BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0373.0009.p (253 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM1724_1 [EH] COG0512 Anthranilate/para-aminobenzoate synthases... 172 4e-43 STM1724_2 [E] COG0547 Anthranilate phosphoribosyltransferase 172 4e-43 TM1724 [IQR] COG1028 Dehydrogenases with different specificities... 172 4e-43 VC2021 [IQR] COG1028 Dehydrogenases with different specificities... 154 2e-37 fabG [IQR] COG1028 Dehydrogenases with different specificities (... 148 7e-36 >STM1724_1 [EH] COG0512 Anthranilate/para-aminobenzoate synthases component II Length = 197 Score = 172 bits (436), Expect = 4e-43, Method: Compositional matrix adjust. Identities = 0/245 (0%), Positives = 0/245 (0%), Gaps = 2/245 (0%) Query: 1 MRLAGKRVLVTGAARGIGLAITRRFLEEGARVVLLDRSETELHAAVDELADYTGSLMVEV 60 M + + + + A + S V Sbjct: 1 MADILLLDNIDSFTWNLADQLRTNGHNVVIYRNHIPAQTLIDRLATMKNPVLMLSPGPGV 60 Query: 61 CDLRDLAQLEQSAAKAFAHFGGIDIVVNNAGIAYREPFLDISPEHWNAVHEINVRAVFRI 120 + + GI + AY I Sbjct: 61 PSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAGEILHGKASSIEHDGQAMF 120 Query: 121 GQLAARQMXXXXXXXXXXNMSSKNGIAASAELAHYNASKAAVILLTESMAVELARYGIRV 180 LA + + A + + + + Sbjct: 121 AGLANPLP-VARYHSLVGSNVPAGLTINAHFNGMVMAVRHDADRVCGFQFHPESILTTQG 179 Query: 181 NAVAPGFVDTPLDRRLREEDGLPSHSEHTPMKRAAAPQEVANVFLFLASEEASYVTGETV 240 Sbjct: 180 - 238 Query: 241 RVDGG 245 Sbjct: 239 243 >STM1724_2 [E] COG0547 Anthranilate phosphoribosyltransferase Length = 334 Score = 172 bits (436), Expect = 4e-43, Method: Compositional matrix adjust. Identities = 15/245 (6%), Positives = 40/245 (16%), Gaps = 2/245 (0%) Query: 1 MRLAGKRVLVTGAARGIGLAITRRFLEEGARVVLLDRSETELHAAVDELADYTGSLMVEV 60 L + A + F + ++ L + Sbjct: 1 NTLQPILEKLYQAQTLTQQESHQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAAT 60 Query: 61 CDLRDLAQLEQSAAKAFAHFGGIDIVVNNAGIAYREPFLDISPEHWNAVHEINVRAVFRI 120 L + A + G N+ I+ F+ + A H + Sbjct: 61 ALLENAAPFPRPEYLFADIVGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSG 120 Query: 121 GQLAARQMXXXXXXXXXXNMSSKNGIAASAELAHYNASKAAVILLTESMAVELARYGIRV 180 L + ++ + Sbjct: 121 SSDLLAAF-GINLDMNADKSRQALDELGVCFLFAPKYHTGLRHAMPVRQQLKTRTLFNVL 179 Query: 181 NAVAPGFVDTPLDRRLREEDGLPSHSEHTPMKRAAAPQEVANVFLFLASEEASYVTGETV 240 + + + + + T V + + + Sbjct: 180 GPLINPAHPPLALIGVYSPELVLPIA-ETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAEL 238 Query: 241 RVDGG 245 Sbjct: 239 HDGEI 243 >TM1724 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 246 Score = 172 bits (436), Expect = 4e-43, Method: Compositional matrix adjust. Identities = 101/245 (41%), Positives = 142/245 (57%), Gaps = 2/245 (0%) Query: 1 MRLAGKRVLVTGAARGIGLAITRRFLEEGARVVLLDRSETELHAAVDELADYTGSLMVEV 60 MRL GK L+TGAA GIG A T F +EGA V+ D S+ L + V E G + V Sbjct: 1 MRLEGKVCLITGAASGIGKATTLLFAQEGATVIAGDISKENLDSLVKEAEGLPGKVDPYV 60 Query: 61 CDLRDLAQLEQSAAKAFAHFGGIDIVVNNAGIAYREPFLDISPEHWNAVHEINVRAVFRI 120 ++ D Q+++ K +G ID++VNNAGI + + E W+AV +N++ VF + Sbjct: 61 LNVTDRDQIKEVVEKVVQKYGRIDVLVNNAGITRDALLVRMKEEDWDAVINVNLKGVFNV 120 Query: 121 GQLAARQMXXXXXXXXXXNMSSKNGIAASAELAHYNASKAAVILLTESMAVELARYGIRV 180 