BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0373.0010.p (218 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMc00502 [H] COG0684 Demethylmenaquinone methyltransferase 99 7e-21 SMb20114 [H] COG0684 Demethylmenaquinone methyltransferase 90 3e-18 RSp1231 [H] COG0684 Demethylmenaquinone methyltransferase 86 5e-17 AGl1765 [H] COG0684 Demethylmenaquinone methyltransferase 84 2e-16 YPO0082 [H] COG0684 Demethylmenaquinone methyltransferase 83 4e-16 >SMc00502 [H] COG0684 Demethylmenaquinone methyltransferase Length = 224 Score = 98.6 bits (244), Expect = 7e-21, Method: Compositional matrix adjust. Identities = 67/219 (30%), Positives = 111/219 (50%), Gaps = 16/219 (7%) Query: 2 NDQELFDTMCKTLYAAVISDTLDLLGYRNQVMRENINPVDPSWVVAGRAKTVLSVDIHHL 61 D+ L D + + A + + + G MR P W A A T ++V I Sbjct: 11 TDRSLADALAEC-GVATVHEAMGRTGLMQSYMR-------PVWRGARVAGTAVTVSIP-- 60 Query: 62 PEDPYTKEIEAVDSVKPGEIVVGCTNESRQNGLWGELLSTASKIRGGRGAIVDGLIRDTA 121 P D + + AV+ + G+I+V G +GELL+T+ +RG RG I++ +RD + Sbjct: 61 PADNWMIHV-AVEQCREGDILVVAPTSPCDAGYFGELLATSLMVRGVRGLIIEAGVRDVS 119 Query: 122 KILELDFPVFATGTKPVDSQG--RGIVIDYDIPVLCGGVLVHPGDVIFGDRDGVVVIPGA 179 ++ E+ FPV+ ++ V +QG + + ++P++C G V PGDV+ D DGV+V+P A Sbjct: 120 ELEEIGFPVW---SRFVSAQGTVKATLGSVNVPIVCAGATVAPGDVVIADDDGVMVVPKA 176 Query: 180 ITGEVFALATDKATRENHTRDELLAGFTLRQVYDKYGVL 218 + A + + +E TR+ L AG +YD G L Sbjct: 177 QAEQAVAASRARLEKERKTRERLAAGELGLDIYDMRGAL 215 >SMb20114 [H] COG0684 Demethylmenaquinone methyltransferase Length = 241 Score = 89.7 bits (221), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 62/209 (29%), Positives = 108/209 (51%), Gaps = 13/209 (6%) Query: 11 CKTLYAAVISDTLDLLGYRNQVMRENINPVDP-SWVVAGRAKTVLSV----DIHHLP--E 63 C TL A+ G RNQ + +++ PV P + + G+A T+ + D++ L + Sbjct: 19 CATLCTALFKR-----GLRNQFI-QDVRPVAPKAKNMVGQAFTLRYIPAREDLNQLSAFQ 72 Query: 64 DPYTKEIEAVDSVKPGEIVVGCTNESRQNGLWGELLSTASKIRGGRGAIVDGLIRDTAKI 123 +P + AV+ PG ++V + + + G +L + +RG G + DG RD+ +I Sbjct: 73 NPEHPQRAAVEKCPPGFVMVMDSRKDPRAASAGSILVSRLMVRGVAGVVTDGGFRDSPEI 132 Query: 124 LELDFPVFATGTKPVDSQGRGIVIDYDIPVLCGGVLVHPGDVIFGDRDGVVVIPGAITGE 183 ELD P + + + +D ++P+ CG V V PGDV+ GDR+GVVV+P + E Sbjct: 133 GELDIPAYHSRPSAPTNLTLHQALDINVPIGCGDVAVWPGDVVVGDREGVVVVPAHLADE 192 Query: 184 VFALATDKATRENHTRDELLAGFTLRQVY 212 + A A + E+ +E+L G ++ +Y Sbjct: 193 IAAEAVEMTAFEDFVTEEVLKGRSIIGLY 221 >RSp1231 [H] COG0684 Demethylmenaquinone methyltransferase Length = 216 Score = 85.9 bits (211), Expect = 5e-17, Method: Compositional matrix adjust. Identities = 64/203 (31%), Positives = 103/203 (50%), Gaps = 21/203 (10%) Query: 28 YRNQVMRENINP----VDPSWVVAGRAKTVLSVDIHHLPEDPYTKEIEAVDSVKPGEIVV 