BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0377.0031.p (337 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value TM0067 [G] COG0524 Sugar kinases, ribokinase family 181 1e-45 CAC2684 [G] COG0524 Sugar kinases, ribokinase family 169 6e-42 VNG0158G [G] COG0524 Sugar kinases, ribokinase family 104 2e-22 CC1496 [G] COG0524 Sugar kinases, ribokinase family 103 4e-22 STM3612 [G] COG0524 Sugar kinases, ribokinase family 71 3e-12 >TM0067 [G] COG0524 Sugar kinases, ribokinase family Length = 339 Score = 181 bits (460), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 105/334 (31%), Positives = 167/334 (50%), Gaps = 12/334 (3%) Query: 3 RIAAFGEVMMRLQVPGYETLVQSSRLEYSFSGTGVNVTAALARYGHNGAIITTLPETPLG 62 ++ FGE+M+RL P ++ + Q+ + ++ G NV A LA+ G + +T LP PLG Sbjct: 2 KVVTFGEIMLRLSPPDHKRIFQTDSFDVTYGGAEANVAAFLAQMGLDAYFVTKLPNNPLG 61 Query: 63 DAAVAYLRKLGIDTSLVSRGGKHLGMYFLENGFGVRPGRVTYTDRLGSSFNTAAESNYDM 122 DAA +LRK G+ T ++RGG +G+YFLE G RP +V Y DR S+ + A ++D Sbjct: 62 DAAAGHLRKFGVKTDYIARGGNRIGIYFLEIGASQRPSKVVY-DRAHSAISEAKREDFDW 120 Query: 123 AALASRVDVLHLCGITLAMNDEVRRQMKRLAAEVKSAGGKVVFDCNYRPSLWGPDGYALA 182 + H GIT + E+ ++ G V D NYR LW + A Sbjct: 121 EKILDGARWFHFSGITPPLGKELPLILEDALKVANEKGVTVSCDLNYRARLWTKEE---A 177 Query: 183 RPHYEELLQLADTVMMNEQDARFILGIG-------TGEYDRITQLKHAIPEVVKRFGIGT 235 + ++ D ++ NE+D +LGI TG+ +R K A EV +++ T Sbjct: 178 QKVMIPFMEYVDVLIANEEDIEKVLGISVEGLDLKTGKLNREAYAKIA-EEVTRKYNFKT 236 Query: 236 AAGTHREINDNNTHSLTGYIYHQGRFEFSRKLAFPVLDRIGAGDAFASAVIHGELQQYPP 295 T RE + + ++ G+ FS + ++DR+GAGD+FA A+I+G L + Sbjct: 237 VGITLRESISATVNYWSVMVFENGQPHFSNRYEIHIVDRVGAGDSFAGALIYGSLMGFDS 296 Query: 296 QQTVNMAGGAAMLAHTIVGDTTLFTEGEVLRALS 329 Q+ A A+ L HTI GD + + E+ + S Sbjct: 297 QKKAEFAAAASCLKHTIPGDFVVLSIEEIEKLAS 330 >CAC2684 [G] COG0524 Sugar kinases, ribokinase family Length = 329 Score = 169 bits (428), Expect = 6e-42, Method: Compositional matrix adjust. Identities = 100/329 (30%), Positives = 164/329 (49%), Gaps = 9/329 (2%) Query: 1 MPRIAAFGEVMMRLQVPGYETLVQSSRLEYSFSGTGVNVTAALARYGHNGAIITTLPETP 60 M RI GE+M+RL GY VQ+ + + G+ NV +LA +G + + T LP+ Sbjct: 1 MGRIVTLGEIMLRLSPQGYNRFVQAKQFNAFYGGSEANVAVSLAVFGKDVSYTTKLPDNA 60 Query: 61 LGDAAVAYLRKLGIDTSLVSRGGKHLGMYFLENGFGVRPGRVTYTDRLGSSFNTAAESNY 120 + A+ LRK G++T + RGG +G+YF ENG RP ++ Y DR S+ A ++ Sbjct: 61 IAQCAINELRKYGVNTKDIVRGGDRIGLYFCENGESQRPAKLVY-DRKDSAIAKAKREDF 119 Query: 121 DMAALASRVDVLHLCGITLAMNDEVRRQMKRLAAEVKSAGGKVVFDCNYRPSLWGPDGYA 180 + + D