BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0385.0014.p (300 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin0218 [G] COG1175 ABC-type sugar transport systems, permease c... 155 6e-38 Rv2040c [G] COG1175 ABC-type sugar transport systems, permease c... 150 2e-36 MT2100 [G] COG1175 ABC-type sugar transport systems, permease co... 150 2e-36 SMb20232 [G] COG1175 ABC-type sugar transport systems, permease ... 149 7e-36 slr1202 [G] COG1175 ABC-type sugar transport systems, permease c... 147 2e-35 >lin0218 [G] COG1175 ABC-type sugar transport systems, permease components Length = 292 Score = 155 bits (393), Expect = 6e-38, Method: Compositional matrix adjust. Identities = 91/277 (32%), Positives = 149/277 (53%), Gaps = 12/277 (4%) Query: 1 MNKSKNAAFAYSFLLPSALLTLVLGIYPIAWAFRYMFYNYKGYGTARFIGLDNFSRILRD 60 M + K + FLLP+ L L+ PI + F ++ T F G +N+ R+ D Sbjct: 4 MTRKKTLFWLCIFLLPNLLGFLIFIAIPILGSLGISFTDWDLVSTIHFTGFENYKRLFND 63 Query: 61 TEFWNSVVNTFVYAGGKXXXXXXXXXXXXXXXNRKIRGRQLLRAVFFMPTVISTAVMAVV 120 EFW S NT + G N+KI+ R RAV+F+P + S +++V Sbjct: 64 PEFWKSFRNTITFIIGYLPSVMILGLACALMLNQKIKLRPFFRAVYFLPVITSWVAVSLV 123 Query: 121 FFTIFNSYNGILNQFLIKFNISSSGIDWL-GPKHAMLTVILVAVWGAVGNYMLLFLAGLQ 179 + +FN G++N FL I+ G +WL P+ AM+ VI+ + W +G M+LFL GLQ Sbjct: 124 WKWLFNPSYGLINYFLSLIGIA--GPNWLTDPQTAMIAVIITSAWKDIGFVMVLFLGGLQ 181 Query: 180 NIPEDVYESSSLDGANKIQQFRFITLPMLGPVLQMVIMLAIINALKGYESIMVLTEGGPA 239 NI YE++++DGA+K++Q IT+P+L P V ++++IN+ + ++ +M++TEGGP+ Sbjct: 182 NISPSYYEAANMDGASKLRQLFSITIPLLTPTTFFVTIISLINSFQVFDQVMIMTEGGPS 241 Query: 240 GKTEVMFLYLYKLFFPVGGSAASTQVQEFGYGSAVAF 276 G T + +Y F + E GY SA+++ Sbjct: 242 GATMTLVQNIYNHAF---------RYFEMGYASAMSW 269 >Rv2040c [G] COG1175 ABC-type sugar transport systems, permease components Length = 300 Score = 150 bits (379), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 98/287 (34%), Positives = 158/287 (55%), Gaps = 15/287 (5%) Query: 13 FLLPSALLTLVLGIYPIAWAFRYMFYNYKGYGTARFIGLDNFSRIL-RDTEFWNSVVNTF 71 F+ P+ +V ++P+ ++ F + + A F+ LDNF + D F +VVNT Sbjct: 15 FVAPNLAAVVVFMLFPLGFSLYMSFQKWDLFTHATFVRLDNFRNLFTSDPLFLIAVVNTA 74 Query: 72 VYAGGKXXXXXXXXXXXXXXXNRKIRGRQLLRAVFFMPTVISTAVMAVVFFTIFNSYNGI 131 VY G NRKI+G L R V F+P IS+ VMAVV+ +FN+ NG+ Sbjct: 75 VYTVGTVVPTVIVSLVVAAFLNRKIKGISLFRTVVFLPLAISSVVMAVVWQFVFNTDNGL 134 Query: 132 LNQFLIKFNISSSGIDWL-GPKHAMLTVILVAVWGAVGNYMLLFLAGLQNIPEDVYESSS 