BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0370.0027.p (89 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ydiF [R] COG0488 ATPase components of ABC transporters with d... 49 2e-06 SA1852 [R] COG0488 ATPase components of ABC transporters with du... 47 7e-06 lin2179 [R] COG0488 ATPase components of ABC transporters with d... 47 7e-06 >BS_ydiF [R] COG0488 ATPase components of ABC transporters with duplicated ATPase domains Length = 531 Score = 48.5 bits (114), Expect = 2e-06, Method: Compositional matrix adjust. Identities = 0/91 (0%), Positives = 0/91 (0%), Gaps = 3/91 (3%) Query: 1 VPAAEKG-AALSYEAEKQAKREERNRQRRISELEENIAVLEEDIARIENEMTRPEVFQDY 59 Sbjct: 551 610 Query: 60 VALQE-HESELKLKKEQLGNLFSEWENLADE 89 Sbjct: 611 - 640 >SA1852 [R] COG0488 ATPase components of ABC transporters with duplicated ATPase domains Length = 642 Score = 47.0 bits (110), Expect = 7e-06, Method: Compositional matrix adjust. Identities = 30/81 (37%), Positives = 50/81 (61%), Gaps = 10/81 (12%) Query: 11 SYEAEKQAKREERNRQRRISELEENIAVLEEDIARIENEMTRPEVFQDY-----VALQEH 65 SY +K+ +RE+R +R+ISE E I LE I +I+ ++T+PEV+ + +A+Q+ Sbjct: 563 SYHNQKEQRREQRKLERQISECENEIETLETTILQIDEQLTQPEVYNNPQKANELAIQKQ 622 Query: 66 ESELKLKKEQLGNLFSEWENL 86 +SE KL+ + S+WE L Sbjct: 623 DSEQKLE-----HAMSKWEEL 638 >lin2179 [R] COG0488 ATPase components of ABC transporters with duplicated ATPase domains Length = 650 Score = 47.0 bits (110), Expect = 7e-06, Method: Compositional matrix adjust. Identities = 27/80 (33%), Positives = 52/80 (65%) Query: 10 LSYEAEKQAKREERNRQRRISELEENIAVLEEDIARIENEMTRPEVFQDYVALQEHESEL 69 L+Y+ EK+ ++ R R+R++ E+E+++ ++ IA +E ++T+PE+FQD+ E EL Sbjct: 567 LNYQEEKEQQKLLRQRKRKLEEVEKSMEETDKKIAELELQLTKPEIFQDHEKAFEITQEL 626 Query: 70 KLKKEQLGNLFSEWENLADE 89 + K + L EWE +++E Sbjct: 627 DVVKARGEKLMEEWEKVSEE 646 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.306 0.125 0.325 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 6,042,148 Number of extensions: 187060 Number of successful extensions: 446 Number of sequences better than 1.0e-05: 4 Number of HSP's gapped: 447 Number of HSP's successfully gapped: 4 Length of query: 89 Length of database: 38,378,431 Length adjustment: 59 Effective length of query: 30 Effective length of database: 31,573,725 Effective search space: 947211750 Effective search space used: 947211750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 43 (22.0 bits) S2: 109 (46.6 bits)