BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0370.0042.p (320 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Rv3730c [L] COG3285 Predicted eukaryotic-type DNA primase 128 1e-29 MT3835 [L] COG3285 Predicted eukaryotic-type DNA primase 128 1e-29 mll9625_2 [L] COG3285 Predicted eukaryotic-type DNA primase 127 2e-29 SMc03959_2 [L] COG3285 Predicted eukaryotic-type DNA primase 127 3e-29 Rv0269c [L] COG3285 Predicted eukaryotic-type DNA primase 125 7e-29 >Rv3730c [L] COG3285 Predicted eukaryotic-type DNA primase Length = 346 Score = 128 bits (322), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 89/286 (31%), Positives = 138/286 (48%), Gaps = 14/286 (4%) Query: 29 AAVKGSITVDGQEIIITNPDKPLWPEVG---ITKRIYLQKLAALSPYLLRYSRDRLLTVI 85 AA + VDG + +T+PD+ +P++G +R+ A +L RDR + Sbjct: 2 AAAAEELDVDGIAVRLTSPDRMYFPKLGSHGTKRRLVEYYFAVAGGPMLTALRDRPTHLQ 61 Query: 86 RYPHGVPGMSFYQKNAPEPLPDFVRTATHDNITYIVLQGL-----PELLWLGNLAALEFH 140 R+P GV G YQK P PD+++T + + L ++W + + H Sbjct: 62 RFPDGVDGEQIYQKRIPRHRPDYLQTCRVTFPSGRMADALKVTHPAAIVWAAQMGTITLH 121 Query: 141 P-SLHYVGSTLPCEWMIDLDP---SLEVEPRIMEAAAVVGEVLQSLGIASVPKTSGATGV 196 P + + P E IDLDP + VE R + A V+ VL LG+ PKTSG G+ Sbjct: 122 PWQVRCPDTEHPDELRIDLDPQPGTGFVEARTV-AVDVLRSVLDDLGLVGYPKTSGGRGI 180 Query: 197 QIIVPIRPGVTFDGLRRIGHFVGRYVTEKRPDLFTLERLKKNRGDKIYFDYLQHYGGKTL 256 + + I F +RR G + R V + PD T K+ RG +I+ D+ Q+ +T+ Sbjct: 181 HVFLRIATDWDFVEVRRAGIALAREVERRAPDAVTTSWWKEERGARIFIDFNQNARDRTM 240 Query: 257 AAPYTPRARPLATVSTPLLWEEVQHNVSPADFHLLNIEERLSIMGD 302 A+ Y+ R P+ATVS PL WEE+ P D+ + + E + I D Sbjct: 241 ASAYSVRPTPIATVSMPLTWEELA-GADPDDYTMTTVPELVKIRDD 285 >MT3835 [L] COG3285 Predicted eukaryotic-type DNA primase Length = 346 Score = 128 bits (322), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 89/286 (31%), Positives = 138/286 (48%), Gaps = 14/286 (4%) Query: 29 AAVKGSITVDGQEIIITNPDKPLWPEVG---ITKRIYLQKLAALSPYLLRYSRDRLLTVI 85 AA + VDG + +T+PD+ +P++G +R+ A +L RDR + Sbjct: 2 AAAAEELDVDGIAVRLTSPDRMYFPKLGSHGTKRRLVEYYFAVAGGPMLTALRDRPTHLQ 61 Query: 86 RYPHGVPGMSFYQKNAPEPLPDFVRTATHDNITYIVLQGL-----PELLWLGNLAALEFH 140 R+P GV G YQK P PD+++T + + L ++W + + H Sbjct: 62 RFPDGVDGEQIYQKRIPRHRPDYLQTCRVTFPSGRMADALKVTHPAAIVWAAQMGTITLH 121 Query: 141 