BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0386.0005.p (260 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_bfmBAB [C] COG0022 Pyruvate/2-oxoglutarate dehydrogenase comp... 362 e-100 BH2762 [C] COG0022 Pyruvate/2-oxoglutarate dehydrogenase complex... 353 1e-97 lin1410 [C] COG0022 Pyruvate/2-oxoglutarate dehydrogenase comple... 353 2e-97 SA1347 [C] COG0022 Pyruvate/2-oxoglutarate dehydrogenase complex... 295 4e-80 SMc03202 [C] COG0022 Pyruvate/2-oxoglutarate dehydrogenase compl... 252 4e-67 >BS_bfmBAB [C] COG0022 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit Length = 553 Score = 362 bits (928), Expect = e-100, Method: Compositional matrix adjust. Identities = 22/251 (8%), Positives = 55/251 (21%), Gaps = 1/251 (0%) Query: 1 MAIMEYIDAIRLAMKEEMERDESVFVLGEDXXXXXXXXXXXXXLQEQFGEERVMDTPLAE 60 M + + + + + + Q + + + T Sbjct: 1 MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLM 60 Query: 61 SAIAGVAIGAAMYGMKPIAEMQYSDFMLPATNQIISEAAKIRYRSNNDWSCPVVIRAPIG 120 +A+ M + Q + + + + A + P+ Sbjct: 61 LQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATS 120 Query: 121 GGIFGGLYHSQCPESIFFGTPGLKIVAPYSAYDAKGLLKAAVRDPDPVLFFENKKCYKLI 180 I + + Y A +G+ A K ++ Sbjct: 121 RNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIV 180 Query: 181 KEDVPEGDYTVPIGEANLLREGSDITVIGYSLPLHFAMQAAEELEREEGITAHILDLRTL 240 ++ + + L + V Y + A+ + A TL Sbjct: 181 TDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAH-IREIAGGNLANTL 239 Query: 241 QPLDREAIIAA 251 R + Sbjct: 240 TIDGRSEMGDL 250 >BH2762 [C] COG0022 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit Length = 553 Score = 353 bits (907), Expect = 1e-97, Method: Compositional matrix adjust. Identities = 22/251 (8%), Positives = 55/251 (21%), Gaps = 1/251 (0%) Query: 1 MAIMEYIDAIRLAMKEEMERDESVFVLGEDXXXXXXXXXXXXXLQEQFGEERVMDTPLAE 60 M + + + + + + Q + + + T Sbjct: 1 MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLM 60 Query: 61 SAIAGVAIGAAMYGMKPIAEMQYSDFMLPATNQIISEAAKIRYRSNNDWSCPVVIRAPIG 120 +A+ M + Q + + + + A + P+ Sbjct: 61 LQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATS 120 Query: 121 GGIFGGLYHSQCPESIFFGTPGLKIVAPYSAYDAKGLLKAAVRDPDPVLFFENKKCYKLI 180 I + + Y A +G+ A K ++ Sbjct: 121 RNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIV 180 Query: 181 KEDVPEGDYTVPIGEANLLREGSDITVIGYSLPLHFAMQAAEELEREEGITAHILDLRTL 240 ++ + + L + V Y + A+ + A TL Sbjct: 181 TDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAH-IREIAGGNLANTL 239 Query: 241 QPLDREAIIAA 251 R + Sbjct: 240 TIDGRSEMGDL 250 >lin1410 [C] COG0022 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit Length = 553 Score = 353 bits (906), Expect = 2e-97, Method: Compositional matrix adjust. Identities = 22/251 (8%), Positives = 55/251 (21%), Gaps = 1/251 (0%) Query: 1 MAIMEYIDAIRLAMKEEMERDESVFVLGEDXXXXXXXXXXXXXLQEQFGEERVMDTPLAE 60 M + + + + + + Q + + + T Sbjct: 1 MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLM 60 Query: 61 SAIAGVAIGAAMYGMKPIAEMQYSDFMLPATNQIISEAAKIRYRSNNDWSCPVVIRAPIG 120 +A+ M + Q + + + + A + P+ Sbjct: 61 LQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATS 120 Query: 121 GGIFGGLYHSQCPESIFFGTPGLKIVAPYSAYDAKGLLKAAVRDPDPVLFFENKKCYKLI 180 I + + Y A +G+ A K ++ Sbjct: 121 RNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIV 180 Query: 181 KEDVPEGDYTVPIGEANLLREGSDITVIGYSLPLHFAMQAAEELEREEGITAHILDLRTL 240 ++ + + L + V Y + A+ + A TL Sbjct: 181 TDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIA-HIREIAGGNLANTL 239 Query: 241 QPLDREAIIAA 251 R + Sbjct: 240 TIDGRSEMGDL 250 >SA1347 [C] COG0022 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit Length = 553 Score = 295 bits (755), Expect = 4e-80, Method: Compositional matrix adjust. Identities = 22/249 (8%), Positives = 55/249 (22%), Gaps = 1/249 (0%) Query: 1 MAIMEYIDAIRLAMKEEMERDESVFVLGEDXXXXXXXXXXXXXLQEQFGEERVMDTPLAE 60 M + + + + + + Q + + + T Sbjct: 1 MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLM 60 Query: 61 SAIAGVAIGAAMYGMKPIAEMQYSDFMLPATNQIISEAAKIRYRSNNDWSCPVVIRAPIG 120 +A+ M + Q + + + + A + P+ Sbjct: 61 LQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATS 120 Query: 121 GGIFGGLYHSQCPESIFFGTPGLKIVAPYSAYDAKGLLKAAVRDPDPVLFFENKKCYKLI 180 I + + Y A +G+ A K ++ Sbjct: 121 RNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIV 180 Query: 181 KEDVPEGDYTVPIGEANLLREGSDITVIGYSLPLHFAMQAAEELEREEGITAHILDLRTL 240 ++ + + L + V Y + A+ + A TL Sbjct: 181 TDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIA-HIREIAGGNLANTL 239 Query: 241 QPLDREAII 249 R + Sbjct: 240 TIDGRSEMG 248 >SMc03202 [C] COG0022 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit Length = 553 Score = 252 bits (643), Expect = 4e-67, Method: Compositional matrix adjust. Identities = 19/276 (6%), Positives = 49/276 (17%), Gaps = 22/276 (7%) Query: 1 MAIMEYIDAIRLAMKEEMERDESVFVLGEDXXXXXXXXXXXXXLQEQFGEERVMDTPLAE 60 M + + + + + + Q + + + T Sbjct: 1 MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLM 60 Query: 61 SAIAGVAIGAAMYGMKPIAEMQYSDFMLPATNQIISEAAKIRYRSNNDWSCPVVIRAPIG 120 +A+ M + Q + + + + A + P+ Sbjct: 61 LQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATS 120 Query: 121 GGIFGGLYHSQCPESIFFGTPGLKIVAPYSAYDAKGLLKAAVRDPDPVLFFENKKCY--- 177 I + + Y A +G+ A K Sbjct: 121 RNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIV 180 Query: 178 -------------KLIKEDVPEGDYTVPIGEANLLREGSDITVIGYSLPLHFAMQAAEEL 224 + V V + +I + + A Sbjct: 181 TDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANT-- 238 Query: 225 EREEGITAHILDLRTLQPLDREAIIAAXXXXRPARC 260 + + + R Sbjct: 239 --LTIDGRSEMGDLAQSVSHMQRSLTD--TVTHVRE 270 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.138 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,325,384 Number of extensions: 782807 Number of successful extensions: 1844 Number of sequences better than 1.0e-05: 33 Number of HSP's gapped: 1786 Number of HSP's successfully gapped: 33 Length of query: 260 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 156 Effective length of database: 26,383,695 Effective search space: 4115856420 Effective search space used: 4115856420 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)