BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0385.0015.p (465 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PAB0119 [G] COG2182 Maltose-binding periplasmic proteins/domains 92 2e-18 BMEII0542 [G] COG1653 ABC-type sugar transport system, periplasm... 81 4e-15 all1916 [G] COG1653 ABC-type sugar transport system, periplasmic... 80 5e-15 PH0753 [G] COG1653 ABC-type sugar transport system, periplasmic ... 79 1e-14 mll0854 [G] COG1653 ABC-type sugar transport system, periplasmic... 77 7e-14 >PAB0119 [G] COG2182 Maltose-binding periplasmic proteins/domains Length = 453 Score = 92.0 bits (227), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 97/349 (27%), Positives = 160/349 (45%), Gaps = 35/349 (10%) Query: 38 KTSDAGAASGEKTKITYWTGDRHDAEFVKEKVAEFNKTNTDGIEVELVVKGDDFDTALDL 97 T +A SG K+ W + + V + +AE +E+ K D + AL + Sbjct: 69 HTQEAECGSG---KVVIWHNMQPNELQVFQSLAEEYMAMCPDVEIVFEQK-PDLENALKV 124 Query: 98 SFQTSDSPDVIRVKENTIGTFYKKEYLAPIDEYLSDDMKAKFPEMTDLNI-FDGKRYSLP 156 + PD+ + IG F + L PIDEY++DD+ KF M I + G Y+LP Sbjct: 125 AIPAGQGPDLFIWAHDWIGKFAEAGLLEPIDEYITDDLLQKFAPMAREAIEYKGHYYALP 184 Query: 157 NYGTTMRLIYNKDLFAKAGVTNPPTTLQELVDTAKKLTAAGKADGAYGFAQNFKSPASAF 216 T+ +IYNK + V+ PP T EL + +K + YG A P +A+ Sbjct: 185 FAAETVAMIYNKKI-----VSEPPKTFDELKEVMEKYYDPN--NEKYGIAW----PINAY 233 Query: 217 GRSARVIAEMSGFGGFGYDFKTARFDFSGFKP-IIEAFKQIRDD--GSMLPGVESLDIDP 273 SA IA+ FGG+ +D KT + KP IE FK ++ M P + D + Sbjct: 234 FISA--IAQ--AFGGYYFDDKTEQPGLD--KPETIEGFKFFFENIWPYMAP---TADYNT 284 Query: 274 LRAQFAEGKIGMYLSYSSEPGVYSTQFPAKIDWAAAPAPTI--DGKVKGASGFLGGQWLA 331 ++ F EG+ + + + P + A ID+ AP P I DGK + G + + Sbjct: 285 QQSIFLEGRAPIMV---NGPWSIGSVKDAGIDFGVAPLPPIIKDGKEYWPRPYGGVKLIY 341 Query: 332 LSSKSKHKDAAWKFMEYMYSDQMLTTYQEKGFGISMVPSISAAAKTPEV 380 ++ + +KDAAWKF+++ ++ + ++ + +P +S PE+ Sbjct: 342 FAAGTHNKDAAWKFVKWFTTNPEVI--KQLALDLGYIPVLSEVLNDPEI 388 >BMEII0542 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 398 Score = 80.9 bits (198), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 84/309 (27%), Positives = 130/309 (42%), Gaps = 29/309 (9%) Query: 67 EKVAEFNKTNTDGIEVEL-VVKGDDFDTALDLSFQTSDSPDVIRVKENTIGTFYKKEYLA 125 E+V + + GI + VV D L + D+ + + F ++ Sbjct: 31 EEVKKAFEAENPGITLNFEVVPWDVLLQKLTTDISAGTNADLSIIGTRWLIDFVQQGIAE 90 Query: 126 PIDEYLSDDMKAKFPE-MTDLNIFDGKRYSLPNYGTTMRLIYNKDLFAKAGVTNPPTTLQ 184 P+D Y++D+ K +F E ++ DGK Y LP + + YNKDLF KAG+ NPP Sbjct: 91 PLDGYMNDEFKGRFIETFLSPSVLDGKTYGLPIAASVRAMYYNKDLFEKAGIKNPPANWD 150 Query: 185 ELVDTAKKLTAAGKADGAYGFAQNFKSPAS------AFGRSARVIAEMSGFGGFGYDFKT 238 EL A K+ A G + YGF K + A I G T Sbjct: 151 