BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0382.0031.p (418 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC3575 [I] COG0331 (acyl-carrier-protein) S-malonyltransferase 254 1e-67 BS_fabD [I] COG0331 (acyl-carrier-protein) S-malonyltransferase 211 1e-54 YPO1598 [I] COG0331 (acyl-carrier-protein) S-malonyltransferase 191 1e-48 PA2968 [I] COG0331 (acyl-carrier-protein) S-malonyltransferase 187 4e-47 >CAC3575 [I] COG0331 (acyl-carrier-protein) S-malonyltransferase Length = 308 Score = 254 bits (650), Expect = 1e-67, Method: Compositional matrix adjust. Identities = 137/308 (44%), Positives = 189/308 (61%), Gaps = 3/308 (0%) Query: 1 MSKIAFLFSGQGSQYVGMGKSLYDNFSIAKETYAEASEVLGFDLGRLCFEGSSSELSRTE 60 M KIAF+FSGQGSQYVGMGK LYDN+ AKET+ +A EVLGF + LCFEG EL+ TE Sbjct: 1 MGKIAFVFSGQGSQYVGMGKDLYDNYQSAKETFDKADEVLGFKISELCFEGKDEELNLTE 60 Query: 61 NTQPAILAASIAQYRVYMQEIGEQPCYAAGHSLGEYSALAAAGAIEFADAVKLVRARGLY 120 NTQPA+L SIA R +E G +P AG SLGEYSA +G+ F DAV+LV+ RG Y Sbjct: 61 NTQPAVLITSIAALRALEEEKGIKPDVVAGLSLGEYSAHVCSGSFSFEDAVRLVKKRGRY 120 Query: 121 MKXXXXXXXXXXXXIRDVEDSVIEEECRTLSAQGRIIGISNYNGNKDIVVSGEAQAVERL 180 M+ I +E V+ C S +G I+ ++NYN IV++GE +AVE Sbjct: 121 MQEAVPKGIGTMAAIIGLEGDVVRGICAEQSKEG-IVEVANYNCPGQIVIAGEVKAVESA 179 Query: 181 VEVLKNQGA-QIFKLNVSAAFHSPIMEPAAVKLNRELGNYNFSQPQYQVISNVSALPYED 239 LK GA + L+VS FH+ +++ AA KL EL N N + VI+NV+ ED Sbjct: 180 CTKLKESGARRTVMLSVSGPFHTSMLKSAAEKLEEELKNINIEDMKVPVITNVTGDYVED 239 Query: 240 KGQIRDILTQQIVNPVNWYATMKYLERNGIDTAIEFGPKATLRNLVRN-NTGIAAYSYDK 298 K QI+ +L +Q+++ V W T++ + +G+DT IE GP TL + ++ N + ++ +K Sbjct: 240 KDQIKGLLKKQVMSSVRWEDTIRRMMDDGVDTFIELGPGKTLSSFIKKINRKMTIFNIEK 299 Query: 299 QEDIEEIQ 306 ED+ +IQ Sbjct: 300 AEDLNKIQ 307 >BS_fabD [I] COG0331 (acyl-carrier-protein) S-malonyltransferase Length = 317 Score = 211 bits (538), Expect = 1e-54, Method: Compositional matrix adjust. Identities = 91/287 (31%), Positives = 135/287 (47%), Gaps = 2/287 (0%) Query: 1 MSKIAFLFSGQGSQYVGMGKSLYDNFSIAKETYAEASEVLGFDLGRLCFEGSSSELSRTE 60 MSKIAFLF GQGSQ++GMGK LY+ AK + EA E L L L FEG + EL+ T Sbjct: 1 MSKIAFLFPGQGSQFIGMGKELYEQVPAAKRLFDEADETLETKLSSLIFEGDAEELTLTY 60 Query: 61 NTQPAILAASIAQYRVYMQEIGEQPCYAAGHSLGEYSALAAAGAIEFADAVKLVRARGLY 120 N QPA+L SIA + + A + +AA + + Sbjct: 61 NAQPALLTTSIAVLEKFKESGITPDFTAGHSLGEYSALVAAGALSFKDAVYTVRKRGEFM 120 Query: 121 