Q+ M N+SS GI + +Y ASKA VI +T++ A ELA IRV Sbjct: 121 TQMVVPYM-IKQRNGSIVNVSSVVGIYGNPGQTNYAASKAGVIGMTKTWAKELAGRNIRV 179 Query: 181 NAVAPGFVDTPLDRRLREEDGLPSHSEHTPMKRAAAPQEVANVFLFLASEEASYVTGETV 240 NAVAPGF++TP+ +L E+ + S P+ R P+EVA V LFLAS+E+SYVTG+ + Sbjct: 180 NAVAPGFIETPMTEKLPEKARETALS-RIPLGRFGKPEEVAQVILFLASDESSYVTGQVI 238 Query: 241 RVDGG 245 +DGG Sbjct: 239 GIDGG 243 >VC2021 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 248 Score = 154 bits (388), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 94/246 (38%), Positives = 137/246 (55%), Gaps = 7/246 (2%) Query: 1 MRLAGKRVLVTGAARGIGLAITRRFLEEGARVVLLDRSETELHAAVDELADYTGSLMVEV 60 M L GK LVTGA+RGIG AI E GA+V+ SE+ A D L D + + V Sbjct: 5 MNLEGKVALVTGASRGIGKAIAELLAERGAKVIGTATSESGAQAISDYLGDNGKGMALNV 64 Query: 61 CDLRDLAQLEQSAAKAFA-HFGGIDIVVNNAGIAYREPFLDISPEHWNAVHEINVRAVFR 119 + + ++ KA FGG+DI+VNNAGI + + E W+ + E N+ ++FR Sbjct: 65 TNPESI----EAVLKAITDEFGGVDILVNNAGITRDNLLMRMKEEEWSDIMETNLTSIFR 120 Query: 120 IGQLAARQMXXXXXXXXXXNMSSKNGIAASAELAHYNASKAAVILLTESMAVELARYGIR 179 + + R M N+ S G +A A+Y A+KA VI T+SMA E+A G+ Sbjct: 121 LSKAVLRGMMKKRQGRII-NVGSVVGTMGNAGQANYAAAKAGVIGFTKSMAREVASRGVT 179 Query: 180 VNAVAPGFVDTPLDRRLREEDGLPSHSEHTPMKRAAAPQEVANVFLFLASEEASYVTGET 239 VN VAPGF++T + + L +E + ++ P R P+E+A+ FLAS EA+Y+TGET Sbjct: 180 VNTVAPGFIETDMTKALNDEQRTATLAQ-VPAGRLGDPREIASAVAFLASPEAAYITGET 238 Query: 240 VRVDGG 245 + V+GG Sbjct: 239 LHVNGG 244 >fabG [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 244 Score = 148 bits (374), Expect = 7e-36, Method: Compositional matrix adjust. Identities = 95/245 (38%), Positives = 128/245 (52%), Gaps = 5/245 (2%) Query: 1 MRLAGKRVLVTGAARGIGLAITRRFLEEGARVVLLDRSETELHAAVDELADYTGSLMVEV 60 M GK LVTGA+RGIG AI GA+V+ SE A D L LM+ V Sbjct: 1 MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQAISDYLGANGKGLMLNV 60 Query: 61 CDLRDLAQLEQSAAKAFAHFGGIDIVVNNAGIAYREPFLDISPEHWNAVHEINVRAVFRI 120 D A +E K A FG +DI+VNNAGI + + E WN + E N+ +VFR+ Sbjct: 61 TDP---ASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEEWNDIIETNLSSVFRL 117 Query: 121 GQLAARQMXXXXXXXXXXNMSSKNGIAASAELAHYNASKAAVILLTESMAVELARYGIRV 180 + R M + S G + A+Y A+KA +I ++S+A E+A GI V Sbjct: 118 SKAVMRAM-MKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIGFSKSLAREVASRGITV 176 Query: 181 NAVAPGFVDTPLDRRLREEDGLPSHSEHTPMKRAAAPQEVANVFLFLASEEASYVTGETV 240 N VAPGF++T + R L +D P R QE+AN FLAS+EA+Y+TGET+ Sbjct: 177 NVVAPGFIETDMTRAL-SDDQRAGILAQVPAGRLGGAQEIANAVAFLASDEAAYITGETL 235 Query: 241 RVDGG 245 V+GG Sbjct: 236 HVNGG 240 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.133 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 16,613,567 Number of extensions: 657784 Number of successful extensions: 3952 Number of sequences better than 1.0e-05: 348 Number of HSP's gapped: 3234 Number of HSP's successfully gapped: 348 Length of query: 253 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 149 Effective length of database: 26,383,695 Effective search space: 3931170555 Effective search space used: 3931170555 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 114 (48.5 bits)