BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0386.0011.p (375 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yqjE [E] COG2195 Di- and tripeptidases 395 e-110 SA1343 [E] COG2195 Di- and tripeptidases 357 1e-98 VC1343 [E] COG2195 Di- and tripeptidases 294 2e-79 lin1412 [E] COG2195 Di- and tripeptidases 134 3e-31 BS_pepT [E] COG2195 Di- and tripeptidases 103 5e-22 >BS_yqjE [E] COG2195 Di- and tripeptidases Length = 134 Score = 395 bits (1015), Expect = e-110, Method: Compositional matrix adjust. Identities = 0/374 (0%), Positives = 0/374 (0%), Gaps = 3/374 (0%) Query: 1 MISQERLIQEFMELVRVDSETGNERQIADVLIEKFGALGLTAIEDDSRERTGHGAGNLFV 60 M ++ + + + + L + + + + Sbjct: 1 MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEKANLFQLLLMLGLTMLF 60 Query: 61 TWPAEEGTAAPKLLFTCHMDTVVPGKNIQPSLGEDGWITSDGSTILGSDDKAGLAALFEA 120 L+ I ++ + G + Sbjct: 61 AAFGLMSL--MVLIIWAVDPQYRLNAMIATTV-VLLLLALIGGIWTLRKSRKSTLLRHTR 117 Query: 121 IRVIQEQKLAHGQIQFVITAGEESGLLGARNMDPSHLDAEFGFALDSNGEVGXXXXXXXX 180 Sbjct: 118 177 Query: 181 XXKVTMQIFGKSAHAGVNPEDGISAIQVASKAISAMKLGRIDNETTANIGKFAGGGPTNV 240 Sbjct: 178 237 Query: 241 VCDHVQLDAEARSIVQEKVELQIASMREALETTAREYGAESEFRSEIIYPAFSFNEHDPV 300 Sbjct: 238 297 Query: 301 VQLAERAITSMGLTPRRFPSGGGSDANVFNGLKVPTVNLAVGYENIHTTKERIKAGDIVK 360 Sbjct: 298 357 Query: 361 VAELVVAIVKESVK 374 Sbjct: 358 371 >SA1343 [E] COG2195 Di- and tripeptidases Length = 377 Score = 357 bits (917), Expect = 1e-98, Method: Compositional matrix adjust. Identities = 182/372 (48%), Positives = 256/372 (68%), Gaps = 4/372 (1%) Query: 1 MISQERLIQEFMELVRVDSETGNERQIADVLIEKFGALGLTAIEDDSRERTGHGAGNLFV 60 MI+++RL+ F+ELV++DSETGNE I +L EKF ALGL ED++ + GA NL Sbjct: 1 MINEQRLLNTFLELVQIDSETGNESTIQPILKEKFIALGLDVKEDEAAKHPKLGANNLVC 60 Query: 61 TWPA--EEGTAAPKLLFTCHMDTVVPGKNIQPSLGEDGWITSDGSTILGSDDKAGLAALF 118 T + EEG PKL T HMDTVVP N++P + +DG+I SDG+TILG+DDKAGLAA+ Sbjct: 61 TMNSTIEEG-EVPKLYLTSHMDTVVPAINVKPIVKDDGYIYSDGTTILGADDKAGLAAML 119 Query: 119 EAIRVIQEQKLAHGQIQFVITAGEESGLLGARNMDPSHLDAEFGFALDSNGEVGXXXXXX 178 E ++VI+EQ++ HGQIQFVIT GEESGL+GA+ ++ LDA+FG+A+D++ +VG Sbjct: 120 EVLQVIKEQQIPHGQIQFVITVGEESGLIGAKELNSELLDADFGYAIDASADVGTTVVGA 179 Query: 179 XXXXKVTMQIFGKSAHAGVNPEDGISAIQVASKAISAMKLGRIDNETTANIGKFAGGGPT 238 ++ +I GK+AHA P++G+SAI +A+KAIS MKLG++D TTANIGKF GG T Sbjct: 180 PTQMLISAKIIGKTAHAS-TPKEGVSAINIAAKAISRMKLGQVDEITTANIGKFHGGSAT 238 Query: 239 NVVCDHVQLDAEARSIVQEKVELQIASMREALETTAREYGAESEFRSEIIYPAFSFNEHD 298 N+V D V L+AEARS E+++ Q+ M + ETTA E G ++E E YP F N+++ Sbjct: 239 NIVADEVILEAEARSHDPERIKTQVKHMTDVFETTASELGGKAEVTVEQSYPGFKINDNE 298 Query: 