BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0373.0020.p (475 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin0220 [G] COG1653 ABC-type sugar transport system, periplasmic... 94 4e-19 PH0753 [G] COG1653 ABC-type sugar transport system, periplasmic ... 87 6e-17 Rv2041c [G] COG1653 ABC-type sugar transport system, periplasmic... 80 9e-15 MT2101 [G] COG1653 ABC-type sugar transport system, periplasmic ... 80 9e-15 TM1204 [G] COG2182 Maltose-binding periplasmic proteins/domains 78 4e-14 >lin0220 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 418 Score = 94.4 bits (233), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 84/303 (27%), Positives = 142/303 (46%), Gaps = 21/303 (6%) Query: 100 LDDLIAEFHRLHPGI--HVLPLALNNFDHVNSAMELINNGMLDVLSLNSTQFQELAENGS 157 LD ++ EF + +P I +V +A N++ ++ D LN F + AE G Sbjct: 47 LDKMVKEFEKQNPDIKVNVQTIAFNDY-FTKLQTQIAGGDAPDAFELNYETFMQYAEKGV 105 Query: 158 -AGLLETLEPDPGLYPI-----ASEAFLHNGALFAYPISFSPVVLCYNKAHFREADLPEP 211 A L +E D P A +AF ++G + SFS VV YNK F +A + P Sbjct: 106 LADLTSYIEKDKDFDPSTLNKQAYDAFKYDGKQYGMVESFSNVVTIYNKDLFDKAGVEYP 165 Query: 212 DSYWTWDDLIEAARKLSEKRGRHAVYFVPASENRYSVFLLQSGIRTMEDGEHHQTL-SPE 270 + WTW D AA+KL++ + + P + N + Q+G + T+ SPE Sbjct: 166 TADWTWKDEEAAAKKLTDAKNKVWGTSQPVTMNEFFKVAAQNGGSIFDKDMTKTTINSPE 225 Query: 271 TANSLNVYSRLVNNHQIF--PKYLAGNHDDETVSLFVQEQVSMILTTYYNLNDFKDLSLD 328 +L + V ++ P L+G ++ LF+ Q++M+ T + + F+D Sbjct: 226 NVEALTHLTNEVTESKVAPSPSDLSGQLPED---LFMNGQIAMLHTGIWLFDMFQDAPFK 282 Query: 329 YDLAPLPTLKAKDPQK-TLLVTIGAAVVENSRQKEAARQFVSFL-ASPEAQRMIRERSVS 386 +D+ ++A + QK T G V ++S +KEAA +F SF+ A+ EA ++ + Sbjct: 283 WDV----QVEAGNKQKATHFFANGIGVSKDSDKKEAAFKFASFMSANKEAAKIRIANNWE 338 Query: 387 IPA 389 +PA Sbjct: 339 LPA 341 >PH0753 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 464 Score = 87.0 bits (214), Expect = 6e-17, Method: Compositional matrix adjust. Identities = 80/337 (23%), Positives = 145/337 (43%), Gaps = 31/337 (9%) Query: 80 VKERIRIHF-GCSSSLTKDFKLDDLIAEFHRLHPGIHVLPLALNNFDHVNSAMELINNGM 138 ++ ++ I F G S+ T+ +I F + +P I V + H N Sbjct: 64 IQNQVFIRFAGWSAGETEMKNYQRMIELFEKENPNIKVKYEVITQMFHENILASFGAGVA 123 Query: 139 LDVLSLNSTQFQELAENGSAGLLETLEPDP---GLYPIASEAFLHNGALFAYPISFSPVV 195 DV ++S+ E G+ + L + YP E F NG L+ P +S + Sbjct: 124 PDVFYVDSSWAPIFIEKGALYPISELADESFINKFYPFLLEPFKKNGKLYGLPKDWSMLA 183 Query: 196 LCYNKAHFREADLPEPDSYWTWDDLIEAARKLSEKRGRHAVYFVPASENRYSVFLLQSG- 254 