BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0370.0020.p (257 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0553 [U] COG0805 Sec-independent protein secretion pathway com... 204 7e-53 BS_ydiJ [U] COG0805 Sec-independent protein secretion pathway co... 203 2e-52 BS_ycbT [U] COG0805 Sec-independent protein secretion pathway co... 179 3e-45 BH3906 [U] COG0805 Sec-independent protein secretion pathway com... 155 7e-38 SSO0484 [U] COG0805 Sec-independent protein secretion pathway co... 77 4e-14 >BH0553 [U] COG0805 Sec-independent protein secretion pathway component TatC Length = 253 Score = 204 bits (520), Expect = 7e-53, Method: Compositional matrix adjust. Identities = 104/254 (40%), Positives = 157/254 (61%), Gaps = 1/254 (0%) Query: 3 LEAEEMSVVDHLTELRRRIIYXXXXXXXXXXXXXXXXKPIYLYLIHADMAQGFVLHAFSF 62 + +MS++DH+ ELRRRI+ P+ YL A AQ ++AF Sbjct: 1 MNERDMSLMDHIAELRRRILIIVVFFVIALVVGFFLATPMITYLQGAPTAQDLPMNAFKL 60 Query: 63 WDGIGMYVKIAMAVSLAVSVPFIVYQLWAFISPGLRPVERSAALRYVPYVFVLFILGIAF 122 D + +Y+ A + + P I+YQLWAF+SPGL ER A L Y+P F LF+ G++F Sbjct: 61 TDPLRVYMTFAFTSAFILVFPIILYQLWAFVSPGLHENERKATLAYIPIAFFLFLGGLSF 120 Query: 123 AYYIVFPMALSFTITITRSMGLEETYGIAQYFSFMFSLVIPLALLFELPLVVMFLTKLRI 182 AY+I+FP + F + + + E YGI +YF+F+F + +P +LF+LP+VVMFLT+L I Sbjct: 121 AYFILFPFLIQFIGGLAERLHINELYGINEYFTFLFQITMPFGVLFQLPVVVMFLTRLGI 180 Query: 183 LNPLRLRKMRRYAYFALVFIAVVITPPDFISDFLVTIPLLILYEFSVFLSSFVYRKQLAM 242 + P LR +R+YA+F L+ +A ITPP+ IS +VT+PLL+LYEFS+++S YRK + Sbjct: 181 VTPEFLRSVRKYAFFVLLVVAGFITPPELISHLMVTVPLLLLYEFSIWVSHLTYRKVQKL 240 Query: 243 DRELEARYVRKEES 256 ++ + Y R+EE Sbjct: 241 EKLRQEEY-RQEEG 253 >BS_ydiJ [U] COG0805 Sec-independent protein secretion pathway component TatC Length = 254 Score = 203 bits (517), Expect = 2e-52, Method: Compositional matrix adjust. Identities = 98/249 (39%), Positives = 159/249 (63%), Gaps = 3/249 (1%) Query: 3 LEAEEMSVVDHLTELRRRIIYXXXXXXXXXXXXXXXXKPIYLYLIHADMAQGFVLHAFSF 62 ++ +MS+++H+ ELR+R++ KPI +YL D A+ L+AF+ Sbjct: 4 MKVNQMSLLEHIAELRKRLLIVALAFVVFFIAGFFLAKPIIVYLQETDEAKQLTLNAFNL 63 Query: 63 WDGIGMYVKIAMAVSLAVSVPFIVYQLWAFISPGLRPVERSAALRYVPYVFVLFILGIAF 122 D + ++++ A + + ++ P I+YQLWAF+SPGL ER L Y+P +LF+ G++F Sbjct: 64 TDPLYVFMQFAFIIGIVLTSPVILYQLWAFVSPGLYEKERKVTLSYIPVSILLFLAGLSF 123 Query: 123 AYYIVFPMALSFTITITRSMGLEETYGIAQYFSFMFSLVIPLALLFELPLVVMFLTKLRI 182 +YYI+FP + F I++ + + + GI +YF F+ L IP LLF++P+++MFLT+L I Sbjct: 124 SYYILFPFVVDFMKRISQDLNVNQVIGINEYFHFLLQLTIPFGLLFQMPVILMFLTRLGI 183 Query: 183 LNPLRLRKMRRYAYFALVFIAVVITPPDFISDFLVTIPLLILYEFSVFLSSFVYR---KQ 239 + P+ L K+R+YAYF L+ IA +ITPP+ +S +VT+PLLILYE S+ +S YR K Sbjct: 184 VTPMFLAKIRKYAYFTLLVIAALITPPELLSHMMVTVPLLILYEISILISKAAYRKAQKS 243 Query: 240 LAMDRELEA 248 A DR++ + Sbjct: 244 SAADRDVSS 252 >BS_ycbT [U] COG0805 Sec-independent protein secretion pathway component TatC Length = 356 Score = 179 bits (455), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 19/241 (7%), Positives = 38/241 (15%), Gaps = 7/241 (2%) Query: 3 LEAEEMSVVDHLTELRRRIIYXXXXXXXXXXXXXXXXKPIYLYLIHADMAQGFVLHAFSF 62 ++ L L + Y+ + + + Sbjct: 1 MQIIFGEKCVSLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYIGV-NFGVKTLEEEAN- 58 Query: 63 WDGIGMYVKIAMAVSLAVSVPFIVYQLWAFISPGLRPVERSAALRYV---PYVFVLFILG 119 + K S S + + Sbjct: 59 -TAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVYLGDNWYKINDYLAAK 117 Query: 120 IAFAYYIVFPMALSFTITITRSMGLEETYGIAQYFSFMFSLVIPLALLFELPLVVMFLTK 179 + P A+ F + + F L ++ F+ Sbjct: 118 VLLQVKGSSPTAVPF-ENVGTGGDTRWHICDPGGQRLGGQGASGNSGSFSLKILQPFVGS 176 Query: 180 LRILNPLRLRKMRRYAYFALVFIAVVITPPDFISDFLVTIPLLILYEFSVFLSSFVYRKQ 239 + I R Y A TP L + Sbjct: 177 VVIPPMALARLYECYNIPAGDSCTTTGTPVLVYYLSGTINSLGSCSVNAGETIEVDLGDV 236 Query: 240 L 240 Sbjct: 237 F 237 >BH3906 [U] COG0805 Sec-independent protein secretion pathway component TatC Length = 219 Score = 155 bits (391), Expect = 7e-38, Method: Compositional matrix adjust. Identities = 90/200 (45%), Positives = 126/200 (63%), Gaps = 4/200 (2%) Query: 40 KPIYLYLIHADMAQGFVLHAFSFWDGIGMYVKIAMAVSLAVSVPFIVYQLWAFISPGLRP 99 K +YLYL+ + L D + +Y+ IA ++ AV++P YQ+W F+ PGL Sbjct: 16 KDVYLYLVKELDGK---LALLGPGDILWVYMTIAAVLATAVTIPIAAYQVWRFVQPGLTK 72 Query: 100 VERSAALRYVPYVFVLFILGIAFAYYIVFPMALSFTITITRSMGLEETYGIAQYFSFMFS 159 ER A L ++P +F FI GIAF Y+++FP+ L+F + E + +YF FM Sbjct: 73 GERQATLVFIPGLFFFFIAGIAFGYFVLFPLVLTFLSNLAGDQ-FETMFTAEKYFQFMLH 131 Query: 160 LVIPLALLFELPLVVMFLTKLRILNPLRLRKMRRYAYFALVFIAVVITPPDFISDFLVTI 219 L +P LFE+P V+MFLTKL +L P +L K R+YAYF L+ ++VVITPPDF+SD LV + Sbjct: 132 LTLPFGFLFEMPAVIMFLTKLGVLTPEKLSKGRKYAYFLLIILSVVITPPDFLSDVLVIV 191 Query: 220 PLLILYEFSVFLSSFVYRKQ 239 PL ILYE SV LS +YRK+ Sbjct: 192 PLFILYEISVSLSKLIYRKK 211 >SSO0484 [U] COG0805 Sec-independent protein secretion pathway component TatC Length = 294 Score = 76.6 bits (187), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 54/197 (27%), Positives = 104/197 (52%), Gaps = 4/197 (2%) Query: 47 IHADMAQGFVLHAFSFWDGIGMYVKIAMAVSLAVSVPFIVYQLWAFISPGLRPVERSAAL 106 I+ ++ +G L + +D + I++ +++ ++P IV ++WAF++PGL E+ Sbjct: 87 IYNELPKGVKLIIINLFDPLFASFYISLYLAIFTTIPIIVREIWAFVAPGLYEHEKKLFK 146 Query: 107 RYVPYVFVLFILGIAFAYYIVFPMALSFTITITRSMG--LEETYGIAQYFSFMFSLVIPL 164 + F+LF LG AFAY+++ P+ L + ++G +E T G+ + S + +L+I Sbjct: 147 SIIFPAFLLFALGSAFAYFLLIPLMLRIILLYATALGSAVEPTLGLRSFVSTVMTLLIAT 206 Query: 165 ALLFELPLVVMFLTKLRILNPLRLRKMRRYAYFALVFIAVVITP--PDFISDFLVTIPLL 222 L FELPL++ LT + ++ + K R+ IA +I+P I + ++ I L Sbjct: 207 GLSFELPLIMGGLTGVGVVKSITWLKNWRWGVLVSFIIAWIISPGTTGGIIETVIGITLS 266 Query: 223 ILYEFSVFLSSFVYRKQ 239 LY +S F+ + + Sbjct: 267 TLYFIGALISKFMEKGR 283 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.335 0.147 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,726,805 Number of extensions: 577024 Number of successful extensions: 2119 Number of sequences better than 1.0e-05: 14 Number of HSP's gapped: 2105 Number of HSP's successfully gapped: 15 Length of query: 257 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 153 Effective length of database: 26,383,695 Effective search space: 4036705335 Effective search space used: 4036705335 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (21.6 bits) S2: 114 (48.5 bits)