H GIT A++DE + K+ G V D NYR +LW ++ Sbjct: 120 NWDKIFDGADWFHFSGITPALSDECAKITLDAVKAAKAKGLTVSIDLNYRENLWDAAAFS 179 Query: 181 LARPHYEELLQLADTVMMNEQDARFILGIGTGEYDRITQLKHAIPEVVKRFGIGTAAGTH 240 A ELLQ D + + ++A +G G G+ D T LK ++V++F + A Sbjct: 180 KA---IRELLQYCDVALGSIEEAELAIGEGDGD-DCNTVLK----KLVEKFQLKYATIIL 231 Query: 241 REINDNNTHSLTGYIYHQGRFEFSRKLAFPVLDRIGAGDAFASAVIHGELQQYPPQQTVN 300 R T +Y +F ++K V+D IG GDAFA+++I+ + Q+ + Sbjct: 232 RNRFTAEDVDWTAVLYDGSKFYNAKKYDIHVVDNIGGGDAFAASLIYAITSNFSNQEIIE 291 Query: 301 MAGGAAMLAHTIVGDTTLFTEGEVLRALS 329 A + L +++ GD L T E+++ ++ Sbjct: 292 FATAGSCLKYSMNGDVNLSTVDEIIKLMN 320 >VNG0158G [G] COG0524 Sugar kinases, ribokinase family Length = 318 Score = 104 bits (260), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 95/336 (28%), Positives = 135/336 (40%), Gaps = 22/336 (6%) Query: 4 IAAFGEVMMRLQVPGYETLVQSSRLEYSFSGTGVNVTAALARYGHNGAIITTLPETPLGD 63 + FGE M+RL PG+E + + R++ +G NV R G A ++ LP +PLG Sbjct: 3 VVTFGETMLRLSPPGHERVEAADRMDVRVAGAESNVAVTAGRLGAETAWLSKLPASPLGR 62 Query: 64 AAVAYLRKLGI--DTSLVSRGGKHLGMYFLENGFGVRPGRVTYTDRLGSSFNTAAESNYD 121 A LR G + + R G+YFLE G G G + DR ++ TA Sbjct: 63 KVAATLRAQGTTPEVTWTDRDDLRQGLYFLEQG-GEPRGTTVHYDRQDAAVTTATADEL- 120 Query: 122 MAALASRVDVLHLCGITLAMNDEVRRQMKRLAAEVKSAGGKVVFDCNYRPSLWGPDGYAL 181 A R LH GIT A+++ + L + G D NYR LW + Sbjct: 121 APAFVERAGYLHTSGITPALSETLAATTADLLELAQETGTTATLDVNYRRGLW---SHEE 177 Query: 182 ARPHYEELLQLADTVMMNEQDARFILGIGTGEYDRITQLKHAIPEVVKRFGIGTAAGTHR 241 AR E L D + + E+DAR +L TG+ D I + + F GT T Sbjct: 178 ARDTLEALFPDVDVLFVAERDARGVLD-RTGDADAIAR------GLADEFAFGTVVLTRG 230 Query: 242 EINDNNTHSLTGYIYHQGRFEFSRKLAFPVLDRIGAGDAFASAVIHGELQQYPPQQTVNM 301 E + T Q FE D IG GDAF + + + + Sbjct: 231 EAGALAVRNAT--TVTQPVFETETH------DPIGTGDAFVGGYLAARVDGGDVEDALAW 282 Query: 302 AGGAAMLAHTIVGDTTLFTEGEVLRALSEHTLDVER 337 A L TI GD L T +V L++ ++R Sbjct: 283 GAATAALKRTIPGDVALVTRADVESVLADEHSGIDR 318 >CC1496 [G] COG0524 Sugar kinases, ribokinase family Length = 368 Score = 103 bits (257), Expect = 4e-22, Method: Compositional matrix adjust. Identities = 96/343 (27%), Positives = 144/343 (41%), Gaps = 27/343 (7%) Query: 4 IAAFGEVMMRLQVPGYETLVQSSRLEYSFSGTGVNVTAALAR-YGHNGAIITTLPETPLG 62 A+FGEVM+R PG+ + + + + G NV A + +G +T LP LG Sbjct: 18 CASFGEVMLRFD-PGFGRVRNARQFQVWEGGGEYNVARAFKKCWGKRSTAVTALPVNDLG 76 Query: 63 DAAVAYLRKLGIDTS-LVSRG------GKHLGMYFLENGFGVRPGRVTYTDRLGSSFNT- 114 + + G+DTS ++ R +G+ F E GFGVRP + +DR S+ + Sbjct: 77 