BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0386.0003.p (224 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin1408 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase comple... 265 6e-71 BS_yqiV [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase comple... 264 1e-70 BH2764 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex... 263 2e-70 SA1349 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex... 186 2e-47 BS_acoL [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase comple... 179 3e-45 >lin1408 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes Length = 553 Score = 265 bits (676), Expect = 6e-71, Method: Compositional matrix adjust. Identities = 11/221 (4%), Positives = 37/221 (16%), Gaps = 2/221 (0%) Query: 3 TESEVTIEAR-KGEQTQSLSAEKLLVSVGRVANIENIGLENTDIHFGKGVIEVNDNMQTN 61 + S + A ++ + T Sbjct: 254 VSHMQRSLTDTVTHV-REGSDAIYAGTREIAAGNTDLSSRTEQQASALEETAASMEQLTA 312 Query: 62 EPHIYAIGDCIGGLQLAHAASHEGIRAVDHLAGETLHPYHPHLVPRCVYTRPEVASVGYT 121 A A+ + + + + Sbjct: 313 TVKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQ 372 Query: 122 EKEARALGADVVTGKFPFSAIGKAIVYGMKDGFVKVVADRSSGDILGVQMIGPHVTDLIG 181 A + +++ + L + T + Sbjct: 373 TNILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVL 432 Query: 182 EAALAQLLDATPWEIGEAVHAHPTLSEIIGEAMLAVDGRAI 222 + + ++ + ++ E +D A+ Sbjct: 433 VESAGETMNNIVNAVTRVTDIMGEIASASDEQSRGIDQVAL 473 >BS_yqiV [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes Length = 553 Score = 264 bits (674), Expect = 1e-70, Method: Compositional matrix adjust. Identities = 23/198 (11%), Positives = 42/198 (21%) Query: 3 TESEVTIEARKGEQTQSLSAEKLLVSVGRVANIENIGLENTDIHFGKGVIEVNDNMQTNE 62 + S + ++ + +A T+ Q Sbjct: 253 SVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQASALEETAASMEQLTA 312 Query: 63 PHIYAIGDCIGGLQLAHAASHEGIRAVDHLAGETLHPYHPHLVPRCVYTRPEVASVGYTE 122 + QLA +AS + G + + + V + Sbjct: 313 TVKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQ 372 Query: 123 KEARALGADVVTGKFPFSAIGKAIVYGMKDGFVKVVADRSSGDILGVQMIGPHVTDLIGE 182 AL A V + G A+V G A + ++ V Sbjct: 373 TNILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVL 432 Query: 183 AALAQLLDATPWEIGEAV 200 A V Sbjct: 433 VESAGETMNNIVNAVTRV 450 >BH2764 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes Length = 553 Score = 263 bits (672), Expect = 2e-70, Method: Compositional matrix adjust. Identities = 23/221 (10%), Positives = 43/221 (19%) Query: 4 ESEVTIEARKGEQTQSLSAEKLLVSVGRVANIENIGLENTDIHFGKGVIEVNDNMQTNEP 63 S + ++ + +A T+ Q Sbjct: 254 VSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQASALEETAASMEQLTAT 313 Query: 64 HIYAIGDCIGGLQLAHAASHEGIRAVDHLAGETLHPYHPHLVPRCVYTRPEVASVGYTEK 123 + QLA +AS + G + + + V + Sbjct: 314 VKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQT 373 Query: 124 EARALGADVVTGKFPFSAIGKAIVYGMKDGFVKVVADRSSGDILGVQMIGPHVTDLIGEA 183 AL A V + G A+V G A + ++ V Sbjct: 374 NILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLV 433 Query: 184 ALAQLLDATPWEIGEAVHAHPTLSEIIGEAMLAVDGRAIGF 224 A V + + Sbjct: 434 ESAGETMNNIVNAVTRVTDIMGEIASASDEQSRGIDQVALA 474 >SA1349 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes Length = 553 Score = 186 bits (473), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 10/206 (4%), Positives = 35/206 (16%), Gaps = 3/206 (1%) Query: 20 LSAEKLLVSVGRVANIENIGLENTDIHFG-KGVIEVNDNMQTNEPHIYAIGDCIGGLQLA 78 + ++ G + + + + Q + Sbjct: 266 THVREGSDAIYAGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQAS 325 Query: 79 HAASHEGIRAVDHLAGETLHPYHPHLVPRCVYTRPEVASVGYTEKEARALGADVVTGKFP 138 A A H + ++ SV + A + Sbjct: 326 QLAQSASDTAQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAA 385 Query: 139 FSAIGKAIV--YGMKDGFVKVVADRSSGDILGVQMIGPHVTDLIGEAALAQLLDATPWEI 196 + + + + +++ +I + D + Sbjct: 386 RAGEQGRGFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVN 445 Query: 197 GEAVHAHPTLSEIIGEAMLAVDGRAI 222 + + Sbjct: 446 AVTRVTDIMGEIASASDEQSRGIDQV 471 >BS_acoL [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes Length = 553 Score = 179 bits (455), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 13/211 (6%), Positives = 42/211 (19%), Gaps = 1/211 (0%) Query: 12 RKGEQTQSLSAEKLLVSVGRVANIENIGLENTDIHFGKGVIEVNDNMQTNEPHIYAIGDC 71 ++ L + + G +++ + + Sbjct: 247 MGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQASALEETAAS 306 Query: 72 IGGLQLAHAASHEGIRAVDHLAGETLHPYHPHLVPRCVYTRPEVASVGYTEKEARALGAD 131 + L + + R LA + + + K+ + + Sbjct: 307 MEQLTATVKQNADNARQASQLAQSASDTAQHG-GKVVDGVVKTMHEIADSSKKIADIISV 365 Query: 132 VVTGKFPFSAIGKAIVYGMKDGFVKVVADRSSGDILGVQMIGPHVTDLIGEAALAQLLDA 191 + F + + + + +A + + Sbjct: 366 IDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQAAKEIKALIEDSVSR 425 Query: 192 TPWEIGEAVHAHPTLSEIIGEAMLAVDGRAI 222 A T++ I+ D Sbjct: 426 VDTGSVLVESAGETMNNIVNAVTRVTDIMGE 456 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.136 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,249,916 Number of extensions: 751246 Number of successful extensions: 3505 Number of sequences better than 1.0e-05: 157 Number of HSP's gapped: 3284 Number of HSP's successfully gapped: 157 Length of query: 224 Length of database: 38,378,431 Length adjustment: 103 Effective length of query: 121 Effective length of database: 26,499,029 Effective search space: 3206382509 Effective search space used: 3206382509 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 114 (48.5 bits)