BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0377.0020.p (536 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2122 [G] COG0383 Alpha-mannosidase 506 e-143 BH0789 [G] COG0383 Alpha-mannosidase 373 e-103 lin0424 [G] COG0383 Alpha-mannosidase 201 3e-51 lin0449 [G] COG0383 Alpha-mannosidase 140 6e-33 BH0788 [G] COG0383 Alpha-mannosidase 123 8e-28 >lin2122 [G] COG0383 Alpha-mannosidase Length = 882 Score = 506 bits (1304), Expect = e-143, Method: Compositional matrix adjust. Identities = 250/495 (50%), Positives = 342/495 (69%), Gaps = 14/495 (2%) Query: 1 MPYERHHVLLAKLMNELLETLEKDEHYRYFHLDGQTIIIEDYLQIHPEKKEILERFIREG 60 +P E L LM+E+ + L+K+ + +FH+DGQ I++EDYL + P K+E +++ + +G Sbjct: 20 LPLESLRFRLVTLMDEVEDLLDKEAGFNHFHMDGQMIMLEDYLAVKPGKREKMKQLVADG 79 Query: 61 RIVIGPWYVLQDEFLTSSEANVRNLLTGHQDAAKYGVISKLGYFPDSFGNMGQAPQLLRQ 120 ++ IGPWY+LQD FLTS EAN+RNL G + A ++G K+GYFPD+FG GQ PQL+RQ Sbjct: 80 KLRIGPWYMLQDAFLTSGEANIRNLQYGLEMAEEFGQAEKIGYFPDTFGLYGQVPQLMRQ 139 Query: 121 ADIETAVFGRGVKPTGFDNMVGELNSASYESPYSEMFWESPDGSRVLGLLFANWYSNGNE 180 A +T VFGRGV PTGF+N V + +++ S YSEMFWESPDGS+VLG+L ANWYSNGNE Sbjct: 140 AGFDTVVFGRGVNPTGFNNQVFD---SAFASKYSEMFWESPDGSKVLGILLANWYSNGNE 196 Query: 181 VPAGPEEAKVFWDKKLADAGKYASTPELLFMNGCDHQPVQRNLSDALETAKALYPDVDFV 240 +P E AK+FWDKKLAD ++AST E LFMNGCDHQPVQ +L++ALE A+ LYP++DF+ Sbjct: 197 IPTDKEAAKIFWDKKLADVERFASTDEWLFMNGCDHQPVQTDLAEALEVARELYPEIDFI 256 Query: 241 HSSFEQYLEALKPSL-PANLVIVNGELRSQHTDGWGTLVNTASARIYLKQMNQRGQTLLE 299 HS FE+Y +A+K L P L V+GEL SQ +DGW TL NTAS+RIYLKQ N + + LLE Sbjct: 257 HSHFEKYQDAVKAGLEPEKLQTVHGELTSQQSDGWSTLANTASSRIYLKQANAKIERLLE 316 Query: 300 KGAEPLATLAYLASGQAYPHQLLNYAWKTLMQNHPHDSICGCSVDEVHREMTGRFEKSRH 359 + AEP++ +A A G +YPH+ L +AWK LM+N HDSI GCS+DEVHREM RFEK Sbjct: 317 RLAEPISVMATEA-GVSYPHEYLAFAWKLLMENQIHDSITGCSLDEVHREMEARFEKVEQ 375 Query: 360 VGEAIIEESLKAISGQIGTSGVACWGASGIPVTVFNTTGWDRTGTVSVEVIAAKRYFKEG 419 AII+ + + I+ QI T+ +GIP+TVF+ G + T TV +E+ +F E Sbjct: 376 TTMAIIQNAARKITEQIVTND------AGIPITVFHAGGVEATRTVELELETDAIHFSEM 429 Query: 420 PNPAAIAEALDQLPLALDSGRLVDADGQVLACRAEDMGTRFGYELPDDQFRRPYMARTLR 479 + I + L +LP S R+ ++G+ + E +G F Y+LP+ +FR Y AR + Sbjct: 430 -HFELIPDELAKLP--EQSFRMETSNGEEIPVVVEPLGIHFNYDLPERKFRDSYFARKYK 486 Query: 480 LTFEAAQVPPLGYKT 494 LTF +P +GY+T Sbjct: 487 LTFTVEHLPAVGYQT 501 >BH0789 [G] COG0383 Alpha-mannosidase Length = 777 Score = 373 bits (958), Expect = e-103, Method: Compositional matrix adjust. Identities = 195/393 (49%), Positives = 243/393 (61%), Gaps = 12/393 (3%) Query: 101 LGYFPDSFGNMGQAPQLLRQADIETAVFGRGVKPTGFDNMVGELNSASYESPYSEMFWES 160 + FPD+FG GQAPQLL QA I AVFGRGV PTGF+N V Y SP+SE+ WE+ Sbjct: 1 MATFPDTFGIYGQAPQLLAQAGIRAAVFGRGVTPTGFNNQV---QHDDYSSPFSELIWEA 57 Query: 161 