BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0386.0009.p (276 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2761 [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase complex... 312 3e-85 BS_bfmBB [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase compl... 306 2e-83 SA1346 [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase complex... 289 3e-78 lin1411 [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase comple... 282 3e-76 mll4300 [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase comple... 189 6e-48 >BH2761 [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes Length = 553 Score = 312 bits (800), Expect = 3e-85, Method: Compositional matrix adjust. Identities = 8/255 (3%), Positives = 38/255 (14%), Gaps = 13/255 (5%) Query: 27 LSETPRIPTIEVEGGRGSSSEYLIDVTPIRNTIATRMRQSVSEIPHAWTMIEVDVTNLVI 86 +++ + L + + I + + + + Sbjct: 180 VTDNADDYRF--------AQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIA 231 Query: 87 LRNKLKDEFKRKEGINLTYLAFLMKAVVSAIKDYPIMNSVWAVDKIIVKRDINISLAVGT 146 N + S + + + + + Sbjct: 232 GGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSS 291 Query: 147 EDSVMTPVIKKADQKNVAGLAREIDELAMKTREGKLRLEDMQGGTFTVNNTGSFGSILSY 206 +++ A + +L + Sbjct: 292 RTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHE 351 Query: 207 PIINYPQAAILTFESIVKKPVVI----NDMIAVRSMANICLSLDHRILDGVICGRFLQRI 262 + + A + N + + + Sbjct: 352 IADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQA 411 Query: 263 KDNVEGYT-PDTKLY 276 ++ Sbjct: 412 AKEIKALIEDSVSRV 426 >BS_bfmBB [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes Length = 553 Score = 306 bits (785), Expect = 2e-83, Method: Compositional matrix adjust. Identities = 19/228 (8%), Positives = 47/228 (20%), Gaps = 1/228 (0%) Query: 50 IDVTPIRNTIATRMRQSVSEIPHAWTMIEVDVTNLVILRNKLKDEFKRKEGINLTYLAFL 109 I + + + + +I R E +L Sbjct: 197 IALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSH 256 Query: 110 MKAVVSAIKDYPIMNSVWAVDKIIVKRDINISLAVGTEDSVMTPVIKKADQKNVAGLARE 169 M+ ++ + S N L+ TE A + + ++ Sbjct: 257 MQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQ 316 Query: 170 IDELAMKTREGKLRLEDMQGGTFTVNNTGSFGSILSYPIINYPQAAILTFESIVKKPVVI 229 + A + + D V + I + I + ++ Sbjct: 317 NADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNIL 376 Query: 230 -NDMIAVRSMANICLSLDHRILDGVICGRFLQRIKDNVEGYTPDTKLY 276 + + A + V + + Sbjct: 377 ALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQAAKEIKALIEDSVS 424 >SA1346 [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes Length = 553 Score = 289 bits (739), Expect = 3e-78, Method: Compositional matrix adjust. Identities = 21/250 (8%), Positives = 55/250 (22%), Gaps = 1/250 (0%) Query: 28 SETPRIPTIEVEGGRGSSSEYLIDVTPIRNTIATRMRQSVSEIPHAWTMIEVDVTNLVIL 87 + + +I + + + + +I Sbjct: 175 LYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGN 234 Query: 88 RNKLKDEFKRKEGINLTYLAFLMKAVVSAIKDYPIMNSVWAVDKIIVKRDINISLAVGTE 147 R E +L M+ ++ + S N L+ TE Sbjct: 235 LANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTE 294 Query: 148 DSVMTPVIKKADQKNVAGLAREIDELAMKTREGKLRLEDMQGGTFTVNNTGSFGSILSYP 207 A + + ++ + A + + D V + Sbjct: 295 QQASALEETAASMEQLTATVKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEIAD 354 Query: 208 IINYPQAAILTFESIVKKPVVINDMIAVRSMANICLSLDHRILDGVICGRFLQRIKDNVE 267 I + I + ++ AV + ++ G + + + E Sbjct: 355 SSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQAAKE 414 Query: 268 GYT-PDTKLY 276 + + Sbjct: 415 IKALIEDSVS 424 >lin1411 [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes Length = 553 Score = 282 bits (722), Expect = 3e-76, Method: Compositional matrix adjust. Identities = 12/236 (5%), Positives = 48/236 (20%), Gaps = 1/236 (0%) Query: 42 RGSSSEYLIDVTPIRNTIATRMRQSVSEIPHAWTMIEVDVTNLVILRNKLKDEFKRKEGI 101 ++ +Y + + + M+ + ++ ++ Sbjct: 181 TDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLT 240 Query: 102 NLTYLAFLMKAVVSAIKDYPIMNSVWAVDKIIVKRDINISLAVGTEDSVMTPVIKKADQK 161 A + + ++V V + + + ++A Sbjct: 241 IDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQASAL 300 Query: 162 NVAGLAREIDELAMKTREGKLRLEDMQGGTFTVNNTGSFGSILSYPIINYPQAAILTFES 221 + E +K R + + + + A + Sbjct: 301 EETAASMEQLTATVKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEIADSSKKIA 360 Query: 222 IVKKPVVINDMIAVRSMANICLSLDHRILDGVICGRFLQRIKDNVEGYTPD-TKLY 276 + + N + G +++ ++ Sbjct: 361 DIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQAAKEIK 416 >mll4300 [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes Length = 301 Score = 189 bits (479), Expect = 6e-48, Method: Compositional matrix adjust. Identities = 0/266 (0%), Positives = 0/266 (0%), Gaps = 7/266 (2%) Query: 7 QVIQEEVQEALEPVRHSGLHLSETPRIPTIEV-----EGGRGSSSEYLIDVTPIRNTIAT 61 I E + + + P + + + +I +R A Sbjct: 152 VPISAETGLNV--DTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAE 209 Query: 62 RMRQSVSEIPHAWTMIEVDVTNLVILRNKLKDEFKRKEGINLTYLAFLMKAVVSAIKDYP 121 EI + ++ + + + K G + + +++ Sbjct: 210 LPYSVTVEIERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMF 269 Query: 122 IMNSVWAVDKIIVKRDINISLAVGTEDSVMTPVIKKADQKNVAGLAREIDELAMKTREGK 181 Sbjct: 270 329 Query: 182 LRLEDMQGGTFTVNNTGSFGSILSYPIINYPQAAILTFESIVKKPVVINDMIAVRSMANI 241 Sbjct: 330 389 Query: 242 CLSLDHRILDGVICGRFLQRIKDNVE 267 Sbjct: 390 415 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,447,652 Number of extensions: 783819 Number of successful extensions: 1551 Number of sequences better than 1.0e-05: 96 Number of HSP's gapped: 1458 Number of HSP's successfully gapped: 96 Length of query: 276 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 171 Effective length of database: 26,268,361 Effective search space: 4491889731 Effective search space used: 4491889731 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)