BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0382.0020.p (285 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGl2933_1 [C] COG0281 Malic enzyme 110 2e-24 AGl2936 [G] COG4993 Glucose dehydrogenase 110 2e-24 AGl2977 [S] COG3034 Uncharacterized protein conserved in bacteria 110 2e-24 AGl2992_1 [M] COG1596 Periplasmic protein involved in polysaccha... 110 2e-24 AGl2989 [M] COG2148 Sugar transferases involved in lipopolysacch... 110 2e-24 >AGl2933_1 [C] COG0281 Malic enzyme Length = 353 Score = 110 bits (275), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 86/296 (29%), Positives = 132/296 (44%), Gaps = 42/296 (14%) Query: 3 IADLSAKEADVHIHSALEAGIDFFDHADIYAAGKAEEVFGDVVASSPGMRDKLMIQTKCG 62 I D+ AD + ++++AGI+FFD AD+Y+AG +E G + R+++++ TK Sbjct: 33 IGDVGQTGADELVKASIDAGINFFDTADVYSAGASERTLGQSFKNLGIARNEIVLATKVY 92 Query: 63 IRQGF----FDFSKEHIVSSVENSLKRLKTDYVDVLLLHRPDTLMEPGEVAEAFDYLEQK 118 R G S+ HI+ +VE SLKRL+TD++D+ +H DTL E A D L ++ Sbjct: 93 SRMGSGRNDVGASRGHIMDAVEASLKRLQTDHIDLYQIHATDTLTPVEETLRALDDLVRQ 152 Query: 119 GMVKHFGVSNQNPLQIELLKKNVKQKL---LFNQLQLSIMVSGMIDSGFNVNMTNSASVV 175 G V++ GVSN +I V +L FN LQ ++G ++ + Sbjct: 153 GKVRYVGVSNWQAWRIA-TALGVSARLGLERFNTLQAYYSIAGR-------DLERELKPL 204 Query: 176 HDGGILEYSRLHDMTIQPWSPFQYGFFEGVFLGNDKFPEVNK------------------ 217 D M + WSP G G F ++ PE ++ Sbjct: 205 LDA--------EKMGLLVWSPLAGGLLSGRFSRANQSPEGSRRSSFDFPIVDKERAWNVI 256 Query: 218 -VINRLADEKGVADTAIAIAWLLRHPAKMQPIVGTTNTQRLLDIAKASDITLSREE 272 V+ +A G + IA+AWLL P IVG +L D A LS +E Sbjct: 257 DVLKPIAKAHGCSPARIALAWLLAKPVVTSVIVGAKRLSQLEDNIAAVGTVLSDDE 312 >AGl2936 [G] COG4993 Glucose dehydrogenase Length = 353 Score = 110 bits (275), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 86/296 (29%), Positives = 132/296 (44%), Gaps = 42/296 (14%) Query: 3 IADLSAKEADVHIHSALEAGIDFFDHADIYAAGKAEEVFGDVVASSPGMRDKLMIQTKCG 62 I D+ AD + ++++AGI+FFD AD+Y+AG +E G + R+++++ TK Sbjct: 33 IGDVGQTGADELVKASIDAGINFFDTADVYSAGASERTLGQSFKNLGIARNEIVLATKVY 92 Query: 63 IRQGF----FDFSKEHIVSSVENSLKRLKTDYVDVLLLHRPDTLMEPGEVAEAFDYLEQK 118 R G S+ HI+ +VE SLKRL+TD++D+ +H DTL E A D L ++ Sbjct: 93 SRMGSGRNDVGASRGHIMDAVEASLKRLQTDHIDLYQIHATDTLTPVEETLRALDDLVRQ 152 Query: 119 GMVKHFGVSNQNPLQIELLKKNVKQKL---LFNQLQLSIMVSGMIDSGFNVNMTNSASVV 175 G V++ GVSN +I V +L FN LQ ++G ++ + Sbjct: 153 GKVRYVGVSNWQAWRIA-TALGVSARLGLERFNTLQAYYSIAGR-------DLERELKPL 204 Query: 176 HDGGILEYSRLHDMTIQPWSPFQYGFFEGVFLGNDKFPEVNK------------------ 217 D M + WSP G G F ++ PE ++ Sbjct: 205 LDA--------EKMGLLVWSPLAGGLLSGRFSRANQSPEGSRRSSFDFPIVDKERAWNVI 256 Query: 218 -VINRLADEKGVADTAIAIAWLLRHPAKMQPIVGTTNTQRLLDIAKASDITLSREE 272 V+ +A G + IA+AWLL P IVG +L D A LS +E Sbjct: 257 DVLKPIAKAHGCSPARIALAWLLAKPVVTSVIVGAKRLSQLEDNIAAVGTVLSDDE 312 >AGl2977 [S] COG3034 Uncharacterized protein conserved in bacteria Length = 353 Score = 110 bits (275), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 86/296 (29%), Positives = 132/296 (44%), Gaps = 42/296 (14%) Query: 3 IADLSAKEADVHIHSALEAGIDFFDHADIYAAGKAEEVFGDVVASSPGMRDKLMIQTKCG 62 I D+ AD + ++++AGI+FFD AD+Y+AG +E G + R+++++ TK Sbjct: 33 IGDVGQTGADELVKASIDAGINFFDTADVYSAGASERTLGQSFKNLGIARNEIVLATKVY 92 Query: 63 IRQGF----FDFSKEHIVSSVENSLKRLKTDYVDVLLLHRPDTLMEPGEVAEAFDYLEQK 118 R G S+ HI+ +VE SLKRL+TD++D+ +H DTL E A D L ++ Sbjct: 93 SRMGSGRNDVGASRGHIMDAVEASLKRLQTDHIDLYQIHATDTLTPVEETLRALDDLVRQ 152 Query: 119 GMVKHFGVSNQNPLQIELLKKNVKQKL---LFNQLQLSIMVSGMIDSGFNVNMTNSASVV 175 G V++ GVSN +I V +L FN LQ ++G ++ + Sbjct: 153 GKVRYVGVSNWQAWRIA-TALGVSARLGLERFNTLQAYYSIAGR-------DLERELKPL 204 Query: 176 HDGGILEYSRLHDMTIQPWSPFQYGFFEGVFLGNDKFPEVNK------------------ 217 D M + WSP G G F ++ PE ++ Sbjct: 205 LDA--------EKMGLLVWSPLAGGLLSGRFSRANQSPEGSRRSSFDFPIVDKERAWNVI 256 Query: 218 -VINRLADEKGVADTAIAIAWLLRHPAKMQPIVGTTNTQRLLDIAKASDITLSREE 272 V+ +A G + IA+AWLL P IVG +L D A LS +E Sbjct: 257 DVLKPIAKAHGCSPARIALAWLLAKPVVTSVIVGAKRLSQLEDNIAAVGTVLSDDE 312 >AGl2992_1 [M] COG1596 Periplasmic protein involved in polysaccharide export Length = 353 Score = 110 bits (275), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 86/296 (29%), Positives = 132/296 (44%), Gaps = 42/296 (14%) Query: 3 IADLSAKEADVHIHSALEAGIDFFDHADIYAAGKAEEVFGDVVASSPGMRDKLMIQTKCG 62 I D+ AD + ++++AGI+FFD AD+Y+AG +E G + R+++++ TK Sbjct: 33 IGDVGQTGADELVKASIDAGINFFDTADVYSAGASERTLGQSFKNLGIARNEIVLATKVY 92 Query: 63 IRQGF----FDFSKEHIVSSVENSLKRLKTDYVDVLLLHRPDTLMEPGEVAEAFDYLEQK 118 R G S+ HI+ +VE SLKRL+TD++D+ +H DTL E A D L ++ Sbjct: 93 SRMGSGRNDVGASRGHIMDAVEASLKRLQTDHIDLYQIHATDTLTPVEETLRALDDLVRQ 152 Query: 119 