BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0377.0016.p (251 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value alr1295 [O] COG0330 Membrane protease subunits, stomatin/prohibi... 87 4e-17 slr1106 [O] COG0330 Membrane protease subunits, stomatin/prohibi... 86 4e-17 SPCC1322.16 [O] COG0330 Membrane protease subunits, stomatin/pro... 82 7e-16 SPAC1782.06c [O] COG0330 Membrane protease subunits, stomatin/pr... 73 4e-13 YGR231c [O] COG0330 Membrane protease subunits, stomatin/prohibi... 68 2e-11 >alr1295 [O] COG0330 Membrane protease subunits, stomatin/prohibitin homologs Length = 359 Score = 86.7 bits (213), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 8/166 (4%), Positives = 29/166 (17%), Gaps = 2/166 (1%) Query: 1 VIIGGNAFTSVEYGHVGLYKTFGKLNNNMLSPGIHFKIPFIQTVIQVNTQVTKAETDTSA 60 + + N + ++ +A + Sbjct: 10 RRALRHNLQRLRELAPASKMVAVVKANAYGHGLLETARTLPDADAFGVARLEEALRLRAG 69 Query: 61 SSKDLQPVSTHVAVNYSVNKDSAYNLMNNIGG--NFDGVIINPAIQEIVKEVTAKYPAED 118 + + SA + + + + + + Sbjct: 70 GITKPVLLLEGFFDARDLPTISAQHFHTAVHNEEQLAALEEASLDEPVTVWMKLDTGMHR 129 Query: 119 LITRRDLVAGEISELLTKRLGRYDLVVNDINIVNFKFSDAFNQSIE 164 L R + L + R + + + + Sbjct: 130 LGVRPEQAEAFYHRLTQCKNVRQPVNIVSHFARADEPKCGATEKQL 175 >slr1106 [O] COG0330 Membrane protease subunits, stomatin/prohibitin homologs Length = 282 Score = 86.3 bits (212), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 50/166 (30%), Positives = 81/166 (48%), Gaps = 2/166 (1%) Query: 1 VIIGGNAFTSVEYGHVGLYKTFGKLNNNMLSPGIHFKIPFIQTVIQVNTQVTKAETDTSA 60 V++ N+F + G G+ GK + L GIHFK P + +V + V K E + Sbjct: 23 VLLSFNSFVVINPGQAGVLSVLGKAQDGALLEGIHFKPPLVSSVDIYDVTVQKFEVPAQS 82 Query: 61 SSKDLQPVSTHVAVNYSVNKDSAYNLMNNIGG--NFDGVIINPAIQEIVKEVTAKYPAED 118 S+KDLQ +S A+N+ ++ + G N II P QE K A+ E+ Sbjct: 83 STKDLQDLSASFAINFRLDPTEVVTIRRTQGTLQNIVAKIIAPQTQESFKIAAARRTVEE 142 Query: 119 LITRRDLVAGEISELLTKRLGRYDLVVNDINIVNFKFSDAFNQSIE 164 IT+R + + L RL +Y ++V D ++V+ FS F +++E Sbjct: 143 AITKRSELKEDFDNALNSRLEKYGIIVLDTSVVDLAFSPEFAKAVE 188 >SPCC1322.16 [O] COG0330 Membrane protease subunits, stomatin/prohibitin homologs Length = 136 Score = 82.0 bits (201), Expect = 7e-16, Method: Compositional matrix adjust. Identities = 0/156 (0%), Positives = 0/156 (0%), Gaps = 1/156 (0%) Query: 10 SVEYGHVGL-YKTFGKLNNNMLSPGIHFKIPFIQTVIQVNTQVTKAETDTSASSKDLQPV 68 + G + + + + + AE A + Sbjct: 38 APAPIDAGQAFGEGVEGRSAGIDGDVLLLAVEGHQGGLRIAVGRHAEGHHLAVGAEALKA 97 Query: 69 STHVAVNYSVNKDSAYNLMNNIGGNFDGVIINPAIQEIVKEVTAKYPAEDLITRRDLVAG 128 Sbjct: 98 157 Query: 129 EISELLTKRLGRYDLVVNDINIVNFKFSDAFNQSIE 164 Sbjct: 158 193 >SPAC1782.06c [O] COG0330 Membrane protease subunits, stomatin/prohibitin homologs Length = 282 Score = 73.2 bits (178), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 39/147 (26%), Positives = 76/147 (51%) Query: 18 LYKTFGKLNNNMLSPGIHFKIPFIQTVIQVNTQVTKAETDTSASSKDLQPVSTHVAVNYS 77 L+ + ++ G HF IP++Q I + + T+ SKDLQ VS + V + Sbjct: 37 LFDRLSGVQKQVVQEGTHFLIPWLQKAIVYDVRTRPRNIATTTGSKDLQMVSLTLRVLHR 96 Query: 78 VNKDSAYNLMNNIGGNFDGVIINPAIQEIVKEVTAKYPAEDLITRRDLVAGEISELLTKR 137 + N+G ++D ++ EI+K V A++ A +LIT+R++V+ +I + L +R Sbjct: 97 PEVGMLPQIYQNLGLDYDERVLPSIGNEILKSVVAQFDAAELITQREVVSAKIRQELVQR 156 Query: 138 LGRYDLVVNDINIVNFKFSDAFNQSIE 164 + + + D++I + F F +++E Sbjct: 157 ATEFGIRLEDVSITHMTFGKEFTKAVE 183 >YGR231c [O] COG0330 Membrane protease subunits, stomatin/prohibitin homologs Length = 315 Score = 67.8 bits (164), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 40/160 (25%), Positives = 82/160 (51%), Gaps = 1/160 (0%) Query: 6 NAFTSVEYGHVGL-YKTFGKLNNNMLSPGIHFKIPFIQTVIQVNTQVTKAETDTSASSKD 64 NA +V+ GH + Y +++ + + G HF P++ T I + + + +KD Sbjct: 56 NALFNVDGGHRAIVYSRIHGVSSRIFNEGTHFIFPWLDTPIIYDVRAKPRNVASLTGTKD 115 Query: 65 LQPVSTHVAVNYSVNKDSAYNLMNNIGGNFDGVIINPAIQEIVKEVTAKYPAEDLITRRD 124 LQ V+ V + + +G ++D ++ + E++K V A++ A LIT+R+ Sbjct: 116 LQMVNITCRVLSRPDVVQLPTIYRTLGQDYDERVLPSIVNEVLKAVVAQFNASQLITQRE 175 Query: 125 LVAGEISELLTKRLGRYDLVVNDINIVNFKFSDAFNQSIE 164 V+ I E L +R +++++++D++I FS F ++E Sbjct: 176 KVSRLIRENLVRRASKFNILLDDVSITYMTFSPEFTNAVE 215 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.133 0.366 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,492,479 Number of extensions: 561530 Number of successful extensions: 906 Number of sequences better than 1.0e-05: 9 Number of HSP's gapped: 907 Number of HSP's successfully gapped: 9 Length of query: 251 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 147 Effective length of database: 26,383,695 Effective search space: 3878403165 Effective search space used: 3878403165 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 114 (48.5 bits)