190 LN L I I WL P+ AM+++ LV+VW +V ++ LA +Q +PE VYE++ Sbjct: 135 LNIMLGWLGIGP--IPWLIEPRWAMVSLCLVSVWRSVPFATVVLLAAMQGVPETVYEAAR 192 Query: 191 LDGANKIQQFRFITLPMLGPVLQMVIMLAIINALKGYESIMVLT--EGGPAGKTEVMFLY 248 +DGA +I+QF IT+P++ L V++++II+A + ++ + VLT GGP T V+ + Sbjct: 193 IDGAGEIRQFVSITVPLIRGALSFVVVISIIHAFQAFDLVYVLTGANGGPETATYVLGIM 252 Query: 249 LYKLFFPVGGSAASTQVQEFGYGSAVAFVSAVIVGMISLIYFYASRR 295 L++ F EFGY SA+A+V I+ +++++ + R Sbjct: 253 LFQHAF---------SFLEFGYASALAWVMFAILLVLTVLQLRITHR 290 >MT2100 [G] COG1175 ABC-type sugar transport systems, permease components Length = 300 Score = 150 bits (379), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 98/287 (34%), Positives = 158/287 (55%), Gaps = 15/287 (5%) Query: 13 FLLPSALLTLVLGIYPIAWAFRYMFYNYKGYGTARFIGLDNFSRIL-RDTEFWNSVVNTF 71 F+ P+ +V ++P+ ++ F + + A F+ LDNF + D F +VVNT Sbjct: 15 FVAPNLAAVVVFMLFPLGFSLYMSFQKWDLFTHATFVRLDNFRNLFTSDPLFLIAVVNTA 74 Query: 72 VYAGGKXXXXXXXXXXXXXXXNRKIRGRQLLRAVFFMPTVISTAVMAVVFFTIFNSYNGI 131 VY G NRKI+G L R V F+P IS+ VMAVV+ +FN+ NG+ Sbjct: 75 VYTVGTVVPTVIVSLVVAAFLNRKIKGISLFRTVVFLPLAISSVVMAVVWQFVFNTDNGL 134 Query: 132 LNQFLIKFNISSSGIDWL-GPKHAMLTVILVAVWGAVGNYMLLFLAGLQNIPEDVYESSS 190 LN L I I WL P+ AM+++ LV+VW +V ++ LA +Q +PE VYE++ Sbjct: 135 LNIMLGWLGIGP--IPWLIEPRWAMVSLCLVSVWRSVPFATVVLLAAMQGVPETVYEAAR 192 Query: 191 LDGANKIQQFRFITLPMLGPVLQMVIMLAIINALKGYESIMVLT--EGGPAGKTEVMFLY 248 +DGA +I+QF IT+P++ L V++++II+A + ++ + VLT GGP T V+ + Sbjct: 193 IDGAGEIRQFVSITVPLIRGALSFVVVISIIHAFQAFDLVYVLTGANGGPETATYVLGIM 252 Query: 249 LYKLFFPVGGSAASTQVQEFGYGSAVAFVSAVIVGMISLIYFYASRR 295 L++ F EFGY SA+A+V I+ +++++ + R Sbjct: 253 LFQHAF---------SFLEFGYASALAWVMFAILLVLTVLQLRITHR 290 >SMb20232 [G] COG1175 ABC-type sugar transport systems, permease components Length = 314 Score = 149 bits (375), Expect = 7e-36, Method: Compositional matrix adjust. Identities = 98/297 (32%), Positives = 161/297 (54%), Gaps = 17/297 (5%) Query: 3 KSKNAAFAYSFLLPSAL-LTLVLGIYPIAWAFRYMFYNYKGYGTARFIGLDNFSRILRDT 61 K + Y F+LP AL L + LG+ P+A + + G+G F+G N+ R+ D Sbjct: 22 KHREWIAGYLFVLPDALGLFIFLGV-PMALSLVLSVFEVNGFGGYSFVGARNYLRMWNDP 80 Query: 62 EFWNSVVNTFVYAGGKXXXXXXXXXXXXXXXNRKIRGRQLLRAVFFMPTVISTAVMAVVF 121 FW T +YA R R ++R++FF P ++S V+A+V+ Sbjct: 81 LFWKGARVTALYAAMLVPLLYVCGLGLALLVQRTDRFNAIMRSMFFAPHMVSLVVVALVW 140 Query: 122 