BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0386.0004.p (343 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2763 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase complex... 409 e-114 BS_bfmBAA [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase comp... 399 e-111 lin1409 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase comple... 345 5e-95 BH0213 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase complex... 212 7e-55 lin1044 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase comple... 207 2e-53 >BH2763 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit Length = 553 Score = 409 bits (1050), Expect = e-114, Method: Compositional matrix adjust. Identities = 27/326 (8%), Positives = 59/326 (18%), Gaps = 1/326 (0%) Query: 12 RHKPLGLSDGQVIDMYRTMQLGRKYDERSLLLQRAGKINFHVSGIGQEXXXXXXXXXLDR 71 L + + G + Q++ ++ + E R Sbjct: 5 IRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLMLQTR 64 Query: 72 ENDYFLPYYRDYAFVLSVGMTTRELMLSVFAKAEDPNSGGRQMPGHFGSKRLRIVTGSSP 131 N EL+ S + ++ + + + Sbjct: 65 INLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATSRNID 124 Query: 132 VTTQVPHAVGFALAAKMQKKKFVSFVTFGEGSSNQGDFHEACNFAGVNKLPVIIFCQNNQ 191 + + L + ++ +A ++ +N Sbjct: 125 EKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIVTDNA 184 Query: 192 YAISVPAHKQLGG-KVSDRALGYGFPGVRVDGNDPLEVYRVVKEARERALAGEGPTLIEA 250 + V L + G+R PL T+ Sbjct: 185 DDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLTIDGR 244 Query: 251 MMYRLSPHSTSDNDLAYRTKEEVDANWAKDGIAAFRSYLTELGLWSDEQESDLAAEYNLE 310 S S + + A R S E +A Sbjct: 245 SEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQASALEETA 304 Query: 311 LKAAIEYAENAPFPKPEDTLLHVYSE 336 A + Sbjct: 305 ASMEQLTATVKQNADNARQASQLAQS 330 >BS_bfmBAA [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit Length = 553 Score = 399 bits (1026), Expect = e-111, Method: Compositional matrix adjust. Identities = 27/329 (8%), Positives = 59/329 (17%), Gaps = 1/329 (0%) Query: 9 TINRHKPLGLSDGQVIDMYRTMQLGRKYDERSLLLQRAGKINFHVSGIGQEXXXXXXXXX 68 L + + G + Q++ ++ + E Sbjct: 2 INRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLML 61 Query: 69 LDRENDYFLPYYRDYAFVLSVGMTTRELMLSVFAKAEDPNSGGRQMPGHFGSKRLRIVTG 128 R N EL+ S + ++ + + Sbjct: 62 QTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATSR 121 Query: 129 SSPVTTQVPHAVGFALAAKMQKKKFVSFVTFGEGSSNQGDFHEACNFAGVNKLPVIIFCQ 188 + + + L + ++ +A ++ Sbjct: 122 NIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIVT 181 Query: 189 NNQYAISVPAHKQLGGK-VSDRALGYGFPGVRVDGNDPLEVYRVVKEARERALAGEGPTL 247 +N + V L + G+R PL T+ Sbjct: 182 DNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLTI 241 Query: 248 IEAMMYRLSPHSTSDNDLAYRTKEEVDANWAKDGIAAFRSYLTELGLWSDEQESDLAAEY 307 S S + + A R S E +A Sbjct: 242 DGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQASALE 301 Query: 308 NLELKAAIEYAENAPFPKPEDTLLHVYSE 336 A + Sbjct: 302 ETAASMEQLTATVKQNADNARQASQLAQS 330 >lin1409 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit Length = 553 Score = 345 bits (886), Expect = 5e-95, Method: Compositional matrix adjust. Identities = 23/324 (7%), Positives = 63/324 (19%), Gaps = 1/324 (0%) Query: 14 KPLGLSDGQVIDMYRTMQLGRKYDERSLLLQRAGKINFHVSGIGQEXXXXXXXXXLDREN 73 + ++ ++ + L + K + + ++ L + Sbjct: 4 RIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLMLQT 63 Query: 74 DYFLPYYRDYAFVLSVGMTTRELMLSVFAKAEDPNSGGRQMPGHFGSKRLRIVTGSSPVT 133 L + S + + + + + L + +S Sbjct: 64 RINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATSRNI 123 Query: 134 