BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0386.0002.p (221 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yqiV [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase comple... 236 3e-62 BH2764 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex... 225 4e-59 lin1408 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase comple... 201 1e-51 SA1349 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex... 194 8e-50 RC1239 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex... 160 2e-39 >BS_yqiV [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes Length = 553 Score = 236 bits (601), Expect = 3e-62, Method: Compositional matrix adjust. Identities = 15/218 (6%), Positives = 41/218 (18%), Gaps = 1/218 (0%) Query: 1 MTISCDVAILGGGTGGYVAAIRAAQLGKSVVVIEMDKLGGTCLHRGCIPSKSLLRSAEVY 60 M V L G A ++ + + ++ L + ++ Sbjct: 1 MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLM 60 Query: 61 AEIQESESYGIETAGVQLVFPKVQARKEAVVEQLHQGVQFLMRKNKIQVLKGKGRIIGPS 120 + + + S + + A+ E + Q K + + ++ S Sbjct: 61 LQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATS 120 Query: 121 IFSPRSGAVAVELENG-EMETVVSGNLIVATGSRPRVLPGLKPDGKVIXXXXXXXXXXXX 179 + + Sbjct: 121 RNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIV 180 Query: 180 XXXXXXXXXXXXXXEWASMLADFGVQVTVVEAAGQLLP 217 +++ + V LL Sbjct: 181 TDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT 218 >BH2764 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes Length = 553 Score = 225 bits (573), Expect = 4e-59, Method: Compositional matrix adjust. Identities = 15/222 (6%), Positives = 43/222 (19%), Gaps = 1/222 (0%) Query: 1 MTISCDVAILGGGTGGYVAAIRAAQLGKSVVVIEMDKLGGTCLHRGCIPSKSLLRSAEVY 60 M V L G A ++ + + ++ L + ++ Sbjct: 1 MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLM 60 Query: 61 AEIQESESYGIETAGVQLVFPKVQARKEAVVEQLHQGVQFLMRKNKIQVLKGKGRIIGPS 120 + + + S + + A+ E + Q K + + ++ S Sbjct: 61 LQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATS 120 Query: 121 IFSPRSGAVAVELENGEME-TVVSGNLIVATGSRPRVLPGLKPDGKVIXXXXXXXXXXXX 179 ++ + + Sbjct: 121 RNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIV 180 Query: 180 XXXXXXXXXXXXXXEWASMLADFGVQVTVVEAAGQLLPXXXA 221 +++ + V LL Sbjct: 181 TDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAK 222 >lin1408 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes Length = 553 Score = 201 bits (510), Expect = 1e-51, Method: Compositional matrix adjust. Identities = 13/221 (5%), Positives = 39/221 (17%), Gaps = 6/221 (2%) Query: 1 MTISCDVAILGGGTGGYVAAIRAAQLGKSVVVIEMDKLGGTCLHRGCIPSKSLLRSAEVY 60 M V L G A ++ + + ++ L + ++ Sbjct: 1 MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLM 60 Query: 61 AEIQESESYGIE---TAGVQLVFPKVQARKEAVVEQLHQGVQFLMRKNKIQVLKGKGRII 117 + + + S + + K + + Sbjct: 61 LQTRINLSRSAVRMMMDSSN--QQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVA 118 Query: 118 GPSIFSPRSGAVAVELENG-EMETVVSGNLIVATGSRPRVLPGLKPDGKVIXXXXXXXXX 176 + L + + A ++ + + Sbjct: 119 TSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRD 178 Query: 177 XXXXXXXXXXXXXXXXXEWASMLADFGVQVTVVEAAGQLLP 217 A ++ + L P Sbjct: 179 IVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTP 219 >SA1349 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes Length = 553 Score = 194 bits (493), Expect = 8e-50, Method: Compositional matrix adjust. Identities = 9/212 (4%), Positives = 27/212 (12%) Query: 6 DVAILGGGTGGYVAAIRAAQLGKSVVVIEMDKLGGTCLHRGCIPSKSLLRSAEVYAEIQE 65 V +L G + + + K + + Sbjct: 7 VVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLMLQTRIN 66 Query: 66 SESYGIETAGVQLVFPKVQARKEAVVEQLHQGVQFLMRKNKIQVLKGKGRIIGPSIFSPR 125 + + + K + + + Sbjct: 67 LSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATSRNIDEK 126 Query: 126 SGAVAVELENGEMETVVSGNLIVATGSRPRVLPGLKPDGKVIXXXXXXXXXXXXXXXXXX 185 L + + Sbjct: 127 YKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIVTDNADD 186 Query: 186 XXXXXXXXEWASMLADFGVQVTVVEAAGQLLP 217 +++ + V LL Sbjct: 187 YRFAQWQLAVIALVVVLILLVAWYGIRRMLLT 218 >RC1239 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes Length = 553 Score = 160 bits (404), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 13/212 (6%), Positives = 31/212 (14%), Gaps = 11/212 (5%) Query: 6 DVAILGGGTGGYVAAIRAAQLGKSVVVIEMDKLGGTCLHRGCIPSKSLLRSAEVYAEIQE 65 + V A+ G + + L S Sbjct: 5 IRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLMLQTR 64 Query: 66 SESYGIETAGVQLVFPKVQARKEAVVEQLHQGVQFLMRKNKIQVLKGKGRIIGPSIFSPR 125 + + K +++ + + K + + + Sbjct: 65 INLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATSRNID 124 Query: 126 SGAVAVELENGEMETVVSGNLIVATGSRPRVLPGLKPDGKVIXXXXXXXXXXXXXXXXXX 185 L G Sbjct: 125 EKYK-----------NYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSE 173 Query: 186 XXXXXXXXEWASMLADFGVQVTVVEAAGQLLP 217 + A Q+ V+ L+ Sbjct: 174 KLYRDIVTDNADDYRFAQWQLAVIALVVVLIL 205 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.135 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 13,485,182 Number of extensions: 492397 Number of successful extensions: 2335 Number of sequences better than 1.0e-05: 140 Number of HSP's gapped: 2098 Number of HSP's successfully gapped: 140 Length of query: 221 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 119 Effective length of database: 26,614,363 Effective search space: 3167109197 Effective search space used: 3167109197 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 114 (48.5 bits)