83 + Q R I+P +DP+ + GRA L+VD+ P D A+ +PG+++V Sbjct: 23 HEAQGQRGAIDPRIAALDPARRLVGRA---LTVDMR--PSDNLMIHY-ALTKARPGDVLV 76 Query: 84 GCTNESRQNGLWGELLSTASKIRGGRGAIVDGLIRDTAKILELDFPVFATG--TKPVDSQ 141 G WG++L+ A++ G G ++DG +RD I+E+ FPVF G K + Sbjct: 77 VDAKGFVDAGPWGDILTLAAQEIGMAGLVIDGAVRDADSIIEMGFPVFCRGLCIKGTNKH 136 Query: 142 GRGIVIDYDIPVLCGGVLVHPGDVIFGDRDGVVVIPGAITGEVFALATDKATRENHTRDE 201 G V ++PV+ GGV + PGDV+ GDRDG+VV+ V + + +E R+ Sbjct: 137 QPGRV---NVPVVIGGVPIQPGDVMVGDRDGLVVVAADEVDAVIRASEARERKEAEMRER 193 Query: 202 LLAGFT------LRQVYDKYGVL 218 L G T L+ ++YG++ Sbjct: 194 LKQGATMVELLGLQDTLNRYGLV 216 >AGl1765 [H] COG0684 Demethylmenaquinone methyltransferase Length = 134 Score = 83.6 bits (205), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 0/211 (0%), Positives = 0/211 (0%), Gaps = 16/211 (7%) Query: 13 TLYAAVISDTLDLLGYRNQVMRENINPVDPSWVVAGRAKTVLSVDIHHLP---------- 62 +++ + + + V + + L Sbjct: 11 ITFSSPAFAASCEKNFTVSGV-PMVTAVS----YKSFQELPKAKAPAVLQKLAQAVAAEG 65 Query: 63 -EDPYTKEIEAVDSVKPGEIVVGCTNESRQNGLWGELLSTASKIRGGRGAIVDGLIRDTA 121 + + G R + + + Sbjct: 66 FSGIQINKALSSIDAHQETSGSGRIQTLRVVARQKGAAVRIDAVFNIQAGQIADKDVIRK 125 Query: 122 KILELDFPVFATGTKPVDSQGRGIVIDYDIPVLCGGVLVHPGDVIFGDRDGVVVIPGAIT 181 Sbjct: 126 185 Query: 182 GEVFALATDKATRENHTRDELLAGFTLRQVY 212 Sbjct: 186 216 >YPO0082 [H] COG0684 Demethylmenaquinone methyltransferase Length = 301 Score = 82.8 bits (203), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 7/179 (3%), Positives = 25/179 (13%), Gaps = 11/179 (6%) Query: 29 RNQVMRENINPVDPSWVVAGRAKTVLSVDIHHLPEDPYTKEIEAVDSVKPGEIVVGCTNE 88 + Q R I + + + + + + Sbjct: 39 KAQTTRHRIVGIHTEGAYQAIYVDTPGL-----HMEEKRAIN-RLMNKAASSSIGDVELV 92 Query: 89 SRQNGLWGELLSTASKIRGGRGAIVDGLIRDTAKILELDFPVFATGTKPVDSQG--RGIV 146 + R ++ + + + Sbjct: 93 IFVVEGTRWTPDDEMVLNKLREGKAPVILAVNKVDNVQEKADL---LPHLQFLASQMNFL 149 Query: 147 IDYDIPVLCGGVLVHPGDVIFGDRDGVVVIPGAITGEVFALATDKATRENHTRDELLAG 205 I G + ++ + + L Sbjct: 150 DIVPISAETGLNVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGA 208 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.140 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,357,445 Number of extensions: 740031 Number of successful extensions: 1766 Number of sequences better than 1.0e-05: 11 Number of HSP's gapped: 1759 Number of HSP's successfully gapped: 11 Length of query: 218 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 116 Effective length of database: 26,614,363 Effective search space: 3087266108 Effective search space used: 3087266108 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 113 (48.1 bits)