L YNK F +A L +P TWD+L+E A+ ++EK G+ + NRY + +G Sbjct: 184 LFYNKKLFEQAGLTKPPE--TWDELLEYAKIITEKTGKPGLAIYLGGFNRYVPVAVSNGA 241 Query: 255 ----IRTMEDGEHHQTLSPETANSLNVYSRLVNNHQI------FPKYLAGNHDDETVSL- 303 ED +P +L Y L ++ P Y+ D L Sbjct: 242 PKPWFEKPEDASWFD--NPVVRETLTWYINLYRKGKVEREKKGLPPYVVAPEDVGAGWLG 299 Query: 304 --FVQEQVSMILTTYYNL----NDFKDLSL--DYDLAPLPTLKAKDPQKTLLVTIGAAVV 355 F Q++V+M+++ + + + F + D+D+APLP K+ + T++ T+ + Sbjct: 300 DAFGQQKVAMVISGNWMIPFLADQFPNFKYGEDWDIAPLPA--GKNGRVTMVYTVILGIN 357 Query: 356 ENSRQKEAARQFVSFLASPEAQR-MIRERSVSIPARK 391 ++ + A +FV F+ P Q+ ++ + ++P+ K Sbjct: 358 SKTKHPKEAWKFVEFILGPRGQKELVVKAGQTLPSMK 394 >Rv2041c [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 439 Score = 79.7 bits (195), Expect = 9e-15, Method: Compositional matrix adjust. Identities = 82/317 (25%), Positives = 131/317 (41%), Gaps = 41/317 (12%) Query: 103 LIAEFHRLHPGIHVLPLALNNFDHVNSAMELINNGMLDVLSLNSTQFQELAENGSAGLLE 162 ++ EF R +P I V L A DVL + ELA+ G L Sbjct: 55 VVNEFQRRYPDIKVRALLSGPGVMQQLATFCAGGKCPDVLMAWELTYAELADRGVLLDLN 114 Query: 163 TL-------------EPDPGLYPIASEAFLHNGALFAYPISFSPVVLCYNKAHFREADLP 209 TL + LY E F NG +A+P +S L YNK F +A +P Sbjct: 115 TLLARDQAFAAELKSDSIGALY----ETFTFNGGQYAFPEQWSGNFLFYNKQLFDDAGVP 170 Query: 210 EPDSY----WTWDDLIEAARKLSE--KRGRHAVY-FVPASENRYSVFLLQSGIRTMEDGE 262 P W++ + ++AA+ L++ + GR + FV A + Y+ +G+ M +G Sbjct: 171 PPPGSWERPWSFAEFLDAAQALTKQGRSGRDRQWGFVNAWVSFYA-----AGLFAMNNGV 225 Query: 263 HHQT--LSPETAN--------SLNVYSRLVNNHQIFPKYLAGNHDDETVSLFVQEQVSMI 312 ++P N ++ Y+ L N H++ P A T LF + + Sbjct: 226 PWSVPRMNPTHLNFDHDGFLEAVQFYADLTNKHKVAPS-AAEQQSMSTADLFSVGKAGIA 284 Query: 313 LTTYYNLNDF-KDLSLDYDLAPLPTLKAKDPQKTLLVTIGAAVVENSRQKEAARQFVSFL 371 L ++ F + LD+D+APLP + + G A+ SR+K+ A +FV F Sbjct: 285 LAGHWRYQTFDRADGLDFDVAPLPIGPRGRAACSDIGVTGLAIAATSRRKDQAWEFVKFA 344 Query: 372 ASPEAQRMIRERSVSIP 388 P Q +I E + +P Sbjct: 345 TGPVGQALIGESRLFVP 361 >MT2101 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 439 Score = 79.7 bits (195), Expect = 9e-15, Method: Compositional matrix adjust. Identities = 82/317 (25%), Positives = 131/317 (41%), Gaps = 41/317 (12%) Query: 103 LIAEFHRLHPGIHVLPLALNNFDHVNSAMELINNGMLDVLSLNSTQFQELAENGSAGLLE 162 ++ EF R +P I V L A DVL + ELA+ G L Sbjct: 55 VVNEFQRRYPDIKVRALLSGPGVMQQLATFCAGGKCPDVLMAWELTYAELADRGVLLDLN 114 Query: 163 TL-------------EPDPGLYPIASEAFLHNGALFAYPISFSPVVLCYNKAHFREADLP 209 TL + LY E F NG +A+P +S L YNK F +A +P