GMVKHFGVSNQNPLQIELLKKNVKQKL---LFNQLQLSIMVSGMIDSGFNVNMTNSASVV 175 G V++ GVSN +I V +L FN LQ ++G ++ + Sbjct: 153 GKVRYVGVSNWQAWRIA-TALGVSARLGLERFNTLQAYYSIAGR-------DLERELKPL 204 Query: 176 HDGGILEYSRLHDMTIQPWSPFQYGFFEGVFLGNDKFPEVNK------------------ 217 D M + WSP G G F ++ PE ++ Sbjct: 205 LDA--------EKMGLLVWSPLAGGLLSGRFSRANQSPEGSRRSSFDFPIVDKERAWNVI 256 Query: 218 -VINRLADEKGVADTAIAIAWLLRHPAKMQPIVGTTNTQRLLDIAKASDITLSREE 272 V+ +A G + IA+AWLL P IVG +L D A LS +E Sbjct: 257 DVLKPIAKAHGCSPARIALAWLLAKPVVTSVIVGAKRLSQLEDNIAAVGTVLSDDE 312 >AGl2989 [M] COG2148 Sugar transferases involved in lipopolysaccharide synthesis Length = 353 Score = 110 bits (275), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 86/296 (29%), Positives = 132/296 (44%), Gaps = 42/296 (14%) Query: 3 IADLSAKEADVHIHSALEAGIDFFDHADIYAAGKAEEVFGDVVASSPGMRDKLMIQTKCG 62 I D+ AD + ++++AGI+FFD AD+Y+AG +E G + R+++++ TK Sbjct: 33 IGDVGQTGADELVKASIDAGINFFDTADVYSAGASERTLGQSFKNLGIARNEIVLATKVY 92 Query: 63 IRQGF----FDFSKEHIVSSVENSLKRLKTDYVDVLLLHRPDTLMEPGEVAEAFDYLEQK 118 R G S+ HI+ +VE SLKRL+TD++D+ +H DTL E A D L ++ Sbjct: 93 SRMGSGRNDVGASRGHIMDAVEASLKRLQTDHIDLYQIHATDTLTPVEETLRALDDLVRQ 152 Query: 119 GMVKHFGVSNQNPLQIELLKKNVKQKL---LFNQLQLSIMVSGMIDSGFNVNMTNSASVV 175 G V++ GVSN +I V +L FN LQ ++G ++ + Sbjct: 153 GKVRYVGVSNWQAWRIA-TALGVSARLGLERFNTLQAYYSIAGR-------DLERELKPL 204 Query: 176 HDGGILEYSRLHDMTIQPWSPFQYGFFEGVFLGNDKFPEVNK------------------ 217 D M + WSP G G F ++ PE ++ Sbjct: 205 LDA--------EKMGLLVWSPLAGGLLSGRFSRANQSPEGSRRSSFDFPIVDKERAWNVI 256 Query: 218 -VINRLADEKGVADTAIAIAWLLRHPAKMQPIVGTTNTQRLLDIAKASDITLSREE 272 V+ +A G + IA+AWLL P IVG +L D A LS +E Sbjct: 257 DVLKPIAKAHGCSPARIALAWLLAKPVVTSVIVGAKRLSQLEDNIAAVGTVLSDDE 312 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.136 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,817,549 Number of extensions: 785759 Number of successful extensions: 14389 Number of sequences better than 1.0e-05: 167 Number of HSP's gapped: 20395 Number of HSP's successfully gapped: 169 Length of query: 285 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 180 Effective length of database: 26,268,361 Effective search space: 4728304980 Effective search space used: 4728304980 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)