BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0373.0014.p (363 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp1011_1 [ER] COG1063 Threonine dehydrogenase and related Zn-de... 166 5e-41 SMa1417 [ER] COG1063 Threonine dehydrogenase and related Zn-depe... 83 6e-16 YPO3352 [ER] COG1063 Threonine dehydrogenase and related Zn-depe... 80 5e-15 SA0239 [ER] COG1063 Threonine dehydrogenase and related Zn-depen... 79 8e-15 BH0189 [ER] COG1063 Threonine dehydrogenase and related Zn-depen... 78 2e-14 >RSp1011_1 [ER] COG1063 Threonine dehydrogenase and related Zn-dependent dehydrogenases Length = 397 Score = 166 bits (420), Expect = 5e-41, Method: Compositional matrix adjust. Identities = 99/289 (34%), Positives = 150/289 (51%), Gaps = 16/289 (5%) Query: 60 KPVVLGYSAAGVIRSRGKGMEHWPEGQRVACYGAPYAKHAEWLLMPQHLMVPVPEHVSPE 119 +P+ LGY G + + G G+E + G RV G+ HA+ + +P++L +P+ V E Sbjct: 89 QPIPLGYCNVGTVHAVGAGVEGFKVGDRVVSNGS----HADVVRVPRNLCARIPDEVDDE 144 Query: 120 EASTVGLGAIAVHAVRQADLQFGETLVLIGAGILGQLIAQIARAAGCRVIVYDLLAERCR 179 A + AI + +R A+ GE V+ G G++G L Q+ RA GCRV+ D +E+ + Sbjct: 145 SAVFTVVAAIGLQGIRLAEPTLGEAFVVTGVGLIGLLAVQLLRAHGCRVLAIDFDSEKLK 204 Query: 180 TAESLGIRHIAVNPQRVSRHLDNLTAGM------GADAVIICAGGKSGGLVDQGLEWVRN 233 A G NP D + AG+ G D VII A S V Q + R Sbjct: 205 LAAQFGAE--TCNP---GLREDPVAAGLAFSRGRGVDGVIITASTASSEPVTQAAQMCRK 259 Query: 234 QGKVLLVGDVKPDFSRELMFGKEAQVLISRAGGPGRYDPVYEKQGIDYPYGYVRWTEGRN 293 +G+++LVG + +R + KE +S + GPGRYDP YE+QG DYP G+VRWTE RN Sbjct: 260 RGRIVLVGVTGLELNRADFYEKELSFQVSCSYGPGRYDPTYEEQGRDYPLGFVRWTEQRN 319 Query: 294 MAEYIRLISEGDIRVKPLISSVFPLERSADAFRQYAESPAELLGAVLAY 342 + ++++ + V+PLI+ P + + A+ S LG VL Y Sbjct: 320 FEAVLDMLADRRLDVRPLITHRIPFDEAPRAY-DLLSSDKSALGIVLEY 367 >SMa1417 [ER] COG1063 Threonine dehydrogenase and related Zn-dependent dehydrogenases Length = 341 Score = 83.2 bits (204), Expect = 6e-16, Method: Compositional matrix adjust. Identities = 93/343 (27%), Positives = 142/343 (41%), Gaps = 53/343 (15%) Query: 13 AVVSQNGQISVADIPAPVLEDGFVLVETEYSAISPGTEI-MMNGIHRPK--PVVLGYSAA 69 AV+ + + V ++ P + VL+ + I GT++ + NG + P+V G+ Sbjct: 4 AVLVEPRRFEVREVGIPEIGPADVLIRVTRAGIC-GTDLHIFNGHYAADRLPIVPGHEFC 62 Query: 70 GVIRSRGKGMEHWPEGQRVA---------CYGAPYAK-----------------HAEWLL 103 G I G + H G RV CY + A+++ Sbjct: 63 GTIAEIGASVTHLKTGMRVVADINIGCGNCYWCRRNEVLNCGEVEQIGIGRDGAFAQYVA 122 Query: 104 MPQHLMVPVPEHVSPEEASTVGLGAIAVHAVRQADLQFGETLVLIGAGILGQLIAQIARA 163 +P L++PVP+ V + V A V A R+A FG + V++GAG +G L Q+ R Sbjct: 123 LPGRLVLPVPDGVPEAVLALVEPVACVVRAARKAGAAFGRSGVVLGAGPIGNLHVQMMRL 