BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0386.0022.p (274 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1531 [F] COG0005 Purine nucleoside phosphorylase 323 2e-88 BH1532 [F] COG0005 Purine nucleoside phosphorylase 274 1e-73 TM1737 [F] COG0005 Purine nucleoside phosphorylase 245 5e-65 TM1596 [F] COG0005 Purine nucleoside phosphorylase 225 6e-59 SPAC1805.16c [F] COG0005 Purine nucleoside phosphorylase 192 4e-49 >BH1531 [F] COG0005 Purine nucleoside phosphorylase Length = 272 Score = 323 bits (828), Expect = 2e-88, Method: Compositional matrix adjust. Identities = 155/268 (57%), Positives = 189/268 (70%) Query: 7 SNIQEAAVYIKDKCPVAPEXXXXXXXXXXXXADLITDEVVIPYHEIPHFPVSTVEGHEGE 66 + +QEA +I+ + P AD I V +PY +IPHFPVSTV+GH G+ Sbjct: 5 TQLQEATTFIQQQIETKPTIGLILGSGLGILADEIEQPVKVPYSDIPHFPVSTVQGHAGQ 64 Query: 67 LLIGMIEGRRVVMMKGRFHMYEGYGPETTAFPVRVMKELGVASLLVTNAAGGVNTDFTPG 126 L+IGM+EG++V+ M+GRFH YEGY E FPVRVMK LGV ++VTNAAGGVN F G Sbjct: 65 LVIGMLEGKQVIAMQGRFHFYEGYSLEVVTFPVRVMKALGVEQIIVTNAAGGVNESFEAG 124 Query: 127 DLMLITDHLNLTGRNPLTGPNDNALGVRFPDMSTAYSPRLIAVAKEAAAAQGFEFKEGVY 186 DLM+I DH+N +NPL GPND A GVRFPDMS AYS RL +AKE + +EGVY Sbjct: 125 DLMIIRDHINNMAQNPLIGPNDEAFGVRFPDMSNAYSERLRTLAKEKGNTLNLKLQEGVY 184 Query: 187 AGLLGPCYETPAEIVMLRRQGADAVGMSTVSETIVARHAGIEVLGISCITNMAAGILDQP 246 GP YETPAE+ M+R+ G DAVGMSTV E IVARHAG+EVLGISCI+NMAAGIL QP Sbjct: 185 VANTGPVYETPAEVRMIRKLGGDAVGMSTVPEVIVARHAGLEVLGISCISNMAAGILPQP 244 Query: 247 LNHAEVMETADRVREHFLKLVLALIPKM 274 L+H EV+ET +RVR+ FL LV A++ M Sbjct: 245 LSHDEVIETTERVRQDFLNLVKAIVKDM 272 >BH1532 [F] COG0005 Purine nucleoside phosphorylase Length = 275 Score = 274 bits (700), Expect = 1e-73, Method: Compositional matrix adjust. Identities = 134/266 (50%), Positives = 179/266 (67%) Query: 6 QSNIQEAAVYIKDKCPVAPEXXXXXXXXXXXXADLITDEVVIPYHEIPHFPVSTVEGHEG 65 + ++++A Y+ K P A+ I + V IPY +IP+FPVSTVEGH G Sbjct: 5 REKVKQSAEYLLGKIKNKPAIGLILGSGLGELANEIEEAVHIPYEQIPNFPVSTVEGHAG 64 Query: 66 ELLIGMIEGRRVVMMKGRFHMYEGYGPETTAFPVRVMKELGVASLLVTNAAGGVNTDFTP 125 +L+IG + G+ VV M+GRFH YEGY + FPVRVMKE+GV ++VTNA GG+N +F P Sbjct: 65 QLVIGTLHGKNVVAMQGRFHYYEGYTMQEVTFPVRVMKEIGVELIVVTNACGGMNKNFAP 124 Query: 126 GDLMLITDHLNLTGRNPLTGPNDNALGVRFPDMSTAYSPRLIAVAKEAAAAQGFEFKEGV 185 GDLM+ITDHLN+TG NPL GPN G RFPDMS AY+P L+ +E A + ++GV Sbjct: 125 GDLMIITDHLNMTGDNPLIGPNVEEWGPRFPDMSHAYTPELVEFVEETANRLDIKVQKGV 184 Query: 186 YAGLLGPCYETPAEIVMLRRQGADAVGMSTVSETIVARHAGIEVLGISCITNMAAGILDQ 245 YAG+ GP Y T AE++MLR G D +GMSTV E IVARHAG++V+GISCIT+MA G Sbjct: 185 YAGITGPTYMTGAELIMLRNLGGDVIGMSTVPEVIVARHAGMKVIGISCITDMAIGEEIA 244 Query: 246 PLNHAEVMETADRVREHFLKLVLALI 271 + H EV+ A++ + F+KLV ++ Sbjct: 245 GITHEEVVAVANKTKPKFIKLVKEIV 270 >TM1737 [F] COG0005 Purine nucleoside phosphorylase Length = 299 Score = 245 bits (626), Expect = 5e-65, Method: Compositional matrix adjust. Identities = 127/272 (46%), Positives = 167/272 (61%), Gaps = 3/272 (1%) Query: 6 QSNIQEAAVYIKDKCPVAPEXXXXXXXXXXXXADLITDEVV---IPYHEIPHFPVSTVEG 62 + +++A Y+K + PE AD + E IPY EIP FP+ST G Sbjct: 28 KERVEKAVEYLKGQIDETPEIAIILGSGLSVIADEVEKEGTSKKIPYSEIPGFPISTAPG 87 Query: 63 HEGELLIGMIEGRRVVMMKGRFHMYEGYGPETTAFPVRVMKELGVASLLVTNAAGGVNTD 122 H+GEL+ G + G+ V++M GRFH YEGY + FP+RVM+ LGV L+VTNAAGG+N D Sbjct: 88 HKGELIFGKLFGKNVMLMNGRFHYYEGYSMKEVTFPIRVMQLLGVEILIVTNAAGGLNPD 147 Query: 123 FTPGDLMLITDHLNLTGRNPLTGPNDNALGVRFPDMSTAYSPRLIAVAKEAAAAQGFEFK 182 F G M+ITDH+N G NPL GPN + G RFPDMS Y LI +A +A G Sbjct: 148 FEVGRPMIITDHINFMGDNPLIGPNVDEWGPRFPDMSEPYDKELIELAYNSARELGIPVY 207 Query: 183 EGVYAGLLGPCYETPAEIVMLRRQGADAVGMSTVSETIVARHAGIEVLGISCITNMAAGI 242 +GVY + GPC+ETPAE+ MLR+ GADAVGMSTV E IVARH I VLGIS IT+ A Sbjct: 208 QGVYVAVTGPCFETPAELRMLRKFGADAVGMSTVPEVIVARHGQIRVLGISAITDRAVPE 267 Query: 243 LDQPLNHAEVMETADRVREHFLKLVLALIPKM 274 +PL EV+E A++ +++ ++ K+ Sbjct: 268 DLKPLTAEEVLEVAEKTGRKIAQIIFEVVRKL 299 >TM1596 [F] COG0005 Purine nucleoside phosphorylase Length = 265 Score = 225 bits (574), Expect = 6e-59, Method: Compositional matrix adjust. Identities = 116/249 (46%), Positives = 158/249 (63%), Gaps = 5/249 (2%) Query: 7 SNIQEAAVYIKDKCPVAPEXXXXXXXXXXXXADLITDEVVIPYHEIPHFPVSTVEGHEGE 66 I+EA +I ++ ++P+ + + D V+I Y +IPHFP TVEGH G+ Sbjct: 3 KKIEEARTFISERTNLSPDILIILGSGFGPFIEKVEDPVIIDYKDIPHFPQPTVEGHSGK 62 Query: 67 LLIGMIEGRRVVMMKGRFHMYEGYGPETTAFPVRVMKELGVASLLVTNAAGGVNTDFTPG 126 L+ G I + V++M GRFH+YEG+ P T AFPV + K +GV ++VTNAAG +N +F PG Sbjct: 63 LVFGRISDKPVMIMAGRFHLYEGHDPATVAFPVYLAKYVGVKGVVVTNAAGAINPEFKPG 122 Query: 127 DLMLITDHLNLTGRNPLTGPNDNALGVRFPDMSTAYSPRLIAVAKEAAAAQGFEFKEGVY 186 +++L+ D +N RNPL GPND +G RFPDMS+ P +E KEGVY Sbjct: 123 EIILVRDIINFMFRNPLRGPNDEKIGPRFPDMSSVVDPEWARKIQER-----LSLKEGVY 177 Query: 187 AGLLGPCYETPAEIVMLRRQGADAVGMSTVSETIVARHAGIEVLGISCITNMAAGILDQP 246 G+LGP YETPAEI + + GAD VGMSTV E I A+H G++V+ SC+TNMAAGI Sbjct: 178 IGVLGPSYETPAEIRVFEKLGADLVGMSTVPEVIAAKHCGLKVVVFSCVTNMAAGITHGR 237 Query: 247 LNHAEVMET 255 L+H EV+ T Sbjct: 238 LSHEEVVRT 246 >SPAC1805.16c [F] COG0005 Purine nucleoside phosphorylase Length = 315 Score = 192 bits (489), Expect = 4e-49, Method: Compositional matrix adjust. Identities = 102/198 (51%), Positives = 127/198 (64%), Gaps = 7/198 (3%) Query: 47 IPYHEIPHFPVSTVEGHEGELLIGMIEGRRV--VMMKGRFHMYEGYGPETTAFPVRVMKE 104 +PY +IPHF VS V GH +L + +RV +++ GR+H YEGY E T FPVR+MK Sbjct: 65 VPYEDIPHFHVSHVPGHASKLYFAFLGEKRVPTMILAGRYHSYEGYPIEATTFPVRLMKV 124 Query: 105 LGVASLLVTNAAGGVNTDFTPGDLMLITDHLN---LTGRNPLTGPNDNALGVRFPDMSTA 161 +GV ++VTNAAGG+N F GDLM++ DH+N L G NPL GPN + GVRFP +S A Sbjct: 125 MGVEVMVVTNAAGGLNQGFKVGDLMILKDHINFPGLAGMNPLRGPNAHEFGVRFPPLSDA 184 Query: 162 YSPRLIAVAKEAAAAQGFE--FKEGVYAGLLGPCYETPAEIVMLRRQGADAVGMSTVSET 219 Y L + +AA A EG YA + GPC+ET AE ML GAD VGMSTV E Sbjct: 185 YDLELRKLVYDAAKAHKVSRTIHEGCYAFVSGPCFETRAESRMLALMGADCVGMSTVPEV 244 Query: 220 IVARHAGIEVLGISCITN 237 +VARH GI VL IS +TN Sbjct: 245 VVARHCGIRVLAISLVTN 262 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.137 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,505,970 Number of extensions: 908741 Number of successful extensions: 1397 Number of sequences better than 1.0e-05: 18 Number of HSP's gapped: 1376 Number of HSP's successfully gapped: 18 Length of query: 274 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 169 Effective length of database: 26,268,361 Effective search space: 4439353009 Effective search space used: 4439353009 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)