BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0373.0008.p (279 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SSO2112 [R] COG2159 Predicted metal-dependent hydrolase of the T... 108 8e-24 AGc2623 [R] COG2159 Predicted metal-dependent hydrolase of the T... 97 3e-20 Rv3510c [R] COG2159 Predicted metal-dependent hydrolase of the T... 89 8e-18 MT3614 [R] COG2159 Predicted metal-dependent hydrolase of the TI... 89 8e-18 SSO1189 [R] COG2159 Predicted metal-dependent hydrolase of the T... 83 6e-16 >SSO2112 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Length = 269 Score = 108 bits (270), Expect = 8e-24, Method: Compositional matrix adjust. Identities = 57/175 (32%), Positives = 95/175 (54%), Gaps = 8/175 (4%) Query: 79 RLFGFASVDPNDPDCVGKFEAAVRELGLKGLKLAPIYQNFYPDDPKHMSLYATAEQLNVP 138 R+ F SVDP+ P+ + + +++E G+KL P Q F+P D + + +Y + + Sbjct: 77 RIVKFISVDPSKPNADEELKRSIKEFEPVGVKLHPQLQGFHPLDERALKIYEIIDNHGLI 136 Query: 139 ILWHQGTSFVPEGY-----LDASRPAMLDPIARAFPKLKMIVAHMGHPWVDECISLVRKH 193 +++H GTS + G LD RP D IA F +K+I+AH G PW +E I++ Sbjct: 137 VVFHTGTSGIGAGVKSSIRLDYGRPIYFDEIAVRFQNMKIILAHFGWPWTEEAIAIALHK 196 Query: 194 PNLYMDVSALGSRPWQFYNALVSAAEYGVQDKLLFGSDYPFFGTQQMLDALYSIN 248 PN+Y+D+S R + ++ + + DKLLFGSD+P ++ L+ S+N Sbjct: 197 PNVYLDLSGWAPR---YIPQVIWSNAKRLSDKLLFGSDFPLIRPERWLEEFKSVN 248 >AGc2623 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Length = 291 Score = 97.1 bits (240), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 71/198 (35%), Positives = 97/198 (48%), Gaps = 14/198 (7%) Query: 66 NEYVAEYCRTDPGRLFGFASVDPNDPDCVGKFEAA--VRELGLKGLKLAPIYQNFYPDDP 123 NE VA+ + + FAS+DP G EA VRE G+KG K P Q FYP+D Sbjct: 83 NEEVAKIAAENSDIMIPFASIDPAKGKA-GAREARRLVREFGVKGFKFHPTMQGFYPNDR 141 Query: 124 KHMSLYATAEQLNVPILWHQGTSFVPEGY-------LDASRPAMLDPIARAFPKLKMIVA 176 LY + L+H G + V G L S P LD +A FP + +I+A Sbjct: 142 MAYPLYEAIAEEGAITLFHTGQTGVGAGLRGGMAMRLKFSNPIHLDDVAVDFPDMPIILA 201 Query: 177 HMGHPWVDECISLVRKHPNLYMDVSALGSRPWQFYNALVSAAEYGVQDKLLFGSDYPFFG 236 H PW +E +S+ PN+Y+D+S G P F LV A ++ K+LFGSD+P Sbjct: 202 HPSFPWQEEALSVATHKPNVYIDMS--GWSPKYFPPILVQYANSLLKHKMLFGSDWPAMT 259 Query: 237 TQQMLD--ALYSINDLVE 252 ++ L+ A SI D V Sbjct: 260 PERWLNDFANISIKDEVR 277 >Rv3510c [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Length = 278 Score = 89.0 bits (219), Expect = 8e-18, Method: Compositional matrix adjust. Identities = 62/190 (32%), Positives = 94/190 (49%), Gaps = 17/190 (8%) Query: 63 VVPNEYVAEYCRTDPGRLFGFASVDPNDPDCVGKFEAAVREL------GLKGLKLAPIYQ 116 ++ N+ VAE+ R P R G A+VD + P AAVREL G GL++ P Sbjct: 66 LISNDAVAEWVRLYPNRFAGLAAVDLDRP------MAAVRELRRRVGEGFVGLRVVPWLW 119 Query: 117 NFYPDDPKHMSLYATAEQLNVPILWHQGTSFVPEGYLDASRPA-MLDPIARAFPKLKMIV 175 