Sbjct: 115 TLLARDQAFAAELKSDSIGALY----ETFTFNGGQYAFPEQWSGNFLFYNKQLFDDAGVP 170 Query: 210 EPDSY----WTWDDLIEAARKLSE--KRGRHAVY-FVPASENRYSVFLLQSGIRTMEDGE 262 P W++ + ++AA+ L++ + GR + FV A + Y+ +G+ M +G Sbjct: 171 PPPGSWERPWSFAEFLDAAQALTKQGRSGRDRQWGFVNAWVSFYA-----AGLFAMNNGV 225 Query: 263 HHQT--LSPETAN--------SLNVYSRLVNNHQIFPKYLAGNHDDETVSLFVQEQVSMI 312 ++P N ++ Y+ L N H++ P A T LF + + Sbjct: 226 PWSVPRMNPTHLNFDHDGFLEAVQFYADLTNKHKVAPS-AAEQQSMSTADLFSVGKAGIA 284 Query: 313 LTTYYNLNDF-KDLSLDYDLAPLPTLKAKDPQKTLLVTIGAAVVENSRQKEAARQFVSFL 371 L ++ F + LD+D+APLP + + G A+ SR+K+ A +FV F Sbjct: 285 LAGHWRYQTFDRADGLDFDVAPLPIGPRGRAACSDIGVTGLAIAATSRRKDQAWEFVKFA 344 Query: 372 ASPEAQRMIRERSVSIP 388 P Q +I E + +P Sbjct: 345 TGPVGQALIGESRLFVP 361 >TM1204 [G] COG2182 Maltose-binding periplasmic proteins/domains Length = 391 Score = 77.8 bits (190), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 74/267 (27%), Positives = 120/267 (44%), Gaps = 36/267 (13%) Query: 151 ELAENGSAGLLETLEPDPGL------YPIASEAFLHNGALFAYPISFSPVVLCYNKAHFR 204 ELA NG +EP P Y A AF + G L+ P + + L YNK + Sbjct: 85 ELAVNG------LIEPIPNFSDLKNFYETALNAFSYGGKLYGIPYAMEAIALIYNKDY-- 136 Query: 205 EADLPEPDSYWTWDDLIEAARKLSEKRGRHAVYFVPASENRYSV--FLLQSGIRTMEDGE 262 +PEP T D+LIE A+++ E+ G F+ ++ Y + F+ G + E Sbjct: 137 ---VPEPPK--TMDELIEIAKQIDEEFGGEVRGFITSAAEFYYIAPFIFGYGGYVFKQTE 191 Query: 263 -----HHQTLSPETA-NSLNVYSRLVNNHQIFPKYLAGNHDDETV--SLFVQEQVSMILT 314 + L+ E A + + RLV+ + P D+ + S+F + Q +MI+ Sbjct: 192 KGLDVNDIGLANEGAIKGVKLLKRLVDEGILDP------SDNYQIMDSMFREGQAAMIIN 245 Query: 315 TYYNLNDFKDLSLDYDLAPLPTLKAKDPQKTLLVTIGAAVVENSRQKEAARQFV-SFLAS 373 + + +KD +DY +AP+P L+ P + + G V S K A +F+ SF+A Sbjct: 246 GPWAIKAYKDAGIDYGVAPIPDLEPGVPARPFVGVQGFMVNAKSPNKLLAIEFLTSFIAK 305 Query: 374 PEAQRMIRERSVSIPARKRNAEMVVDD 400 E I +P+RK E+V D+ Sbjct: 306 KETMYRIYLGDPRLPSRKDVLELVKDN 332 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.135 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 32,939,252 Number of extensions: 1348184 Number of successful extensions: 3575 Number of sequences better than 1.0e-05: 16 Number of HSP's gapped: 3577 Number of HSP's successfully gapped: 16 Length of query: 475 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 365 Effective length of database: 25,691,691 Effective search space: 9377467215 Effective search space used: 9377467215 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 118 (50.1 bits)