P-SLHYVGSTLPCEWMIDLDP---SLEVEPRIMEAAAVVGEVLQSLGIASVPKTSGATGV 196 P + + P E IDLDP + VE R + A V+ VL LG+ PKTSG G+ Sbjct: 122 PWQVRCPDTEHPDELRIDLDPQPGTGFVEARTV-AVDVLRSVLDDLGLVGYPKTSGGRGI 180 Query: 197 QIIVPIRPGVTFDGLRRIGHFVGRYVTEKRPDLFTLERLKKNRGDKIYFDYLQHYGGKTL 256 + + I F +RR G + R V + PD T K+ RG +I+ D+ Q+ +T+ Sbjct: 181 HVFLRIATDWDFVEVRRAGIALAREVERRAPDAVTTSWWKEERGARIFIDFNQNARDRTM 240 Query: 257 AAPYTPRARPLATVSTPLLWEEVQHNVSPADFHLLNIEERLSIMGD 302 A+ Y+ R P+ATVS PL WEE+ P D+ + + E + I D Sbjct: 241 ASAYSVRPTPIATVSMPLTWEELA-GADPDDYTMTTVPELVKIRDD 285 >mll9625_2 [L] COG3285 Predicted eukaryotic-type DNA primase Length = 299 Score = 127 bits (320), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 81/264 (30%), Positives = 133/264 (50%), Gaps = 12/264 (4%) Query: 42 IIITNPDKPLWPEVG----ITKRIYLQKLAALSPYLLRYSRDRLLTVIRYPHGVPGMSFY 97 ++I+NPDKPLWP+ +TK + A+ P+L+ + R R ++IR P G G F+ Sbjct: 1 VLISNPDKPLWPDANDGIPVTKEDLARYYEAVGPWLIEHIRGRPCSIIRAPDGFGGEQFF 60 Query: 98 QKNAPEPLPDFVRTAT--HDNITYIVLQGLPELLWLGNLAALEFHP-SLHYVGSTLPCEW 154 Q++A + + D Y+ + + L + + ALE HP + +P Sbjct: 61 QRHAMPGTSNLLELVEVFGDKKPYLQIDRVEGLAAVAQIGALELHPWNCEPQQPEVPGRL 120 Query: 155 MIDLDPSLEVEPR-IMEAAAVVGEVLQSLGIASVPKTSGATGVQIIVPI----RPGVTFD 209 + DLDP +V + ++EAA + L LG+ S KT+G G+ ++ P+ R +T+ Sbjct: 121 VFDLDPGPDVPFKTVVEAAKEMRARLDDLGLVSFCKTTGGKGLHVVTPLAVTKRSKLTWP 180 Query: 210 GLRRIGHFVGRYVTEKRPDLFTLERLKKNRGDKIYFDYLQHYGGKTLAAPYTPRARPLAT 269 + H V + P L+ + K R +I+ DYL++ T AP +PRARP AT Sbjct: 181 EAKGFAHDVCLQMARDNPALYLTKMAKNQRIGRIFLDYLRNDRMATAVAPLSPRARPGAT 240 Query: 270 VSTPLLWEEVQHNVSPADFHLLNI 293 VS PL W +V+ ++ P F L + Sbjct: 241 VSMPLTWAQVKTDLDPKRFTLRTV 264 >SMc03959_2 [L] COG3285 Predicted eukaryotic-type DNA primase Length = 289 Score = 127 bits (318), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 81/272 (29%), Positives = 140/272 (51%), Gaps = 15/272 (5%) Query: 42 IIITNPDKPLWPEVGITKRIYLQKLAALSPYLLRYSRDRLLTVIRYPHGVPGMSFYQKNA 101 I I++PD+ L+ GITK + A ++ +L ++ D ++++R P G F+QK+A Sbjct: 1 IRISHPDRVLFEGQGITKIDLARYYAVVAERMLPFAADHPVSLVRCPQGGERHCFFQKHA 60 Query: 102 PEPLPDFVRT-----ATHDNITYIVLQGLPELLWLGNLAALEFHPSLHYVGSTL-----P 151 + P+ +R ++ D Y+ + L+ + LEFH GS++ P Sbjct: 61 SDGFPEAIREVPITESSGDTENYMYIHDAKGLVAAVQMGTLEFH----IWGSSIDRLEKP 116 