P D ++ L+A Q VP G + P + RP +D +A FP+L ++ Sbjct: 120 GAPPTDRRYYPLFAECVQSAVPFCTQVGHTG-PLRPSETGRPIPYIDQVALDFPELVIVC 178 Query: 176 AHMGHPWVDECISLVRKHPNLYMDVSALGSRPWQFYNALVSAAEYGV-QDKLLFGSDYPF 234 H+G+PW +E +++ RKH N+Y+D SA + + LV + Q K+LFG++YP Sbjct: 179 GHVGYPWTEEMVAVARKHENVYIDTSAYTIK--RLPGKLVRFMKTDTGQRKVLFGTNYPM 236 Query: 235 FGTQQMLDAL 244 L L Sbjct: 237 IAHTHALTGL 246 >MT3614 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Length = 278 Score = 89.0 bits (219), Expect = 8e-18, Method: Compositional matrix adjust. Identities = 62/190 (32%), Positives = 94/190 (49%), Gaps = 17/190 (8%) Query: 63 VVPNEYVAEYCRTDPGRLFGFASVDPNDPDCVGKFEAAVREL------GLKGLKLAPIYQ 116 ++ N+ VAE+ R P R G A+VD + P AAVREL G GL++ P Sbjct: 66 LISNDAVAEWVRLYPNRFAGLAAVDLDRP------MAAVRELRRRVGEGFVGLRVVPWLW 119 Query: 117 NFYPDDPKHMSLYATAEQLNVPILWHQGTSFVPEGYLDASRPA-MLDPIARAFPKLKMIV 175 P D ++ L+A Q VP G + P + RP +D +A FP+L ++ Sbjct: 120 GAPPTDRRYYPLFAECVQSAVPFCTQVGHTG-PLRPSETGRPIPYIDQVALDFPELVIVC 178 Query: 176 AHMGHPWVDECISLVRKHPNLYMDVSALGSRPWQFYNALVSAAEYGV-QDKLLFGSDYPF 234 H+G+PW +E +++ RKH N+Y+D SA + + LV + Q K+LFG++YP Sbjct: 179 GHVGYPWTEEMVAVARKHENVYIDTSAYTIK--RLPGKLVRFMKTDTGQRKVLFGTNYPM 236 Query: 235 FGTQQMLDAL 244 L L Sbjct: 237 IAHTHALTGL 246 >SSO1189 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Length = 270 Score = 82.8 bits (203), Expect = 6e-16, Method: Compositional matrix adjust. Identities = 53/192 (27%), Positives = 94/192 (48%), Gaps = 14/192 (7%) Query: 74 RTDPGRLFGFASVDPNDPDCVGKFEAAVRELGLKGLKLAPIYQNFYPDDPKH-------- 125 + +P + V+P D + E LG+ G+KL P++ F P+ + Sbjct: 72 KRNPDLYMQWGEVNPLKCDVKRELERQY-SLGIIGIKLHPVHHTFKPNAYREEEGGLKQL 130 Query: 126 MSLYATAEQLNVPILWHQGTSFVPEGYLDASRPAMLDPIARAFPKLKMIVAHMGHP-WVD 184 + +Y AE +PIL H GTS + P D IA+ FPKL++I+AH G P W + Sbjct: 131 LYIYEFAEDHGLPILIHTGTSIGVGSRNKYADPIYADDIAKDFPKLRIILAHAGRPLWYN 190 Query: 185 ECISLVRKHPNLYMDVSALGSRPWQFYNALVSAAEYGVQDKLLFGSDYPFFGTQQMLDAL 244 + R N+++++S++ P L E + DK+++GSD+P F Q + + Sbjct: 191 TAFHMARFTQNIFLEISSIP--PKNILKVLPRIHE--ISDKVIYGSDFPAFKGQDLAEYA 246 Query: 245 YSINDLVEGTKL 256 Y + ++++ K+ Sbjct: 247 YQVYNVLKNEKI 258 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.140 0.439 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,500,607 Number of extensions: 973006 Number of successful extensions: 1550 Number of sequences better than 1.0e-05: 10 Number of HSP's gapped: 1537 Number of HSP's successfully gapped: 10 Length of query: 279 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 174 Effective length of database: 26,268,361 Effective search space: 4570694814 Effective search space used: 4570694814 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 115 (48.9 bits)