MKXXXXXXXXXXXXIRDVEDSVIEEECRTLSAQGRIIGISNYNGNKDIVVSGEAQAVE-R 179 + + +++ + + + +G ++ ++N N IV+SG A+ VE Sbjct: 121 NEAVPAGEGAMAAILGMDAEALKQVTDKV-TEEGNLVQLANLNCPGQIVISGTAKGVELA 179 Query: 180 LVEVLKNQGAQIFKLNVSAAFHSPIMEPAAVKLNRELGNYNFSQPQYQVISNVSALPYED 239 +N + L VS FHS +M+PAA KL L + VISNVSA + Sbjct: 180 SELAKENGAKRAIPLEVSGPFHSELMKPAAEKLKEVLDACDIKDADVPVISNVSADVMTE 239 Query: 240 KGQIRDILTQQIVNPVNWYATMKYLERNGIDTAIEFGPKATLRNLVR 286 K I++ L +Q+ +PV + ++ L G+ T IE GP L LV+ Sbjct: 240 KADIKEKLIEQLYSPVRFEESINKLIAEGVTTFIEIGPGKVLSGLVK 286 Score = 206 bits (525), Expect = 4e-53, Method: Compositional matrix adjust. Identities = 119/287 (41%), Positives = 161/287 (56%), Gaps = 2/287 (0%) Query: 1 MSKIAFLFSGQGSQYVGMGKSLYDNFSIAKETYAEASEVLGFDLGRLCFEGSSSELSRTE 60 MSKIAFLF GQGSQ++GMGK LY+ AK + EA E L L L FEG + EL+ T Sbjct: 1 MSKIAFLFPGQGSQFIGMGKELYEQVPAAKRLFDEADETLETKLSSLIFEGDAEELTLTY 60 Query: 61 NTQPAILAASIAQYRVYMQEIGEQPCYAAGHSLGEYSALAAAGAIEFADAVKLVRARGLY 120 N QPA+L SIA + +E G P + AGHSLGEYSAL AAGA+ F DAV VR RG + Sbjct: 61 NAQPALLTTSIAVLEKF-KESGITPDFTAGHSLGEYSALVAAGALSFKDAVYTVRKRGEF 119 Query: 121 MKXXXXXXXXXXXXIRDVEDSVIEEECRTLSAQGRIIGISNYNGNKDIVVSGEAQAVERL 180 M I ++ +++ ++ +G ++ ++N N IV+SG A+ VE Sbjct: 120 MNEAVPAGEGAMAAILGMDAEALKQVTDKVTEEGNLVQLANLNCPGQIVISGTAKGVELA 179 Query: 181 VEVLKNQGAQ-IFKLNVSAAFHSPIMEPAAVKLNRELGNYNFSQPQYQVISNVSALPYED 239 E+ K GA+ L VS FHS +M+PAA KL L + VISNVSA + Sbjct: 180 SELAKENGAKRAIPLEVSGPFHSELMKPAAEKLKEVLDACDIKDADVPVISNVSADVMTE 239 Query: 240 KGQIRDILTQQIVNPVNWYATMKYLERNGIDTAIEFGPKATLRNLVR 286 K I++ L +Q+ +PV + ++ L G+ T IE GP L LV+ Sbjct: 240 KADIKEKLIEQLYSPVRFEESINKLIAEGVTTFIEIGPGKVLSGLVK 286 >YPO1598 [I] COG0331 (acyl-carrier-protein) S-malonyltransferase Length = 309 Score = 191 bits (486), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 105/287 (36%), Positives = 156/287 (54%), Gaps = 2/287 (0%) Query: 1 MSKIAFLFSGQGSQYVGMGKSLYDNFSIAKETYAEASEVLGFDLGRLCFEGSSSELSRTE 60 MSK A +F GQGSQ +GM L F I + T++EAS VLG+DL +L +G + EL++T Sbjct: 1 MSKFAMVFPGQGSQSLGMLADLAAQFPIVEATFSEASSVLGYDLWQLVQQGPAEELNKTW 60 Query: 61 NTQPAILAASIAQYRVYMQEIGEQPCYAAGHSLGEYSALAAAGAIEFADAVKLVRARGLY 120 TQPA+L AS+A +R++ + G+ P AGHSLGEYSAL AG ++F AV LV RG Sbjct: 61 QTQPALLTASVAIWRIWQHQGGKLPSLMAGHSLGEYSALVCAGVLDFKQAVSLVELRGKL 120 Query: 121 MKXXXXXXXXXXXXIRDVEDSVIEEECRTLSAQGRIIGISNYNGNKDIVVSGEAQAVE-R 