BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0377.0007.p (427 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1231 [P] COG1055 Na+/H+ antiporter NhaD and related arsenite p... 310 3e-84 Rv2684 [P] COG1055 Na+/H+ antiporter NhaD and related arsenite p... 273 5e-73 MT2758 [P] COG1055 Na+/H+ antiporter NhaD and related arsenite p... 273 6e-73 TM0934 [P] COG1055 Na+/H+ antiporter NhaD and related arsenite p... 270 4e-72 Rv2685 [P] COG1055 Na+/H+ antiporter NhaD and related arsenite p... 263 3e-70 >BH1231 [P] COG1055 Na+/H+ antiporter NhaD and related arsenite permeases Length = 428 Score = 310 bits (794), Expect = 3e-84, Method: Compositional matrix adjust. Identities = 168/421 (39%), Positives = 257/421 (61%), Gaps = 1/421 (0%) Query: 8 AIGIFLLTYGLIISEKVHRTVXXXXXXXXXXXXXXVSQETA-LEHIDFNTXXXXXXXXXX 66 A+ IF++ Y LI++E +R V + A L++ID++T Sbjct: 6 ALVIFVICYLLIMAETFNRAVVACLGGVLMLFVGIYPLDAAFLQYIDWHTITLLLSMMIL 65 Query: 67 XXITAETGLFSYVAVKAAKLAKGEPKRIXXXXXXXXXXXXXFLDNVTTVLLMVPVTFSIT 126 IT+++G F Y+A++ A+ G P + FL+NVT VLL+VP+ ++T Sbjct: 66 VSITSQSGFFEYMAIRLAQKVDGRPIPLLIVVAGITAIGSAFLNNVTMVLLIVPIVLTLT 125 Query: 127 RQLRIHPLPFLLSQIIASNVGGTATLIGDPPNIMIGSAVKELTFMSFISNLTPVILIIIL 186 L+++ +PFLL+ ++ASN+GGTATLIGDPPN+MIG AV L+F F+ +L P++ +I Sbjct: 126 NLLKLNAVPFLLTLVMASNIGGTATLIGDPPNLMIGQAVAHLSFNDFLLHLGPIVAVIFF 185 Query: 187 AYLPLFLLMFGKQLKTSPELQRSVMEMDERAMITDRKLLRRCLWVLGLTILGFFLHQAIH 246 L +L + +QL + E + + +D + + +R LL + + VL LT +GF + + Sbjct: 186 VVLIGLILYYRRQLSVTAEDRSRLRGVDPTSYLKNRPLLIKSMTVLTLTTIGFTIQPFLP 245 Query: 247 LESATVXXXXXXXXXXXXXXEKMLHKAFASVEWVTIFFFIGLFVLVSGLVETGVIAELAG 306 +E ++ E+ + F SVEWVT+FFFIGLF+LV G+ E G+I E+A Sbjct: 246 VELTSIAMAGALLLMLITQNEQDMEGVFRSVEWVTLFFFIGLFMLVGGIKEVGIIDEIAK 305 Query: 307 QAMELTGGNLAASSMLILWLSAIASAFLDNIPFVATMIPLIQEMGNLGIHNLEPLWWSLA 366 + T G++ +S+ ILW S I S F+DNIPFVA MIP+I E G+ L+PLWWSLA Sbjct: 306 AIIYYTEGDIPKTSLFILWGSGILSGFVDNIPFVAAMIPVILEFQEYGVGELDPLWWSLA 365 Query: 367 LGACLGGNGTLIGASANLIVAGLAAKEGYPITFMRYLKVGFPLMLLSMVISSLYIYLRYL 426 LGACLGGN T+IGA+AN+IVAGLA + +++ +LKVG P+ L+S ++ ++Y+Y+RYL Sbjct: 366 LGACLGGNATVIGATANVIVAGLAVQAKQSFSYLEFLKVGAPVALVSFLLCTVYLYIRYL 425 Query: 427 I 427 I Sbjct: 426 I 426 >Rv2684 [P] COG1055 Na+/H+ antiporter NhaD and related arsenite permeases Length = 429 Score = 273 bits (697), Expect = 5e-73, Method: Compositional matrix adjust. Identities = 