BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0370.0038.p (320 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AF1725 [L] COG1793 ATP-dependent DNA ligase 147 3e-35 mll4606_1 [L] COG1793 ATP-dependent DNA ligase 126 4e-29 MT0965_2 [L] COG1793 ATP-dependent DNA ligase 105 1e-22 Rv0938_2 [L] COG1793 ATP-dependent DNA ligase 105 1e-22 AGpA74_1 [L] COG1793 ATP-dependent DNA ligase 100 4e-21 >AF1725 [L] COG1793 ATP-dependent DNA ligase Length = 313 Score = 147 bits (370), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 98/296 (33%), Positives = 157/296 (53%), Gaps = 20/296 (6%) Query: 24 IAQIKWDGVRMLSYCD--GSEAELINRRGNRRTLQYPEIADPRAYCRAGSVILDGEVIAL 81 I ++KWDG R L++ D G L NRR T +YPEI A R + ILDGEV+ + Sbjct: 25 IYEVKWDGTRCLAFVDVEGKRVRLQNRRLLDITYRYPEIKLLDAVER--NAILDGEVVVI 82 Query: 82 SGGKPSFHEVMRRDSLKNAAAIKAVSPQVPVLYMVFDILYCNGTWLLDQPLSRRQQMLGE 141 GKPSF + +R+ + + I+ +S +P +Y+VFD+LYC+G W++D L R+++L E Sbjct: 83 KDGKPSFPLLQKREHVDSRFKIEILSKTIPAVYIVFDVLYCDG-WVVDLELMERKKILSE 141 Query: 142 MLLPHPHVQAVPSYADPA--ELYAAAVRQGLEGIVCKDSGSTYALGGKDKRW--LKRKII 197 +L V V + + E Y AV GLEGIV K Y +G + + W +K++ Sbjct: 142 VLQSRGRV-VVEEWIEGKGEEFYRKAVEMGLEGIVAKKKDGRYLIGKRSRLWKKIKKRNT 200 Query: 198 FDVTAVA--GGVTFRDGTVNAVLLGLYDNEGKLHYIGHAGAG-RMTVQDWRDLTEQAHTL 254 D V G R T +++L +Y++ G+L ++G+ G G +W +E+ + Sbjct: 201 LDCVIVGWIEGEGERAKTFGSLVLAVYED-GRLKHVGNVGTGFDSEFLNW--FSERLREI 257 Query: 255 ASETMPFAELPERSKGAFWIKPQLVFKVHYLEWNASGTLRHPS-IQARVELAPQHC 309 P E+ +K W+KP+ V +V +LE+ G LR P ++ R + +P C Sbjct: 258 EV-AKPHFEIE--TKNVHWVKPRYVCEVEFLEFTEDGKLRAPVFLRLREDKSPDEC 310 >mll4606_1 [L] COG1793 ATP-dependent DNA ligase Length = 547 Score = 126 bits (317), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 95/318 (29%), Positives = 156/318 (49%), Gaps = 24/318 (7%) Query: 9 FEPVLAS---QLPEGGQWIAQIKWDGVRMLSYCDGSEAELINRRGNRRTLQYP-EIADPR 64 EP LA+ P G W+ ++K+DG RM ++ G++ L+ R G T ++ EI Sbjct: 222 IEPQLATLERDAPPGKDWLHEVKFDGYRMQAHIAGTDVRLLTRTGLDWTEKFGGEIIAEL 281 Query: 65 AYCRAGSVILDGEVIALSGGKPSFHEVMRRD-SLKNAAAIKAVSPQVPVLYMVFDILYCN 123 A + I+DGE++ L+ S ++++D S K A LY VFD++ + Sbjct: 282 AGLKCSDAIIDGEIVVLADSGVSSFALLQQDLSAKRANRF---------LYYVFDLMRLD 332 Query: 124 GTWLLDQPLSRRQQMLGEML---LPHPHVQAVPSYADPAE-LYAAAVRQGLEGIVCKDSG 