BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0370.0031.p (349 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1259.10 [O] COG0533 Metal-dependent proteases with possible ... 123 5e-28 YDL104c [O] COG0533 Metal-dependent proteases with possible chap... 103 3e-22 YKR038c [O] COG0533 Metal-dependent proteases with possible chap... 92 1e-18 SPBC16D10.03 [O] COG0533 Metal-dependent proteases with possible... 90 5e-18 >SPCC1259.10 [O] COG0533 Metal-dependent proteases with possible chaperone activity Length = 104 Score = 123 bits (308), Expect = 5e-28, Method: Compositional matrix adjust. Identities = 0/349 (0%), Positives = 0/349 (0%), Gaps = 32/349 (9%) Query: 14 LAIETSCDETSVAVVK--DGCEVLSNMISSQIETHR------AFGGVVPEVASRKHVEVI 65 + + + + L + + F + Sbjct: 42 MVSRHTKPTQADPQGGIKNKEAALHVSNVA-VVDSNGKPTRVGFKIEGDKKVRVAKTTGE 100 Query: 66 TLVIEGALK-AAGVQPEQLTAVAVTQXXXXXXXXXXXXXXXXXXXXXWGKPLIGTHHIAG 124 Sbjct: 101 160 Query: 125 HIYANRLVKELQYPNMTLVVSGGHTELVSMEREGKFRIIGRTRDDAVGEAYDKVARALGF 184 Sbjct: 161 220 Query: 185 PYPGGPHVDXXXXXXXXXXXXXXXWLEP----------DSYDFSFSGLKSAVLNVVNQSK 234 Sbjct: 221 280 Query: 235 MKGLAPDVAGIARGFQESVVEVLVEKAVRAVK---ATQAKQLLLSGGVAANKGLREALTA 291 Sbjct: 281 -- 338 Query: 292 RC-------EAEGIELIIPPPVFCTDNAAMIGAAAYVKWRHDGSTPLDM 333 Sbjct: 339 387 >YDL104c [O] COG0533 Metal-dependent proteases with possible chaperone activity Length = 407 Score = 103 bits (258), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 95/341 (27%), Positives = 148/341 (43%), Gaps = 31/341 (9%) Query: 13 ILAIETSCDETSVAVVKDGCEVLS-NMISSQIETHRAF--GGVVPEVASRKHVEVITLVI 69 +LAIETSCD+T V+V+ + + N++++ +T + GG++P A H I + Sbjct: 35 VLAIETSCDDTCVSVLDRFSKSAAPNVLANLKDTLDSIDEGGIIPTKAHIHHQARIGPLT 94 Query: 70 EGALKAAGVQPEQLTAVAVTQXXXXXXXXXXXXXXXXXXXXXWGKPLIGTHHIAGHIYAN 129 E AL + + E + + VT+ W KPLIG HH+ GH+ Sbjct: 95 ERALIESNAR-EGIDLICVTRGPGMPGSLSGGLDFAKGLAVAWNKPLIGVHHMLGHLLIP 153 Query: 130 RLV---KELQYPNMTLVVSGGHTELVSMEREGKFRIIGRTRDDAVGEAYDKVARALGFPY 186 R+ K Q+P ++L+VSGGHT V I+ T D AVG++ DK R LGF Sbjct: 154 RMGTNGKVPQFPFVSLLVSGGHTTFVLSRAIDDHEILCDTIDIAVGDSLDKCGRELGFKG 213 Query: 187 PGGPHVDXXXXXXXXXXXXXXXWLEPDS-----------YDFSFSGLKSAV---LNVVNQ 232 LE S FSFS +A+ L + + Sbjct: 214 TMIAREMEKFINQDINDQDFALKLEMPSPLKNSASKRNMLSFSFSAFITALRTNLTKLGK 273 Query: 233 SKMKGLAP-DVAGIARGFQESVVEVLVEKAVRAVKA-----TQAKQLLLSGGVAANKGLR 286 ++++ L ++ IA QESV + ++ K +K+ ++ + SGGV++N+ LR Sbjct: 274 TEIQELPEREIRSIAYQVQESVFDHIINKLKHVLKSQPEKFKNVREFVCSGGVSSNQRLR 333 Query: 287 EALTARC----EAEGIELIIPPPVFCTDNAAMIGAAAYVKW 323 L PP C+DN+ MIG A W Sbjct: 334 TKLETELGTLNSTSFFNFYYPPMDLCSDNSIMIGWAGIEIW 374 >YKR038c [O] COG0533 Metal-dependent proteases with possible chaperone activity Length = 421 Score = 92.0 bits (227), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 75/297 (25%), Positives = 125/297 (42%), Gaps = 24/297 (8%) Query: 51 GVVPEVASRKHVEVITLVIEGALKAAGVQPEQLT--AVAVTQXXXXXXXXXXXXXXXXXX 108 G +P +R H +I+ AL A ++ L + T+ Sbjct: 104 GFLPRDTARHHRNWCIRLIKQALAEADIKSPTLDIDVICFTKGPGMGAPLHSVVIAARTC 163 Query: 109 XXXWGKPLIGTHHIAGHIYANRLVKELQYPNMTLVVSGGHTELVSMEREGKFRIIGRTRD 168 W PL+G +H GHI R + + Q P + L VSGG+T++++ E ++RI G T D Sbjct: 164 SLLWDVPLVGVNHCIGHIEMGREITKAQNP-VVLYVSGGNTQVIAYS-EKRYRIFGETLD 221 Query: 169 DAVGEAYDKVARALGFPYPGGP--HVDXXXXXXXXXXXXXXXWLEPDSYDFSFSGLKSAV 226 A+G D+ AR L P P +++ D S SG+ +++ Sbjct: 222 IAIGNCLDRFARTLKIPNEPSPGYNIEQLAKKAPHKENLVELPYTVKGMDLSMSGILASI 281 Query: 227 LNVVNQSKMKGLAPD--------------VAGIARGFQESVVEVLVEKAVRAVKATQAKQ 272 +++ + KG + V + QE++ +LVE RA+ + Q Sbjct: 282 -DLLAKDLFKGNKKNKILFDKTTGEQKVTVEDLCYSLQENLFAMLVEITERAMAHVNSNQ 340 Query: 273 LLLSGGVAANKGLREALTARCE--AEGIELIIPPPVFCTDNAAMIGAAAYVKWRHDG 327 +L+ GGV N L+E + C+ A G ++ FC DN MI A +++R G Sbjct: 341 VLIVGGVGCNVRLQEMMAQMCKDRANG-QVHATDNRFCIDNGVMIAQAGLLEYRMGG 396 >SPBC16D10.03 [O] COG0533 Metal-dependent proteases with possible chaperone activity Length = 346 Score = 90.1 bits (222), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 79/327 (24%), Positives = 135/327 (41%), Gaps = 21/327 (6%) Query: 9 QPVLILAIETSCDETSVAVV----KDGCEVLSNMISSQIETHRAFGGVVPEVASRKHVEV 64 +P++ L +E S ++ V ++ ++L+N+ + I G +P ++ H Sbjct: 3 KPLIALGLEGSANKLGVGIILHDTNGSAKILANVRHTYITPPGQ--GFLPSDTAKHHRAW 60 Query: 65 ITLVIEGALKAAGVQPEQLTAVAVTQXXXXXXXXXXXXXXXXXXXXXWGKPLIGTHHIAG 124 I +I+ A A + + + + T+ KPL+ +H G Sbjct: 61 IIPLIKQAFAEAKISFKDIDCICFTKGPGIGAPLNSVALCARMLSLIHKKPLVAVNHCIG 120 Query: 125 HIYANRLVKELQYPNMTLVVSGGHTELVSMEREGKFRIIGRTRDDAVGEAYDKVARALGF 184 HI R + Q P + L VSGG+T++++ E K+RI G T D A+G D+ AR +G Sbjct: 121 HIEMGREITGAQNP-VVLYVSGGNTQVIAYS-EKKYRIFGETLDIAIGNCLDRFARIIGL 178 Query: 185 PYPGGPHVDXXXXXXXXXXXXXXXWLEPDSYDFSFSGLKSAV-------LNVVNQSKMKG 237 P + + D SFSGL S V L+ N S + Sbjct: 179 SNAPSPGYNIMQEAKKGKRFIELPY-TVKGMDCSFSGLLSGVEAAATELLDPKNPSSVTK 237 Query: 238 LAPDVAGIARGFQESVVEVLVEKAVRAVKATQAKQLLLSGGVAANKGLREALTARCEAEG 297 + QE+ +LVE RA+ +A +L+ GGV N+ L++ + G Sbjct: 238 -----QDLCYSLQETGFAMLVEITERAMAHIRADSVLIVGGVGCNERLQQMMAEMSSDRG 292 Query: 298 IELIIPPPVFCTDNAAMIGAAAYVKWR 324 ++ FC DN MI A + ++ Sbjct: 293 ADVFSTDERFCIDNGIMIAQAGLLAYK 319 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.133 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,678,622 Number of extensions: 796695 Number of successful extensions: 3352 Number of sequences better than 1.0e-05: 4 Number of HSP's gapped: 3343 Number of HSP's successfully gapped: 4 Length of query: 349 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 242 Effective length of database: 26,037,693 Effective search space: 6301121706 Effective search space used: 6301121706 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 116 (49.3 bits)