BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0373.0012.p (354 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3382 [M] COG0438 Glycosyltransferase 114 3e-25 APE1191 [M] COG0438 Glycosyltransferase 77 4e-14 MTH1739 [C] COG0674 Pyruvate:ferredoxin oxidoreductase and relat... 71 3e-12 MTH1736 [C] COG1142 Fe-S-cluster-containing hydrogenase componen... 71 3e-12 MTH1732 [P] COG0704 Phosphate uptake regulator 71 3e-12 >BH3382 [M] COG0438 Glycosyltransferase Length = 356 Score = 114 bits (284), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 84/295 (28%), Positives = 135/295 (45%), Gaps = 10/295 (3%) Query: 1 MRILYFYKFCLLGGVTTQLANRLKFLHGR-AEVHFAFLEDYGGASAFEGYAHVCILGTPS 59 M+IL+ + + GGV T +R L+ E HF +L++ G + G A + Sbjct: 1 MKILFTF-YLATGGVVTLNRHRYHALNSEDCECHFLYLKNGSGLNTM-GNARTYVTNDDE 58 Query: 60 ELRAYIEAHDFDVIITIDTYELYEALGPAEQKKVIIHEVH---TTYEEPLRKLAESKDIL 116 +++A + A +DVI+ + L K II +V T +E K + Sbjct: 59 DIKALLYAKQYDVIVVTLDFLFVNRLRRLGYKGKIIFDVQGFGTDIKELFEKANDHFITG 118 Query: 117 PFNYVITPSDYMKEMLDQIGIPD--AYHINNCLDTELFKYEAVSGKRPPTILWVGKLDQH 174 + +++P E L P A+H +NC+ +F + + I WVG+++++ Sbjct: 119 EVDAILSPKTSHLEQLIAHHFPSTPAFHFHNCITFSMFTCKPQPKPKRTIIGWVGRIEEN 178 Query: 175 KNWESFMNIAGRLNAQFPDLHYLLVGGYTAPDEITSK-LQARVKALELR-HFEWLPKVDY 232 KNW F+ I +L + L + A + K + VK L+LR H L V Y Sbjct: 179 KNWRYFLKIGEKLLKEDSSLELWIFEDPKAANPSEKKHFHSMVKKLKLRNHMRSLQNVPY 238 Query: 233 EEMNHYYSAVAESGGVYVSTTRNESFGMTVLEAMACRCPVVVPDVGALPELLDDE 287 E M +YS + SGG ST++ E FG VLEAM C+CPVV D + + +E Sbjct: 239 ERMAEFYSLMGRSGGFLCSTSKKEGFGYAVLEAMCCKCPVVCTDSDGIKSFVINE 293 >APE1191 [M] COG0438 Glycosyltransferase Length = 363 Score = 77.0 bits (188), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 72/294 (24%), Positives = 129/294 (43%), Gaps = 26/294 (8%) Query: 67 AHDFDVIITIDTYELYEALGPAEQKKVIIHEVHTTYEE--------PLRKLAES--KDIL 116 H++DVI+ D P K ++ ++H +++ PL + K I Sbjct: 79 GHEYDVIVD-DIAHAVPWYSPLVTKTPVVAQIHHVHQDVVYIELPKPLAWIVSRAEKTIA 137 Query: 117 PF--NYVITPSDYMKEMLDQIGI-PDAYHI-NNCLDTELFKYEAVSGKRPPTILWVGKLD 172 ++ KE+ ++GI PD + N +D E KY S PTILW G++ Sbjct: 138 KIYRRFIAVSQSTKKELAKRLGIDPDRIAVVPNGVDLE--KYRPGSKDPRPTILWAGRIK 195 Query: 173 QHKNWESFMNIAGRLNAQFPDLHYLLVGGYTAPDEITSKLQARVKALELRHFEWLPKVDY 232 +KN + + + + PD +++G T E K++ K LE R +L K+ Sbjct: 196 MYKNLDHLLKAYRIVKQEIPDAQLIIIG--TGDQE--QKMRELAKKLEPRDVHFLGKMSE 251 Query: 233 EEMNHYYSAVAESGGVYVSTTRNESFGMTVLEAMACRCPVVVPDVGALPELLDDELSVSL 292 +E + + + VST+ E +G+T+ EA AC+ P + +V L + + + L Sbjct: 252 QEKIMWM----QRAWIIVSTSMIEGWGITITEAAACKIPAIAYNVPGLRDSVKHMETGIL 307 Query: 293 YASGREEECAERVAWLXXXXGLRARLKTLGEQKARKTYSIEEVGKAYMALLEQF 346 G E+ A+ +AWL LR +L A +++S + ++ +LE Sbjct: 308 VEPGNIEQLAKAIAWLLTDNSLRNKLSENAYNYA-QSFSWDNTAITFLNILEDI 360 >MTH1739 [C] COG0674 Pyruvate:ferredoxin oxidoreductase and related 2-oxoacid:ferredoxin oxidoreductases, alpha subunit Length = 383 Score = 71.2 bits (173), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 20/306 (6%), Positives = 54/306 (17%), Gaps = 39/306 (12%) Query: 64 YIEAHDFDVIITIDTYELYEALGPAEQKKVI--------IHEVHTTYEEPLRKLAESKDI 115 + + + + A + + + + + + Sbjct: 88 HEIVYAAAGL----RNPIVMANANRALSAPLSIWNDQQDSIAERDSGWMQIYVESGQEAL 143 