179 G L +PL R+Q L E+L +P V+ + +P + + A R GLEG+V K + Sbjct: 333 GKDLRAEPLVERKQALQELLGKQAENPAVRFSDHFFEPGKIMLEHACRMGLEGVVSKRAD 392 Query: 180 STYALGGKDKRWLKRKIIFDVTAVAGGVTFRDGT---VNAVLLGLYDNEGKLHYIGHAGA 236 + Y G+ W+K K V GG D T + ++L+G Y+ GKL Y G G Sbjct: 393 APYR-SGRGPTWVKSKCTARQEFVIGGYLPSDKTGRGLRSLLVGYYEG-GKLQYAGRVGT 450 Query: 237 GRMTVQDWRDLTEQAHTLASETMPFAELPERSKGAFWIKPQLVFKVHYLEWNASGTLRHP 296 G +V+ +L + L ++ PF + + KG W+KP+LV +V + W + +RH Sbjct: 451 G-FSVKGATELKTRLDALQAKASPFDKAVPKGKGLVWVKPELVGEVEFRSWTSDRIVRHA 509 Query: 297 SIQARVELAPQHCLLSQR 314 S Q E P ++ ++ Sbjct: 510 SFQGLREDKPAEDVVQEK 527 >MT0965_2 [L] COG1793 ATP-dependent DNA ligase Length = 468 Score = 105 bits (261), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 95/312 (30%), Positives = 140/312 (44%), Gaps = 32/312 (10%) Query: 2 KLQPVVPFE---PVLASQLPEGG----QWIAQIKWDGVRMLSYCDGSEAELINRRGNRRT 54 K Q V F+ P+LA+ G QW + KWDG R+L D L +R G T Sbjct: 157 KDQKVFEFDNLAPMLATHGTVAGLKASQWAFEGKWDGYRLLVEADHGAVRLRSRSGRDVT 216 Query: 55 LQYPEIADPRAYCRAGSVILDGEVIAL-SGGKPSFHEVMRRDSLKNAAAIKAVSPQVPVL 113 +YP++ V+LDGE + L S G PSF ++ R V Sbjct: 217 AEYPQLRALAEDLADHHVVLDGEAVVLDSSGVPSFSQMQNRGR------------DTRVE 264 Query: 114 YMVFDILYCNGTWLLDQPLSRRQQMLGEMLLPHPHVQAVPSY--ADPAELYAAAVRQGLE 171 + FD+LY +G LL R+++L L + VP D A+ +A + + G E Sbjct: 265 FWAFDLLYLDGRALLGTRYQDRRKLL--ETLANATSLTVPELLPGDGAQAFACSRKHGWE 322 Query: 172 GIVCKDSGSTYALGGKDKRWLKRKIIFDVTAVAGGVTFRDG----TVNAVLLGLYDNEGK 227 G++ K S Y G + W+K K V GG +G V ++L+G+ G Sbjct: 323 GVIAKRRDSRYQPGRRCASWVKDKHWNTQEVVIGGWRAGEGGRSSGVGSLLMGI-PGPGG 381 Query: 228 LHYIGHAGAGRMTVQDWRDLTEQAHTLASETMPF-AELPER-SKGAFWIKPQLVFKVHYL 285 L + G G G ++ ++ +L E L ++ PF LP R +KG ++KP LV +V Y Sbjct: 382 LQFAGRVGTG-LSERELANLKEMLAPLHTDESPFDVPLPARDAKGITYVKPALVAEVRYS 440 Query: 286 EWNASGTLRHPS 297 EW G LR S Sbjct: 441 EWTPEGRLRQSS 452 >Rv0938_2 [L] COG1793 ATP-dependent DNA ligase Length = 468 Score = 105 bits (261), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 95/312 (30%), Positives = 140/312 (44%), Gaps = 32/312 (10%) Query: 2 KLQPVVPFE---PVLASQLPEGG----QWIAQIKWDGVRMLSYCDGSEAELINRRGNRRT 54 K Q V F+ P+LA+ G QW + KWDG R+L D L +R G T Sbjct: 157 