158/426 (37%), Positives = 243/426 (57%), Gaps = 10/426 (2%) Query: 8 AIGIFLLTYGLIISEKVHRT-VXXXXXXXXXXXXXXVSQETALEH---IDFNTXXXXXXX 63 A+ IF+ Y LI S++V++T V S + H ID++ Sbjct: 5 AVTIFVAAYVLIASDRVNKTMVALTGAAAVVVLPVITSHDIFYSHDTGIDWDVIFLLVGM 64 Query: 64 XXXXXITAETGLFSYVAVKAAKLAKGEPKRIXXXXXXXXXXXXXFLDNVTTVLLMVPVTF 123 + +TG+F Y A+ AAK A+G P RI LDNVTTVLL+ PVT Sbjct: 65 MIIVGVLRQTGVFEYTAIWAAKRARGSPLRIMILLVLVSALASALLDNVTTVLLIAPVTL 124 Query: 124 SITRQLRIHPLPFLLSQIIASNVGGTATLIGDPPNIMIGSAVKELTFMSFISNLTPVILI 183 + +L I+ FL++++ ASN+GG ATL+GDPPNI++ S LTF F+ +LTP+++I Sbjct: 125 LVCDRLNINTTSFLMAEVFASNIGGAATLVGDPPNIIVASR-AGLTFNDFMLHLTPLVVI 183 Query: 184 IILAYLPLFLLMFGKQLKTSPELQRSVMEMDERAMITDRKLLRRCLWVLGLTILGFFLHQ 243 +++A + + +FG + + VM +DE I DR LL +C VL L F H Sbjct: 184 VLIALIAVLPRLFG-SITVEADRIADVMALDEGEAIRDRGLLVKCGAVLVLVFAAFVAHP 242 Query: 244 AIHLESATVXXXXXXXXXXXXXXEKMLHKAFASVEWVTIFFFIGLFVLVSGLVETGVIAE 303 +H++ + V + + +SVEW T+ FF GLF++V LV+TGV+ + Sbjct: 243 VLHIQPSLVALLGAGMLIVVSGLTR--SEYLSSVEWDTLLFFAGLFIMVGALVKTGVVND 300 Query: 304 LAGQAMELTGGNLAASSMLILWLSAIASAFLDNIPFVATMIPLIQEMGNL--GIHNLEPL 361 LA A +LTGGN+ A++ LIL +SA S +DNIP+VATM PL+ E+ + G + + Sbjct: 301 LARAATQLTGGNIVATAFLILGVSAPISGIIDNIPYVATMTPLVAELVAVMGGQPSTDTP 360 Query: 362 WWSLALGACLGGNGTLIGASANLIVAGLAAKEGYPITFMRYLKVGFPLMLLSMVISSLYI 421 WW+LALGA GGN T IGASAN+++ G+A + G PI+F + + G + +S+ ++++Y+ Sbjct: 361 WWALALGADFGGNLTAIGASANVVMLGIARRAGAPISFWEFTRKGAVVTAVSIALAAIYL 420 Query: 422 YLRYLI 427 +LRY + Sbjct: 421 WLRYFV 426 >MT2758 [P] COG1055 Na+/H+ antiporter NhaD and related arsenite permeases Length = 429 Score = 273 bits (697), Expect = 6e-73, Method: Compositional matrix adjust. Identities = 158/426 (37%), Positives = 243/426 (57%), Gaps = 10/426 (2%) Query: 8 AIGIFLLTYGLIISEKVHRT-VXXXXXXXXXXXXXXVSQETALEH---IDFNTXXXXXXX 63 A+ IF+ Y LI S++V++T V S + H ID++ Sbjct: 5 AVTIFVAAYVLIASDRVNKTMVALTGAAAVVVLPVITSHDIFYSHDTGIDWDVIFLLVGM 64 Query: 64 XXXXXITAETGLFSYVAVKAAKLAKGEPKRIXXXXXXXXXXXXXFLDNVTTVLLMVPVTF 123 + +TG+F Y A+ AAK A+G P RI LDNVTTVLL+ PVT Sbjct: 65 MIIVGVLRQTGVFEYTAIWAAKRARGSPLRIMILLVLVSALASALLDNVTTVLLIAPVTL 124 Query: 124 SITRQLRIHPLPFLLSQIIASNVGGTATLIGDPPNIMIGSAVKELTFMSFISNLTPVILI 183 + +L I+ FL++++ ASN+GG ATL+GDPPNI++ S LTF F+ +LTP+++I Sbjct: 125 LVCDRLNINTTSFLMAEVFASNIGGAATLVGDPPNIIVASR-AGLTFNDFMLHLTPLVVI 183 Query: 184 IILAYLPLFLLMFGKQLKTSPELQRSVMEMDERAMITDRKLLRRCLWVLGLTILGFFLHQ 