PDGSRVLGLLFANWYSNGNEVPAGPEEAKVFWDKKLADAGKYASTPELLFMNGCDHQPVQ 220 PDGS+V+G+L ANWYSNGNE+P +EA+ FW KKL DA ++AST +LLFMNGCDHQPVQ Sbjct: 58 PDGSQVIGILLANWYSNGNEIPTDEDEAQTFWVKKLRDAERFASTSQLLFMNGCDHQPVQ 117 Query: 221 RNLSDALETAKALYPDVDFVHSSFEQYLEALKPSLPANLVIVNGELRSQHTDGWGTLVNT 280 ++++ A++ A+ L+PDV F HS+F YL +K LP L + GELR+Q TDGW TLVNT Sbjct: 118 KDVTQAIKVAETLFPDVAFKHSNFHDYLTQIKEELPKELQKITGELRNQKTDGWSTLVNT 177 Query: 281 ASARIYLKQMNQRGQTLLEKGAEPLATLAYLASGQAYPHQLLNYAWKTLMQNHPHDSICG 340 ASARIYLKQ N R QTLL EP + L ++ Y WK LM+NHPHDSICG Sbjct: 178 ASARIYLKQANDRCQTLLTNVLEP---MCLLVENKSLHRDFSEYYWKLLMENHPHDSICG 234 Query: 341 CSVDEVHREMTGRFEKSRHVGEAIIEESLKAISGQIGTSGVACWGASGIPVTVFNTTGWD 400 CS+D VHREM RFEK I E K I+ QI T + IP+ V T G Sbjct: 235 CSIDAVHREMKTRFEKVEAGATTFIAEQGKEIAAQINTLHDS---EEAIPLVVLKTNGTS 291 Query: 401 RTGTVSVEVIAAKRYFKEGPNPAAIAEALDQLPLALDSGRLVDADGQVLACRAEDMGTRF 460 V +V K YF E + I + L + + + + RL + + +D G RF Sbjct: 292 GKRVVRHKVAMKKIYFDEM-DFRHIPDRLKE--IVMPTYRLEFPNKGSVPIEVQDAGVRF 348 Query: 461 GYELPDDQFRRPYMARTLRLTFEAAQVPPLGYK 493 GY+LP D FRRPY AR L +TF LGY+ Sbjct: 349 GYDLPRDGFRRPYYARELEVTFSYDSDLYLGYE 381 >lin0424 [G] COG0383 Alpha-mannosidase Length = 875 Score = 201 bits (511), Expect = 3e-51, Method: Compositional matrix adjust. Identities = 123/405 (30%), Positives = 208/405 (51%), Gaps = 29/405 (7%) Query: 5 RHHVLLAKLMNELLETLEKDEHYRYFHLDGQTIIIEDYLQIHPEKKEILERFIREGRIVI 64 R + L K + E++ETLE ++Y ++ +D Q+ +IEDYL+ PE K LE+ I E R++ Sbjct: 24 RSTIYLVKHLKEVIETLEAKDNYHFYLMDAQSSLIEDYLRYCPEDKTRLEKLIAEKRLIT 83 Query: 65 GPWYVLQDEFLTSSEANVRNLLTGHQDAAKYGVISKLGYFPDSFGNMGQAPQLLRQADIE 124 GPWY D+ + S E+ VRNLL G + A + G +GY PD+FG G PQ+ ++ I Sbjct: 84 GPWYTQTDQLVISQESIVRNLLYGTRIAREMGHSMAVGYVPDAFGQGGNMPQIYKEFGIS 143 Query: 125 TAVFGRGVKPTGFDNMVGELNSASYESPYSEMFWESPDGSRVLGLLFANWYSNGNEVPAG 184 +F RGV DN + + +E W DG+ +L Y G +P Sbjct: 144 KFLFWRGVA----DNRLKQ----------TEFIWRGDDGTEMLAEQIPFGYYYGANIPEN 189 Query: 185 PEEAKVFWDKKLADAGKYASTPELLFMNGCDHQPVQRNLSDALETAKALYPDVDFVHSSF 244 E K + D+++ + ASTP + F NG D PV++NL + + L ++ +S Sbjct: 190 EAELKTYLDEQIGALEEKASTPNVYFPNGLDQAPVRKNLPELVAKFNELDHSREYQIASP 249 Query: 245 EQYLEALKPSLPANLVIVNGELRSQHTDGWGTLVNTA--SARIYLKQMNQRGQTLLEKGA 302 E + L+ + +L ++ GEL T+G + ++ + S R LKQ N + L Sbjct: 250 ETFFADLEKDV-TDLPVIAGEL----TEGKHSRLHKSIFSTRADLKQANNEIENFLSNVL 304 Query: 303 EPLATLAYLASGQAYPHQLLNYAWKTLMQNHPHDSICGCSVDEVHREMTGRFEKSRHVGE 362 EP+ +++Y + G YPH L WK + +N HDSI GC+ D +R++ R++ + + Sbjct: 305 EPVLSISY-SLGNRYPHNELAEIWKLMFENAAHDSIGGCNSDTTNRDVKHRYKLASDLAT 363 Query: 363 AIIEESLKAISGQIGTSGVACWGASGIPVTVFNTTGWDRTGTVSV 407 +++ +++ IS +I TVFN ++++G + + Sbjct: 364 NLLDLNMRLISEKIAQK-------QPFQFTVFNPLPYEKSGVIKM 401 >lin0449 [G] COG0383 Alpha-mannosidase Length = 860 Score = 140 bits (353), Expect = 6e-33, Method: Compositional matrix adjust. Identities = 117/427 (27%), Positives = 201/427 (47%), Gaps = 39/427 (9%) Query: 8 VLLAKLMNELLETLEKDEHYRYFHLDGQTIIIEDYLQIHPEKKEILERFIREGRIVIGPW 67 VL +L E+++ L+K F LDGQ I++DY+ ++PEK +++ I + ++ IGPW Sbjct: 25 VLSEQLFTEVIDELKKHPEAN-FVLDGQLSILDDYVALYPEKLADIKQLIADKQLFIGPW 83 Query: 68 YVLQDEFLTSSEANVRNLLTGHQDAAKYGVISKLGYFPDSFGNMGQAPQLLRQADIETAV 127 + D F E+ +RN + G D+ KYG K+GY PD+FG Q P LL A + + Sbjct: 84 FTQTDAFFAHGESILRNAMIGIFDSKKYGDYMKIGYLPDTFGFNAQMPTLLEHAGFDNVI 143 Query: 128 FGRGVKPTGFDNMVGELNSASYESPYSEMFWES-PDGSRVLGLLFANWYSNGNEVPAGPE 186 F RG+ +GE SPY + W+ + + + + Y G + E Sbjct: 144 FWRGIH-------LGE----HVASPYFK--WQGLGEEASIYAINMPQGYGTGMLL----E 186 Query: 187 EAKVFWDKKLADA----GKYASTPELLFMNGCDHQPVQRNLSDALETAKALYPDVDFVHS 242 + D +L A KY+ T E+L +G D + N ++ L + D+ S Sbjct: 187 PTSAYVDGRLDPAIDFIEKYSKTTEVLIPSGNDQLNIISNFAEKLAEINKMGRH-DYQLS 245 Query: 243 SFEQYLEALKPSLPANLVIVNGELRSQHTDGWGTLVNTASARIYLKQMNQRGQTLLEKGA 302 +++ +++ +K LP +L GE RS + ++ I LK + Q LL + Sbjct: 246 TYQDFIQYVK-ELP-DLEEYRGEFRSPVLARVHKTIGSSRMNIKLKSASLE-QKLLTR-I 301 Query: 303 EPLATLAYLASGQAYPHQLLNYAWKTLMQNHPHDSICGCSVDEVHREMTGRFEKSRHVGE 362 EPL +A ASG A +LL + WK L++ HDS+ GC D V ++ R +++ + + Sbjct: 302 EPLLVIAK-ASGIAISERLLMHTWKKLLEGQAHDSLAGCVSDPVAEDILHRMKEADELCD 360 Query: 363 AIIEESLKAISGQIGTSGVACWGASGIPVTVFNTTGWDRTGTVSVEVIAAKR--YFKEGP 420 +I +K I+ + + + +FNT D G ++V+ + +F P Sbjct: 361 SIENTIVKKIADDLSLAANE--------IIIFNTDLHDFDGYKEIQVVTEHKNIHFPAFP 412 Query: 421 NPAAIAE 427 + + E Sbjct: 413 DATIVQE 419 >BH0788 [G] COG0383 Alpha-mannosidase Length = 157 Score = 123 bits (308), Expect = 8e-28, Method: Compositional matrix adjust. Identities = 57/105 (54%), Positives = 79/105 (75%), Gaps = 1/105 (0%) Query: 1 MPYERHHVLLAKLMNELLETLEKDEHYRYFHLDGQTIIIEDYLQIHPEKKEILERFIREG 60 MP+E H L +LM++LLE D ++R FH+DGQTI++EDYL I PEK+ + ++I++G Sbjct: 19 MPFEGHRYYLIQLMDDLLELFATDPNFRSFHMDGQTIMLEDYLNIRPEKEAEVRKYIQDG 78 Query: 61 RIVIGPWYVLQDEFLTSSEANVRNLLTGHQDAAKYGVI-SKLGYF 104 R+VIGPWY+LQD FLTS+EANVRNLL G +D +G ++GYF Sbjct: 79 RLVIGPWYILQDAFLTSAEANVRNLLYGIKDTETFGQKREQIGYF 123 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.135 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 42,177,983 Number of extensions: 1802009 Number of successful extensions: 4366 Number of sequences better than 1.0e-05: 9 Number of HSP's gapped: 4352 Number of HSP's successfully gapped: 9 Length of query: 536 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 425 Effective length of database: 25,576,357 Effective search space: 10869951725 Effective search space used: 10869951725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 118 (50.1 bits)