positives = 181/280 (64%), Gaps = 10/280 (3%) Query: 13 YGMQVKEAAEYIQAKLGAYAPTXXXXXXXXXXXXXXQIED---AVYLPYEEIPHFPRSTV 69 Y +V++A EY++ ++ P ++E + +PY EIP FP ST Sbjct: 27 YKERVEKAVEYLKGQIDE-TPEIAIILGSGLSVIADEVEKEGTSKKIPYSEIPGFPISTA 85 Query: 70 EGHAGRFVIGKLEGKDVMIMQGRFHYYEGYAMRKVVLPVYVMAKLGIGTLVITNAGGGMN 129 GH G + GKL GK+VM+M GRFHYYEGY+M++V P+ VM LG+ L++TNA GG+N Sbjct: 86 PGHKGELIFGKLFGKNVMLMNGRFHYYEGYSMKEVTFPIRVMQLLGVEILIVTNAAGGLN 145 Query: 130 RSFKAGDLMLITDHLNMTGDNPLIGPNDPELGVRFPDMSRAYDPEYIALAKKLAGEVKGV 189 F+ G M+ITDH+N GDNPLIGPN E G RFPDMS YD E I LA A E+ Sbjct: 146 PDFEVGRPMIITDHINFMGDNPLIGPNVDEWGPRFPDMSEPYDKELIELAYNSAREL--- 202 Query: 190 NGETLVLQEGVYAGISGPTYETPAELKMLAALGGDAVGMSTVPEVIVARHSRLRVLGITC 249 + + +GVY ++GP +ETPAEL+ML G DAVGMSTVPEVIVARH ++RVLGI+ Sbjct: 203 ---GIPVYQGVYVAVTGPCFETPAELRMLRKFGADAVGMSTVPEVIVARHGQIRVLGISA 259 Query: 250 ITDMAIGDELEPLTHEQVVQVANLTKPKFIGLVRAFVREV 289 ITD A+ ++L+PLT E+V++VA T K ++ VR++ Sbjct: 260 ITDRAVPEDLKPLTAEEVLEVAEKTGRKIAQIIFEVVRKL 299 >TM1596 [F] COG0005 Purine nucleoside phosphorylase Length = 265 Score = 216 bits (549), Expect = 4e-56, Method: Compositional matrix adjust. Identities = 109/229 (47%), Positives = 153/229 (66%), Gaps = 11/229 (4%) Query: 49 QIEDAVYLPYEEIPHFPRSTVEGHAGRFVIGKLEGKDVMIMQGRFHYYEGYAMRKVVLPV 108 ++ED V + Y++IPHFP+ TVEGH+G+ V G++ K VMIM GRFH YEG+ V PV Sbjct: 36 KVEDPVIIDYKDIPHFPQPTVEGHSGKLVFGRISDKPVMIMAGRFHLYEGHDPATVAFPV 95 Query: 109 YVMAKLGIGTLVITNAGGGMNRSFKAGDLMLITDHLNMTGDNPLIGPNDPELGVRFPDMS 168 Y+ +G+ +V+TNA G +N FK G+++L+ D +N NPL GPND ++G RFPDMS Sbjct: 96 YLAKYVGVKGVVVTNAAGAINPEFKPGEIILVRDIINFMFRNPLRGPNDEKIGPRFPDMS 155 Query: 169 RAYDPEYIALAKKLAGEVKGVNGETLVLQEGVYAGISGPTYETPAELKMLAALGGDAVGM 228 DPE+ A+K+ E L L+EGVY G+ GP+YETPAE+++ LG D VGM Sbjct: 156 SVVDPEW---ARKIQ--------ERLSLKEGVYIGVLGPSYETPAEIRVFEKLGADLVGM 204 Query: 229 STVPEVIVARHSRLRVLGITCITDMAIGDELEPLTHEQVVQVANLTKPK 277 STVPEVI A+H L+V+ +C+T+MA G L+HE+VV+ + + K Sbjct: 205 STVPEVIAAKHCGLKVVVFSCVTNMAAGITHGRLSHEEVVRTTKMAQGK 253 >SPAC1805.16c [F] COG0005 Purine nucleoside phosphorylase Length = 315 Score = 195 bits (496), Expect = 6e-50, Method: Compositional matrix adjust. Identities = 112/261 (42%), Positives = 149/261 (57%), Gaps = 13/261 (4%) Query: 6 QQHANTPYGMQVKEAAEYI--QAKLGAYAPTXXXXXXXXXXXXXXQIEDAVY-LPYEEIP 62 Q H PY ++ EA EYI Q P + VY +PYE+IP Sbjct: 13 QPHHTEPY-IKALEAREYIIEQVPEELSKPKVAIICGSGLGTLASGLSAPVYEVPYEDIP 71 Query: 63 HFPRSTVEGHAGRFVIGKLEGKDV--MIMQGRFHYYEGYAMRKVVLPVYVMAKLGIGTLV 120 HF S V GHA + L K V MI+ GR+H YEGY + PV +M +G+ +V Sbjct: 72 HFHVSHVPGHASKLYFAFLGEKRVPTMILAGRYHSYEGYPIEATTFPVRLMKVMGVEVMV 131 Query: 121 ITNAGGGMNRSFKAGDLMLITDHLN---MTGDNPLIGPNDPELGVRFPDMSRAYDPEYIA 177 +TNA GG+N+ FK GDLM++ DH+N + G NPL GPN E GVRFP +S AYD E Sbjct: 132 VTNAAGGLNQGFKVGDLMILKDHINFPGLAGMNPLRGPNAHEFGVRFPPLSDAYDLEL-- 189 Query: 178 LAKKLAGEVKGVNGETLVLQEGVYAGISGPTYETPAELKMLAALGGDAVGMSTVPEVIVA 237 +KL + + + + EG YA +SGP +ET AE +MLA +G D VGMSTVPEV+VA Sbjct: 190 --RKLVYDAAKAHKVSRTIHEGCYAFVSGPCFETRAESRMLALMGADCVGMSTVPEVVVA 247 Query: 238 RHSRLRVLGITCITDMAIGDE 258 RH +RVL I+ +T+ + +E Sbjct: 248 RHCGIRVLAISLVTNNVVVEE 268 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.138 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,479,781 Number of extensions: 954955 Number of successful extensions: 1625 Number of sequences better than 1.0e-05: 17 Number of HSP's gapped: 1597 Number of HSP's successfully gapped: 17 Length of query: 291 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 186 Effective length of database: 26,268,361 Effective search space: 4885915146 Effective search space used: 4885915146 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)