299 PVVQLAERAITSMGLTPRRFPSGGGSDANVFNGLKVPTVNLAVGYENIHTTKERIKAGDI 358 VV++A+ + ++GL+ SGGGSD ++ N +P+V L VGYE IHTT ER+ + Sbjct: 299 AVVKIAQESARNLGLSANTIISGGGSDGSIINTFGIPSVILGVGYEKIHTTNERMPIKSL 358 Query: 359 VKVAELVVAIVK 370 +A V+ I+K Sbjct: 359 NLLASQVLEIIK 370 >VC1343 [E] COG2195 Di- and tripeptidases Length = 368 Score = 294 bits (752), Expect = 2e-79, Method: Compositional matrix adjust. Identities = 161/367 (43%), Positives = 224/367 (61%), Gaps = 5/367 (1%) Query: 1 MISQERLIQEFMELVRVDSETGNERQIADVLIEKFGALGLTAIEDDSRERTGHGAGNLFV 60 +I+ +RL+ F++L+++DSE+GNE++IA+ L E+ G LG T + +G F Sbjct: 3 LINTQRLVDHFLQLIQIDSESGNEKKIAETLAEQLGELGFTVHKLPVPAEVSNG----FN 58 Query: 61 TWPAEEGTAAPKLLFTCHMDTVVPGKNIQPSLGEDGWITSDGSTILGSDDKAGLAALFEA 120 + EGT +LF+CHMDTV PG I+P + EDG I S G+TILG DDK+G+AA+ EA Sbjct: 59 LYARLEGTLNDSILFSCHMDTVKPGIGIEPVI-EDGIIRSKGNTILGGDDKSGIAAILEA 117 Query: 121 IRVIQEQKLAHGQIQFVITAGEESGLLGARNMDPSHLDAEFGFALDSNGEVGXXXXXXXX 180 +RV+++ + AH I+ T EE GL G+ + D S + AE LD+ G +G Sbjct: 118 VRVLRDSQQAHKTIEIAFTVHEEGGLKGSEHFDMSKVQAEKAIVLDTGGPIGTIVRAAPG 177 Query: 181 XXKVTMQIFGKSAHAGVNPEDGISAIQVASKAISAMKLGRIDNETTANIGKFAGGGPTNV 240 K+ I GK AHAG+ PEDGISAI VA+ AI+ MKL RID ETTANIG GG TN+ Sbjct: 178 QQKIVALIKGKPAHAGLVPEDGISAIAVAADAITQMKLLRIDEETTANIGIVQGGQATNI 237 Query: 241 VCDHVQLDAEARSIVQEKVELQIASMREALETTAREYGAESEFRSEIIYPAFSFNEHDPV 300 V +++ AEARS+ K+E Q+ M E E A ++GA E S Y AF E + Sbjct: 238 VMPELKIVAEARSLNDAKLEAQVQHMIETFERAAEKHGATVEIESTRAYNAFKLEEDNAH 297 Query: 301 VQLAERAITSMGLTPRRFPSGGGSDANVFNGLKVPTVNLAVGYENIHTTKERIKAGDIVK 360 +Q + + ++G+ P+ SGGGSDAN FN + TVNL+ G +HTT+E I D+VK Sbjct: 298 IQAIKASFETIGIEPKTKLSGGGSDANNFNAKGLTTVNLSTGMAKVHTTEEYIAIADMVK 357 Query: 361 VAELVVA 367 +AE V A Sbjct: 358 IAEFVCA 364 >lin1412 [E] COG2195 Di- and tripeptidases Length = 364 Score = 134 bits (336), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 108/370 (29%), Positives = 166/370 (44%), Gaps = 28/370 (7%) Query: 11 FMELVRVDSETGNERQIADVLIEKFGALGLTAIEDDSRERTGHGAGNLFVTWPAEEGTAA 70 F EL+++ S +G E+ + + ++ L + D+ L PA Sbjct: 9 FTELIQIPSVSGKEKAVLTYIKKQITKLNIPYSLDED--------NGLIARIPAT-AVKF 59 Query: 71 PKLLFTCHMDTVVPGKNIQPSLGEDGWITSDGSTILGSDDKAGLAALFEAIRVIQEQKLA 130 P + F H+DT P EDG TS T LG+DDKA +AA+ A+ ++ Sbjct: 60 PTIFFCGHVDTH-PNAASPEFQVEDGIFTSLNGTSLGADDKAAVAAMLAAMDYFSTEETP 118 Query: 131 HGQIQFVITAGEESGLLGARNMDPSHLDAEFGFALDSNGEVGXXXXXXXXXXKVTMQIFG 190 HG+I+F+ T EE G++G R + A +G+ LD+ GEVG V I Sbjct: 119 HGEIEFIFTTKEELGMIGMRLFPEEKITAAYGYCLDAPGEVGNYQLQANTLVAVDFTIAS 178 Query: 191 KSAHAGVNPEDGISAIQVASKAISAMKLGRIDNETTANIGKFAGGGPTNVVCDHVQLDAE 