179 M+ I +++ I + C SAQG+++ N+N +V++G AVE Sbjct: 121 MQEAVPEGTGAMYAIIGLDNESIAKACEE-SAQGQVVSPVNFNSPGQVVIAGNKDAVERA 179 Query: 180 LVEVLKNQGAQIFKLNVSAAFHSPIMEPAAVKLNRELGNYNFSQPQYQVISNVSALPYED 239 + L VS H +M+PAA KL L +F +P + V++NV Sbjct: 180 GAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVALEKIDFQEPLFPVVNNVDVKAEVS 239 Query: 240 KGQIRDILTQQIVNPVNWYATMKYLERNGIDTAIEFGPKATLRNLVR 286 IR L +Q+ NPV W +++++ G++ +E GP L L + Sbjct: 240 PVAIRSALVRQLYNPVRWTESVEFIAAEGVELLLEIGPGKVLTGLTK 286 >PA2968 [I] COG0331 (acyl-carrier-protein) S-malonyltransferase Length = 312 Score = 187 bits (474), Expect = 4e-47, Method: Compositional matrix adjust. Identities = 114/301 (37%), Positives = 166/301 (55%), Gaps = 3/301 (0%) Query: 2 SKIAFLFSGQGSQYVGMGKSLYDNFSIAKETYAEASEVLGFDLGRLCFEGSSSELSRTEN 61 + +AF+F GQGSQ +GM L ++ ++T+AEASE LG+DL L G L++T+ Sbjct: 3 ASLAFVFPGQGSQSLGMLAELGAQQALVRDTFAEASEALGYDLWALVQNGPEERLNQTDK 62 Query: 62 TQPAILAASIAQYRVYMQEIGEQPCYAAGHSLGEYSALAAAGAIEFADAVKLVRARGLYM 121 TQPAIL SIA +R+++ E G +P + AGHSLGEYSAL AA ++ FADAVKLV RG M Sbjct: 63 TQPAILTVSIALWRLWLAEGGARPAFVAGHSLGEYSALVAAESLAFADAVKLVERRGQLM 122 Query: 122 KXXXXXXXXXXXXIRDVEDSVIEEECRTLSAQGRIIGISNYNGNKDIVVSGEAQAVERLV 181 + I +ED+ + C +AQG ++ N+N +V++G A AVER + Sbjct: 123 QQAVPAGQGGMAAILGLEDADVLAACAE-AAQGEVVSAVNFNAPGQVVIAGAAAAVERAI 181 Query: 182 EVLKNQGAQ-IFKLNVSAAFHSPIMEPAAVKLNRELGNYNFSQPQYQVISNVSALPYEDK 240 E K +GA+ L VS H +M PAA + + + + P+ ++ NVSA D Sbjct: 182 EACKARGAKRAVALPVSVPSHCELMRPAAEQFAASVESLQWQAPKISLVQNVSAAVPADL 241 Query: 241 GQIRDILTQQIVNPVNWYATMKYLERNGIDTAIEFGPKATLRNLVRNNT-GIAAYSYDKQ 299 +R L Q+ +PV W +++ L G+ +E GP L L R GI + D Sbjct: 242 DTLRRDLLAQLYSPVRWVESIQLLAEKGVTELVECGPGKVLAGLNRRCAKGINTHGLDGV 301 Query: 300 E 300 E Sbjct: 302 E 302 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.130 0.357 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 27,864,207 Number of extensions: 1091676 Number of successful extensions: 1805 Number of sequences better than 1.0e-05: 40 Number of HSP's gapped: 1725 Number of HSP's successfully gapped: 40 Length of query: 418 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 309 Effective length of database: 25,807,025 Effective search space: 7974370725 Effective search space used: 7974370725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 117 (49.7 bits)