182 Query: 164 AGCR-VIVYDLLAERCRTAESLGIRHIAVNPQRVSRHLDNLTAGMGADAVIICAGGKSGG 222 G +IV DL +ERCR A G P + + +T G GAD V+ G S Sbjct: 183 VGMAPIIVADLSSERCRMAVEAGADAAVSEPATLRSKVLEMTGGRGADFVVESVG--SSK 240 Query: 223 LVDQGLEWVRNQGKVLLVGDVKPDFSRELMFGKEAQVLISRAGGPGRYDPVYEKQGIDYP 282 L Q + VR G V G P G+ + I R V E+ + Sbjct: 241 LYRQAFDLVRKGGHVAFFGITPP--------GETIPIEILRT--------VLEENSLK-- 282 Query: 283 YGYVRWTEGRNMAEYIRLISEGDIRVKPLISSVFPLERSADAF 325 G V G +M + + L+S G R ++ +PLER +AF Sbjct: 283 -GSVAGM-GEDMHDALTLLSHGRFRTAAFTAAHYPLERIQEAF 323 >YPO3352 [ER] COG1063 Threonine dehydrogenase and related Zn-dependent dehydrogenases Length = 371 Score = 80.1 bits (196), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 82/344 (23%), Positives = 140/344 (40%), Gaps = 74/344 (21%) Query: 26 IPAPVLEDGFVLVETEYSAISPGTEIMMNGI-----------HRPKPVVLGYSAAGVIRS 74 +P P ++ +LV+ E I G G + P++ G+ G + Sbjct: 35 VPVPTIDAKEILVKVEGCGICAGDTKAFEGAPSFWGDDKQPAYIKAPMIPGHEFIGHVVG 94 Query: 75 RGKGMEHWPEGQRVA------CYGAPYAKHAEWLLMPQH--------------------- 107 G +E + G RV C+G + H ++ + +H Sbjct: 95 LGADVEGFELGDRVTSEQIVPCWGCRFCNHGQYWMCEKHDLYGFQSNVNGAMAEYLKFTK 154 Query: 108 --LMVPVPEHVSPEEASTVGLGAIAVHAVRQADLQFGETLVLIGAGILGQ-LIAQIARAA 164 + VP+H+ E+A + A A HAV++A ++ G+ +VL G+G LG +I I ++ Sbjct: 155 EAINYHVPDHLPLEKAILIEPYACAFHAVQRAKIKLGDVVVLAGSGTLGLGMIGAIKKSG 214 Query: 165 GCRVIVYDLLAERCRTAESLGIRHIAVNPQR--VSRHLDNLTAGMGADAVIICAGGKSGG 222 ++IV DL +R A+ G + +NP R V+ + +T G G D I G + Sbjct: 215 AAKLIVLDLSDDRLALAKKFG-ADLVLNPSRDDVNTLIKEMTGGYGCDIYIDATGAQKA- 272 Query: 223 LVDQGLEWVRNQGKVLLVGDVKPDFSRE---LMFGKEAQVLISRAGGPGRYDPVYEKQGI 279 V+QGL +R G + K + + + KE VL S G Sbjct: 273 -VEQGLTLLRKLGTFVEFSVFKDPVTVDWSIISDRKELDVLGSHLG-------------- 317 Query: 280 DYPYGYVRWTEGRNMAEYIRLISEGDIRVKPLISSVFPLERSAD 323 PY + EG IS GD+ + +++ + PLE+ A+ Sbjct: 318 --PYCFPLVIEG---------ISNGDLPTEGVVTHILPLEKYAE 350 >SA0239 [ER] COG1063 Threonine dehydrogenase and related Zn-dependent dehydrogenases Length = 351 Score = 79.3 bits (194), Expect = 8e-15, Method: Compositional matrix adjust. Identities = 82/356 (23%), Positives = 156/356 (43%), Gaps = 67/356 (18%) Query: 11 MKAVVSQ---NGQISVADIPAPVLEDGFVLVETEYSAISPGTEIMMNGIHRPK--PVVLG 65 MKA+V +G + + D D V ++ Y+ I GT+I H PV LG Sbjct: 1 MKALVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGIC-GTDIHTYEGHYKVNFPVTLG 59 Query: 66 YSAAGVIRSRGKGMEHWPEGQRVA-------CYGAPYAKHAEWLLMP------------- 105 + +G I G ++ + G RV C Y K ++ L Sbjct: 60 HEFSGEIVEVGADVKDFKVGDRVTSETTFYVCNECEYCKSKDYNLCNHRKGIGTQVDGVF 119 Query: 106 -------QHLMVPVPEHVSPEEASTVGLGAIAVHAVRQADLQFGETLVLIGAGILGQLIA 158 + + +P+ VS + A+ A A H V + + G+ V++G G +G L+A Sbjct: 120 TNYVIAREESLHHIPDEVSYQSAAMTEPLACAHHGVSKIQVNSGDVAVVMGPGPIGLLVA 179 Query: 159 QIARAAGCRVIVYDLLAERCR--TAESLGIRHIAVNPQR--VSRHLDNLTAGMGADAVII 214 Q+ ++ G V+V L ++ R AE+L + ++ VN Q+ + +++ +T G GAD V+ Sbjct: 180 QVLKSKGATVVVTGLDNDKVRLDKAEALHMDYV-VNLQQTDLKTYINGITDGYGADVVVE 238 Query: 215 CAGGKSGGLVDQGLEWVRNQG---KVLLVGDVKPDFSRELMFGKEAQVLISRAGGPGRYD 271 C+G QGL+ +R +G ++ + D + F E + KE V+ SR+ P ++ Sbjct: 239 CSGAVPAAR--QGLDIIRKKGFYSQIGIFKDAEIPFDMEKVIQKEITVVGSRSQKPADWE 296 Query: 272 PVYEKQGIDYPYGYVRWTEGRNMAEYIRLISEGDIRVKPLISSVFPLERSADAFRQ 327 P ++L+++G + + L++ ++ + + +A++ Sbjct: 297 P------------------------SLQLMADGLVNAEALVTKIYDISKWDEAYQH 328 >BH0189 [ER] COG1063 Threonine dehydrogenase and related Zn-dependent dehydrogenases Length = 343 Score = 78.2 bits (191), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 80/301 (26%), Positives = 136/301 (45%), Gaps = 43/301 (14%) Query: 11 MKAVVSQ---NGQISVADIPAPVLEDGFVLVETEYSAISPGTEIMMNGIHRP--KPVVLG 65 MKA+V G +V + P P V ++ +Y+ + G++I H P PV LG Sbjct: 1 MKALVKTQHGTGHFAVQEKPEPTPGKHQVKIKVKYTGVC-GSDIHTYEGHYPVAAPVTLG 59 Query: 66 YSAAGVIRSRGKGMEHWPEGQRVA-------------CYGAPY--------------AKH 98 + +G I G+G+ + G RV C Y Sbjct: 60 HEFSGEIVELGEGVTGFNVGDRVTSETTYSICGKCSYCTSGDYNLCSHRKGLGNQQDGSF 119 Query: 99 AEWLLMPQHLMVPVPEHVSPEEASTVGLGAIAVHAVRQADLQFGETLVLIGAGILGQLIA 158 A++++ Q + +P V A+ A HA+ + + G+ +V+ G G +G L A Sbjct: 120 AKYVIARQESLHHLPAGVDDRSAAMTEPLACTHHAIAKTSINKGDLVVVTGPGPIGLLAA 179 Query: 159 QIARAAGCRVIVYDLLAE--RCRTAESLGIRHIAVNPQRVS--RHLDNLTAGMGADAVII 214 Q+A++ G VI+ L + R + A+ +GI + A++ Q V + LT G GAD V+ Sbjct: 180 QVAKSHGGTVIITGLSNDQVRLKKAKEVGIDY-AIDTQEVDIKELVSELTDGYGADVVLE 238 Query: 215 CAGGKSGGLVDQGLEWVRNQGKVLLVG-DVKPD--FSRELMFGKEAQVLISRAGGPGRYD 271 C+G QG++ +R +G+ VG +P+ F+ E + KE V+ SR+ P ++ Sbjct: 239 CSGAVPAA--KQGIDLLRKKGQYAQVGLFAQPEIQFNFEKIIQKEISVVGSRSQKPADWE 296 Query: 272 P 272 P Sbjct: 297 P 297 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.138 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,119,756 Number of extensions: 1220625 Number of successful extensions: 4559 Number of sequences better than 1.0e-05: 123 Number of HSP's gapped: 4522 Number of HSP's successfully gapped: 123 Length of query: 363 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 255 Effective length of database: 25,922,359 Effective search space: 6610201545 Effective search space used: 6610201545 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)