TQVPHAVGFALAAKMQKKKFVSFVTFGEGSSNQGDFHEACNFAGVNKLPVIIFCQNNQYA 193 + AL + + + + + FA ++ Sbjct: 124 DEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIVTDN 183 Query: 194 ISVPAHKQLGG-KVSDRALGYGFPGVRVDGNDPLEVYRVVKEARERALAGEGPTLIEAMM 252 Q ++ + L + G + Sbjct: 184 ADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLTIDG 243 Query: 253 YRLSPHSTSDNDLAYRTKEEVDANWAKDGIAAFRSYLTELGLWSDEQESDLAAEYNLELK 312 R+ + + + A + +D LE Sbjct: 244 RSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQASALEET 303 Query: 313 AAIEYAENAPFPKPEDTLLHVYSE 336 AA A + D Sbjct: 304 AASMEQLTATVKQNADNARQASQL 327 >BH0213 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit Length = 553 Score = 212 bits (540), Expect = 7e-55, Method: Compositional matrix adjust. Identities = 23/315 (7%), Positives = 56/315 (17%), Gaps = 14/315 (4%) Query: 23 VIDMYRTMQLGRKYDERSLLLQRAGKINFHVSGIGQEXXXXXXXXXLDRENDYFLPYYRD 82 V+ Q G L+LQ ++ + + + D Sbjct: 41 VVSNQLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKV-ELLDSARKTLAQ 99 Query: 83 YAFVLSVGMTTRELMLSVFAKAEDPNSGGRQMPGHFGSKRLRIVTGSSPVTTQVPHAVGF 142 A + L V K + + Sbjct: 100 AATHYKKFKSMAPLPEMVAT----------SRNIDEKYKNYYTALTELIDYLDYGNTGAY 149 Query: 143 ALAAKMQKKKFVSFVTFGEGSSNQGDFHEACNFAGVNKLPVIIFCQNNQYAISVPAHKQL 202 + + S++ + + + + + Sbjct: 150 FAQPTQGMQNAMGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAW 209 Query: 203 GGK-VSDRALGYGFPGVRVDGNDPLEVYRVVKEARERALAGEGPTLIEAMMYRLSPHSTS 261 G + + + R + Sbjct: 210 YGIRRMLLTPLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVR 269 Query: 262 DNDLAYRTKEEVDANWA--KDGIAAFRSYLTELGLWSDEQESDLAAEYNLELKAAIEYAE 319 + A A ++ E S EQ + + + A + A+ Sbjct: 270 EGSDAIYAGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQ 329 Query: 320 NAPFPKPEDTLLHVY 334 +A + Sbjct: 330 SASDTAQHGGKVVDG 344 >lin1044 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit Length = 553 Score = 207 bits (527), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 19/330 (5%), Positives = 62/330 (18%), Gaps = 15/330 (4%) Query: 8 ETINRHKPLGLSDGQVIDMYRTMQLGRKYDERSLLLQRAGKINFHVSGIGQEXXXXXXXX 67 + + + + +L + + Sbjct: 34 HHSQKSFVVSNQLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSA 93 Query: 68 XLDRENDYFLPYYRDYAFVLSVGMTTRELMLSVFAKAEDPNSGGRQMPGHFGSKRLRIVT 127 Y + + M + K + + Sbjct: 94 RKTL-AQAATHYKKFKSMAPLPEMVATSRNI--DEKY---KNYYTALTELIDYLDYGNTG 147 Query: 128 GSSPVTTQVPHAVGFALAAKMQKKKFVSFVTFGEGSSNQGDFHEACNFAGVNKLPVIIFC 187 G A ++ + + + ++ Sbjct: 148 AY-----FAQPTQGMQNAMGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVV 202 Query: 188 QNNQYAISVPAHKQLGGK-VSDRALGYGFPGVRVDGNDPLEVYRVVKEARERALAGEGPT 246 A L + G + + A+ + T Sbjct: 203 LILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLT 262 Query: 247 LIEAMMYRLSPHSTS-DNDLAYRTKEEVDANWA-KDGIAAFRSYLTELGLWSDEQESDLA 304 + S + ++A ++ + + + E + +Q +D A Sbjct: 263 DTVTHVREGSDAIYAGTREIAAGNT-DLSSRTEQQASALEETAASMEQLTATVKQNADNA 321 Query: 305 AEYNLELKAAIEYAENAPFPKPEDTLLHVY 334 + + ++A + A++ Sbjct: 322 RQASQLAQSASDTAQHGGKVVDGVVKTMHE 351 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.134 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 25,866,886 Number of extensions: 1074943 Number of successful extensions: 1707 Number of sequences better than 1.0e-05: 43 Number of HSP's gapped: 1629 Number of HSP's successfully gapped: 43 Length of query: 343 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 236 Effective length of database: 26,037,693 Effective search space: 6144895548 Effective search space used: 6144895548 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 116 (49.3 bits)