BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0377.0029.p
         (347 letters)

Database: COG 
           115,334 sequences; 38,378,431 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

STM4446 [E] COG1921 Selenocysteine synthase [seryl-tRNASer selen...   311   7e-85
STM3768 [E] COG1921 Selenocysteine synthase [seryl-tRNASer selen...   276   3e-74

>STM4446 [E] COG1921 Selenocysteine synthase [seryl-tRNASer selenium
           transferase]
          Length = 432

 Score =  311 bits (798), Expect = 7e-85,   Method: Compositional matrix adjust.
 Identities = 19/347 (5%), Positives = 55/347 (15%), Gaps = 3/347 (0%)

Query: 1   MSILGVSAPTDTVMEAMKQGGQQYVEIADLVDKSGDYIARLLGSEXXXXXXXXXXGIALS 60
                V      V  A    G        L  + GD    L                   
Sbjct: 20  TVEAEVHLEGGFVGMAAAPSGASTGSREALELRDGDKSRFLGKGVTKAVAAVNGPIAQAL 79

Query: 61  VAAIVTGGDPRLSLRLHQEPVLKNEIIMLKGHNVQYGAPVETMVFLGGGRVVEAGYANEG 120
           +            + +  +                  A  +      G  + E      G
Sbjct: 80  IGKDAKDQAGIDKIMIDLDGTENKSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNG 139

Query: 121 RKEHIEQAICERTAAILYVKSHHTVQKNMISVEEAWEVAARSGVPLIVDAAAEEDLLKYV 180
                   +           + + V      ++       +  + +  +       +   
Sbjct: 140 TPGKYSMPVPMMNIINGGEHADNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKA 199

Query: 181 QY-SDLAIYSGSKAIEGPTSGIVAGKRKYVEWVKVQLHGIGRSMKVGKETTFGLLQALEE 239
           +  +      G  A    ++                  G   ++ +    +         
Sbjct: 200 KGMNTAVGDEGGYAPNLGSNAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYV 259

Query: 240 Y--QDKPDNSEVEKKALEALQPLGTLHGVSVRIVQDEAGRAIFRGRIQIDAAAAGVDAKT 297
              +     +  E                    + +            +      V    
Sbjct: 260 LAGEGNKAFTSEEFTHFLEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDL 319

Query: 298 VNDQLREGEIAVYTRDYGVKQGYFDIDPRSLQGDDLQVIIDRIHEIV 344
                +  +  +           F+      +      +        
Sbjct: 320 FVTNTKILKEGIEKGIANSILIKFNQIGSLTETLAAIKMAKDAGYTA 366


>STM3768 [E] COG1921 Selenocysteine synthase [seryl-tRNASer selenium
           transferase]
          Length = 211

 Score =  276 bits (706), Expect = 3e-74,   Method: Compositional matrix adjust.
 Identities = 0/350 (0%), Positives = 0/350 (0%), Gaps = 10/350 (2%)

Query: 1   MSILGVSAPTDTVMEAMKQGGQQYVEIADLVDKSGDYIARLLGSEXXXXXXXXXXGIALS 60
            + L  S+P + ++  +       V +     K                         + 
Sbjct: 21  TTELNFSSPFELLIAVLLSAQATDVSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIG 80

Query: 61  VAAIVTGGDPRLSLRLHQEPVLKNEIIMLKGHNVQYGAPVETMVFLGGGRVVEAGYANEG 120
           +         +    L ++   +          +         V L           +  
Sbjct: 81  LYNSKAENIIKTCRILLEQHNGEVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTH 140

Query: 121 RKEHIEQAICERTAAILYVKSHHTVQKNMISVEEAWEVAARSGVPLIVDAAAEEDLLKYV 180
                 +        +  V+             +        G    +            
Sbjct: 141 IFRVCNRTQFAPGKNVEQVEEKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIE 200

Query: 181 QY-SDLAIYSGSKAIEGPTSGIVAGKRKYVEWVKVQLHGIGRSMKVGKETTFGLLQALEE 239
                                                                       
Sbjct: 201 DLC                                                          260

Query: 240 YQDKPDNSEVEKKALEALQP----LGTLHGVSVRIVQDEAGRAIFRGRIQIDAAAAGVDA 295
                                                                       
Sbjct: 261 -----                                                        315

Query: 296 KTVNDQLREGEIAVYTRDYGVKQGYFDIDPRSLQGDDLQVIIDRIHEIVE 345
                                                             
Sbjct: 316                                                    365


  Database: COG
    Posted date:  Aug 3, 2010  9:58 AM
  Number of letters in database: 38,378,431
  Number of sequences in database:  115,334
  
Lambda     K      H
   0.316    0.135    0.375 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 115334
Number of Hits to DB: 25,449,015
Number of extensions: 964978
Number of successful extensions: 2817
Number of sequences better than 1.0e-05: 3
Number of HSP's gapped: 2815
Number of HSP's successfully gapped: 3
Length of query: 347
Length of database: 38,378,431
Length adjustment: 107
Effective length of query: 240
Effective length of database: 26,037,693
Effective search space: 6249046320
Effective search space used: 6249046320
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 116 (49.3 bits)