ELKADAAKIKALGGEN--YGFGLQGKEIETDVYYYYAMWSYGTEILNKDG---------T 199 Query: 239 ARFDFSGFKPIIEAFKQIRDDGSMLPGVESLDIDPLRAQFAEGKIGMYLSYSSEPGVYST 298 + G + +K + DDG PGV S + ++ F +GK+GM ++ Sbjct: 200 SGLSTPGALEAAKLYKSMIDDGLTQPGVTSYAREDVQNLFKQGKVGMMITAPFLSNQIKE 259 Query: 299 QFP-AKIDWAAAPA-PTIDGKVKGASGFLG-GQWLALSSKSKHKDAAWKFMEYMYSDQML 355 + P K AA PA PT GA G G + + SK+K+ AWK +++++ Sbjct: 260 EAPNLKYGVAAIPAGPT------GARGTYGVTDSVIMFQNSKNKEEAWKVLDFLFQKDWR 313 Query: 356 TTY-QEKGF 363 + Q +GF Sbjct: 314 AKFTQNEGF 322 >all1916 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 418 Score = 80.5 bits (197), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 90/387 (23%), Positives = 164/387 (42%), Gaps = 50/387 (12%) Query: 50 TKITYWTGDRHDAEFVKEKVAEFNKTNTDGIEVELVVKGDDFDTALDLSFQTSDSPDVIR 109 K++ W G + + +K+ + +F + I+V+ V D + + +PDV Sbjct: 39 VKLSGWGGSPVEQKLLKQVLQDFEAQHPH-IKVKYEVISDQYMDVIKTRLIGEAAPDVFY 97 Query: 110 VKENTIGTFYKKEYLAPIDEYLSDDMKAKFPEMTDL--NIFDGKRYSLPNYG-----TTM 162 + + L P+D Y++ + +++D N+ D +Y YG +T+ Sbjct: 98 LDALEAPFLMSQNVLEPLDNYITPEF-----DLSDFEANLLDNFKYQNHIYGFPKDYSTL 152 Query: 163 RLIYNKDLFAKAGVTNPPTTLQELVDTAKKLTAAGKADGAYGFAQNFKSPASAFGRSARV 222 L YNK FA AG+++PP T EL +++LT GK + YGF + +P AR Sbjct: 153 ALFYNKKAFAAAGLSSPPATWTELRTYSQQLT--GKLN-KYGFGE---AP-----ELARQ 201 Query: 223 IAEMSGFGG-----FGYDFKTARFDFSGFKPIIEAFKQIRDDGSMLPGVESLDIDPLRAQ 277 + ++ FGG GY + GF+ +I+ +++ + + + Sbjct: 202 VYKIKAFGGEVINQNGYATFASEAGLRGFQLVIDQYQKDKSSAQK----SDVGTNSGSEM 257 Query: 278 FAEGKIGMYLSYSSEPGVYSTQFPAKIDWAAAPAPTIDGKVKGASGFLGGQWLALSSKSK 337 F + K+ M + + FP ++++A A PTI+ K KG F +S +S+ Sbjct: 258 FGQEKVAMVIEGNWAIPYLQETFP-QLEFATAQLPTINNK-KGTMVFTVA--YVMSKQSQ 313 Query: 338 HKDAAWKFMEYMYSDQMLTTYQEKGFGISMVPSISAA-------AKTPEVNGIEGFLPNQ 390 HK AW+ + Y+ + + GF + S+S ++P V G++ P Sbjct: 314 HKAEAWELISYLTGKAGMQKWTSTGFALPTRKSVSQKFGYEQDPLRSPLVAGVDDATP-- 371 Query: 391 YDGVWPVYPSVAPEGMKSDDAFFKYML 417 W V AP D+ F +L Sbjct: 372 ----WQVGKYPAPIVNNFDNQFVSALL 394 >PH0753 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 464 Score = 79.3 bits (194), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 96/376 (25%), Positives = 152/376 (40%), Gaps = 33/376 (8%) Query: 1 MKKQLLL--TSLSAVLAFGLAACGNSNTAENSGEASNAGKTSDAGAASGEK--------- 49 M+K LL+ L+ VL+ A C T + S S G S + E Sbjct: 4 MRKPLLVGFLILALVLSTIAAGCIGGGTTQTSPTQSPTGTQSPTPTQTTETITKTETKTM 63 Query: 50 ------TKITYWTGDRHDAEFVKEKVAEFNKTNTDGIEVELVVKGDDFDTALDLSFQTSD 103 + W+ + + + + F K N + I+V+ V F + SF Sbjct: 64 IQNQVFIRFAGWSAGETEMKNYQRMIELFEKENPN-IKVKYEVITQMFHENILASFGAGV 122 Query: 104 SPDVIRVKENTIGTFYKKEYLAPIDEYLSDDMKAKF-PEMTDLNIFDGKRYSLPNYGTTM 162 +PDV V + F +K L PI E + KF P + + +GK Y LP + + Sbjct: 123 APDVFYVDSSWAPIFIEKGALYPISELADESFINKFYPFLLEPFKKNGKLYGLPKDWSML 182 Query: 163 RLIYNKDLFAKAGVTNPPTTLQELVDTAKKLTAAGKADGAYGFAQNFKS--PASAFGRSA 220 L YNK LF +AG+T PP T EL++ AK +T G + F P + + Sbjct: 183 ALFYNKKLFEQAGLTKPPETWDELLEYAKIITEKTGKPGLAIYLGGFNRYVPVAVSNGAP 242 Query: 221 RVIAEMSGFGGFGYDFKTARFDFSGFKPIIEAFKQIRDDGSMLPGV---ESLDIDPLRAQ 277 + E + +D R + + + K R+ + P V E + L Sbjct: 243 KPWFEKPEDASW-FDNPVVRETLTWYINLYRKGKVEREKKGLPPYVVAPEDVGAGWLGDA 301 Query: 278 FAEGKIGMYLSYSSEPGVYSTQFPA---KIDWAAAPAPT-IDGKVKGASGFLGGQWLALS 333 F + K+ M +S + + QFP DW AP P +G+V + L ++ Sbjct: 302 FGQQKVAMVISGNWMIPFLADQFPNFKYGEDWDIAPLPAGKNGRVTMVYTVI----LGIN 357 Query: 334 SKSKHKDAAWKFMEYM 349 SK+KH AWKF+E++ Sbjct: 358 SKTKHPKEAWKFVEFI 373 >mll0854 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 430 Score = 77.0 bits (188), Expect = 7e-14, Method: Compositional matrix adjust. Identities = 90/327 (27%), Positives = 143/327 (43%), Gaps = 20/327 (6%) Query: 33 ASNAGKTSDAGAASGEKT-KITYWTGDRHDAEFVKEKVAEFNKTNTDGIEVELVVK--GD 89 A+ AG AG+A + T K+ EF+K+++AEF N G++VEL+ G Sbjct: 14 ATVAGLAISAGSALADSTLKMVEVITSPPRTEFLKKQIAEFEAANP-GVKVELISLPWGQ 72 Query: 90 DFDTALDLSFQTSDSPDVIRVKENTIGTFYKKEYLAPIDEYLS--DDMKAKFPEMTDL-N 146 F+ L + Q D+PDV+ + E +G + L + Y++ DD K + Sbjct: 73 AFEKFLTM-VQAGDTPDVVEMPERWMGLYANNAQLEDLGPYMAKWDDAKTLGERAKQFGS 131 Query: 147 IFDGKRYSLPNYGTTMR-LIYNKDLFAKAGVTNPPTTLQELVDTAKKLTAAGKADGAYGF 205 + ++ +P YG + L +NK LF +AG+ PP TL E V +KK++A G YG+ Sbjct: 132 TVNNTQFMIP-YGYYVNALFWNKKLFKQAGLDGPPATLDEFVADSKKISA---IPGKYGY 187 Query: 206 AQNFKSPASAFGRSARVIAEMSGFGGFGYDFKTARFDFSGFKPIIEAFKQIRDDGSMLPG 265 + AF + G GG+ T+ + G ++ + DG Sbjct: 188 C--LRGGPGAFNGMHMFMNIAQGKGGYFNKDGTSTINEEGSVKGLQMLADMYKDGLAPKD 245 Query: 266 VESLDIDPLRAQFAEGKIGMYLSYSSEPGVYSTQFPAKIDWAAAPAPT-IDGKVKGASGF 324 S + F G M L+ + + + D+A AP P GK G+ Sbjct: 246 AVSWGFNETVTGFYSGTCAM-LNQDPDALLGIADKMSADDFAVAPLPVGPSGKSYPTLGY 304 Query: 325 LGGQWLALSSKSKHKDAAWKFMEYMYS 351 G W A+ + S+HKD AWK M + S Sbjct: 305 AG--W-AMFANSQHKDDAWKLMATLLS 328 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.133 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 36,300,202 Number of extensions: 1670128 Number of successful extensions: 2459 Number of sequences better than 1.0e-05: 46 Number of HSP's gapped: 2454 Number of HSP's successfully gapped: 46 Length of query: 465 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 355 Effective length of database: 25,691,691 Effective search space: 9120550305 Effective search space used: 9120550305 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 118 (50.1 bits)