Query: 152 CEWMIDLDPSLEVEPRIMEAAAV-VGEVLQSLGIASVPKTSGATGVQIIVPIRPGVTFDG 210 + DLDP V+ ++AAA+ + + L +G+ +VP +G GV +IVP+RP ++ Sbjct: 117 DRLVFDLDPDPSVDFETVKAAALRLRDELAEIGLKTVPMVTGGKGVHVIVPLRPHAEWEE 176 Query: 211 LRRIGHFVGRYVTEKRPDLFTLERLKKNRGDKIYFDYLQHYGGKTLAAPYTPRARPLATV 270 + + R + E+ PD F K R KI+ D+L++ G T APY+ RAR V Sbjct: 177 AKGFAKALARSIAERDPDNFVATMSKAKRKGKIFIDWLRNDRGATAIAPYSTRARSGGPV 236 Query: 271 STPLLWEEVQHNVSPADFHLLNIEERLSIMGD 302 +TP+ W+E+Q + F + +I ER+ D Sbjct: 237 ATPVGWDELQGLEAANGFQIPDIIERIEAGTD 268 >Rv0269c [L] COG3285 Predicted eukaryotic-type DNA primase Length = 397 Score = 125 bits (315), Expect = 7e-29, Method: Compositional matrix adjust. Identities = 89/280 (31%), Positives = 142/280 (50%), Gaps = 13/280 (4%) Query: 34 SITVDGQEIIITNPDKPLWP----EVGITKRIYLQKLAALSPYLLRYSRDRLLTVIRYPH 89 S+ V G+++I+T+P + ++P G TK ++ A++ +R R + + R+ Sbjct: 9 SLDVHGRQVIVTHPGRVVFPAHNDRKGYTKFDLVRYYLAVAEGAMRGVAGRPMILKRFVK 68 Query: 90 GVPGMSFYQKNAPEPLPDFVRTA-----THDNITYIVLQGLPELLWLGNLAALEFHPSLH 144 G+ + +QK AP PD+V A + + V+ L W+ NL ++ +P Sbjct: 69 GISAEAVFQKRAPANRPDWVDVAELHYASGRSAAEAVIHDAAGLAWVINLGCVDLNPHPV 128 Query: 145 YVGS-TLPCEWMIDLDPSLEVE-PRIMEAAAVVGEVLQSLGIASVPKTSGATGVQIIVPI 202 G P E +DLDP V R++E A VV EVL+ G+ + PKTSG+ G + I Sbjct: 129 LAGDLDHPDELRVDLDPMPGVAWQRVVEVALVVREVLEDYGLTAWPKTSGSRGFHVYARI 188 Query: 203 RPGVTFDGLRRIGHFVGRYVTEKRPDLFTLERLKKNRGDKIYFDYLQHYGGKTLAAPYTP 262 P +F +R V R V + PD T K+ R + ++ D+ Q+ +T+A+ Y+ Sbjct: 189 APCWSFPQVRLAAQTVAREVERRLPDAATSRWWKEER-EGVFVDFNQNAKDRTVASAYSV 247 Query: 263 RARPLATVSTPLLWEEVQHNVSPADFHLLNIEERLSIMGD 302 RA P A VSTPL WEEV PA F + + RL+ +GD Sbjct: 248 RATPDARVSTPLHWEEVP-GCDPAVFTMATVPSRLADIGD 286 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.140 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 25,683,583 Number of extensions: 1162282 Number of successful extensions: 2517 Number of sequences better than 1.0e-05: 15 Number of HSP's gapped: 2493 Number of HSP's successfully gapped: 15 Length of query: 320 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 214 Effective length of database: 26,153,027 Effective search space: 5596747778 Effective search space used: 5596747778 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)