Query: 116 L-------PFNYVITPSDYMKEMLDQI--GIPDAYHI-NNCLDTELFKYEAVSGKRPPTI 165 M + I + I + + Sbjct: 144 DSVLLSYRVSEDRDVLLPSMVCLDGFILTHTVEPVDIPSQEDVDSFL--PEFQPQVMLDP 201 Query: 166 LWVGKLDQHKNWESFMNIAGRLNAQFPDLHYLLVGGYTAPDEITSKLQARVKALELRHFE 225 L + + +M + ++ S++ R L + Sbjct: 202 DEPMSLGTFTDPDYYMEARYEVEKAMERSRKIIERACRE----FSEMFGREYGLVEEY-- 255 Query: 226 WLPKVDYEEMNHYYSAVAESGG--VYVSTTRNESFGMTVLEAMACRCPVVVPDVGALPEL 283 + + E+ S V + + P Sbjct: 256 ---RCEDAEIILVAMGSVCSTLREVIDELRDKGKAVGLLKVRVHRPFPAEEIKSAVRNAS 312 Query: 284 LDDELSVSLYASGREEECAERVAWLXXXXGLRARLKTLGEQKARKTY----SIEEVGKAY 339 L ++ S E A L + G E + Sbjct: 313 KVAVLDKNITFSVGGALYTEISALLRDREVYGFIVGLGGRDITPAHIEEIVRRTENPERS 372 Query: 340 MALLEQ 345 + + Sbjct: 373 VTWIGL 378 >MTH1736 [C] COG1142 Fe-S-cluster-containing hydrogenase components 2 Length = 268 Score = 71.2 bits (173), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 0/306 (0%), Positives = 0/306 (0%), Gaps = 39/306 (12%) Query: 64 YIEAHDFDVIITIDTYELYEALGPAEQKKVI--------IHEVHTTYEEPLRKLAESKDI 115 + K + + E L Sbjct: 88 PPLRTPHHPT----GKPVTGEYRQGLTVKRVKKGTLVDIGADKLALCREKLTVNRIMSFR 143 Query: 116 L-------PFNYVITPSDYMKEMLDQI--GIPDAYHI-NNCLDTELFKYEAVSGKRPPTI 165 + Y + + ++ + S Sbjct: 144 VVRLGKEILIEPDEPEDRYWGYEVLDTRRNLAESLKTVGADVVVATS--RNASPITSILD 201 Query: 166 LWVGKLDQHKNWESFMNIAGRLNAQFPDLHYLLVGGYTAPDEITSKLQARVKALELRHFE 225 ++ + + + ++ T ++ L Sbjct: 202 EVKTRMRGAREAAILFGGPYKGLPEIDADIWVNTLPGQC----TETVRTEEAVLATLS-- 255 Query: 226 WLPKVDYEEMNHYYSAVAESGG--VYVSTTRNESFGMTVLEAMACRCPVVVPDVGALPEL 283 Sbjct: 256 --- 312 Query: 284 LDDELSVSLYASGREEECAERVAWLXXXXGLRARLKTLGEQKARKTY----SIEEVGKAY 339 Sbjct: 313 372 Query: 340 MALLEQ 345 Sbjct: 373 378 >MTH1732 [P] COG0704 Phosphate uptake regulator Length = 268 Score = 71.2 bits (173), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 0/306 (0%), Positives = 0/306 (0%), Gaps = 39/306 (12%) Query: 64 YIEAHDFDVIITIDTYELYEALGPAEQKKVI--------IHEVHTTYEEPLRKLAESKDI 115 + K + + E L Sbjct: 88 PPLRTPHHPT----GKPVTGEYRQGLTVKRVKKGTLVDIGADKLALCREKLTVNRIMSFR 143 Query: 116 L-------PFNYVITPSDYMKEMLDQI--GIPDAYHI-NNCLDTELFKYEAVSGKRPPTI 165 + Y + + ++ + S Sbjct: 144 VVRLGKEILIEPDEPEDRYWGYEVLDTRRNLAESLKTVGADVVVATS--RNASPITSILD 201 Query: 166 LWVGKLDQHKNWESFMNIAGRLNAQFPDLHYLLVGGYTAPDEITSKLQARVKALELRHFE 225 ++ + + + ++ T ++ L Sbjct: 202 EVKTRMRGAREAAILFGGPYKGLPEIDADIWVNTLPGQC----TETVRTEEAVLATLS-- 255 Query: 226 WLPKVDYEEMNHYYSAVAESGG--VYVSTTRNESFGMTVLEAMACRCPVVVPDVGALPEL 283 Sbjct: 256 --- 312 Query: 284 LDDELSVSLYASGREEECAERVAWLXXXXGLRARLKTLGEQKARKTY----SIEEVGKAY 339 Sbjct: 313 372 Query: 340 MALLEQ 345 Sbjct: 373 378 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.137 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 27,139,062 Number of extensions: 1156942 Number of successful extensions: 2233 Number of sequences better than 1.0e-05: 31 Number of HSP's gapped: 2260 Number of HSP's successfully gapped: 31 Length of query: 354 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 247 Effective length of database: 26,037,693 Effective search space: 6431310171 Effective search space used: 6431310171 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)