243 +++A + + +FG + + VM +DE I DR LL +C VL L F H Sbjct: 184 VLIALIAVLPRLFG-SITVEADRIADVMALDEGEAIRDRGLLVKCGAVLVLVFAAFVAHP 242 Query: 244 AIHLESATVXXXXXXXXXXXXXXEKMLHKAFASVEWVTIFFFIGLFVLVSGLVETGVIAE 303 +H++ + V + + +SVEW T+ FF GLF++V LV+TGV+ + Sbjct: 243 VLHIQPSLVALLGAGMLIAVSGLTR--SEYLSSVEWDTLLFFAGLFIMVGALVKTGVVND 300 Query: 304 LAGQAMELTGGNLAASSMLILWLSAIASAFLDNIPFVATMIPLIQEMGNL--GIHNLEPL 361 LA A +LTGGN+ A++ LIL +SA S +DNIP+VATM PL+ E+ + G + + Sbjct: 301 LARAATQLTGGNIVATAFLILGVSAPISGIIDNIPYVATMTPLVAELVAVMGGQPSTDTP 360 Query: 362 WWSLALGACLGGNGTLIGASANLIVAGLAAKEGYPITFMRYLKVGFPLMLLSMVISSLYI 421 WW+LALGA GGN T IGASAN+++ G+A + G PI+F + + G + +S+ ++++Y+ Sbjct: 361 WWALALGADFGGNLTAIGASANVVMLGIARRAGAPISFWEFTRKGAVVTAVSIALAAIYL 420 Query: 422 YLRYLI 427 +LRY + Sbjct: 421 WLRYFV 426 >TM0934 [P] COG1055 Na+/H+ antiporter NhaD and related arsenite permeases Length = 425 Score = 270 bits (689), Expect = 4e-72, Method: Compositional matrix adjust. Identities = 155/429 (36%), Positives = 242/429 (56%), Gaps = 8/429 (1%) Query: 1 MEQQGIWAIGIFLLTYGLIISEKVHRTVXXXXXXXXXXXXXXVSQ-ETALEHIDFNTXXX 59 M + + ++ IF++ Y II EK HR V E + ++DFNT Sbjct: 1 MFEDAVVSLLIFIIVYLFIILEKHHRAVITMLGGSAALFLVFKDPIEALVRYVDFNTIFL 60 Query: 60 XXXXXXXXXITAETGLFSYVAVKAAKLAKGEPKRIXXXXXXXXXXXXXFLDNVTTVLLMV 119 +T +GLF ++ + + KL+ G FLDNVTT+L+ V Sbjct: 61 LIGMMIFVSVTKRSGLFHFLGLYSVKLSGGNVFLFFIAINTLVALLSSFLDNVTTILVFV 120 Query: 120 PVTFSITRQLRIHPLPFLLSQIIASNVGGTATLIGDPPNIMIGSAVKELTFMSFISNLTP 179 PVT + + + P+PF++S+II+SN+GGTAT+IGDPPNIMI SA K L F+ F+ N+ P Sbjct: 121 PVTLVVCDTVDLDPVPFVISEIISSNIGGTATMIGDPPNIMIASAAK-LHFLDFVVNVAP 179 Query: 180 VILIIILAYLPLFLLMFGKQLKTSPELQRSVMEMDERAMITDRKLLRRCLWVLGLTILGF 239 ++ ++ L +FL +++ V + D R I D+KL + + + ++ F Sbjct: 180 AAVLTLIVTL-IFLSAVYRRVIFRKIPVEVVKDFDPRRAIVDKKLFYLSIILTLVVLVLF 238 Query: 240 FLHQAIHLESATVXXXXXXXXXXXXXXEKMLHKAFASVEWVTIFFFIGLFVLVSGLVETG 299 L + + LES V +K + F +EW IFFFIGLF++V L E G Sbjct: 239 SLQKPLGLESFEV-ALLAGFLSLAFLKKKDIEDVFKEIEWGVIFFFIGLFLVVGALEEAG 297 Query: 300 VIAELAGQAMELTGGNLAASSMLILWLSAIASAFLDNIPFVATMIPLIQEMGNLG---IH 356 V+ ++ + L+ G + ++ + +L +S ++SAF+DNIPF ATMIP+I+++ L Sbjct: 298 VLERISELVIRLSKGKMESTLISVLGISGLSSAFVDNIPFTATMIPVIKKLAVLAPETFS 357 Query: 357 NLEPLWWSLALGACLGGNGTLIGASANLIVAGLAAKEGYPITFMRYLKVGFPLMLLSMVI 416 +L PLWW+L+LGAC GGNGTLIGASAN++ L A + ITF Y K+GFP+++LS+++ Sbjct: 358 