BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0382.0014.p (169 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0620 [K] COG1595 DNA-directed RNA polymerase specialized sigma... 106 1e-23 CAC3267 [K] COG1595 DNA-directed RNA polymerase specialized sigm... 75 7e-14 BS_sigV [K] COG1595 DNA-directed RNA polymerase specialized sigm... 73 2e-13 BH0263 [K] COG1595 DNA-directed RNA polymerase specialized sigma... 73 2e-13 BH2026 [K] COG1595 DNA-directed RNA polymerase specialized sigma... 72 6e-13 >BH0620 [K] COG1595 DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog Length = 177 Score = 106 bits (265), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 56/140 (40%), Positives = 84/140 (60%), Gaps = 2/140 (1%) Query: 29 EHRKLYAIAYSYLRSEADALEAVQEASCRAWMKRKKLKNEQAFTPWLIRITINCCMDELR 88 E KLY +A++Y+R+E DAL+ VQEA + + ++K+ F+ W+ RI IN +D LR Sbjct: 23 EKDKLYRVAFTYVRNEHDALDVVQEAVVKGYEAYDRIKHLDYFSSWMTRIVINTAIDLLR 82 Query: 89 RKKRVFP--AEKLEEKETQEMRSSDRLDLERAMNRMKPKYRHVVMLKYYQDMTSAEIAKV 146 + K V P EK + E RLDL ++ +KP+ +++L+YY D T EIA+ Sbjct: 83 KGKDVIPFCPEKSQMNGWTETHIISRLDLFDVLSELKPEETTLLVLRYYHDFTILEIARF 142 Query: 147 LNKPEGTIKTWLREGLKQLR 166 +NKPEGTIK L L ++R Sbjct: 143 MNKPEGTIKAQLHRTLAKVR 162 >CAC3267 [K] COG1595 DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog Length = 190 Score = 74.7 bits (182), Expect = 7e-14, Method: Compositional matrix adjust. Identities = 43/155 (27%), Positives = 79/155 (50%), Gaps = 16/155 (10%) Query: 18 DEEVFFQRLAGEHRKLYAIAYSYLRSEADALEAVQEASCRAWMKRKKLKNEQAFTPWLIR 77 D F Q + LY + Y Y ++ +DA++ VQE + + K F PW+ + Sbjct: 15 DRASFSQLFHLYEKYLYNLCYGYTQNTSDAMDLVQEIYIKVFKNIKNFDENLPFHPWVRK 74 Query: 78 ITINCCMDELRRKK--------RVFPAE-----KLEEKETQEMRSSDRLD---LERAMNR 121 I++N C++ R + + + +E +L E E +R++ ++ ++ + Sbjct: 75 ISVNTCLNFKRDNRGNVMSLNSKYYDSEFEEEDRLAEDYNMEQEVVNRINNEVVKESIKK 134 Query: 122 MKPKYRHVVMLKYYQDMTSAEIAKVLNKPEGTIKT 156 + YR V+ML+YY+D++ EIA VL+ P GT+KT Sbjct: 135 LTYDYRMVIMLRYYEDLSYDEIASVLDMPIGTVKT 169 >BS_sigV [K] COG1595 DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog Length = 166 Score = 73.2 bits (178), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 39/145 (26%), Positives = 84/145 (57%), Gaps = 11/145 (7%) Query: 29 EHRK-LYAIAYSYLRSEADALEAVQEASCRAWMKRKKLKNEQAFTPWLIRITINCCMDEL 87 +H++ Y +A+SY++++ DAL+ VQE+ +A + ++N + W +I + +D L Sbjct: 17 DHKQDFYRLAFSYVKNQDDALDIVQESIKKALSSVETVRNPETIKSWFYKILVRTAIDFL 76 Query: 88 RRKKRVFPAEKLEEKETQEMRSSDR------LDLERAMNRMKPKYRHVVMLKYYQDMTSA 141 R++K++ ++ + ET E S + DL A++ + +Y+ +++L++++D+ Sbjct: 77 RKQKKI----RVMDDETIEFLSKGKEDHYKDTDLHEALDELPYRYKTIIILRFFEDLKLE 132 Query: 142 EIAKVLNKPEGTIKTWLREGLKQLR 166 EIA++ + T+KT L LK +R Sbjct: 133 EIAEITGENTNTVKTRLYRALKLMR 157 >BH0263 [K] COG1595 DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog Length = 187 Score = 72.8 bits (177), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 47/166 (28%), Positives = 81/166 (48%), Gaps = 17/166 (10%) Query: 18 DEEVFFQRLAGEHRKLYAIAYSYLRSEADALEAVQEASCRAWMKRKKLKNEQAFTPWLIR 77 DE+ F + + K+Y +AY + + +A + QEA RA+ ++ F+ WL R Sbjct: 16 DEQAFAELVELYKDKVYQVAYRMVGNVHEAQDVAQEAFLRAYTNIDTYDIQRKFSTWLFR 75 Query: 78 ITINCCMDELRRKKRVFPAEKL-----------------EEKETQEMRSSDRLDLERAMN 120 I N +D LR++K F E E + Q ++ R +++ + Sbjct: 76 IATNVAIDRLRKRKPDFHLEDPVQGADGLTIESQLAADEEPVDDQVIQMETRDWIQQEIQ 135 Query: 121 RMKPKYRHVVMLKYYQDMTSAEIAKVLNKPEGTIKTWLREGLKQLR 166 + PKYR V+LKY +D++ EI+ +L+ P T+KT + G + LR Sbjct: 136 ALPPKYRMAVLLKYMEDLSLKEISDILDLPVSTVKTRIHRGREALR 181 >BH2026 [K] COG1595 DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog Length = 179 Score = 71.6 bits (174), Expect = 6e-13, Method: Compositional matrix adjust. Identities = 40/160 (25%), Positives = 84/160 (52%), Gaps = 8/160 (5%) Query: 18 DEEVFFQRLAGEHRKLYAIAYSYLRSEADALEAVQEASCRAWMKRKKLKNEQAFTPWLIR 77 ++E Q + E + Y +A +Y+ + DA++A+++ + +L+ E AF W Sbjct: 14 NKEALLQLVMAEKDRYYRLALTYMGNPHDAMDAMEDMIVTLYETIDQLQKEDAFYSWSNT 73 Query: 78 ITINCCMDELRRKKRVFPAEKLEEKETQE--------MRSSDRLDLERAMNRMKPKYRHV 129 I +N C L +++++ A++ E+ET ++ LD+ R ++ + + + Sbjct: 74 ILVNRCKRMLSKQQKLIHAKEWNEEETNRYSTFIDPFIQREQHLDIMRLLDHLNEQQKEA 133 Query: 130 VMLKYYQDMTSAEIAKVLNKPEGTIKTWLREGLKQLRNYL 169 + LKY+ D+ IA + N GT+K+ + +GLK+LR ++ Sbjct: 134 IQLKYFHDLDYQTIANMTNVSVGTVKSRVFQGLKKLREHI 173 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.131 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 11,225,484 Number of extensions: 406217 Number of successful extensions: 1592 Number of sequences better than 1.0e-05: 41 Number of HSP's gapped: 1575 Number of HSP's successfully gapped: 41 Length of query: 169 Length of database: 38,378,431 Length adjustment: 99 Effective length of query: 70 Effective length of database: 26,960,365 Effective search space: 1887225550 Effective search space used: 1887225550 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 112 (47.8 bits)