FTIFNSYNGILNQFLIKFNISSSGIDWLG-PKHAMLTVILVAVWGAVGNYMLLFLAGLQN 180 + G++++ +I GI LG P A++T++LV+VW +G YML+FL GLQ+ Sbjct: 141 QFMVVDKIGVVSRLTAALDIG--GISLLGDPNFALITIVLVSVWFLMGFYMLIFLGGLQD 198 Query: 181 IPEDVYESSSLDGANKIQQFRFITLPMLGPVLQMVIMLAIINALKG---YESIMVLTEGG 237 IP YE++ +DGA I +F FITLP+L P VIM++++ A+ G ++ I V+T+GG Sbjct: 199 IPRQYYEAAMIDGAGAIARFWFITLPLLKPTSFFVIMVSMVAAVAGAQAFDIIYVMTKGG 258 Query: 238 PAGKTEVMFLYLYKLFFPVGGSAASTQVQEFGYGSAVAFVSAVIVGMISLIYFYASR 294 PA T V+ +Y+Y+ F G FGY +A+A + V + +++ ++F +R Sbjct: 259 PANATSVLIVYIYQQAFGFG---------AFGYAAAMASILVVALMIVTAVFFALTR 306 >slr1202 [G] COG1175 ABC-type sugar transport systems, permease components Length = 298 Score = 147 bits (372), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 94/290 (32%), Positives = 153/290 (52%), Gaps = 14/290 (4%) Query: 2 NKSKNAAFAYSFLLPSALLTLVLGIYPIAWAFRYMFYNYKGYGT--ARFIGLDNFSRILR 59 + A Y FLLP+ ++ YP AF F Y+ T ++GL NF R+ R Sbjct: 8 RRWPRGATPYLFLLPALVMLGATVFYPALQAFSLSFTQYELDLTKAPAWVGLANFERLWR 67 Query: 60 DTEFWNSVVNTFVYAGGKXXXXXXXXXXXXXXXNRKIRGRQLLRAVFFMPTVISTAVMAV 119 D FW + +T +Y G N+K+RG R ++ P + S V + Sbjct: 68 DRVFWLTFRHTLLYLVGVVPLLIMLPLGLAILVNQKLRGITWFRMAYYTPVITSIVVAGI 127 Query: 120 VFFTIFNSYNGILNQFLIKFNISSSGIDWL-GPKHAMLTVILVAVWGAVGNYMLLFLAGL 178 + ++ S NGILNQ L S GI WL P A+ +V++V VW +G YM+++LAGL Sbjct: 128 AWKALYAS-NGILNQILALLGFSD-GIPWLTSPNLALWSVMVVTVWKGLGYYMVIYLAGL 185 Query: 179 QNIPEDVYESSSLDGANKIQQFRFITLPMLGPVLQMVIMLAIINALKGYESIMVLTEGGP 238 Q IP+++YE+ ++DGA+ +Q IT+P++ P +V +L+ I+ALK +E + ++T+GGP Sbjct: 186 QAIPQELYEAGAIDGADGWRQHWDITIPLMRPYCFLVGVLSSISALKVFEEVYIMTQGGP 245 Query: 239 AGKTEVMFLYLYKLFFPVGGSAASTQVQEFGYGSAVAFVSAVIVGMISLI 288 ++ + Y+Y+ F Q E Y SA+ V +++ + S+I Sbjct: 246 LNASKTVVYYVYERAF---------QDLEMNYASAIGLVLFLVIFIFSVI 286 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.328 0.142 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,440,705 Number of extensions: 698789 Number of successful extensions: 2812 Number of sequences better than 1.0e-05: 156 Number of HSP's gapped: 2530 Number of HSP's successfully gapped: 157 Length of query: 300 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 194 Effective length of database: 26,153,027 Effective search space: 5073687238 Effective search space used: 5073687238 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 115 (48.9 bits)