250 A +S I VA A+ A + GRID E I F+GG V D Q DA+ Sbjct: 179 PDA-------TQMSPISVARMALHATRPGRIDRENRWEIQSFSGG-----VNDENQQDAQ 226 Query: 251 ARSIVQEKVELQ-----IASMREALETTAREYGAESEFRSEIIYPAFSFNEHDPVVQLAE 305 + + + I ++RE T +YGA + +IY + + P++ + + Sbjct: 227 LEVLFKSAASFRKALPHIQTIRERFNQTCEKYGATLAEDTRLIYEGYKIHPQHPLMNIFQ 286 Query: 306 RAITSMGLTPRRFPSGGGSDANVFNGLKVPTVNLAVGYENIHTTKERIKAGDIVKVAELV 365 +A L GG+DANV N VPT+ L+ GY + H+ KE I + K+ +LV Sbjct: 287 KAAKKQALKTSEIFLEGGTDANVLNEKGVPTMLLSAGYVHAHSEKETISVEQLEKLTQLV 346 Query: 366 VAIVKESVKG 375 + + ES K Sbjct: 347 IDLA-ESAKN 355 >BS_pepT [E] COG2195 Di- and tripeptidases Length = 408 Score = 103 bits (257), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 26/415 (6%), Positives = 69/415 (16%), Gaps = 58/415 (13%) Query: 4 QERLIQEFMELVRVDSE----------TGNERQIADVLIEKFGALGLTAIEDDSRERTGH 53 + L + + G + + + + + E+ Sbjct: 2 DKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEEMGLI---NVTLSEKGTL 58 Query: 54 GAGNLFVTWPAEEGTAAPKLLFTCHMDTVV--PGKNIQPSLGE--DG------------- 96 + G + + + G Sbjct: 59 ----MATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGDIALGIGDEVLSP 114 Query: 97 ---------------WITSDGSTILGSDDKAGLAALFEAI-RVIQEQKLAHGQIQFVITA 140 A+ + + Sbjct: 115 VMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKKIPHGDIRVAFTPDEE 174 Query: 141 GEESGLLGARNMDPSHLDAEFGFALDSNGEVGXXXXXXXXXXKVTMQIFGKSAHAGVNPE 200 + A GE+ + A Sbjct: 175 VGKGAK-HFDVDAFDARWAYTVDGGGV-GELEFENFNAASVNIKIVGNNVHPGTAKGVMV 232 Query: 201 DGISAIQVASKAISAMKLGRIDNETTANIGKFAGGGPTNVVCDHVQLDAEARSIVQEKVE 260 + +S + A + + A T D + + E + Sbjct: 233 NALSLAARIHAEVPADESPEMTEGYEGFYH-LASMKGTVERADMHYIIRDFDRKQFEARK 291 Query: 261 LQIASMREALETTAREYGAESEFRSEIIYPAFSFNEH-DPVVQL---AERAITSMGLTPR 316 ++ + + + E E Y P + + + Sbjct: 292 RKMMEIAKKVGKGLHPDC-YIELVIEDSYYNMREKVVEHPHILDIAQQAMRDCDIEPELK 350 Query: 317 RFPSGGGSDANVFNGLKVPTVNLAVGYENIHTTKERIKAGDIVKVAELVVAIVKE 371 G F GL P + + ++ + + +A + Sbjct: 351 PIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEKAVQVIVRIAELTA 405 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.133 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 26,281,450 Number of extensions: 1077299 Number of successful extensions: 1833 Number of sequences better than 1.0e-05: 41 Number of HSP's gapped: 1821 Number of HSP's successfully gapped: 41 Length of query: 375 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 267 Effective length of database: 25,922,359 Effective search space: 6921269853 Effective search space used: 6921269853 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 117 (49.7 bits)