DLRPLWWALSLGACFGGNGTLIGASANVVGTSLIADRKH-ITFWEYFKIGFPVLILSLLV 416 Query: 417 SSLYIYLRY 425 S +Y+ +RY Sbjct: 417 SGVYLLIRY 425 >Rv2685 [P] COG1055 Na+/H+ antiporter NhaD and related arsenite permeases Length = 428 Score = 263 bits (673), Expect = 3e-70, Method: Compositional matrix adjust. Identities = 157/426 (36%), Positives = 235/426 (55%), Gaps = 10/426 (2%) Query: 8 AIGIFLLTYGLIISEKVHRTVXXXXXXXXXXXXXXV-SQETALEH---IDFNTXXXXXXX 63 AI +F+ Y LI S++V +T V S + H ID++ Sbjct: 5 AITVFVAGYALIASDRVSKTRVALTCAAIMVGAGIVGSDDVFYSHEAGIDWDVIFLLLGM 64 Query: 64 XXXXXITAETGLFSYVAVKAAKLAKGEPKRIXXXXXXXXXXXXXFLDNVTTVLLMVPVTF 123 + TG+F YVA+ A K A P RI LDNVTTVLL+ PVT Sbjct: 65 MIIVSVLRHTGVFEYVAIWAVKRANAAPLRIMILLVLVTALGSALLDNVTTVLLIAPVTL 124 Query: 124 SITRQLRIHPLPFLLSQIIASNVGGTATLIGDPPNIMIGSAVKELTFMSFISNLTPVILI 183 + +L ++ PFL++++ ASNVGG ATL+GDPPNI+I S LTF F+ ++ P +L+ Sbjct: 125 LVCDRLGVNSTPFLVAEVFASNVGGAATLVGDPPNIIIASR-AGLTFNDFLIHMAPAVLV 183 Query: 184 IILAYLPLFLLMFGKQLKTSPELQRSVMEMDERAMITDRKLLRRCLWVLGLTILGFFLHQ 243 +++A + L + G + P+ V+ ++ER I DR LL +C VL L F H Sbjct: 184 VMIALIGLLPWLLG-SVTAEPDRVADVLSLNEREAIHDRGLLIKCGVVLVLVFAAFIAHP 242 Query: 244 AIHLESATVXXXXXXXXXXXXXXEKMLHKAFASVEWVTIFFFIGLFVLVSGLVETGVIAE 303 +H++ + V E+ +SVEW T+ FF GLFV+V LV+TGV+ + Sbjct: 243 VLHIQPSLVALLGAGVLVRFSGLER--SDYLSSVEWDTLLFFAGLFVMVGALVKTGVVEQ 300 Query: 304 LAGQAMELTGGNLAASSMLILWLSAIASAFLDNIPFVATMIPLIQEMGNLGIHNLEP--L 361 LA A ELTGGN + LIL +SA S +DNIP+VATM P++ E+ ++ P Sbjct: 301 LARAATELTGGNELLTVGLILGISAPVSGIIDNIPYVATMTPIVTELVAAMPGHVHPDTF 360 Query: 362 WWSLALGACLGGNGTLIGASANLIVAGLAAKEGYPITFMRYLKVGFPLMLLSMVISSLYI 421 WW+LAL A GGN T + ASAN+++ G+A + G PI+F ++ + G + +S+V+S++Y+ Sbjct: 361 WWALALSADFGGNLTAVAASANVVMLGIARRSGTPISFWKFTRKGAVVTAVSLVLSAVYL 420 Query: 422 YLRYLI 427 +LRY + Sbjct: 421 WLRYFV 426 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.327 0.141 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 25,194,059 Number of extensions: 894062 Number of successful extensions: 2964 Number of sequences better than 1.0e-05: 34 Number of HSP's gapped: 2886 Number of HSP's successfully gapped: 37 Length of query: 427 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 318 Effective length of database: 25,807,025 Effective search space: 8206633950 Effective search space used: 8206633950 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 117 (49.7 bits)