KDQKVFEFDNLAPMLATHGTVAGLKASQWAFEGKWDGYRLLVEADHGAVRLRSRSGRDVT 216 Query: 55 LQYPEIADPRAYCRAGSVILDGEVIAL-SGGKPSFHEVMRRDSLKNAAAIKAVSPQVPVL 113 +YP++ V+LDGE + L S G PSF ++ R V Sbjct: 217 AEYPQLRALAEDLADHHVVLDGEAVVLDSSGVPSFSQMQNRGR------------DTRVE 264 Query: 114 YMVFDILYCNGTWLLDQPLSRRQQMLGEMLLPHPHVQAVPSY--ADPAELYAAAVRQGLE 171 + FD+LY +G LL R+++L L + VP D A+ +A + + G E Sbjct: 265 FWAFDLLYLDGRALLGTRYQDRRKLL--ETLANATSLTVPELLPGDGAQAFACSRKHGWE 322 Query: 172 GIVCKDSGSTYALGGKDKRWLKRKIIFDVTAVAGGVTFRDG----TVNAVLLGLYDNEGK 227 G++ K S Y G + W+K K V GG +G V ++L+G+ G Sbjct: 323 GVIAKRRDSRYQPGRRCASWVKDKHWNTQEVVIGGWRAGEGGRSSGVGSLLMGI-PGPGG 381 Query: 228 LHYIGHAGAGRMTVQDWRDLTEQAHTLASETMPF-AELPER-SKGAFWIKPQLVFKVHYL 285 L + G G G ++ ++ +L E L ++ PF LP R +KG ++KP LV +V Y Sbjct: 382 LQFAGRVGTG-LSERELANLKEMLAPLHTDESPFDVPLPARDAKGITYVKPALVAEVRYS 440 Query: 286 EWNASGTLRHPS 297 EW G LR S Sbjct: 441 EWTPEGRLRQSS 452 >AGpA74_1 [L] COG1793 ATP-dependent DNA ligase Length = 373 Score = 100 bits (248), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 0/307 (0%), Positives = 0/307 (0%), Gaps = 21/307 (6%) Query: 18 PEGGQWIAQIKWDGVRMLSYCDGSEAELINRRGNRRTLQYPEIADPRAYCRAGSVILDGE 77 + + E +L + N + + + Sbjct: 321 IGPVDQYREQADAFAEAVLSGKPLETDLGDAIANTKAIDALFRSAASGRWE-- 378 Query: 78 VIALS-GGKPSFHEVMRRDSLKNAAAIKAVSPQVPVLYMVFDILYCNGTWLLDQPLSRRQ 136 Sbjct: 379 --------- 429 Query: 137 QMLGEMLL---PHPHVQAVPSYADPAE-LYAAAVRQGLEGIVCKDSGSTYALGGKDKRWL 192 Sbjct: 430 - 488 Query: 193 KRKIIFDVTAVAGGVTFRDGTVNAVLLGLYDNEGKLHYIGHAGAGRMTVQDWRDLTEQAH 252 Sbjct: 489 -- 546 Query: 253 TLASETMPFAEL--PERSKGAFWIKPQLVFKVHYLEWNASGTLRHPSIQARVELAPQHCL 310 Sbjct: 547 606 Query: 311 LSQRDAS 317 Sbjct: 607 613 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.136 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 25,498,351 Number of extensions: 1110906 Number of successful extensions: 1681 Number of sequences better than 1.0e-05: 34 Number of HSP's gapped: 1609 Number of HSP's successfully gapped: 34 Length of query: 320 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 214 Effective length of database: 26,153,027 Effective search space: 5596747778 Effective search space used: 5596747778 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)