WLVEDLMMQGGVDTSHIIWRDFDGLGRNTRVGLNFTEKGFGVRPA-LGCSDRGHSAASQI 135 Query: 115 -AAESNYDMAALASRVDVLHLCGITLAMNDEVRRQMKRLAAEVKSAGGKVVFDCNYRPSL 173 E N++ V H GI A+ + + G V +D NYR SL Sbjct: 136 RPGEVNWEKLFGEEGVRWFHTGGIFAALASNTAEAVIEAVEVARKYGTVVSYDLNYRASL 195 Query: 174 W----GPDGYALARPHYEELLQLADTVMMNEQDARFILGIGTGEYDR------ITQLKHA 223 W G +G H + Q D ++ NE+D LG D K Sbjct: 196 WKSQGGKEGAQKVNRH---IAQYVDVMIGNEEDFTACLGFEVEGLDEHISSIDPANFKKM 252 Query: 224 IPEVVKRF-GIGTAAGTHREINDNNTHSLTGYIYHQGRFEFSR-KLAFPVLDRIGAGDAF 281 I VK+F AA T R + + + +Y G+F S + + DR+G GD F Sbjct: 253 IETAVKQFPNFKVAATTLRNAKTASVNDWSAILYAGGQFYASMMRENLEIYDRVGGGDGF 312 Query: 282 ASAVIHGELQQYPPQQTVNMAGGAAMLAHTIVGDTTLFTEGEV 324 AS + +G ++ PQ V LA T GDT++ + EV Sbjct: 313 ASGLAYGFMEGKGPQAAVEYGAAHGALAMTTPGDTSMVRKEEV 355 >STM3612 [G] COG0524 Sugar kinases, ribokinase family Length = 309 Score = 70.9 bits (172), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 68/290 (23%), Positives = 119/290 (41%), Gaps = 34/290 (11%) Query: 3 RIAAFGEVMMRLQVPGYETLVQSSRLEYSFSGTGVNVTAALARYGHNGAI----ITTLPE 58 +IA GE M+ L G + ++ F G +N + +AR + A+ +T L Sbjct: 4 KIAVIGECMIELSQKGAD-------VQRGFGGDTLNTSVYIARQVDSAALAVHYVTALGT 56 Query: 59 TPLGDAAVAYLRKLGIDTSLVSRGGKHL-GMYFLENGFGVRPGRVTYTDRLGSSFNTAAE 117 + + +DTSL R L G+Y++E + + + F +E Sbjct: 57 DSFSQQMLEAWQHENVDTSLTQRMENRLPGLYYIETDDTGERTFYYWRNEAAAKFWLESE 116 Query: 118 SNYDMAALASRVDVLHLCGITLA-MNDEVRRQMKRLAAEVKSAGGKVVFDCNYRPSLWGP 176 + + + D L+L GI+LA ++ R ++ L E ++ GGKV+FD NYRP LW Sbjct: 117 QSAAICETLATFDYLYLSGISLAILSPTSRDKLLSLLRECRANGGKVIFDNNYRPRLWT- 175 Query: 177 DGYALARPHYEELLQLADTVMMNEQDARFILGIGTGEYDRITQLKHAIPEVVKRFGIGTA 236 + Y+++L+ D + D + G + + EV+ R A Sbjct: 176 -SREETQQVYQKMLECTDIAFLTLDDEDALWG------------QQPVEEVIAR---THA 219 Query: 237 AGTHREINDNNTHSLTGYIYHQGRFEFSRKLAFP---VLDRIGAGDAFAS 283 AG + S I + + + P V+D AGD+F++ Sbjct: 220 AGVQEVVVKRGADSCLVSIQGEALIDVP-AVKLPKEKVIDTTAAGDSFSA 268 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.136 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 26,025,758 Number of extensions: 1058041 Number of successful extensions: 3496 Number of sequences better than 1.0e-05: 28 Number of HSP's gapped: 3480 Number of HSP's successfully gapped: 28 Length of query: 337 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 230 Effective length of database: 26,037,693 Effective search space: 5988669390 Effective search space used: 5988669390 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)